Effect Of Moringa Oleifera Research Articles

Moringa oleifera L. mediated zinc oxide nano-biofertilizer alleviates non-alcoholic steatohepatitis via modulating de novo lipogenesis pathway and miRNA-122 expression

Nano-biofertilizer has recently been shown to alter plant growth through a variety of physiological and morphological changes which many improves the quality of plant and increase its active constitutes concentration. Moringa oleifera L. is a promising plant to treat non-alcoholic steatohepatitis (NASH) because of its potent anti-inflammatory, lipid-lowering, and antioxidant properties. Consequently, this research aims to investigate the impact of ZnO-nano-biofertilizer on Moringa oleifera L extract. The vegetative growth characteristics, and total phenolic and flavonoid contents were evaluated. Thirty-six rats were randomized to six groups and the effect of Moringa oleifera L. extract and Moringa oleifera L. extract with ZnO-nano-biofertilizer on rats fed with high-fat diet (HFD) was studied. The experimental animal results showed that Group (4) fed with HFD and Moringa oleifera L. extract with ZnO-nano-biofertilizer significantly improved liver functions, lipid profile, oxidative stress, inflammatory markers and possessed a superb effect considering the gross anatomical morphology and histopathological score of inflammation and steatosis as well as significantly reduced de novo lipogenesis and mi-RNA expression. In conclusion, ZnO-nano-biofertilizer improved the quality of Moringa oleifera L. and increased the its antioxidant activity. This effect positively reflected the improvement in the ability of Moringa oleifera L. extract to mitigate NASH in vivo.

Effect of moringa oleifera with pure and Zn-doped Mn and Ni nanoferrites for hyperthermia applications

Nanoferrites like spinel manganese ferrite (MnFe2O4) and inverse spinel nickel ferrite (NiFe2O4) have an efficient heating deportment to the destruction of neoplasm. The incorporation of Moringa oleifera (MO) leaf extracts with magnetic nanoparticles as heat mediators is an effective approach for treating magnetic hyperthermia. This study investigated the potential heating behavior of bare, Zn-doped MnFe2O4 and NiFe2O4 nanoparticles (NPs) with Moringa oleifera leaf extracts as magnetic hyperthermia agents. High-resolution transmission electron microscopy showed the spherical-like morphology of MnFe2O4 NPs with no impurities in the EDX spectra. X-ray diffraction spectra confirmed the cubic spinel and inverse spinel structure for MnFe2O4 and NiFe2O4 NPs, respectively. Magnetic properties of the prepared nanoferrites exhibit low coercivity values (in the range of 13–18 Oe representing superparamagnetic behavior. Further, the binding energies of the prepared nanoferrites were revealed by X-ray photoelectron spectroscopy. The effective magnetic anisotropy constant (Keff) was calculated to be around 3.9–5.9 kJ/m3 by electron spin resonance. An increased specific surface area (120.69 m2/g) and wider porosity (17.053 nm) were observed for MnFe2O4/MO NPs through Brunauer-Emmett-Teller (BET) analysis. Thermal properties were investigated by measuring the effective specific absorption rate in an alternating magnetic field ranging from 23 to 31 * 10−9 W/g Oe2 Hz. Results indicated that the green synthesized MnFe2O4/MO NPs are promising agents for magnetic hyperthermia applications in cancer therapy.

The effect of Moringa oleifera L. leaf extract supplementation on the chemical and sensory quality of beef patties cooked to a well-done level

This study aimed to evaluate the effect of Moringa oleifera L. leaf extract supplementation on the chemical composition and sensory quality of beef patties cooked to a well-done level. This study used a completely randomized design (CRD) in one direction with five treatments and five replications. The research treatments included the supplementation of Moringa oleifera L. leaf flour extract (MOE) 0% (P0), 1% (P1), 2% (P2), 3% (P3), and 4% (P4). The variables observed included water content, fat, protein, collagen, and sensory attributes. A 15-cm line scale was used to evaluate the intensity of the attributes tested on beef patties by 30 semi-trained panelists. Chemical and organoleptic quality test data were analyzed using Analysis of Variance. If the treatments had a significant effect, then the analysis was continued using the Tukey Test. The results showed that the addition of Moringa leaf flour extract had a very significant effect (P<0.05) on the chemical quality of beef patties (water, fat, and protein content). The use of Moringa oleifera L. leaf extract at the 2% level produces higher protein and fat levels than the use of 4%. Adding MOE up to 4% can increase the intensity of beef patties' color, aroma, and tenderness (P<0.05). The supplementation of Moringa oleifera L. leaf flour extract at the 2% level can produce beef patties with relatively high protein and fat content and can maintain sensory quality.

