Emerging Foodborne Pathogen Research Articles

The Significance of Mesophilic Aeromonas spp. in Minimally Processed Ready-to-Eat Seafood.

Minimally processed and ready-to-eat (RTE) seafood products are gaining popularity because of their availability in retail stores and the consumers’ perception of convenience. Products that are subjected to mild processing and products that do not require additional heating prior to consumption are eaten by an increasing proportion of the population, including people that are more susceptible to foodborne disease. Worldwide, seafood is an important source of foodborne outbreaks, but the exact burden is not known. The increased interest in seafood products for raw consumption introduces new food safety issues that must be addressed by all actors in the food chain. Bacteria belonging to genus Aeromonas are ubiquitous in marine environments, and Aeromonas spp. has held the title “emerging foodborne pathogen” for more than a decade. Given its high prevalence in seafood and in vegetables included in many RTE seafood meals, the significance of Aeromonas as a potential foodborne pathogen and a food spoilage organism increases. Some Aeromonas spp. can grow relatively uninhibited in food during refrigeration under a broad range of pH and NaCl concentrations, and in various packaging atmospheres. Strains of several Aeromonas species have shown spoilage potential by the production of spoilage associated metabolites in various seafood products, but the knowledge on spoilage in cold water fish species is scarce. The question about the significance of Aeromonas spp. in RTE seafood products is challenged by the limited knowledge on how to identify the truly virulent strains. The limited information on clinically relevant strains is partly due to few registered outbreaks, and to the disputed role as a true foodborne pathogen. However, it is likely that illness caused by Aeromonas might go on undetected due to unreported cases and a lack of adequate identification schemes. A rather confusing taxonomy and inadequate biochemical tests for species identification has led to a biased focus towards some Aeromonas species. Over the last ten years, several housekeeping genes has replaced the 16S rRNA gene as suitable genetic markers for phylogenetic analysis. The result is a more clear and robust taxonomy and updated knowledge on the currently circulating environmental strains. Nevertheless, more knowledge on which factors that contribute to virulence and how to control the potential pathogenic strains of Aeromonas in perishable RTE seafood products are needed.

Method to Study the Survival Abilities of Foodborne Bacterial Pathogens Under Food Processing Conditions.

The proper use of controllable atmospheric containers can facilitate investigations related to the survival abilities, and physiological states of key and emerging foodborne pathogens under recreated applicable food processing environmental conditions. Of particular note, the use of saturated salt solutions can efficiently control relative humidity in airtight containers. This chapter describes a practical experimental setup, with necessary prerequisites for exposing foodborne pathogens to simulated and relevant food processing environmental conditions. Subsequent analyses for studying cell physiology will also be suggested.

Current situation and future trends for beef production in the United States of America - A review.

USA beef production is characterized by a diversity of climates, environmental conditions, animal phenotypes, management systems, and a multiplicity of nutritional inputs. The USA beef herd consists of more than 80 breeds of cattle and crosses thereof, and the industry is divided into distinct, but ofttimes overlapping sectors, including seedstock production, cow-calf production, stocker/backgrounding, and feedlot. Exception for male dairy calves, production is predominantly pastoral-based, with young stock spending relatively brief portions of their life in feedlots. The beef industry is very technology driven, utilizing reproductive management strategies, genetic improvement technologies, exogenous growth promoting compounds, vaccines, antibiotics, and feed processing strategies, focusing on improvements in efficiency and cost of production. Young steers and heifers are grain-based diets fed for an average of 5 months, mostly in feedlots of 1,000 head capacity or more, and typically are slaughtered at 15 to 28 months of age to produce tender, well-marbled beef. Per capita beef consumption is nearly 26 kg annually, over half of which is consumed in the form of ground products. Beef exports, which are increasingly important, consist primarily of high value cuts and variety meats, depending on destination. In recent years, adverse climatic conditions (i.e., draught), a shrinking agricultural workforce, emergence of food-borne pathogens, concerns over development of antimicrobial resistance, animal welfare/well-being, environmental impact, consumer perceptions of healthfulness of beef, consumer perceptions of food animal production practices, and alternative uses of traditional feed grains have become increasingly important with respect to their impact on both beef production and demand for beef products. Similarly, changing consumer demographics and globalization of beef markets have dictated changes in the types of products demanded by consumers of USA beef, both domestically and abroad. The industry is highly adaptive, however, and responds quickly to evolving economic signals.

