Nutrition Exercise Research Articles

Ocular Manifestations of Systemic Hypertension: Its Management and Prevention

Hypertension is identified when blood pressure is higher than 140/90 mm of Hg. It is in public health concern in the form of systemic disease as well as in ocular manifestations. The main site for ocular changes in hypertension is retina which shows marked arteriolar attenuation, hemorrhages, oedema and swelling of the optic disc. If further uncontrolled vision threatening conditions like revascularization and even retinal detachment may occur in later stages. Other common ocular manifestations are subconjunctival hemorrhages, strabismus, ptosis and cranial verve palsies. Most of the recent treatment is focused in retinal laser therapy and intravitreal injection of anti VEGFs agents. To prevent the ocular manifestations blood pressure should be in strict control with sodium and salt restriction in diet. Adequate physical exercise and proper nutrition can also play crucial role for its management.

The Effect of Comprehensive Tele-Education in Patients with Diabetes Mellitus: A Randomized Controlled Trial

Aim: This study aimed to investigate the effectiveness of remote education given via telehealth to individuals diagnosed with Type 2
 diabetes on exercise goals, physical activity, nutrition, medication habits and diabetes problems.
 Material and Methods: A randomized controlled trial was conducted with a total of 30 patients with diabetes mellitus (15 tele-education,
 15 routine education). Individuals were educated about general information about diabetes, nutrition therapy, exercise, physical activity,
 drug therapy, and acute/chronic complications. Participants were evaluated with the Mini Nutritional Assessment, International Physical
 Activity Questionnaire, Goal Orientation in Exercise Measurement, Modified Morisky Medication Adherence Scale and Problem Areas
 in Diabetes Scale.
 Results: In terms of MNA scores, only the decrease in the score of routine rehabilitation was significant (p=0.041). The MNA score did
 not differ between the tele-education and routine rehabilitation groups (p>0.05). Finally, the education program did not affect the other
 scores (p>0.05).
 Conclusion: The study results showed that tele-education can only positively affect nutrition. However, both tele-education and routine
 training provided similar and ineffective results. Future studies could focus on remote education programs delivered through different
 educational methods.

Functional capacity and quality of life improvement in stable chronic obstructive pulmonary disease (COPD) patients following physical exercise and chicken egg white supplementation.

The pillars of comprehensive pulmonary rehabilitation program for chronic obstructive pulmonary disease (COPD) patients include physical exercise and good nutrition. The aim of this study was to evaluate the efficacy of pulmonary rehabilitation, which included physical exercise and chicken egg white supplementation, on the quality of life (QoL) and functional capacity among patients with stable COPD. The COPD patients were enrolled prospectively in this quasi-experimental study and completed a 12-week smartphone-guided home-based physical exercise program that comprised strength and resistance training three times per week for 30 minutes each session. Participants were divided into two groups: the control group who underwent physical exercise only, and the intervention group who had physical exercise and chicken egg white supplementation as a protein source. Patient characteristics including sex, age, nutritional status, comorbidities, smoking status, and obstruction severity, were evaluated. The COPD assessment test (CAT) score and six-minute walk test (6MWT) were used as the parameters to evaluate QoL and functional capacity, respectively. Of the total 50 patients included in the study, 12 were excluded due to follow-up and adherence problems. Our data indicated there were significant CAT score reduction and 6MWT improvement in both control and intervention groups after 12 weeks compared to baseline data. However, reduction of mean CAT score was higher in intervention compared to control group (-13.47±6.49 vs -5.42±5.07, p<0.001). In addition, the improvement of 6MWT was also higher in intervention group compared to control group (145.47±69.2 vs 32.42±17.3 meters, p<0.001). In conclusion, chicken egg white supplement to male patients with stable COPD who exercise with resistance and strength training could improve the QoL and functional capacity.

From Chronodisruption to Sarcopenia: The Therapeutic Potential of Melatonin.