Effect of Moringa oleifera and Centrosema pubescence Leaf Meal as Diet Components on Haematological and Serum Biochemistry in Broiler Chicken

Effect of Moringa oleifera and Centrosema pubescence Leaf Meal as Diet Components on Haematological and Serum Biochemistry in Broiler Chicken

Effects of Moringa oleifera on 3, 4-Methylenedioxymethamphetamine (MDMA) Induced Neurotoxicity in the Pre-Frontal Cortex of Experimental Wistar Rats

Effects of Moringa oleifera on 3, 4-Methylenedioxymethamphetamine (MDMA) Induced Neurotoxicity in the Pre-Frontal Cortex of Experimental Wistar Rats

Comparative Repellency of Selected Plants to Adult Anopheles gambiea complex (Gilles) in the Laboratory

Aims: The study evaluated the repellency effects of some tropical plants and shrubs found in semi- rural communities of Badagry Area of Lagos state; which are acclaimed to have the potentials of repelling mosquitoes from human dwellings. The repellency effects of Moringa oleifera, Morinda lucida, Magnifera indica and Phyllanthus muellerianus to adult Anopheles gambiea was evaluated in the Laboratory.
 Study Design: The study was carried out at Central Research Laboratory of Lagos State University, Ojo, Lagos, Nigeria and Central Research Laboratory of University of Lagos, Akoka, Lagos, Nigeria. Powdered of dried test plants were prepared and admix with coconut husk as inert, different concentrations were rubbed on the forearm of volunteers and repellency to blood starved female Anopheles mosquitoes was observed.
 Methodology: Test plants were collected from Badagry area of Lagos State, they were identified at University of Lagos Herbarium and given numbers. They were dried between 10 and 14 days at temperature of 25-27oC and powdered. Different concentrations of the powder mixed with powdered coconut husk was used to treat volunteers forearms and they were exposed to 0-2 two day old adult unfed mosquitoes in an aluminum glass cage fitted with net as arm entrance and repellency was observed for a period of 180 minutes, with landing counts taken every 30 minutes. The test plants were also subjected to qualitative and quantitative phytochemical analysis at University of Lagos Central Research Laboratory.
 Results: Results showed that all test plants were able to repel Anopheles mosquitoes in the study, repellency was shown in descending order Moringa oleifera with 88%, Magnifera indica 83%, Phyllantus muellerianuss 80% and Morinda lucida 72%. There was no statistical significance in percentage repellency at 95% CL. The result of phytochemical screening of the test plants showed that only M .indica indicated presence of saponing (36.99%). While M.oleifera has highest phenol content (45.6%3), Alkaloid (38.68%), steroid (24.89%) and Tannin (33.19%). Flavonoid and reducing sugar quantity was highest in M. indica (39.39%) and (55.18%) respectively.
 Conclusion: The plants were able to show repellency to Anopheles gambiae a nuisance malaria vector of serious medical importance. These plants are available in all tropical areas of Africa, they can therefore be used to prevent nuisance and painful mosquito bites which could be a sustainable way to prevent mosquito vectored diseases.

Anti-Angiogenic Screening of Moringa Oleifera Leaves Extract Using Chorioallantonic Membrane Assay

Background: Angiogenesis is defined as the formation of new blood vessels. However, angiogenesis in cancer will lead to tumour growth and metastasis. Therefore, anti-angiogenesis is one of the ways to slow down growth and spreading of tumour. Moringa oleifera is also known as a “Miracle tree” which has high nutritive value and various therapeutics effect in different parts of the plant. This study aims to determine the anti-angiogenic property of Moringa oleifera leaves extract by using chick chorioallantoic membrane (CAM) assay. Materials and Methods: The extracts were prepared by decoction method using methanol and water. The qualitative phytochemical screening was carried out for both methanol and aqueous extracts. The fertilised chicken eggs were divided into six groups which include negative control group (phosphate-buffer saline with pH 7.4), positive control group (sunitinib), 50% and 100% methanol extract, 50% and 100% aqueous extract. The anti-angiogenic effect of Moringa oleifera leaves extract was determined by calculating the number and percentage decrease in blood vessels in post-24 and post-48 hours of treatment. Results: Statistical analysis by one-way ANOVA has shown significant (p<0.05) percentage reduction in the blood vessels between each treatment group after 48 hours of treatment. Among all the extracts, 100% aqueous extract of Moringa oleifera was found to have highest anti-angiogenic effect with the greater percentage decrease in blood vessels (81.33%) in post-48 hours of treatment. Furthermore, the anti-angiogenic effect of Moringa oleifera leaves was found to increased when the concentration of the Moringa oleifera extract was increased. Conclusion: Moringa oleifera leaves with various phytochemicals was found to possess anti-angiogenic potential.