Integration Host Factor Modulates the Expression and Function of T6SS2 in Vibrio fluvialis.

Vibrio fluvialis, an emerging foodborne pathogen of increasing public health concern, contains two distinct gene clusters encoding type VI secretion system (T6SS), the most newly discovered secretion pathway in Gram-negative bacteria. Previously we have shown that one of the two T6SS clusters, namely VflT6SS2, is active and associates with anti-bacterial activity. However, how its activity is regulated is not completely understood. Here, we report that the global regulator integration host factor (IHF) positively modulates the expression and thus the function of VflT6SS2 through co-regulating its major cluster and tssD2-tssI2 (also known as hcp-vgrG) orphan clusters. Specifically, reporter gene activity assay showed that IHF transactivates the major and orphan clusters of VflT6SS2, while deletion of either ihfA or ihfB, the genes encoding the IHF subunits, decreased their promoter activities and mRNA levels of tssB2, vasH, and tssM2 for the selected major cluster genes and tssD2 and tssI2 for the selected orphan cluster genes. Subsequently, the direct bindings of IHF to the promoter regions of the major and orphan clusters were confirmed by electrophoretic mobility shift assay (EMSA). Site-directed mutagenesis combined with reporter gene activity assay or EMSA pinpointed the exact binding sites of IHF in the major and orphan cluster promoters, with two sites in the major cluster promoter, consisting with its two observed shifted bands in EMSA. Functional studies showed that the expression and secretion of hemolysin-coregulated protein (Hcp) and the VflT6SS2-mediated antibacterial virulence were severely abrogated in the deletion mutants of ΔihfA and ΔihfB, but restored when their trans-complemented plasmids were introduced, suggesting that IHF mostly contributes to environmental survival of V. fluvialis by directly binding and modulating the transactivity and function of VflT6SS2.

EFFECTS OF TEMPERATURE ON BIOFILM FORMATION AND QUORUM SENSING OF AEROMONAS HYDROPHILA

Aeromonas hydrophila is an emerging foodborne pathogen that causes infections more frequently in summer than in winter. This study evaluated the effects of temperature (4-37°C) on the biofilm formation and quorum sensing abilities of A. hydrophila on microtiter plates, stainless steel (SS), and crab surfaces. The incubation of the bacterium in Luria-Bertani broth at temperatures of 20-25°C significantly (P < 0.05) enhanced the biofilm formation and intra-species quorum sensing ability (via C4-AHL and C6-AHL). Field-emission electron microscopy revealed that the bacterium colonized the surface of crab and formed biofilms at 25°C. Thus, the present study demonstrates that temperature control in food processing environments may reduce A. hydrophila biofilm formation. Therefore, the study has significant applications in food processing plants.

Are French pig farmers and veterinarians knowledgeable about emerging foodborne pathogens? The case of hepatitis E virus

Hepatitis E virus (HEV) is an emerging zoonotic pathogen mainly transmitted via food in developed countries, and for which domestic pigs are recognised as the main reservoir. To implement an efficient HEV surveillance and control plan in the pig production sector, it is first necessary to assess the level of knowledge of pig-farming main actors about this pathogen. To this aim, an online survey was conducted between September and October 2017 to evaluate pig farmers’ and veterinarians’ knowledge about HEV epidemiology and its zoonotic potential. The questionnaire was filled in by 383 pig farmers and 46 pig veterinarians. Of this population, 77.8% of farmers and 8.7% of veterinarians had never heard of HEV. Our results highlight knowledge gaps among responding farmers, especially regarding the clinical and epidemiological features of HEV, while veterinarians appear to be well-informed about this pathogen. These findings indicate significant room for further improvement and the need for more information aimed at French pig farmers, with veterinarians acting as a priority channel through which information may be transferred from scientists to farmers. These educational efforts will facilitate farmers’ involvement in future HEV surveillance and control plans.

