Ethanolic Leaf Research Articles

Discovery of Active Antibacterial Fractions of Different Plant Part Extracts of clove (Syzigium aromaticum) Against Streptococcus mutans

Nowadays, dental cavities caused by Streptococcus mutans are a major focus of research in Indonesia. While several antibiotics are available to combat this bacterium, concerns about antibiotic resistance have prompted researchers to explore natural remedies. Clove (Syzigium aromaticum) is a commonly studied natural remedy against dental cavities and S. mutans. Among the different parts of the clove plant, clove bud is the most widely used against dental cavities or S. mutans, and the potential of other clove parts has not been thoroughly explored. Identifying which parts of the clove plant have higher concentrations of active ingredients and exhibit the strongest antibacterial activity is important. Therefore, this study evaluated the antibacterial activity of three different parts, i.e., leaf, stems, and buds of the clove plant ethanolic extracts against S. mutans. The ethanolic extracts of clove leaf, stems, and buds were prepared using the maceration method with 70% ethanol, and their activity against S. mutans was tested using the disc diffusion method at three different concentrations (10%, 5%, 2.5% b/v). Fractionation was carried out using hexane and water to obtain two fractions: hexane and water fraction. These fractions were then subjected to antibacterial assays. The ethanolic leaf, stems, and bud extracts exhibited varying antibacterial activity levels. The best activity was observed with the 10% clove bud ethanolic extract, which produced an inhibition zone of 20.83 ± 0.77 mm. The leaf and stem extracts showed inhibition zones of 16.38 ± 3.84 mm and 17.95 ± 5.15 mm, respectively. Furthermore, the hexane-soluble fraction of the clove bud displayed the highest activity with an inhibition zone diameter of 23.7 ± 3.21 mm at 10%. This activity was twice as high as ampicillin, used as the positive control. In conclusion, clove bud remains the best source of antibacterial compounds against S. mutans. Fractionation of the bud extract using hexane can significantly enhance its activity. Further investigation should be conducted to optimize the effectiveness of this active fraction for use as an anti-dental caries treatment.

Investigation of phenolic contents and in vitro biological activities of Inula helenium subsp. pseudohelenium extracts

AbstractThe purpose of the present study was to investigate the phenolic profile, antioxidant capacity, and biological activity of Inula helenium subsp. pseudohelenium extracts, for the first time. Phenolic substances in leaf ethanol (L-EtOH), leaf pure water (L-PW), root ethanol (R-EtOH), and root pure water (R-PW) extracts of I. helenium subsp. pseudohelenium plant were investigated by HPLC. Also, the antimicrobial (hollow agar), antioxidant, antiproliferative, and DNA protective activities of the samples were examined in vitro. Gallic acid was not detected in the extracts, the presence of curcumin was detected only in EtOH extracts. L-EtOH exhibited the best antimicrobial activity. According to the antioxidant results, the L-EtOH extract had an activity profile close to the standard antioxidants in general. It was also found that the L-EtOH extract stabilised the DNA by disrupting the scavenging effect of H2O2+DMSO. According to the antiproliferative activity results, EtOH extracts exhibited the strongest effect, especially against MCF7 cancer cell lines. When evaluated in general terms, it was found that L-EtOH extract showed better activity than other extracts. It is important to conduct more in vivo and in vitro studies after isolating the active components of extracts e.g. L-EtOH, which have good effects in terms of biological activity.

Costus speciosus leaf and seed extracts for wound healing: a comparative evaluation using mice excision wound models

BackgroundThe present study investigated the potential wound healing activity comparisons between ethanol leaf & seed extracts of Costus speciosus topical formulation using mice Excision wound models, compared to Neomycin sulphate ointment as a standard drug. To assess the efficacy of Costus speciosus ointment as a potential alternative to commercially available ointments, a study was conducted using a cohort of 16 healthy male mice, aged between 3 and 4 months. The ointment formulation was prepared utilizing ethanol extracts from both the leaves and seeds, presented in a dosage form for application. This investigation seeks to provide insights into the comparative effectiveness of Costus speciosus ointments in relation to conventional market preparations, with a focus on its potential applicability as an alternative therapeutic option. The parameter measured was wound contraction and epithelization period.ResultThe mice groups treated with seed & leave extracts ointment showed better wound size changes than the negative and standard groups. However, the leaf extract ointment promotes the formation of granulation in tissue, allowing the reepithelization phase to occur more rapidly than seed extract. From the 1st day to the results of the 9th day of the extract indicate that there is a significant increase (P < 0.05) in the percentage of wound contraction in the group. After 21 days the wound was healed fully. These results suggest that ointment of ethanol leaf extract of Costus speciosus could be an answer to facilitate wound healing compared to seed extract, to approve the traditional claims of the plant in wound healing activity.ConclusionThe study concludes that ethanol leaf and seed extracts of Costus speciosus exhibit potential wound healing activity in mice excision wound models. The topical formulation of these extracts significantly enhances wound closure, reduces wound size, and increases tensile strength compared to the control group. The efficacy of the extracts is comparable to the standard drug, Neomycin sulphate ointment. Therefore, the use of Costus speciosus extracts in topical formulations can be considered a promising alternative for the treatment of wounds. However, further studies are needed to determine the safety and efficacy of these extracts in humans.