Correction to: Effects of Moringa oleifera leaves extract, vitamin C, and taurine co-exposures on calcium and metallothionein levels, oxidative stress, and gill histopathological changes in Clarias gariepinus exposed to sub-lethal cadmium

Correction to: Effects of Moringa oleifera leaves extract, vitamin C, and taurine co-exposures on calcium and metallothionein levels, oxidative stress, and gill histopathological changes in Clarias gariepinus exposed to sub-lethal cadmium

The Effect of Moringa oleifera as a Primary Treatment in Urban Wastewater in Martínez De La Torre, Veracruz

Wastewater treatment is a priority, as most of this is discharged into rivers, lakes, seas, and soil. Since there is no treatment facility in Martnez de la Torre, Veracruz, these fluids are released straight into the Filobobos River without treatment. Hence, the Moringa oleifera seed was evaluated as a primary treatment. In this study using wastewater from a direct discharge, pH, turbidity, total suspended solids, and conductivity were determined as control measures. In the jar test, the treatments were carried out using different amounts of coagulant salts (aluminum and iron sulfate) and moringa powder; starting the agitation at 120 rpm for 5 minutes and immediately it was reduced to 60 rpm in 10 minutes with a rest time of 1 hour. After that, the quality parameters were analyzed. The moringa coagulant achieved an average maximum reduction of 71.84 per cent and 89.36 per cent in turbidity and Total Suspended Solids, respectively, which was higher than the salts used. Furthermore, its application had no effect on pH and conductivity parameters, and the coagulant based on Moringa oleifera as a primary treatment agent, since these qualities do not alter and post-treatment is not required, as in the case with salts.

Effects of Moringa oleifera in Two Independents Formulation and as Neuroprotective Agent Against Scopolamine-Induced Memory Impairment in Mice

BackgroundThe cognitive deficit has frequently been found in the elderly population. Several studies have shown that every single part of Moringa oleifera, including leaves, roots, and seeds, has abundant micronutrients, such as flavonoids, which improve the neurobehavioral capacity. However, herb parts that display optimal neuropharmacological properties remain unknown.ObjectiveWe investigate whether M. oleifera seed oil (MOO) or aqueous M. oleifera leaves extracts (MOEs) may ameliorate memory impairment in mice induced with scopolamine (Sco). Additionally, the phytochemical analyses of those two independent formulations were analyzed.MethodsIn this study, 2 ml/kg body weight (BW) of MOO and 500 mg/kg BW of MOE were orally administered to the mice for 28 days, followed by intraperitoneal injection of Sco (1 mg/kg) at the day 22–28 to induce cognitive impairment in those mice.ResultsThe Sco group showed memory retention impairment represented by the Y-maze and novel object recognition tests, significant enhancement of acetylcholine esterase (AChE) activity in hippocampus tissue (p < 0.0001), and increased the level of total antioxidant capacity (TAOC) in serum. Interestingly, the Sco-induced memory defect was improved and completely blunted the AChE exacerbation in Sco+MOO-treated mice (p < 0.0001), although the TAOC level was comparable among the groups. Mechanistically, both tropomyosin receptor kinase B (TrkB), as a brain-derived neurotrophic factor-receptor, and nuclear factor-kappa-light-chain-enhancer of activated B cells (NF-κB) protein expressions were enhanced with the hippocampus isolated from the Sco group. Nonetheless, pretreatment with MOO only, but not with MOE, ameliorated the enhanced protein expression levels of TrkB and NF-κB (p < 0.05 and p = 0.09, respectively).ConclusionOur data reveal that MOO is preferable to MOE as a neuroprotective as evidenced by improving memory impairment. This effect, at least in part, through inhibiting the AChE and NF-κB activities and modulating the TrkB expression level.