Sarcopenia is an age-related condition that involves a progressive decline in muscle mass and function, leading to increased risk of falls, frailty, and mortality. Although the exact mechanisms are not fully understood, aging-related processes like inflammation, oxidative stress, reduced mitochondrial capacity, and cell apoptosis contribute to this decline. Disruption of the circadian system with age may initiate these pathways in skeletal muscle, preceding the onset of sarcopenia. At present, there is no pharmacological treatment for sarcopenia, only resistance exercise and proper nutrition may delay its onset. Melatonin, derived from tryptophan, emerges as an exceptional candidate for treating sarcopenia due to its chronobiotic, antioxidant, and anti-inflammatory properties. Its impact on mitochondria and organelle, where it is synthesized and crucial in aging skeletal muscle, further highlights its potential. In this review, we discuss the influence of clock genes in muscular aging, with special reference to peripheral clock genes in the skeletal muscle, as well as their relationship with melatonin, which is proposed as a potential therapy against sarcopenia.

INTEGRATION OF HEALTH-SAVING TECHNOLOGIES INTO THE EDUCATIONAL PROCESS OF STUDENTS OF NATURAL SCIENCES

The article proposes a number of methods for integrating health-saving technologies into the educational process of students of natural sciences. It describes the use of interactive teaching methods, the inclusion of health-saving topics in the curriculum, the use of the latest technologies, the organization of special trainings and the introduction of health-saving programs at universities.&#x0D; These methods not only improve students' physical and psychological health, but also help develop their social and communication skills. The use of health-saving technologies in the educational process can increase students' motivation to learn, improve their teamwork and provide them with a healthy lifestyle, which in turn has a positive impact on their future careers. The article will be useful for all employees of educational institutions who wish to expand their knowledge of health-saving technologies and their application in the educational process.&#x0D; The integration of health-saving technologies into the educational process of students is an important step in the formation of a healthy lifestyle. This allows students to understand that health is not only the absence of diseases, but also a state of physical, mental and social well-being.&#x0D; In today's world, humanity faces increasing threats such as pandemics, stress, poor nutrition and lack of exercise. Ensuring the health of students is an important factor for successful learning and development, therefore, the inclusion of health-saving technologies in the educational process can help prepare future specialists who will work in health-saving fields.&#x0D; Therefore, the integration of health-saving technologies into the educational process of students is a relevant topic for research, since ensuring the health of students is a key factor for their successful learning and future development. The implementation of such integration can help prepare future specialists in health care fields, thereby ensuring the appropriate level of health in society.&#x0D; The purpose of this article is to investigate the question of the inclusion of health-saving technologies in the educational process of students of natural sciences, as well as to argue the necessity of this step in the modern world. The work will consider various aspects of this issue, such as the impact of health-saving technologies on the health of students, their impact on the quality of the educational process and on the development of future specialists in natural sciences.&#x0D; The subject of the study is the process of integrating health-saving technologies into the educational process of students of natural sciences and its impact on the health and academic success of students. The study also covers the justification of the importance of health-saving technologies in the modern world and their importance for the training of future specialists in health-saving fields.&#x0D; The object of research is health-saving technologies and their impact on the health of young people in general, as well as their role in the modern world and in the process of training future specialists in health-saving fields.

The Relationship Between Exercise Addiction Levels and Healthy Nutrition Levels of Students Studying at the Faculty of Health Sciences

