Biological activity of geopropolis produced by Partamona cupira (Meliponinae, Apidae) in the semiarid of the Brazilian northeast

Jael Soares Batista,Wesley Lyeverton Correia Ribeiro,Francisco Herbeson Aquino Silva,Luciana Cristina Borges Fernandes,Kizzy Millenn De Freitas Mendonça Costa,Natanael Silva Félix,Geysa Almeida Viana,Jardel Bezerra Da Silva,Wanderson Lucas Alves Dos Santos,Tiago Da Silva Teófilo

doi:10.33448/rsd-v9i11.9644

Abstract

Research on the chemical composition and pharmacological activities of geopropolis produced by stingless bees (Hymenoptera, Apidae, Meliponini) may contribute to expand its use of propolis-based formulations in the clinical context. Thus he study aimed to evaluate the chemical composition and biological activity of the hydroethanolic extract (HEG) of the geopropolis of Partamona cupira, obtained in the semiarid region of northeast Brazil. Chemical analyses of HEG were carried out using HPLC-DADESI-MS/MS. The antioxidant activity of extracts was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay method and cytotoxic activity by the in vitro MTT method [brometo de 3- (4.5dimetiltiazol-2-il)-2.5-difeniltetrazolio]. The antibacterial activity of the HEG was evaluated through the disc-diffusion test on agar and measurement of the promoted by the extract in different concentrations. The genoprotective potential of the HEG was evaluated through the comet assay on fibroblasts of L929, co-treated with the extract and submitted to genotoxicity induction with H2O2. We also investigated the healing effect of the cream containing geopropolis (10%) on experimental skin wounds in Wistar rats. The HEG presented in its composition phenolic compounds of high biological activity, as well as revealed high antioxidant activity and promoted genoprotective effect by reducing DNA damage from L929 fibroblasts. The HEG presented antimicrobial activity promoting inhibition of S. aureus, S. pyogenes, E. coli and E. aerogenes. The topical use of the cream containing geopropolis promoted wound closure and faster reepithelialization in relation to the control group, in addition to a less intense inflammatory reaction, increased fibroblastic proliferation and collagen deposition.

Highlights

The bees of the subfamily Meliponinae (Hymenoptera, Apidae) present a wide geographical distribution in Brazil, with more than 250 species, distributed in 27 genera (Silveira et al, 2002)
Aiming to seek new knowledge about the quality of products produced by bee species belonging to the subfamily Meliponinae was structured and this study aimed to assess the chemical composition and biological activity the hydroethanolic extract of the geopropolis of the stingless bee Partamona cupira obtained in the semiarid do northeast do Brazil
The results obtained in this study showed that the concentrations of 100 mg.mL-1 provided excellent results, since the averages of the inhibition halos promoted for the bacteria S. aureus, S. pyogenes and E. coli did not differ significantly (P > 0.05) from the halos promoted by the control antibiotic

Summary

Introduction

The bees of the subfamily Meliponinae (Hymenoptera, Apidae) present a wide geographical distribution in Brazil, with more than 250 species, distributed in 27 genera (Silveira et al, 2002). Among the bees belonging to the subfamily Meliponinae, Partamona cupira stands out, which frequently occurs in areas of dry vegetation of the caatinga biome of Northeast Brazil (Silveira et al, 2002). They are docile bees that build their hives in pre-existing cavities, which are difficult to access and make it difficult for predators to enter, such as termite mounds or openings in trees (Hrncir et al, 2017).

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