Elements Of Yield Structure Research Articles

YIELD STRUCTURE AND GRAIN QUALITY OF SPRING WHEAT VARIETIES OF ALTAI AND FOREIGN SELECTION (TYUMENTSEVSKY DISTRICT, ALTAI KRAI)

Wheat is one of the three major agricultural crops worldwide. The yield of any agricultural crop, including spring soft wheat, largely depends on compliance with regional requirements during its cultivation, while variety is one of the decisive factors. The current research aims to identify the difference in the yield structure and grain quality of spring wheat varieties of Altai and foreign selection for the Tyumentsevsky district (Altai Krai) for the first time.
 The tasks are as follows: (1) to analyze the growing season weather conditions, (2) to compare the yield, yield structure elements, and indicators of grain quality, and (3) to find the best variety of spring soft wheat. Sampling during the experiment and processing of the results was carried out in accordance with standard methods. There was a difference in the yield structure and grain quality of spring wheat varieties of Altai and foreign selection in the Tyumentsevsky district of Altai Krai. The 2020 growing season was extreme, with droughts, higher temperatures, and precipitation lower than in multiannual terms.
 A comparison of varieties has shown the highest yield variability. The highest amount of grains (21.1 c/ha) was received by Variant 2 – Altayskaya 75 (elite seeds). The research results have shown that it is best to use the varieties of local selection to obtain the maximum yield of spring soft wheat in the conditions of the Tyumentsevsky district of Altai Krai.

DIFFERENCES IN COMMON BEAN VARIETIES WITH RESPECT TO VALUABLE ECONOMIC TRAITS

As a result of this research, a number of variety varieties have been identified, which are distinguished by a set of valuable economic traits. The differences of variety samples were established by a comparative evaluation of the manifestation of traits in comparison with the standard. Among the bean varieties under study, 25 pieces were classified as early-ripening (27.8%). (27.8%), medium-early - 36.0 pcs. (40.0%), medium-ripening - 18.0 pcs. - (20%) and medium-early - 11 pcs. (12,2%). Common bean varieties with high attachment of the lower beans on the plant were identified. Among the totality of the original material submitted varieties were: UD0302930 (Ukraine) - 16.7 cm, UD0302957 (Ukraine) - 16.5 cm, UD0301502 (Ukraine) - 16.0 cm, Local 2 (Ukraine) - 15.6 cm. Compared with the standard, only cultivars UD0302930 (Ukraine) and UD0302957 (Ukraine) significantly exceeded the standard in terms of the height of lower bean attachment. The number of seeds per plant is a derived trait of the number of beans, so the varieties with the highest number of beans were also characterized by higher rates of seed number per plant. Consequently, UD0300411 (Romania) with 45.7 and UD0301736 with 44.2 seeds per plant were noted to have significantly exceeded the standard in this indicator. At level with the standard number of seeds per plant was found in samples UD0301043 (Turkey), Local 1 (Ukraine) - 38.9 pcs. The quantitative expression of the trait 1000 grains weight is a confirmation of the previously reported material, that in order to form the maximum expression of the trait in grain legume crops, including common bean, parental forms are included in hybridization for the expression of yield structure elements slightly above the average in the whole sample. That is, the varieties that stood out both in the number of beans per plant and the number of seeds per plant were inferior to other samples, in which the number of beans and the number of seeds per plant were lower. The highest mass of 1000 grains was characterized by the variety samples UD0300227 (USA) - 248.3 g, UD0300782 (Ukraine) - 221.2 g, Local 1 (Ukraine) - 224.3 g, UD0303753 ) - 214.3 g, UD0301736 - 208.3 g. In terms of yield as well as grain productivity, the following varieties stood out: UD0300227 (USA) - 402 g, UD0301736 - 405 g, UD0300411 (Romania) - 391 g, Local 1 (Ukraine) - 3 (4) g, which exceeded the standard at a significant level.

The effect of weather conditions on the spring barley variety ‘Yaromir’ and its productivity in the Ryazan region