Introduction: Exercise is planned, structured, voluntary, continuous activities aimed at improving one or more elements of physical fitness. One aspect of healthy lifestyle behaviors is nutrition, while another aspect is exercise and physical activity. Many studies in the literature emphasize the physical, psychological, and physiological benefits of regular physical exercise. Therefore, in our study, we aimed to investigate the relationship between exercise addiction levels and healthy nutrition levels of students studying at the faculty of health sciences.Method: The study was conducted on 133 students studying at the faculty of health sciences. Participants' demographic and income information was recorded. Exercise dependence levels were evaluated with the Exercise Dependence Scale, and nutritional levels were evaluated with the Nutrition Exercise Behavior Scale.Results: Average age and body mass index of the participants, respectively; It was 21.83±4.21 and 18.13±3.09. It was found that there was a correlation between the exercise addiction levels of the participants and their healthy eating habits (p&lt;0.001-r=0.386). In addition, it was found that there was no significant correlation with the subscale evaluating unhealthy nutrition parameters (p=0.401-r=0.073).Conclusion: It has been observed that there is a positive correlation between exercise addiction and healthy nutrition levels of students studying at the faculty of health sciences. Accordingly, it is seen that more attention is paid to the level of exercise and healthy nutrition. We believe that exercise should be an integral part of daily activities for a healthy diet.

Physical performance during energy deficiency in humans: An evolutionary perspective

Energy deficiency profoundly disrupts normal endocrinology, metabolism, and physiology, resulting in an orchestrated response for energy preservation. As such, despite energy deficit is typically thought as positive for weight-loss and treatment of cardiometabolic diseases during the current obesity pandemic, in the context of contemporary sports and exercise nutrition, chronic energy deficiency is associated to negative health and athletic performance consequences. However, the evidence of energy deficit negatively affecting physical capacity and sports performance is unclear. While severe energy deficiency can negatively affect physical capacity, humans can also improve aerobic fitness and strength while facing significant energy deficit. Many athletes, also, compete at an elite and world-class level despite showing clear signs of energy deficiency. Maintenance of high physical capacity despite the suppression of energetically demanding physiological traits seems paradoxical when an evolutionary viewpoint is not considered. Humans have evolved facing intermittent periods of food scarcity in their natural habitat and are able to thrive in it. In the current perspective it is argued that when facing limited energy availability, maintenance of locomotion and physical capacity are of high priority given that they are essential for food procurement for survival in the habitat where humans evolved. When energetic resources are limited, energy may be allocated to tasks essential for survival (e.g. locomotion) while minimising energy allocation to traits that are not (e.g. growth and reproduction). The current perspective provides a model of energy allocation during energy scarcity supported by observation of physiological and metabolic responses that are congruent with this paradigm.

Effectiveness and clinical application of multidisciplinary combined exercise and nutrition intervention for sarcopenic older adults with metabolic syndrome: study protocol for a multicentre randomised controlled trial

IntroductionAmong chronic diseases affecting older adults, metabolic syndrome (MetS) is known to be closely related to sarcopenia. Insulin resistance may play a key role in the increased frequency of sarcopenia...

A computational model of the effects of macronutrients absorption and physical exercise on hormonal regulation and metabolic homeostasis

Regular physical exercise and appropriate nutrition affect metabolic and hormonal responses and may reduce the risk of developing chronic non-communicable diseases such as high blood pressure, ischemic stroke, coronary heart disease, some types of cancer, and type 2 diabetes mellitus. Computational models describing the metabolic and hormonal changes due to the synergistic action of exercise and meal intake are, to date, scarce and mostly focussed on glucose absorption, ignoring the contribution of the other macronutrients. We here describe a model of nutrient intake, stomach emptying, and absorption of macronutrients in the gastrointestinal tract during and after the ingestion of a mixed meal, including the contribution of proteins and fats. We integrated this effort to our previous work in which we modeled the effects of a bout of physical exercise on metabolic homeostasis. We validated the computational model with reliable data from the literature. The simulations are overall physiologically consistent and helpful in describing the metabolic changes due to everyday life stimuli such as multiple mixed meals and variable periods of physical exercise over prolonged periods of time. This computational model may be used to design virtual cohorts of subjects differing in sex, age, height, weight, and fitness status, for specialized in silico challenge studies aimed at designing exercise and nutrition schemes to support health.