Cytotoxicity of leaves, stems, and flowers of Kecubung (Datura metel) extracts using the Brine Shrimp Lethality Test (BSLT) method

Brine shrimp (Artemia salina) cytotoxicity assays (BSLT) are one of the commonly used cytotoxic test methods to assess a plant extract's pharmacological activity and toxicity. Thus, this study aimed to examine the toxic levels of Kecubung (Datura metel) leaves extracted using different solvents, namely ethanol and ethyl acetate. The results showed that the LC50 value of flower ethanol, flower ethyl acetate, leaf ethanol, and stem ethyl acetate extracts had an LC50 value 1000, which was included in the toxic category. The LC50 value of flower ethanol is 121.044 ppm, flower ethyl acetate 105.89 ppm, leaf ethanol 639.589 ppm, and stem ethyl acetate 635.276 ppm. Ethanol leaf extract at a concentration of 1000 ppm showed the highest mortality with a percentage of 60% of the total number of A. salina. The flower ethanol extract showed the highest mortality at a concentration of 250 ppm with a percentage of 67% and at a concentration of 1000 ppm with a percentage of 70% of the total number of A. salina. Meanwhile, flower ethyl acetate and stem ethyl acetate extract at a concentration of 1000 ppm obtained 100% mortality of A. salina in the first 6 hours. The result shows that the flower ethyl acetate and stem ethyl acetate extract at a concentration of 1000 ppm is very toxic compared to other concentrations.Keywords:ToxicityArtemia salinaLC50Ethyl acetateEthanol

Acute toxicity test of water and ethanol extract from african leaf (Gym-nanthemum amygdalina Del.) on zebra fish embrio (Danio rerio)

Bitterleaf or African Leaf is a herbal plant empirically used to relieve fever and kidney disease. Pharmacological research reports that the leaves have antibacterial, anti-inflammatory, and antidiabetic properties, and a toxicity test was also conducted to ensure their safety. Therefore, this experiment aimed to obtain LC50 of African Leaf (Gymnanthemum amygdalina Del.) ethanol and water extract with the ZFET (Zebra Fish Embryo Acute Toxicity) method and also to observe the toxicity effect of zebrafish (Danio rario) embryo's morphology after the induction of the extract. The experiment refers to the OECD No.236 of 2013 as a guideline, and it was shown that African Leaf's ethanol extract LC50 values were obtained at 6.3629 ppm. Furthermore, the water extract LC50 value was obtained at 25.0520 ppm, and the African Leaf extract was categorized in the toxic category. The leaf extract has a harmful effect on zebrafish embryos, resulting in malformations of the tail, notochord, pericardium, and yolk sac.

Phenolic Compounds, Organic Acid Profiles and Antioxidant Potential of Salvia verticillata L.

Salvia genus, which is widely used in folk medicine and attracts great attention, is a rich source of polyphenols, which has been the subject of many chemical studies. Leaf ethanol and root ethanol extracts of Salvia verticillata L. plant sampled from Muş region were obtained by using the soxhlet extraction method. Antioxidant profiles of these extracts were defined by five different methods. Although S. verticillata L. leaf extract showed the best activity in all tests, it was lower than the antioxidants we used as standard. The presence of 17 phenolics, 13 organic acids, and sugars in these extracts was screened by HPLC and correlated with their antioxidant potential. In this context, the difference between the organs of the species examined and collected from a region was also revealed. Leaf extracts have been found to be rich in curcumin, which has anti-inflammatory, anti-cancer, and strong antioxidant capacity. It was determined that acetic acid, an organic acid that is also used as a food preservative, was found in very high amounts in root and leaf extracts. According to the results obtained, it can be thought that the phenolic and organic acid contents of S. verticillata L. may be among the parameters responsible for antioxidant activity, and they are also natural sources for pharmacological processes and the food industry.