The current study was carried out in 2017–2021 in the Competitive Variety Testing on the basis of the ISPA of the FSBSI “FRAC VIM”. The soil of the experimental plot was dark gray forest, heavy loamy in granulometric composition, of medium fertility. The hydrothermal conditions of the vegetation periods differed significantly in terms of the mean daily air temperature and the sum of effective temperatures, and the amount of precipitation. The years of 2018 and 2021 were characterized with very dry conditions with HThC of 0.51–0.64. The years of 2017, 2019 were arid with HThC of 0.72–0.85. The years of 2020 was humid, with a moisture coefficient of 1.34. The purpose of the current study was to establish the effect of weather conditions of the Ryazan region on the interstage periods, the yield structure and productivity of the spring barley variety ‘Yaromir’. There has been found a correlation between the length of vegetation period with the amount of precipitation and HThC in the interstage periods of ‘germination-tillering’ (r = +0.571 and r = +0.607, respectively), ‘tillering-coming into ear’ (r = +0.735 and r = +0.655, respectively) and ‘filling-ripening’ (r = +0.881 and r = +0.967, respectively). Strong positive correlations were found between ‘amount of precipitation’ and the yield structure elements of the variety, namely ‘number of productive stems per 1 m2 ’ (r = +0.969), ‘productive tilling capacity’ (r = +0.947), ‘plant height’ (r = +0.827). Mean correlations were found between ‘amount of precipitation’ and ‘length of head’ (r = +0.562) and ‘number of kernels per head’ (r = +0.453). Negative mean correlations were found between ‘amount of precipitation’ and ‘1000-kernel weight’ (r = -0.654). There has been established that the average daily air temperature of the vegetation period had no effect on the formation of the yield structure elements of this variety. There have been identified average correlations between ‘sum of effective temperatures’ and ‘productive tillering’ (r = +0.352) and ‘length of head’ (r = +0.538). Weather conditions did not have a significant effect on grain productivity of the variety. There has been noted a slight effect of precipitation and HThC in the period ‘tillering-coming into ear’. The minimum variation in the productivity over the years (Cv = 3.5%) and revealing 95.1% of productivity potential indicates that this variety is characterized by stable productivity and adaptability to the weather conditions of this region.

The Yield and Weed Infestation of Winter Oilseed Rape (Brassica napus L. ssp. oleifera Metzg) in Two Tillage Systems

Results from a four-year field experiment were used to evaluate the effect of conventional tillage (CT) and no-tillage (NT) systems on the yield, selected yield and crop structure elements, weed infestation, and contents of fat and glucosinolates in the seeds of winter oilseed rape (Brassica napus L. ssp. oleifera Metzg). The study proved the beneficial effect of the NT system on the winter rape seed yield only during the precipitation shortage in the growing season. In the years with a sufficient sum of precipitation, a higher seed yield was produced in the CT than in the NT system. Considering the average values from the four-year study period, the seed yield and straw yield, plant density after emergence and before harvest, number of branches and main shoot length, and finally the 1000 seed weight were significantly higher in the CT compared with the NT system. In contrast, the NT system proved more beneficial regarding seed weight per silique and fat content of the seeds. The statistical analysis of the study results showed no effect of the tillage systems on the glucosinolate content of the seeds. In turn, a significantly higher number and air-dry weight of weeds as well as an increased density of Viola arvensis weeds were demonstrated in the NT plot compared with the CT plot of winter oilseed rape. Sonchus asper and Sonchus arvensis were identified in the NT plot but not in the CT plot. A significantly higher density of Chenopodium album and Euphorbia helioscopia weeds was detected in the CT system compared with the NT system. The statistical analysis of study results regarding weed community diversity showed similar values to the Shannon-Weinner diversity index (H’) in both tillage systems and a significantly higher value of the Simpson dominance index (SI) in the NT system compared with the CT system.

Estimation of the yield structure elements of the collection winter durum wheat samples in the south of the Rostov region

Introduction and methodology. The value of the initial material is primarily determined by the tasks of modern breeding process, collection material playing an important role in it. The purpose of the current study was to estimate the initial material of winter durum wheat according to their yield structure elements in the south of the Rostov region. The objects of research were 159 winter durum wheat samples among which there were 69 of domestic breeding and 90 of foreign one.Results. The study has shown that in 2019–2020 grain productivity of the collection samples ranged from 227.9 g/m2 to 735.9 g/m2. All studied samples were divided into three groups according to the trait ‘productive stand’ (number of productive stems per 1 m2). There were 17.6% of samples with a small value of the trait (201–400 pcs./m2); with a mean value of the trait (401–600 pcs./m2) there were 72.3% of samples and with a large value of the trait (601–800 pcs./m2) there were 10.1% of samples. According to the trait ‘plant height’ more than a half of the samples (57.0%) belonged to the ‘semi-dwarf’ group (61–85 cm). Large part (70%) of winter durum wheat samples formed ‘length of head’ in the range of 6.5–7.5 cm. In 2019–2020, number of spikelets per head ranged from 17 pcs. up to 24 pcs. The trait ‘kernel weight per head’ of the studied collection samples varied from 0.77 g to 2.08 g, all the samples had different kernel sizes. The trait ‘number of kernels per head’ varied from 22 pcs. (the variety ‘Novinka 4’) up to 51 pcs. (the sample ‘1015/16’). The trait ‘1000-kernel weight’ in the trial ranged from 23.9 g to 49.0 g, the standard variety had 34.1 g. The productivity of the collection samples was formed due to 1000-kernel weight and kernel weight per head, the correlation coefficients for these productivity elements being r = 0.45 ± 0.07; r = 0.35 ± 0.07.