AGE AND OBESITY INTERFERES IN MUSCLE HYPERTROPHY IN RATS UNDERGOING HIIT TRAINING

Sarcopenic obesity is a condition characterized by the simultaneous presence of obesity andsarcopenia, which is the loss of muscle mass and function. Is a growing concern, especiallyamong the elderly population, and it is important to address both obesity and sarcopeniathrough lifestyle modifications, such as exercise and proper nutrition, to improve overallhealth and quality of life. Our study aimed to evaluate the effect of HIIT training on themuscle fiber area of the EDL muscle in obese rats of different ages. For that, Wistar rats, 7and 12 months old, submitted or not to a HFD diet were subjected to HIIT training, 3 times aweek, on alternate days, for 8 consecutive weeks. After the euthanasia EDL muscles wascollected and processed histologically. Our data showed that HIIT training was able topromote muscle hypertrophy even with consumption of this diet, indicating that practicingthis exercise can stimulate protein synthesis even under conditions of obesity.

The Past, Present, and Future of Equine Science.

A core group of 27 equine nutritionists and physiologists joined together in the late 1960s to formally address and enhance the direction of equine research, creating the Equine Nutrition and Physiology Society. In 2003, that growing society transformed into the Equine Science Society, which now serves as the preeminent, internationally recognized scientific equine organization. In recent years, it has been appreciated that equine science encompasses a wide range of focus areas, including exercise science, nutrition, genetics, reproductive physiology, teaching and extension, production and management, and mix of other specialties, qualified as biosciences. Additionally, trainees are highly valued in the society, with the clear understanding that young people are the future of equine science. Amongst tightening budgets, equine researchers must focus on timely dissemination of high-quality research studies and development of strong, interdisciplinary, cross-species, and multi-institutional collaborations to ensure sustainability of academic research programs. With a little creativity, equine science will continue to thrive for the betterment of the horse and all involved in the equine industry.

The Final Pathway of Carbohydrate Oxidation: Revisiting the mLOC

The confirmation of the Intracellular Lactate Shuttle proposed by Brooks and colleagues in 1998 revolutionized the way lactate metabolism was studied in the field of exercise physiology, nutrition, metabolism, and medicine. Previous investigations identified the structure of the mitochondrial Lactate Oxidation Complex (mLOC) which includes MCT1, LDH, COX4, and CD147. While the predominant view in research and textbooks was that pyruvate was imported into the mitochondria to be oxidized it wasn’t until 2009 that Jiang and Colleagues elucidated the mitochondrial pyruvate carrier (mPC). Knowing this, many researchers developed their questions around either pyruvate or lactate metabolism, which may have led to confusion in the field since both substrates play an important role in carbohydrate oxidation. This dilemma led us to the hypothesis that the mPC is in fact an extended member of the mLOC. Mixed skeletal muscle and liver were excised and whole blood was collected post-mortem from (n=5) 4-month-old C57B/l6 mice. Tissues were homogenized in an isotonic solution supplemented with a protease and phosphatase inhibitor. Blood was resuspended in H2O to rupture the cell membranes and the lysates (RBC) were used as negative controls. Mitochondria were isolated by differential centrifugation and resuspended in RIPA buffer. Protein concentrations of tissues were determined using a BCA standard. Western blotting was performed by loading 20 ug of each lysate, separated on an SDS-PAGE gel, and transferred to a PVDF membrane. Membranes were blocked and probed for MCT1(1:1000), mPC1 (1:500), COX4(1:1000), LDHA (1:1000). An SDS-PAGE was repeated and the mPC was excised from the gel. A trypsin digest was performed to isolate the peptides and then analyzed by one-dimensional LCMS/MS at the UC Berkeley QB3 proteomics/mass spectrometry lab. For cDNA preparation, RNA was extracted from 15 mg of skeletal muscle using the Qiagen RNeasy Fibrous tissue kit, reverse transcribed, and concentrated using Quibit ssDNA kit. Quantitative PCR was run on a QuantStudio3 using Power SYBR Green chemistry to confirm the presence of mPC1 in skeletal muscle. Our results revealed that mPC1 was expressed in skeletal muscle along with other genes associated with the mLOC. Immunoblots of mitochondrial lysates contained MCT1, mPC1, LDH, and COX4 in both the skeletal muscle and liver mitochondrial fractions. As expected, MCT1 was also present in RBC lysates as the MCTs are present in both the sarcolemma and mitochondrial membrane. Moreover, LCMS/MS analysis confirmed the presence of mPC1 corroborating our immunoblots. Colocalization using immunocytochemistry confirms the revised mLOC structure. Accordingly, both the pyruvate and lactate transporters assist in maintaining the redox balance and varying rates of carbohydrate oxidation during changing physiological conditions. This promising data has implications for a variety of fields ranging from cancer biology to exercise physiology. NIH Grant #1 R01 AG059715-01 This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