Phytochemical Composition, Antioxidant and Antimicrobial Potentials of some Indigenous Plants in Umudike, Abia State, Nigeria

Twenty four ethanol leaf and stem bark extracts of 17 indigenous plants were examined for their phytochemical composition, antimicrobial and antioxidant properties. Phytochemical compositions were analysed with GC-MS while antimicrobial activities on Staphylococcus aureus and Pseudomonas aeruginosa were investigated by the agar well diffusion method. The antioxidant activities were determined with Ferric reducing antioxidant power (FRAP), total phenolic content (TPC) and 2, 2,-dihenyl-1-picryhydazyl (DPPH) radical scavenging assays. The antibacterial activity was more towards the gram positive S. aureus than the gram negative P. aeruginosa for all the plant extracts. A wide range of phenolic concentrations among the aqueous plant extracts which varied from 28.04 to 500.26mg GAE per gram were observed. Inhibition percentages of DPPH ranged from 19.13 to 95.77% showing effectiveness in radical scavenging. GC-MS characterization of the plant extracts showed a total of 18 components including alkaloids, flavonoids, phenols, saponins, terpenoids, steroids and glycosides. Irvingia gabonensis leaf (IGL) extract and Tamarind stem bark (TSB) exhibited excellent ferric reducing abilities of 2.11 and 1.56 respectively while Voucanga Africana leaf (VCA) extract indicated the lowest ferric reducing power of 0.50. Extracts of IGL and TSB exhibited the highest antioxidant capacities and therefore could be the main sources of natural antioxidant. An important relationship between total phenolic content was observed showing that the major contributor to the antioxidant properties were phenolic compounds.

The Phytochemical Screening and Anti-cancer activity of Ethanolic Extracts of Selected Mangrove Plants

Background: Mangrove ecosystems have been recognized to include a wide range of secondary metabolites, which are biochemically distinct, resulting in a diverse range of natural compounds with unique bioactivity. They have active metabolites with new chemical structures from a variety of chemical classes.&#x0D; Objectives: This study was undertaken to evaluate the phytochemical screening and cytotoxicity of four major mangrove plants (Excoecaria agallocha L., Acrostichum aureum L., Aegiceras corniculatum L., and Avicennia officinalis L.).&#x0D; Methods: This experimental study was held in the Biochemistry and Molecular Biology Laboratory of Khulna University, Bangladesh, in 2016. At the first phytochemical screening of the selected plants was observed. Then, the bioactivity as preliminary cytotoxic activity was performed using brine shrimp lethality (BSL) bioassay where a significant 50% Lethal Concentration (LC50) was exerted using polar solvent (ethanol) extract of different plant parts (leaf, bark, and stem). Then, Resazurin Cell Viability Assay was performed only for ethanolic leaf and bark extracts of E. agallocha using four standard bacteria (Escherichia coli ATCC 8739, Salmonella typhi ATCC 6539, Salmonella paratyphi ATCC 9150, and Staphylococcus aureus ATCC 25923).&#x0D; Results: The experimental findings showed significantly strong LC50 by ethanolic leaf and bark extracts of E. agallocha and other plants, like A. corniculatum, A. aureum, and A. officinalis showed moderate and negligible cytotoxicity, respectively. Then, the experimental findings showed significantly (P≤0.05) strong IC50 by ethanolic leaf and bark extracts of E. agallocha.&#x0D; Conclusion: The screens employed in this present study are preliminary and advanced assays are needed to verify and reveal further this bioactivity present in those plants, particularly E. agallocha.

Apoptotic and anti-proliferative effects of ethanolic Neem leaf extract on oral squamous cell carcinoma cell line

Apoptotic and anti-proliferative effects of ethanolic Neem leaf extract on oral squamous cell carcinoma cell line

ACTIVITY OF BAY LEAF EXTRACT (Eugenia polyantha) AS ANTI-INFLAMMATORY IN WHITE RAT (Rattus norvegicus)