The Influence of Different Nitrogen Fertilizer Rates, Urease Inhibitors and Biological Preparations on Maize Grain Yield and Yield Structure Elements

The field experiment was performed in 2019–2021 at the Experimental Station of Vytautas Magnus University Agriculture Academy (54°52′ N, 23°49′ E). The soil of the experimental field was Endohipogleyic-Eutric Planasol. The studied factors were: Factor A—different nitrogen fertilizer rates: (1) 100 kg N ha−1; (2) 140 kg N ha−1; (3) 180 kg N ha−1; Factor B—the use of urease inhibitors (UI) and biological preparations (BP): (1) urease inhibitors (UI) and biological preparations (BP) were not used; (2) Urease inhibitor (UI ATS)—ammonium thiosulfate—[(NH4)2S2O3 12-0-0-26S]; (3) Urease inhibitor (UI URN)—N-butyl-thiophosphorus triamide (NBPT) and N-propyl-thiophosphorus triamide (NPPT); (4) Biological preparation (BP HUM)—suspension of humic and fulvic acids; (5) Biological preparation (BP FIT)—Ascophyllum nodosum suspension. Our studies showed that the highest yield of maize grain (8.9–12.0 t ha−1) was obtained by fertilizing with N180 and using the urease inhibitor ammonium thiosulfate (ATS). ATS significantly increased corn grain yield in all backgrounds of nitrogen fertilization. The investigated urease inhibitors and biologics had a higher and more significant (p < 0.05) effect on maize grain yield when fertilized with N100 nitrogen. The increase in nitrogen fertilizer rates had an effect on maize grain yield, with the largest increase in yield being found in the increase in nitrogen rate from N100 to N140, and the increase in rate to N180 was less effective. The maximum mass of 1000 grains (323.5 g) was determined in 2019 by fertilization with N180 and use of the urease inhibitor UI URN. The urease inhibitor UI ATS was more effective when fertilized with lower rates of N100 and N140. Positive, moderate, strong and very strong, statistically significant correlations (r2 = 0.48–0.91) were most often found between the latter indicators and nitrogen fertilizer rates throughout the study year. The largest amount of grain (497 units) in the cob was determined in 2019, using fertilization with N140 and UI ATS, but no significant differences were found between the different fertilizer rates and the tested preparations. These results suggest that urease inhibitors and biologics can reduce dependence on nitrogen fertilizers and increase maize yield, a technology that should be practiced by maize growers.

Influence of seed pre-sowing treatment with growth activating preparations on the durum spring wheat productivity

To provide pasta production with native high-quality raw materials, it is necessary not only to expand the sown area of durum wheat, but also to increase its actual yield. In conditions of environmental crisis, the technology elements aimed at activating metabolic processes in a plant organism become more and more relevant, which makes it possible to significantly increase productivity while saving resources. Durum spring wheat is characterized by weak rooting and slow accumulation of vegetative mass, and is also sensitive to the lack of microelements in the soil. Therefore, it is important to study the effectiveness of complex preparations, which, in addition to growth regulators, contain micro- and macro-elements, and other substances necessary to stimulate growth processes, especially in conditions of moisture deficiency. The effectiveness of multicomponent growth activating preparations was studied on various agricultural crops (winter wheat, barley, sunflower, mustard, etc.). At the same time, the effect of durum spring wheat pre-sowing treatment under insufficient moisture conditions has not been studied enough. The purpose of the research. To study the effect of seed treatment with growth activating preparations with a different active substance composition on the durum spring wheat yield under insufficient moisture conditions. Research methodology. The studies were carried out on the experimental field of the Kharkiv NAU named after V. V. Dokuchayev in 2018–2020. Seed treatment with Vympel, Regoplant, Vermistim and Gulliver preparations was carried out before sowing by the method of semi-dry dressing. Registration plot area was 10 m2. Research results and their discussion. It was found that pre-sowing growth stimulation by activating preparations Vympel and Vermistim promotes an increase in seed germination energy by 4–5 %, laboratory germination – by 2–3 %, field germination – by 5 % in comparing to control. Seed stimulation also had a positive effect on pre-harvest planting density and ear productivity. In case with Vympel, the number of productive ears per 1 m2 exceeded the control by 10 %, in case with Vermistim – by 12 %. At the same time, the mass of grain in an ear increased by 21,3 and 26,2 %, respectively. As a result of changes in the plant productivity elements, wheat productivity increased with the use of Pennant by 16 %, Vermistim – by 14 % of the control. In dry years, the effectiveness of these preparations was higher. A positive effect was also established when using the Gulliver and Regoplant for seed treatment, however, it was insignificant (3 and 4 %). Conclusions. The positive effect of pre-sowing seed treatment with multicomponent growth activating drugs Vympel and Vermistim on germination and the formation of the yield structure elements contributed to an increase in durum spring wheat grain productivity by 16 % and 14 % from control. The positive effect of the using these drugs increased under more stressful conditions of wheat growing. The effectiveness of the using Gulliver and Regoplant was insignificant and unstable over the years. Key words: durum spring wheat, seed treatment, growth activating preparations, productivity elements, yield.