SPA THERAPY: A GERIATRIC PERSPECTIVE

Spa therapy refers to the preventative, therapeutic, and rehabilitative use of thermo mineral waters, mud/peloid compresses, and natural gases. Applications are administered in sessions, and treatment regimens are determined in detail. Various modalities, such as exercise interventions, massage, phytotherapy, relaxation techniques, psychotherapy, nutrition, and electrotherapy, can be incorporated into the treatment process. Spa therapy has been effectively used to manage various diseases in many European countries, Japan, and Israel. Due to its natural structure, the difficulties in using drugs that frequently have side effects, and the limitations of many contemporary therapeutic alternatives, it is still a choice for treating a number of chronic conditions, especially rheumatic disorders. Furthermore, the global population is getting older. Considering the comorbidities, organ dysfunctions, and multiple drug use of elderly individuals, non-pharmacological treatment methods such as spa therapy have become more prominent. Spa therapy has been shown to be effective in treating osteoarthritis, balance problems, and chronic pain in older adults. Additionally, it might be chosen for managing psychiatric conditions and mood changes. Alterations in telomere lengths, reduced pain levels, increased cortisol concentrations, and an overall anti-inflammatory influence may mediate spa therapy’s anti-aging effect. Although opinions and assumptions support its anti-aging properties, more research is needed to prove its biological effects.

Cystic fibrosis - Ways to improve the quality of life of patients

Introduction Cystic fibrosis (CF) is a serious, multi-system disease. In the Caucasian population CF is the most common autosomal recessive disease, which happens for 1 person in 3,500 births [1]. In the course of CF, there is a dysfunction of many organs, mainly the respiratory and digestive systems. Symptomatic treatment of cystic fibrosis is very important. The change in daily eating habits, the use of proper breathing techniques and psychotherapy also have an impact on improving the quality of life of patients. In the past patients died at a young age. The progress in the diagnosis and treatment of cystic fibrosis has meant that nowadays more and more people are reaching adulthood. In recent years, the median survival of patients has increased to 46.2 years. [2] Purpose The aim of this review is to present the current state of knowledge about ways to improve the quality and length of life of patients with CF. Methods The literature available in the Pubmed and Google Scholarship databases was reviewed using key phrases. Results Additional methods of treatment, such as: learning the correct breathing technique, oxygen therapy, physical exercise or proper nutrition, significantly improve the living conditions of patients.

Bridging the gap: Evidence-based practice guidelines for sports nutritionists.

Evidence-based practice is a systematic approach to decision-making developed in the 1990s to help healthcare professionals identify and use the best available evidence to guide clinical practice and patient outcomes amid a plethora of information in often challenging, time-constrained circumstances. Today's sports nutrition practitioners face similar challenges, as they must assess and judge the quality of evidence and its appropriateness to their athlete, in the often chaotic, time-pressed environment of professional sport. To this end, we present an adapted version of the evidence-based framework to support practitioners in navigating their way through the deluge of available information and guide their recommendations to athletes whilst also reflecting on their practice experience and skills as evidence-based practitioners, thus, helping to bridge the gap between science and practice in sport and exercise nutrition.