Background: Inflammation is the body's defense response against foreign body invasion, tissue injury, or both caused by microorganisms, mechanical trauma, chemical compounds, and physical factors. Anti-inflammatory agents can be used to treat inflammation. Bay leaves are one example of a plant that can be used. Ethanol extract and water extract of a bay leaf can be anti-inflammatory because they contain flavonoids. Objectives: The purpose of this study was to see how effective ethanol extract and aqueous extract of bay leaf were as anti-inflammatory agents in mice. Methods: This study employs a systematic literature review approach, descriptive qualitative research, and library research. Results: The most efficacious dose was 150 mg/kg BW at a 70 percent concentration and at an 80 percent concentration for ethanol extract of bay leaves, and 50 mg/kg BW for the aqueous extract of bay leaves. Conclusion: Based on the results of this study, it can be concluded that bay leaf (Eugenia polyantha) ethanol extract and aqueous extract have anti-inflammatory activities&#x0D; Keywords: Anti-inflammatory, Bay Leaf, Diclofenac sodium, Ethanol Extract, Aqueous

GC–MS-Based Metabolites Profiling, In Vitro Antioxidant, Anticancer, and Antimicrobial Properties of Different Solvent Extracts from the Botanical Parts of Micromeria fruticosa (Lamiaceae)

The present study assessed the metabolites and bioactivities of Micromeria fruticosa plant parts extracted with various solvents (ethanol, n-hexane, and water) through the steeping extraction method. Thereafter, the extracts were analyzed using GC-MS. Moreover, the extracts were tested for their antioxidant, antimicrobial, and antitumor activities. The quali-quantitative analysis of Micromeria fruticosa crude extracts revealed the occurrence of 27 secondary metabolites. Some major bioactives identified were menthone, oleamide, pulegone, and menthol. Numerous antioxidant minerals, viz., Fe, Zn, and Mn, were present. The water extract of leaves exhibited the highest DPPH scavenging activity (89.73%), followed by the water extract of flowers (80.07%) at 100 μg/mL. The stems’ water extract demonstrated greater antimicrobial activity against all the bacteria species tested. The ethanolic leaf and aqueous stem extracts exhibited strong activity against C. albicans and E. coli. Flowers’ aqueous extract demonstrated the highest cytostatic effect on the colon cell line by reducing viability, followed by the leaves’ ethanol extract. The extraction solvents influenced the recovery of phytocompounds, and the highest pharmacological activities of the different extracts could be correlated to the presence of additional bioactives. Our results suggest that the Micromeria fruticosa plant is a favorable source of natural products with promising properties for potential nutraceutical and functional food applications.

Potentials of kawayang tinik (Bambusa blumeana) as new source antimicrobial agents

In this time where health is priority and surges of microbial resistance is highly observed within the society, discovering new, effective and sustainable sources of potential pharmacologic products is highly significant. The study explored the antimicrobial potentials of the different parts of Bambusa blumeana (kawayang tinik), a common Philippine bamboo species, against selected pathogenic bacterial and fungal species utilizing minimum inhibitory concentration via agar well diffusion method. Results of the study showed that extracts of B. blumeana, specifically the leaf, rhizome, roots, inner and outer culms, are capable of inhibiting microbial growth at 0.06 to 0.98 mg/ml concentrations. Specifically, the aqueous outer culm extract of B. blumeana proved to be most effective in inhibiting the growth of Pasteurella multocida at 0.24 mg/ml while Staphylococcus aureus and Escherichia coli were most susceptible to ethanolic outer culm extracts at 0.06 mg/ml and 0.12 mg/ml respectively. Bacillus subtilis, on the other hand, was observed to be the most sensitive to ethanolic root extracts at 0.06 mg/ml. Furthermore, Aspergillus niger was observed to be susceptible to ethanolic rhizome extract (0.24 mg/ml) while the ethanolic leaf, roots, inner and outer culms were equally effective in inhibiting Penicillium chrysogenum at 0.98 mg/ml extract concentration. Phytochemical testing further revealed the presence of phenols and flavonoids in the different parts of the bamboo species which further support its potential as a new source of pharmaceutical biocompounds.

Development of Antimicrobial Wound Dressing Bandage using Chromolaena Odorata Leaf Extract

A bandage is a standard of biomaterial used on wounds to protect from infections and to cure wounds. The adhesive bandage also known as sticking plaster protects the wound from friction, bacteria, damage, and dirt. In this study, the leaf extracts of plant (Chromolaena odorata) coated on textiles are being used as a potential drug to promote wound healing. The phytochemical screening and antimicrobial activities of the ethanolic plant leaf extracts was carried out. The leaf extracts were coated on non-woven viscose rayon fabric using Pad-dry cure method. The physical and biological parameters of the coated fabric were analyzed to determine the process of wound healing. The parameters of pH and absorbency were evaluated using the standard protocols obtained from medical textiles. The antimicrobial efficiency (standard protocol AATCC 100), cytotoxicity (MTT assay) and in vitro scratch wound assay using cell lines was studied. The results showed that developed herbal coated bio-bandage featured all the characteristics for ideal dressing. Therefore, it can be promoted as novel bio-bandage for the healing of wounds.