Development of a rapid method for the detection of Yersinia enterocolitica serotype O:8 from food

In this study, a new and alternative method based on monoclonal antibodies (MAbs) for the rapid detection of Yersinia enterocolitica O:8 was developed. This microorganism is an emerging foodborne pathogen causing gastrointestinal disease in humans. The transmission can occur through contaminated food such as raw or undercooked meat, milk and dairy products, water and fresh vegetables. Nine MAbs (46F7, 54B11, 54C11, 62D10, 64C7, 64C10, 72E8, 72E10, 72G6) were characterized and selected versus Y. enterocolitica O:8, and only 2 of them showed also a weak cross-reaction with Campylobacter jejuni. The MAb 54B11 was used for the development of Y. enterocolitica capture-ELISA in food matrices, i.e. meat and dairy products (n = 132). The method was validated by ISO 16140:2003 and compared with the official method for the detection of presumptive pathogenic Y. enterocolitica (ISO 10273:2003). Relative accuracy, sensitivity and specificity corresponded to 100%. The selectivity was evaluated on other food samples (n = 126) showing a lower confidence limit of 90.3% and an upper confidence limit of 100%. The results from this study demonstrated that the developed method was rapid and cheap, specific and sensitive for the screening of the pathogen in food.

Comparative Isolation of &amp;lt;i&amp;gt;Escherichia coli&amp;lt;/i&amp;gt; 0157:H7 from Diarrhoeic and Non-Diarrhoeic Children in Selected Communities in Cross River State, Nigeria

<i>Escherichia coli</i> O157:H7 has been considered an emerging foodborne pathogen causing severe diarrheal disease globally especially among children under the age of five years in Sub-Saharan Africa. This study was aimed at conducting a comparative study on the rate of isolation of <i>Escherichia coli</i> O157:H7 from diarrhoeic and non- diarrhoeic children in selected communities in Cross River State, Nigeria. Stool samples were collected from children under the age of five yrs and the pathogen isolated and identified using standard microbiological and biochemical procedures. Serological analysis to detect E. coli 0157:H7 serotype was carried out using Enzyme-linked immunosorbent assay (ELISA) and anti-rabbit H7 latex serum agglutination techniques. Out of 367 diarrhoeic children sampled, 70 (19.07%) were positive for E. coli 0157:H7 and the prevalence differed significantly (<i>p</i><0.05) with the control. The prevalence of <i>E. coli</i> 0157:H7 between diarrhoeal (19.07%) and non-diarrhoeal (1.39%) cases also differed significantly (<i>p</i><0.05) among the sampling areas. Diarrhoeric children below the age of one year had significantly highest prevalence of 26.83% at p<0.05 though no significant relationship between the sex of the children and the rate of infection with the organism was observed. Children diarrhoaeic stool therefore serves as a major vehicle in the domestic transmission of <i>Escherichia coli</i> O157:H7. Improved personal hygiene and environmental sanitation among parents and care givers can reduce the spread of diarrheal disease caused by this pathogen amongst children under the age of five years.

Assessing viability and infectivity of foodborne and waterborne stages (cysts/oocysts) of Giardia duodenalis, Cryptosporidium spp., and Toxoplasma gondii: a review of methods.

Giardia duodenalis, Cryptosporidium spp. and Toxoplasma gondii are protozoan parasites that have been highlighted as emerging foodborne pathogens by the Food and Agriculture Organization of the United Nations and the World Health Organization. According to the European Food Safety Authority, 4786 foodborne and waterborne outbreaks were reported in Europe in 2016, of which 0.4% were attributed to parasites including Cryptosporidium, Giardia and Trichinella. Until 2016, no standardized methods were available to detect Giardia, Cryptosporidium and Toxoplasma (oo)cysts in food. Therefore, no regulation exists regarding these biohazards. Nevertheless, considering their low infective dose, ingestion of foodstuffs contaminated by low quantities of these three parasites can lead to human infection. To evaluate the risk of protozoan parasites in food, efforts must be made towards exposure assessment to estimate the contamination along the food chain, from raw products to consumers. This requires determining: (i) the occurrence of infective protozoan (oo)cysts in foods, and (ii) the efficacy of control measures to eliminate this contamination. In order to conduct such assessments, methods for identification of viable (i.e. live) and infective parasites are required. This review describes the methods currently available to evaluate infectivity and viability of G. duodenalis cysts, Cryptosporidium spp. and T. gondii oocysts, and their potential for application in exposure assessment to determine the presence of the infective protozoa and/or to characterize the efficacy of control measures. Advantages and limits of each method are highlighted and an analytical strategy is proposed to assess exposure to these protozoa.

Emerging Biological Risks in a Global Context: An Introduction.