The study results of the promising pea lines in the soil and climatic conditions of the Kirov region

The current paper has presented the results of estimating pea varieties in the Competitive Variety Testing. The purpose of the study was to analyze pea lines at the final stage of the breeding process and to identify the promising ones according to economically valuable traits. The study of variety samples was carried out in laboratory for legumes breeding and primary seed production at the Falyonsky breeding station in 2018–2020. The objects of the study were 28 peas lines and varieties of various morphotypes. The estimation of samples, observations and accounting were carried out according to the methodology of the State Commission for Variety Testing. The standard varieties were the varieties ‘Krasnoufimsky 93’, ‘Ryabchik’, ‘Falyonsky usatyi’, zoned in the Volga-Vyatka region. The vegetation periods of 2018–2020 contrastingly differed among themselves in moisture supply and temperature regime, the most favorable hydrothermal conditions were in 2019, the most unfavorable ones were in 2018 and 2020. As a result of the study, there have been identified the lines that exceeded the standard varieties in productivity of grain, green mass and dry matter. In the group of white-flowered grain crops, there was identified the line ‘E-483’ with 3.49 t/ha of mean grain productivity, 31.4 t/ha of green mass and 5.28 t/ha of hay. In the group of color-flowered grain crops, the maximum productivity of grain, green mass and dry matter was identified in the line ‘E-3588’ with 4.16, 34.9 and 7.73 t/ha, respectively. Among the leafless variety samples, the line ‘E-1250’ produced the highest grain yield with 3.71 t ha. There have been identified the lines with high indicators of yield structure elements. The maximum number of fertile nodes was identified in the line ‘E-3745’ (4.2 pcs.). The maximum number of kernels was identified in the lines ‘E-483’, ‘E-3542’, ‘E-3798’ (5.8 pcs.). The maximum number of kernels per plant was identified in the line ‘D-24746’ (25.9 pcs.). The maximum seed productivity was identified in the line ‘E-3845’ (4.4 g). The highest values of the number of productive nodes and kernels were obtained in the group of white-flowered leafy varieties. The largest number of kernels per plant was identified in the maple pea. Seed productivity and 1000-kernel weight were higher in the leafless variety samples. According to stem length and resistance to lodging, there has been identified the advantage of the leafless lines.

ТЕХНІЧНА ЕФЕКТИВНІСТЬ БАКОВИХ СУМІШЕЙ ГЕРБІЦИДІВ У ПОСІВАХ СОНЯШНИКУ В УМОВАХ ПІВНІЧНОГО СТЕПУ УКРАЇНИ

At the current weed infestation level of chernozem, it is almost impossible to grow sunflowers without the regulated use of the most effective herbicides with different action spectrum on weeds. It was established that tank mixtures of ethametsulfuron-methyl herbicides (750 g/kg) – 25 g/ha + aclonifen (600 g/l) – 1.2 l/ha + surfactant Trend 90 – 300 ml/ha and ethametsulfuron-methyl (750 g/kg) – 25 g/ha + aclonifen (600 g/l) – 1.5 l/ha + surfactant Trend 90 – 300 ml/ha provided the highest technical efficiency and the lowest air-dry mass of weeds in the oilseed agrocenosis. Their technical efficiency was 61.2–65.8%. Almost all variants of herbicide tank mixtures showed a high phytotoxic effect on green amaranth (Amarantus retroflexus L.) – 51-61%, common ragweed (Ambrosia artemisiifolia) – 52–76%, field bindweed (Convolvulus arvensis) – 100%, yellow foxtail (Setaria pumila L.) was the less controlled – 21– 31%. The variants with the application of herbicide tank mixtures of ethametsulfuron-methyl (750 g/kg) – 25 g/ha + aclonifen (600 g/l) – 1.2 l/ha + surfactant Trend 90 – 300 ml/ha showed the highest indicators of the yield structure elements, here the 1000 seed weight was 73.1 g at a maximum basket diameter of 31.5 cm. These indicators were less in 1.2–1.7 times for other studied variants with the herbicide tank mixtures. When application of the tank mixtures of ethametsulfuron-methyl (750 g/kg) – 25 g/ha + aclonifen (600 g/l) – 1.2 l/ha + surfactant Trend 90 – 300 ml / ha and ethametsulfuron -methyl (750 g/kg) – 25 g/ha + aclonifen (600 g/l) – 1.5 l/ha + surfactant Trend 90 – 300 ml/ha, it was obtained the maximum yield of sunflower seeds 2.15 and 2.07 t/ha, respectively. Other variants with herbicides and their tank mixtures had significantly lower yield of sunflower seeds – by 23–27 %. Key words: sunflower, weeds, herbicides, tank mixes, yield, technical efficiency