Analgesic, anti-inflammatory and acute oral toxicity profile of leaf and bark extracts of Albizia procera

BackgroundPain and inflammation are associatory events in cancer, diabetes, cardiovascular diseases, arthritis and other chronic diseases. Corticosteroids, non-steroidal anti-inflammatory drugs exert potential side effects on long term use. This study was aimed to investigate the acute oral toxicity, anti-inflammatory and analgesic activities of leaf and bark extracts of Albizia procera in experimental animal models.MethodsEthyl acetate, ethanol, and hydroalcoholic extracts of Albizia procera (leaf and bark) were subjected for acute oral toxicity, anti-inflammatory and analgesic screening. Carrageenan and cotton pellet granuloma models were used to assess acute and chronic anti-inflammatory effects, respectively. Intraplanar formalin test was used to assess the analgesic activity.ResultsAll the extracts of Albizia procera were found to be well-tolerated up to 2000 mg/kg in female rats. Ethanolic leaf (ETLE) and bark (ETBE) of Albizia procera showed anti-inflammatory actions. But, only ETBE produced significant protection in chronic inflammation and analgesic activity.ConclusionIn summary, Albizia procera possess significant anti-inflammatory and analgesic properties. This study adds evidence on the traditional use of Albizia procera plant for treating painful inflammatory disorders.

Evaluation of the phenolic compounds and the antioxidant potentials of Vitex agnus-castus L. leaves and fruits

Abstract Objectives In this study, we aim at deciphering the phenolic content of Vitex agnus-castus L. leaf and fruit extracts prepared with different methods and relate it to their antioxidant activity. Methods In this study, phenolic compounds and the antioxidant potential of the ethanol fruit and leaf extracts of V. agnus-castus L. (Chaste tree) were evaluated spectrometrically. Furthermore, selected polyphenols, i.e., chlorogenic acid and rutin, were determined by the HPLC-DAD method qualitatively and quantitatively. Results The results obtained from leaf and fruit extracts were compared with a commercial product (CP) containing the fruit extract of V. agnus-castus. Leaf extract was found to be richer in flavonoids when compared to the fruit counterparts. Accordingly, they also showed higher antioxidant activity. Conclusions Extracts prepared here can be considered as promising antioxidant agents for future therapeutic formulations.

In vivo Toxicity Evaluation of Ethanolic Extract From Two Mangrove Plants

Background: Mangrove plants, often possessing a unique collection of metabolites, have extensively been used for the primary healthcare of coastal residents. Objectives: To determine the safety level and enrich the scientific data, the present study aimed to investigate the toxicity of Avicennia officinalis and Excoecaria agallocha. Methods: Ethanolic leaf and stem extracts were evaluated for cytotoxicity by brine shrimp lethality assay. The obtained extracts were administered to Swiss albino in a single dose (200, 400, 800, 1600, &amp; 3200 mg/kg body weight) by oral-gavage for acute toxicity assay. Furthermore, systematic observation was performed by close monitoring for any toxic manifestations and mortality after dosing for the first 4 h, at 24 h and twice daily for 6 days. Evaluating the adverse effects were estimated by comparing the test groups with the controls. After sacrificing all group animals, relative organ weight was measured and histopathological analysis was conducted. Results: Having Lethal Concentration (LC50) of 44.66 µg/mL, E. agallocha leaf was found with the highest toxicity against brine shrimp nauplii. The toxicological study data demonstrated no death and noticeable change in behavioral patterns in the test mice groups, compared with the control group. Moreover, no significant (P&gt;0.05) differences were found in body weight and relative organ weights, compared to the controls. The histological structures of the liver in the treated mice displayed regular tissue configurations similar to the control group. Conclusion: In this study, the mice model exhibited no harmful effects; thus, the reported results indicated that the ethanolic extracts of leaf and stem of these two mangrove plants are safe for therapeutic use. Further long-term toxicological impact of the extracts should be determined for well-founded confirmation.