Foodborne diseases are one of the most serious problems in public health and one of the main causes of illness and death. It is estimated that around 600 million cases of gastroenteritis occur worldwide each year. At present, more than 200 foodborne diseases are known, which can cause from mild gastroenteritis to syndromes with a fatal outcome, with the added possibility of chronic complications, and more than 40 agents have been described that can cause foodborne diseases. Some of the most relevant foodborne pathogens are neglected or their impact underestimated such as foodborne viruses and parasites. Most of the foodborne pathogenic microorganisms possess a zoonotic potential with high environmental impact by the intensive animal production, and consequently the environment plays a very important role in their transmission. Consequently, a coordinated approach must be implemented to control emerging foodborne pathogens in primary production (animal health), in the community (human health), and in the environment (environmental health).

Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran.

Aim:Shiga toxigenic Escherichia coli (STEC) strains as emerging groups of foodborne pathogens are responsible for most foodborne illnesses. The aim of this study was to determine the antibiotic resistance pattern in STEC isolated from traditional milk products and their molecular characterization.Materials and Methods:A total of 116 samples were randomly purchased from local markets in Kashan, Iran, and evaluated for the occurrence of STEC by culturing and molecular methods. The antibiotic resistance of obtained isolates was determined by Kirby Bauer method. Furthermore, isolates were assayed for the presence of Shiga toxins (stx1 and stx2) and intimin gene (eae).Results:The incidence of E. coli in 60 ice cream, 30 yoghurt, and 26 cheese samples was 8.33%, 10%, and 11.54%, respectively. The findings showed that 11 out of 11 (100%) E. coli had both stx1 and stx2 while eae gene was not found in E. coli isolated of traditional milk products. For E. coli strains carrying stx1 and stx2, highest antibiotic sensitive levels were related to trimethoprim/sulfamethoxazole, norfloxacin, chloramphenicol, and ciprofloxacin, respectively.Conclusion:The results showed relationship between the presence of virulence factors and antimicrobial resistance. These results can be used for further studies on STEC as an emerging foodborne pathogen.

MOLECULAR IDENTIFICATION AND ANTIBIOGRAM OF Enterococcus spp. ISOLATED ON ENTEROCOCCUS SELECTIVE DIFFERENTIAL (ESD) MEDIA FROM MEAT, MEAT PRODUCTS AND SEAFOOD IN LIBYA

This study was conducted to investigate the presence of Enterococcus spp. in meat, meat products and seafood. A hundred and four samples were randomly collected from different geographic localities in Libya. The samples were subjected to microbiological analysis for enumeration and isolation of Enterococcus spp. by conventional cultural and molecular identification using PCR and partial sequencing of 16S rDNA techniques. Out of 104 samples, 73 (70.2%) isolates were found to be enterococci based on their cultural characteristics on ESD medium. However, out of 36 samples subjected to molecular identification, only six isolates were confirmed to be Enterococcus spp. using PCR and partial sequencing of 16S rDNA technique. All enterococci strains tested for their antibiotic sensitivity profiles showed high percentage of multi-resistance phenotype. These results can be used for further studies on enterococci as an emerging food borne pathogen and its role in human infection in Libya and would suggest that meat, meat products and seafood might play a role in the spreading of enterococci through the food chain with antimicrobial resistance characteristics.

Virulence gene profiles of Arcobacter species isolated from animals, foods of animal origin, and humans in Andhra Pradesh, India.

Aim::This study aimed to detect putative virulence genes in Arcobacter species of animal and human origin.Materials and Methods::A total of 41 Arcobacter isolates (16 Arcobacter butzleri, 13 Arcobacter cryaerophilus, and 12 Arcobacter skirrowii) isolated from diverse sources such as fecal swabs of livestock (21), raw foods of animal origin (13), and human stool samples (7) were subjected to a set of six uniplex polymerase chain reaction assays targeting Arcobacter putative virulence genes (ciaB, pldA, tlyA, mviN, cadF, and cj1349).Results::All the six virulence genes were detected among all the 16 A. butzleri isolates. Among the 13 A. cryaerophilus isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 61.5, 84.6, 76.9, 76.9, 61.5, and 61.5% of isolates, respectively. Among the 12 A. skirrowii isolates, cadF, ciaB, cj1349, mviN, pldA, and tlyA genes were detected in 50.0, 91.6, 83.3, 66.6, 50, and 50% of isolates, respectively.Conclusion::Putative virulence genes were detected in majority of the Arcobacter isolates examined. The results signify the potential of Arcobacter species as an emerging foodborne pathogen.