ELEMENTS OF YIELD STRUCTURE OF MAIZE HYBRIDS DEPENDING ON THE APPLICATION OF BIOLOGICAL PREPARATIONS IN FOREST-STEPPE CONDITIONS

The article presents the results of research on the effect of various biological preparations on productivity and formation of elements of grain yield structure of mid-season hybrid maize DKS 4014. A promising direction to increase plant productivity of maize and realization of its biological potential is the ecologization of cultivation technology of crops, creation of effective, environmentally safe biological preparations and development of technologies of their application is one of the priority directions in the scientific support of the agroindustrial complex of Ukraine. The use of such preparations is possible, both independently and in combination with other components of cultivation technology, such as mineral fertilizers and plant protection products. The use of biological preparations restores soil structure, restores fertility and accelerates the mineralisation processes in the soil. The effect of soil application of biofertilizer based on active strains of phosphorus-mobilizing and nitrogen-fixing bacteria and physiologically active substances, both alone and in combination with mycorrhizobearing bio-preparation treatment of maize seeds for active settling of root and root zone of maize hybrids was studied. The results of the obtained results of the study of biometric indicators, grain yield of maize depending on the application of soil biofertilizer, mycorrhizoforming biological preparation, and the combination of these preparations, as well as the application of soil herbicide together with a carrier-adhesive showed the improvement and increase in biometric indicators and productivity of maize, so we recommend the use of these preparations in the conditions of production. The best indicators of improving the structural elements of maize yield and yield in general were provided by the complex of preparations Graundfiks 4 l/ha + Micofrend 6 kg/ha, where the yield of mid-season hybrid DKS 4014 was 13.65 t/ha, which is 29.9% more than the control.

The effect of biological products and microelement fertilizer ‘Organomix’ on maize productivity in the southern part of the Rostov region

The current paper has presented the study results on the effect of biological products and microelement fertilizers ‘Organomix’ on productivity of the maize hybrid ‘Zernogradsky 354MV’ carried out in laboratory for cultivation technologies of row crops (FSBSI “ARC “Donskoy”) in 2019–2020. The soil in the experimental plot was favorable for the cultivation of corn, containing 3.36% of humus in the arable layer, 24.4 mg of mobile phosphorus, and 360 mg of exchangeable potassium per 1 kg of soil. The soil pH was 7.0. The study was carried out to estimate the effect of the use of biological products for seed treatment and microelement fertilizers ‘Organomix’ for plant treatment on productivity and economic efficiency of maize cultivation. There was low moisture content of sowings during the period of the trial. There was established an uneven distribution of precipitation, the value of the hydrothermal coefficient was less than 1 (0.64 in 2019 and 0.65 in 2020), which indicated the dryness of the vegetation period. The studied biological products and microelement fertilizers influenced the yield structure elements. The applied biological products and microelement fertilizers ‘Organomix’ improved survival rate of plants before harvesting (the plant density was 4.39–4.54 pcs/m2). There was increase of grain productivity indicators, namely cob weight ranged from 112.9 to 125.7 g, grain weight per ear varied from 94.4 to 104.8 g and 1000-grain weight was 221.2–231.4 g. The improvement of the yield structure elements increased grain productivity on 0.25–0.77 t/ha. Economic efficiency showed that the use of biological products and microelement fertilizers raised the conditional net income to the level of 28 061–34 821 rubles/ha, profitability up to 167.6–201.8% and reduced production costs to 4640–5231 rubles/t.