Larvicidal activity of ethanolic leaf and seed extract of Croton tiglium against Aedes aegypti

Aim and objectives: Mosquitoes are the transmitters of serious human diseases that cause millions of deaths every year. The use of synthetic insecticides causes harmful side effects to the environment and human health. So a natural alternative way is needed to solve this problem. Extracts from the various parts of plants like leaves, seeds, barks and roots can be used to control mosquitoes. This study aims to evaluate the larvicidal activity of ethanolic seed and leaf extracts of a traditionally used medicinal plant Croton tiglium, against Aedes aegypti, the vector of dengue fever. Material and methods: In this study, extracts from the dried leaf and seed powder of the Croton tiglium plant were extracted using ethanol in a soxhlet apparatus. Different concentrations were prepared from the extracts. Third instar larvae of Aedes aegypti were collected from the mosquito culture laboratory of the Life Sciences Department of Dibrugarh University. Prepared concentrations of the extracts were tested against the larvae of Aedes aegypti to evaluate the larvicidal activity by calculating percent mortality. Result: The ethanolic leaf and seed extracts of the selected plant were found effective against the larvae of Aedes aegypti. The 250 ppm concentration of both leaf and seed extracts showed the highest mortality of mosquito larva. The seed extract of this plant was found more effective in killing mosquito larva. Conclusion: From the study, it can be concluded that the ethanolic leaf and seed extracts of Croton tiglium possess larvicidal activity against Aedes aegypti. Hence, it could be useful in controlling mosquitoes without any harmful effects. However, further study and investigation will be needed to isolate and characterize bioactive components from this plant with larvicidal activity and the development of drugs for dengue from the components

The phytochemical constituents and antibacterial activity of methanolic and ethanolic leaf and stem extracts of Eucalyptus torelliana, Nigeria

The use of traditional medicines has been observed to increase globally. The search for new antimicrobial agents has increased as a result of increase in microbial infections as well as antimicrobial resistance. The phytochemical and antibacterial activities of ethanolic and methanolic extract of leaves and stem of Eucalyptus torelliana was investigated to detect the presence of secondary metabolites and also evaluate their antibacterial potential. The phytochemical constituents of the powdered leaves and stem of Eucalyptus torelliana were determined using standard methods. The antibacterial susceptibility of bacteria from different sources to the leaves and stem extract was determined using agar diffusion method. Minimum inhibitory concentrations, MIC of the extracts were also determined. Phytochemical screening of Eucalyptus torelliana yielded glycosides, reducing sugars, condensed tannins and terpenoids in both leaf and stem extract while saponins were found only in the leaves extract. The ethanolic and methanolic extracts of Eucalyptus torelliana had antibacterial activities at 20mg/ml and 10mg/ml especially at 20mg/ml where it showed significant difference in their activity in relation to the negative control. The extracts from the stem were observed to have better antibacterial activity compared to the leaves. Gentamicin was used as a positive control. The ethanolic extracts had MIC range between 10mg/ml-&gt;10mg/ml while that of the methanolic extracts was &gt;10mg/ml. The results from this study validate the possible use of Eucalyptus torelliana in the production of new antimicrobial agents.

The phytochemical constituents and antibacterial activity of methanolic and ethanolic leaf and stem extracts of Eucalyptus torelliana, Nigeria

The phytochemical constituents and antibacterial activity of methanolic and ethanolic leaf and stem extracts of Eucalyptus torelliana, Nigeria

Comparative in-vivo Study of Laxative, Anti-diarrheoal and Anti-emetic activity of Ethanolic and Ethyl Acetate Leaf Extract of Citrus limon in Rats and Chicks

The aim of present work is to evaluate the laxative, anti-diarrhoeal and anti-emetic activity extract of Citrus limon by using different solvents like ethanol and ethyl-acetate. The methods employed are measuring fecal output in naive rats, loperamide induced constipation in rats, castor oil induced diarrhoea in rats and anti-emetic activity was evaluated using chick model. In fecal output in naive rats, the consistency of stools expelled within 24hrs after administration of extracts was observed. The loperamide induced constipation in rats using standard drug sodium picosulphate (5mg/kg), the faeces were collected after 8hrs and stool consistency was observed. At higher dose there is increase in fecal matter and compared with standard. The castor oil induced diarrhoea decreased total no. of feces was observed after 4hrs and these values are compared with control. In anti-emetic activity, the chicks after 10 minutes of copper sulphate pentahydrate(50mg/kg) administration there was an increase in no. of retchings (An emetic action without emeting gastric material) and the results are comparable with that of standard Chlorpromazine(150mg/kg).