Molecular Typing of Vibrio parahaemolyticus Strains Isolated from Mollusks in the North Adriatic Sea.

Vibrio parahaemolyticus is an emerging foodborne pathogen in the Mediterranean, usually associated with shellfish consumption. The increase in the number of outbreaks in Europe is primarily associated with the global warming of the ocean that has a great impact on the spread and genetic selection of waterborne pathogens. The primary role of Italy in Europe's mollusk production, together with the fact that cases of infections with V. parahaemolyticus are not always notified to the European community, highlighted the necessity of acquiring new information about the epidemiological involvement of shellfish products. The aim of the study was to provide useful insights into the first steps of the Risk Assessment associated with V. parahaemolyticus through the molecular characterization of isolates from commercialized mollusks. A total of 102 strains identified as V. parahaemolyticus were investigated as part of a larger sampling (1-year survey) from several shellfish species collected from the Venice lagoon and the North Adriatic sea. All strains were characterized by multilocus sequence typing and tested for the presence of virulence genes (trh and tdh). The study of sampling/environmental factors and epidemiological analyses was performed to describe the behaviors of the different genetic populations. The population structure analysis highlighted three genetic clusters that could be subject to temperature selection during cold (≤15°C) and warm (>16°C) seasons. Moreover, other factors, such as molluscan species (clams/mussels), probably played a role in the distribution of genetic clusters. Although few strains carried the virulence factors (n = 6 trh+), epidemiological links with clinical isolates and a local dissemination of some sequence types were underlined. This work provides a useful background on the genotype spread as a first step in the Hazard Identification in light of future climate changes.

Genetic Diversity of Arcobacter Species of Animal and Human Origin in Andhra Pradesh, India.

Arcobacter is an emerging foodborne pathogen having zoonotic significance. Enterobacterial repetitive intergenic consensus (ERIC) PCR and repetitive sequence-based PCR (rep-PCR) analysis of a total of 41 Arcobacter isolates revealed a greater degree of genetic diversity. ERIC-PCR genotyping distinguished 14, 13 and 12 genotypes among 16, 13 and 12 isolates of A. butzleri, A. cryaerophilus and A. skirrowii, respectively. Rep-PCR genotyping distinguished 15, 12 and 11 genotypes among 16, 13 and 12 isolates of A. butzleri, A. cryaerophilus and A. skirrowii, respectively. The discriminatory power for ERIC and rep-PCR was found to be 0.997 and 0.996, respectively. Close clustering between isolates of animal and human origin are indicative of probable zoonotic significance.

Food Safety and Climate Change: Seasonality and Emerging Food Borne Pathogens

The future consequences of climate change and its impact on food safety is still not clear due to many variations and uncertanities. It is obvious that seasonal distribution and incidence of campylobacteriosis, salmonellosis and vero-toxigenic Escherichia coli borne diseases are increasing as the environmental temperature increases. This review summarizes the studies and data acquired about the relationship of climate change and emerging food borne diseases. Globalisation triggered a change in socio-economical life style which affects touristic activities, food consumption, agricultural tendecies and changing demands. Additionally, changes in global trade including fair trade cause a globalization of local kitchens.

Vero-Toxigenic Escherichia Coli (VTEC): To Sum up all we know

Verotoxigenic Escherichia coli renamed after detection of production ability of cytotoxin which kills Vero cells, a continuous cell line derived from the kidney of the African green monkey (Chlorocebus sp). These toxins are proved to cause severe diseases in humans. As an emerging food borne pathogen group, VTEC infection may lead to life threatening haemolytic-uremic syndrome (HUS).

Antibacterial Activities of Ankaferd Hemostat (ABS) on Shiga Toxin-Producing Escherichia coli and Other Pathogens Significant in Foodborne Diseases.