Challenges in the Management of Sarcopenia in the Primary Care Setting: A Scoping Review

Sarcopenia is a disease associated with the loss of muscle mass, strength, and function. It affects the elderly in various ways, such as reduced mobility, compromising their daily activities, and even deteriorating metabolic health. Primary care serves as the first point of contact for patients and plays an important role in health promotion and disease prevention. Hence, this review is conducted to identify the challenges in the management of sarcopenia in the primary care setting. In December 2022, a scoping review was conducted using PubMed, SCOPUS, Web of Science, and a manual search, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria. We used articles that have been written in English, and relevant articles were then screened, duplicates were removed, eligibility criteria were applied, and studies that met the criteria were reviewed. The keywords challenges, management, sarcopenia, and primary care were included. The initial search generated 280 publications, and 11 articles were included after inclusion and exclusion criteria for this review. In this review, challenges in the management of sarcopenia in a primary care setting are reviewed based on the screening and diagnosis. With an increasing aging population, it is important to understand the challenges in the management of sarcopenia in a primary care setting. Identification of elderly at risk of sarcopenia, followed by referring the affected elderly for confirmation of the diagnosis, is essential to preventing the adverse health effects. The initiation of treatment that comprises resistance exercise training and nutrition should not be delayed, as they are salient in the management of sarcopenia.

Pro: Can we influence postoperative outcomes of frail patients after cardiac surgery?

A significant proportion of cardiac surgery patients are frail. The first step to improve outcomes is recognizing that despite reduced postoperative mortality in cardiac surgery, postoperative morbidity remains high, most likely due to more frail patients undergoing surgeries. Moving from a traditional to a patient-oriented outcome paradigm could improve decision-making and help achieve treatment goals relevant to frail patients, such as quicker recovery, symptom control, and strategies to reduce postoperative complications. Frailty recognition can prompt preoperative and postoperative interventions. Exercise programs and nutrition optimization support frail patients in the perioperative period and positively alter outcomes.

Dietary Arginine and Citrulline Supplements for Cardiovascular Health and Athletic Performance: A Narrative Review.

The global market for nutritional supplements (NS) is growing rapidly, and the use of L-arginine (Arg), L-citrulline (Cit), and citrulline malate (CitMal) supplements has been shown to enhance cardiovascular health and athletic performance. Over the past decade, Arg, Cit, and CitMal supplements have received considerable attention from researchers in the field of exercise nutrition, who have investigated their potential effects on hemodynamic function, endothelial function, aerobic and anaerobic capacity, strength, power, and endurance. Previous studies were reviewed to determine the potential impact of Arg, Cit, and CitMal supplements on cardiovascular health and exercise performance. By synthesizing the existing literature, the study aimed to provide insight into the possible uses and limitations of these supplements for these purposes. The results showed that both recreational and trained athletes did not see improved physical performance or increased nitric oxide (NO) synthesis with 0.075 g or 6 g doses of Arg supplement per body weight. However, 2.4 to 6 g of Cit per day for 7 to 16 days of various NSs had a positive impact, increasing NO synthesis, enhancing athletic performance indicators, and reducing feelings of exertion. The effects of an 8 g acute dose of CitMal supplement were inconsistent, and more research is needed to determine its impact on muscle endurance performance. Based on the positive effects reported in previous studies, further testing is warranted in various populations that may benefit from nutritional supplements, including aerobic and anaerobic athletes, resistance-trained individuals, elderly people, and clinical populations, to determine the impact of different doses, timing of ingestion, and long-term and acute effects of Arg, Cit, and CitMal supplements on cardiovascular health and athletic performance.