Cultivation technology elements influence on the harvest structure and quality of crops products

Topicality and methodology. The purpose of our research was to study the influence of the type of crop rotation, methods of basic land cultivation and fertilizers on the elements of winter wheat yield structure, spring barley and sugar beet and their qualitative characteristics. The studies were carried out on typical medium-thick low-humus heavy loamy chernozem on loess-like soil in the conditions of the Belgorod region. In The maximum increase in the indicators of crop yield structure was recorded on the organo-mineral fertilization system. With the cultivation of barley and winter wheat in a crop rotation, the quality of grain increases. When growing beets in crop rotation, there is a tendency to a decrease in sugar content in comparison with grain-row crop rotation, while the sugar yield increases. The organo-mineral fertilization system reduces the starch content in the grain of barley by an average of 2.4% in the crop rotation and 1.3% in the grain crop rotation compared to the control, and increases the protein content in the grain of winter wheat by an average of 1.4%. The sugar content of beets on the organo- mineral fertilization system decreases on average by 0.8%, while the sugar yield, on the contrary, increases. Land cultivation methods in most cases do not affect the technological qualities of crops.

The new middle-maturing winter barley variety ‘Marusya’

Last years the Rostov region has faced a significant climate continentality increase during the growing season of winter crops. This factor makes necessary to develop new varieties of intensive winter barley with a high level of ecological adaptability, resistance to lodging and leaf diseases, with a high level of potential productivity. The purpose of the current study was to develop a new highly productive, stress-resistant winter barley variety with a high level of adaptability and to evaluate it according to economically valuable traits. The current paper has presented the agrobiological characteristics of the winter barley variety ‘Marusya’, the originator and patent holder of which is the Federal State Budgetary Scientific Institution “Agrarian Research Center “Donskoy”. The research was carried out from 2018 to 2020 in the fields of experimental crop rotation of the department of barley breeding and seed production of the FSBSI “ARC “Donskoy”. The forecrop was peas. The accounting plot area was 10 m², the number of repetitions was 6. The winter barley variety ‘Timofey’ was used as a standard variety. The species was parallelum. The type of development was facultative. Over the years of study in the Competitive Variety Testing of the FSBSI “ARC “Donskoy” (2018–2020), the variety produced from 8.0 to 11.2 t/ha, that exceeded the standard variety ‘Timofey’ on 0.5–1.5 t/ha. Since 2020, the State Commission for Variety Testing of Agricultural Crops decided to include the variety ‘Marusya’ in the State List of Breeding Achievements in the North Caucasus region. The variety is being studied in the Central Blackearth and Lower Volga regions of the Russian Federation. The consistently high productivity of the new variety is achieved due to the large grain and higher indicators of the yield structure elements and resistance to diseases in comparison with those of the standard variety ‘Timofey’

Water deficiency tolerance of genetically modified common wheat cv. Zymoyarka, containing a heterologous ornithine-δ-aminotransferase gene

Aim. To determine water deficiency tolerance of genetically modified common wheat plants (Triticum aestivum L., cv Zymoyarka), containing the heterologous ornithine-δ-aminotransferase gene, based on the analysis of grain productivity and physiological and biochemical characteristics in transgenic and non-transgenic genotypes. Methods. Biochemical spectrophotometric assays: the enzyme ornithine-δ-aminotransferase activity, the free L-proline content, and the photosynthetic pigments content; biotechnological: Agrobacterium-mediated transformation in planta; physiological: morphometric traits and elements of grain productivity; mathematical statistics. Results. It was established that the presence of an additional copy of the ornithine-δ-aminotransferase gene in transgenic plants leads to higher activity of the ornithine-δ- aminotransferase enzyme: by 1.6 times higher on average for all lines as compared to the non-transgenic plants at 70 % of fi eld capacity and by 1.5 – at 30 % fi eld capacity. However, transgenic plants did not differ significantly from the original variety in the free L-proline content either under optimal water conditions or under soil drought. The increase in the total chlorophyll (a + b) content in flag leaves of transgenic plants was established under conditions of both optimal water supply and drought, as compared with the original genotype (increase by 5–7 % and 8–11 %, respectively). The enhanced expression of the orni- thine-δ-aminotransferase gene in the transgenic plants stimulated root growth both under optimal and stressful conditions: the root length of the transformed plants exceeded that of the original variety by 3.4–3.9 cm in the variant with optimal water supply, and by 4.2–4.6 cm – under drought. They were also characterized by a more developed root system. Dry root weight of the transgenic plants exceeded the original variety both in the control (by 23–27 %), and under drought (by 37– 44 %). Under drought, the root dry weight decreased by 29 % in the plants of the original variety, compared 70 % fi eld capacity, and by 11–15 % in the lines. Under 30 % field capacity, the transgenic lines also exceeded non-transformed plants in the number of grains from the whole plant (on average for 3 lines by 26 %) and in the grain weight (by 22 %). Transgenic plants are characterized by the formation of a higher productive shoots number: from 3.2 to 3.4 compared with 2.5 in non-transgenic plants at 70 % fi eld capacity and 2.7–3.1 vs 2.2 at 30 % field capacity it was found. Conclusions. Thus, the analysis of genetically modified common wheat plants cv. Zymoyarka, containing the heterologous alfalfa ornithine-δ- aminotransferase gene, by yield structure elements, morphometric parameters and photosynthetic pigment content showed their better tolerance to soil drought as compared to non-transgenic plants. We explain the improvement of grain productivity of the whole plant in transgenic wheat lines with an additional copy of ornithine-δ-aminotransferase gene by the fact that they have a better developed root system (dry root weight of the transgenic plants exceeded the original variety both in the control by 23–27 %, and under drought by 37–44 %) and a higher (on average for 3 lines – 3.3 compared to 2.5 in non- transgenic plants at 70 % fi eld capacity and 2.9 vs 2.2 at 30 % fi eld capacity) number of productive shoots than in the origi- nal variety both under optimal and insuffi cient water supply.