Objective:Ankaferd hemostat (Ankaferd Blood Stopper®, ABS)-induced pharmacological modulation of essential erythroid proteins can cause vital erythroid aggregation via acting on fibrinogen gamma. Topical endoscopic ABS application is effective in the controlling of gastrointestinal (GI) system hemorrhages and/or infected GI wounds. Escherichia coli O157:H7, the predominant serotype of enterohemorrhagic E. coli, is a cause of both outbreaks and sporadic cases of hemorrhagic colitis. The aim of this study is to examine the effects of ABS on 6 different Shiga toxigenic E. coli serotypes including O26, O103, O104, O111, O145, and O157 and on other pathogens significant in foodborne diseases, such as Salmonella Typhimurium, Campylobacter jejuni, and Listeria monocytogenes, were also assessed.Materials and Methods:All strains were applied with different amounts of ABS and antimicrobial effect was screened. S. Typhimurium groups were screened for survival using the fluorescence in situ hybridization technique.Results:The relative efficacy of ABS solutions to achieve significant logarithmic reduction in foodborne pathogens E. coli O157:H7 and non-O157 serogroups and other emerging foodborne pathogens is demonstrated in this study. ABS has antibacterial effects.Conclusion:Our present study indicated for the first time that ABS may act against E. coli O157:H7, which is a cause of thrombotic thrombocytopenic purpura, hemolytic-uremic syndrome, and hemorrhagic colitis. The interrelationships between colitis, infection, and hemostasis within the context of ABS application should be further investigated in future studies.

Molecular Epidemiology and Strain Comparison between Hepatitis E Viruses in Human Sera and Pig Livers during 2014 to 2016 in Hong Kong.

Hepatitis E virus (HEV) causes substantial morbidity and mortality in developing countries and is considered an emerging foodborne pathogen in developed countries in which it was previously not endemic. To investigate genetic association between human HEV infection and HEV-contaminated high-risk food in Hong Kong, we compared local virus strains obtained from hepatitis E patient sera with those surveyed from high-risk food items during 2014 to 2016. Twenty-four cases of laboratory-confirmed human HEV infections were identified from January 2014 to March 2016 in our hospitals. Five types of food items at risk of HEV contamination were purchased on a biweekly basis from April 2014 to March 2016 in two local market settings: supermarkets (lamb, oyster, and pig liver) and wet markets (oyster, pig blood curd, pig large intestine, and pig liver). HEV RNA detection was performed by a real-time reverse transcription-PCR assay. HEV RNA was detected in pig liver, pig intestine, and oyster samples with prevalences of 1.5%, 0.4%, and 0.2%, respectively. Neighbor-joining phylogenetic inference showed that all human and swine HEV strains belonged to genotype 4. HEV subtype distributions in humans and swine were highly comparable: subtype 4b predominated, while subtype 4d was the minority. Local human and swine HEV genotype 4 strains shared over 95% nucleotide identity and were genetically very similar, implicating swine as an important foodborne source of autochthonous human HEV infections in Hong Kong. Action should be taken to raise the awareness among public and health care professionals of hepatitis E as an emerging foodborne disease.

Campylobacter jejuni: An Emerging Foodborne Pathogen of Global Significance

Emerging foodborne pathogens are significant causes of morbidity and mortality both in developed as well as developing nations. Campylobacter jejuni is an important infectious emerging foodborne bacterial zoonotic agent that causes gastrointestinal illness in humans. The infection can occur in sporadic and epidemic form. The post infections sequelae with C. jejuni are Guillain-Barre syndrome, reactive arthritis and irritable bowel syndrome. Globally, about one-third of Guillian-Barre syndrome cases are attributed to Campylobacter infections. It is estimated that 2.5 million cases of campylobacteriosis occur each year in the United States. The annual economic cost due to Camyplobacter associated illnesses is USA reaches up US $ 8 billion. The source of infection is exogenous, and oral-fecal route is the principal mode of transmission of Campylobacter jejuni. The infection occurs through consumption of contaminated raw or undercooked meat (poultry, beef, pork, and lamb), unpasteurized milk, and untreated water. The cross-contamination from raw meat, and direct contact with infected or reservoir hosts can also be source of infection. Poultry is responsible for 50%-70% of human Campylobacter infections. Laboratory help is required to make an unequivocal diagnosis of disease. Most cases of Campylobacter jejuni are self-limited and generally do not require treatment. However, antimicrobial therapy with azithromycin, clarithromycin, erythromycin, chloramphanicol and gentamicin are indicated in severe cases. Certain measures such as consumption of pasteurized milk, cooked red meat and chicken, chlorinated water, application of hazard analysis critical control point in food sector, bio-security at poultry farm, sanitation in abattoir, checking cross contamination, surveillance, and health education will certainly reduce the incidence of this emerging foodborne disease. Further work on the molecular epidemiology, pathogenesis, and chemotherapy camplobacteriosis should be undertaken.