Mobile health technology, exercise adherence and optimal nutrition post rehabilitation among people with Parkinson’s Disease (mHEXANUT) – a randomized controlled trial protocol

BackgroundAlthough it is well known that regular physical activity and exercise, as well as maintaining adequate nutritional status is important to delaying symptom development and maintaining physical capacity and function in people with Parkinson’s Disease (PD), many are unable to follow self-management recommendations. Active interventions have shown short-term effects, but there is a need for interventions that facilitate self-management over the course of the disease. Until now, no studies have combined exercise and nutritional interventions with an individual self-management approach in PD. Thus, we aim to examine the effect of a six-month mobile health technology(m-health)-based follow-up programme, focusing on self-management in exercise and nutrition, after an in-service interdisciplinary rehabilitation programme.MethodsA single-blinded, two-group randomised controlled trial. Participants are Adults aged 40 or older, with idiopathic PD, Hoehn and Yahr 1–3, living at home. The intervention group receives a monthly, individualized, digital conversation with a PT, combined with use of an activity tracker. People at nutritional risk get additional digital-follow-up from a nutritional specialist. The control group receives usual care. The primary outcome is physical capacity, measured by 6-min walk test (6MWT). Secondary outcomes are nutritional status, Health related quality of life (HRQOL), physical function and exercise adherence. All measurements are performed at baseline, after 3 months and after 6 months. Sample size, based on primary outcome, is set at 100 participants randomized into the two arms, including an estimated 20% drop out.DiscussionThe increasing prevalence of PD globally makes it even more important to develop evidence-based interventions that can increase motivation to stay active, promote adequate nutritional status and improve self-management in people with PD. The individually tailored digital follow-up programme, based on evidence-based practice, has the potential to promote evidence-based decision-making and to empower people with PD to implement exercise and optimal nutrition in their daily lives and, hopefully, increase adherence to exercise and nutritional recommendations.Trial registrationClinicalTrials.gov (NCT04945876). First registration 01.03.2021.

Acute heat stress amplifies exercise-induced metabolomic perturbations and reveals variation in circulating amino acids in endurance-trained males.

What is the central question of this study? Whole-body substrate utilisation is altered during exercise in hot environments, characterised by increased glycolytic metabolism: does heat stress alter the serum metabolome in response to high intensity exercise? What are the main finding and its importance? Alongside increases in glycolytic metabolite abundance, circulating amino acid concentrations are reduced following exercise under heat stress. Prior research has overlooked the impact of heat stress on protein metabolism during exercise, raising important practical implications for protein intake recommendations in the heat. Using untargeted metabolomics, we aimed to characterise the systemic impact of environmental heat stress during exercise. Twenty-three trained male triathletes ( =64.8±9.2mlkgmin-1 ) completed a 30-min exercise test in hot (35°C) and temperate (21°C) conditions. Venous blood samples were collected immediately pre- and post-exercise, and the serum fraction was assessed via untargeted 1 H-NMR metabolomics. Data were analysed via uni- and multivariate analyses to identify differences between conditions. Mean power output was higher in temperate (231±36W) versus hot (223±31W) conditions (P<0.001). Mean heart rate (temperate, 162±10beatsmin-1 , hot, 167±9beatsmin-1 , P<0.001), peak core temperature (Trec ), core temperature change (ΔTrec ) (P<0.001) and peak rating of perceived exertion (P=0.005) were higher in hot versus temperate conditions. Change in metabolite abundance following exercise revealed distinct clustering following multivariate analysis. Six metabolites increased (2-hydroxyvaleric acid, acetate, alanine, glucarate, glucose, lactate) in hot relative to temperate (P<0.05) conditions. Leucine and lysine decreased in both conditions but to a greater extent in temperate conditions (P<0.05). Citrate (P=0.04) was greater in temperate conditions whilst creatinine decreased in hot conditions only (P>0.05). Environmental heat stress increased glycolytic metabolite abundance and led to distinct alterations in the circulating amino acid availability, including increased alanine, glutamine, leucine and isoleucine. The data highlight the need for additional exercise nutrition and metabolism research, specifically focusing on protein requirements for exercise under heat stress.