Economic and energy estimation of the effect of seeding rates of spring peas in the south of the Rostov region

For Russia, peas are traditional legumes, used both in the food industry for its excellent taste, and in animal husbandry for the high content of feed units and balanced protein, which allows reducing the cost of animals’ feeding. The purpose of the current study was to estimate the effect of the seeding rates of peas on its productivity, feed value of its beans, economic and bioenergetic cultivation efficiency. The current paper has presented the study results obtained in the laboratory for cultivation technology of row crops (FSBSI “Agricultural Research Center “Donskoy”) in 2019–2020 where there was studied the effect of seeding rates on productivity of the middle maturing pea variety ‘Aksaysky Usatii 5’ of the Don breeding. The soil of the experimental plot was favorable for growing peas with 3.36% of humus in the arable, with 7.0 pH, 24.4 mg/kg of soil of P2O5, 360 mg/kg of soil of K2O. The studied seeding rates influenced yield structure elements of peas, protein percentage in seeds and yield of feed units. At seeding rates of 1.2-1.8 million of germinating seeds per hectare, there was an increase in protein percentage in seeds on more than 1.0% in comparison with the norms of 0.8-1.0 million of germinating seeds per hectare. In the same variants of the trial, the yield of digestible protein was at the level of 528-579 kg/ha, compared with that of 390-456 kg/ha when sown with a rate of 0.8-1.0 million of germinating seeds per hectare. The maximum productivity (2.07 t/ha) was recorded with a seeding rate of 1.4 million of germinating seeds per hectare. In the same variant of the trial, there were identified the maximum indicators of energy and economic efficiency, such as 2.07 of energy efficiency coefficient and 97.4% of profitability with the lowest production cost of 7600 rubles/ton.

Relationship of barley leaf’s pigment content with development of yield structure elements

Variation in contents of chlorophyll and carotenoid pigments and their correlation links with elements of yield structure of 17 samples of spring barley (Hordeum vulgare L.) was estimated by method of spectrophotometric analysis of acetone extracts from flag and second leaves. The variability of chlorophyll a content was 13.3%, of chlorophyll b – 13.5%, and carotenoids – 14.4% for flag leaf, and respectively 11.6%; 10.8%; 11.2% - for second leaf. Practically there was no variation in distribution of pigments between light-harvesting complexes (LHC) and reaction centers (RC) of photosystems (the average value of a part of chlorophyll in a light-harvesting complex was 81% of its total amount at a variation of 2.8%). The flag leaf pigments’ content was not correlated with final productivity of barley plants (r ≤ 0.27) whereas for a second leaf it is revealed statistically significant (at p ≤ 0.05) interrelation between productivity and content of chlorophyll a (r = 0.64), chlorophyll b (r = 0.58), and carotenoids (r = 0.60). It is disclosed that in the studied set of spring barley samples, one can predict extent of the level of the next parameters: “ear length”, “grain mass per ear” and “1000-grains mass” by the pigments content in a flag leaf; and the parameters “ear length” and productivity – by its content in a second leaf. Among three analyzed photosynthetic pigments, yellow pigments (carotenoids) render the greatest influence on the level of development of separate elements of yield structure. Content of pigments in a second leaf is more suitable index for assessment of potential productivity of spring barley cultivars. Breeding work on increase of carotenoids and/or chlorophyll b contents in a second leaf is perspective.

Effect of Attractants and Micronutrient Biofortification on the Yield and Quality of Red Clover (Trifolium pratense L.) Seeds

The aim of the research was to develop an innovative technique in the seed production of red clover. The research was carried out in 2013–2016, in Felin (51°22′ N, 22°65′ E), in a dependent arrangement (split-split-plot) in four replications. The first order factor was the use of an attractant: (1) control object, with pure water spraying, (2) attractant used in the 60 BBCH phase, (3) attractant applied twice—in the 60 and 65 phase, in the BBCH scale. The factor of the second order was fertilization: (a) standard object with PK fertilization; (b) fertilization with microelements (B + Mo) against the background of constant PK fertilization. The use of attractant ‘Pollinus’ preparation in the cultivation of red clover, both once and twice, in the form of spraying on plants, contributed to an increase in the seed yield, number of heads, share of seeds in the head, thousand seeds weight, and seed germination capacity, compared to the control object. Elements of the red clover yield structure achieved more favorable values with foliar feeding (B + Mo) compared to the standard one. Under the influence of plant biofortification with boron and molybdenum, the number of seeds infected with fungal pathogens and abnormally germinating decreased.

The effect of herbicides on the yield structure elements and the productivity of oil flax

The article provides the research results concerning the effect of antidicotyledonous herbicide Sekator Turbo, OD (a.i. amidosulfuron + iodosulfuron-methyl-sodium + mefenpyr-diethyl) in a tank mixture with various graminicides Quickstep, FEM (a.i. clethodim + haloxyfop-P-methyl), Bagheera, EC (a.i. quizalofop-P-tefuryl), Fuzilad Forte, EC (a.i. fluazifop-P-butyl), Miura, EC (a.i. quizalofop-P-ethyl), and Zellek-super, EC (a.i. haloxyfop-P-methyl) on the yield structure elements and the productivity of lowlinolenic (Nilin) and linolenic (FLIZ) varieties of oil flax on leached chernozem of the Western Ciscaucasia. We have found that the treatment of sowings with herbicides did not have a significant effect on the yield structure elements (thousand-seed weight and bulk weight of seeds). The treatment of sowings of the variety Nilin with the composition of Sekator Turbo + Bagheera or Sekator Turbo + Miura, and the variety FLIZ with Sekator Turbo + Bagheera, Sekator Turbo + Quickstep, Sekator Turbo + Fuzilad Forte, Sekator Turbo + Miura contributes to the receiving of oil flax yield at the level of the control with manual weeding. Tank mixtures of Sekator Turbo + Bagheera, Sekator Turbo + Miura decreased the oil content of seeds of the variety Nilin by 0.5 %. The application of herbicides Sekator Turbo, OD (0.1 l/ha) + Bagheera, EC (1.5 l/ha) provided the highest oil yield of the variety Nilin – 0.34 t/ha, and of the variety FLIZ – 0.54 t/ha. The composition of Sekator Turbo + Zellek-super led to a significant decrease in the productivity and oil content of seeds of the variety FLIZ – by 0.15 t/ha and 0.6 %, respectively.

Reaction of barley to top-dressing with different types of nitrogen fertilizer

The study was carried out to investigate effect of mineral (CAN) and organo-mineral (OMF) top-dress nitrogen fertilizers on physiological and morphological parameters in three barley cultivars. The study revealed significant differences in the reaction of the cultivars in magnitude and direction of the yield structure elements. CAN had smaller effect on the studied parameters than OMF. CAN had no effect on grain yield and straw mass. Only for the cv. Novichok, there was an increase in grain weight from the main ear (15%), the length of the main ear (15%), the 1000-grain weight (2.9%). OMF led to increase grain yield in the cv. Novichok (43%), Pamiaty Rodinoy (16%), and did not change in the cv. Rodnik Prikamya. Only the cv. Novichok has increased the grain mass from the main ear (17%). The length of the main ear increased in the cv. Novichok (13%), Pamiaty Rodinoy (11%) but decreased in the cv. Rodnik Prikamya (7.5%). The influence of OMF increased the 1000-grain weight in the cv. Novichok (4.9%) and Pamiaty Rodinoy (2.5%), but decreased in the cv. Rodnik Prikamya (1.5%). Statistically differences were revealed in reaction of pigment complexes of flag and second leaves in all cultivars.

Method of increasing yield indicators of rice varieties in process of seed production

The paper describes a method for creating new and increasing the yield of existing varieties based on analysis variability elements of rice varieties yield structure under influence of environmental conditions. Seeding rates are used as a source of variability. Following trait are analyzed: seeds field germination, survival plants before harvesting, total tillering of plants, realization of total tillering, graininess of panicle, fertility of spikelets, weight of 1000 grains. Based on analysis, through onnections conjugated elements, the optimal values of elements structure of yield are determined, at which it takes the maximum value. Based on a set of optimal values, theoretical model of studied rice variety is being developed. Then, according to panicles selected in variety, single-row plots are sown for further propagation, rows with parameters of the yield structure elements set same with model of variety are selected, which makes it possible to increase the yield of Mavr rice variety by 39.18 centners / ha or 45.61%.