Effect of Moringa oleifera leaves on hematological profile of fluorosis affected rats.

Fluorosis is a metabolic disease that is endemic in nearly 25 countries with India being one of the most affected. It primarily affects the bone and the teeth. Moringa oleifera (MO) leaves are known to reduce the effect of fluorosis on various tissues. Therefore, it is of interest to document the effect of Moringa oleifera leaves on the hematological profile of fluorosis affected rats. Twenty four Sprague Dawley rats were housed two per cage in a room with 12 hours light and 12 hours dark cycle. The rats were allowed to adjust to the laboratory environment for about one to two weeks before the beginning of the study. This study reveals that MO leaves is effective in reducing the plasma fluoride content. It also helps in improving the Hb % and RBC count in fluorosis affected rats. Data shows that Moringa olifera leaves powder is effective in reducing the plasma fluoride content. It also helps in improving the Hemoglobin percentage & Red Blood Cell count in fluorosis affected rats.

Effects of Moringa oleifera, Curcumin, and Green Tea Extracts on Histopathological Changes in Mesenteric Lymph Nodes and Spleen of Albino Rats with Benzene-Induced Leukemia

Effects of Moringa oleifera, Curcumin, and Green Tea Extracts on Histopathological Changes in Mesenteric Lymph Nodes and Spleen of Albino Rats with Benzene-Induced Leukemia

Effects of Moringa oleifera on Follicular Development, Blood and Metabolic Profiles of Subtropical Ewes during Peripartum

Effects of Moringa oleifera on Follicular Development, Blood and Metabolic Profiles of Subtropical Ewes during Peripartum

ASSESSING THE EFFICIENCY OF MORINGA OLEIFERA LEAF MEAL ON THE GROWTH PERFORMANCE OF BROILER CHICKEN

High cost of poultry feed and limited fishmeal are currently the major challenges in poultry production. To reduce cost while maximizing production, there is the need to use cheap but high nutritional feed sources like Moringa oleifera. The aim of the study was to assess the effects of Moringa oleifera on the growth performance of broiler chicken by measuring their live weight, rate of mortality, feed conversion ratio and benefit cost (b/c) ratio. Field experiment was carried out at the Animal Science Department farm, located in the Kwame Nkrumah University of Science and Technology, Kumasi-Ghana. A total of 30-day old chicks were raised for eight weeks under the required conditions, with all vaccines administered appropriately. The experiment was laid in a Complete Randomized Design with five treatments namely T1= 100% conventional feed only (as control), T2= 50% MoLM (Moringa oleifera Leaf Meal) + 50% conventional, T3= 75% MoLM + 25% conventional, T4= 25% MoLM + 75% conventional, T5= 80% MoLM and each treatment replicated six times. The result showed no significant differences between the various treatment for the feed conversion ratio and live weight at different growth periods. The benefit/cost ratio of T1 was more than one while the other treatments were less than one. T4 (25% MoLM) had a b/c ratio close to one. In conclusion, Moringa oleifera leaf meal at different levels can be used to supplement the fishmeal component in the poultry diet of broiler chicken to produce similar results as that of the conventional feed. The study recommends that farmers can adopt Moringa oleifera based poultry feed for their bird production when they cannot afford the conventional feed (fish meal-based feed) to cut down cost economically while increasing productivity.

Effects of Moringa oleifera and Brosimum alicastrum partial feed substitution in intramuscular fat and adipose tissues and on the expression of lipogenic genes of Mexican hairless pigs

The present study evaluated the effects of the inclusión of ground Moringa oleifera and Brosimum alicastrum leaf meal in the diet of Mexican hairless pigs (MHP) on the amount of intramuscular fat, subcutaneous fat, leg muscle (Biceps femoris) fat, loin muscle (Longissimus dorsi) fat, leg and back fat, and the expression of lipid metabolism genes. Hairless pigs are reared in the Mexican tropics and are characterised by their body and intramuscular fat accumulation. Eighteen male pigs fed for 82 d were randomly allotted to three experimental isoenergetic and isoproteic diets, where M. oleifera or B. alicastrum (six pigs per diet) replaced wheat bran. The diets used were a control diet, a diet with 10% M. oleifera leaf meal, and a diet with 10% ground B. alicastrum leaf meal. The M. oleifera diet decreased (P<0.05) the fat ratio in the Longissimus dorsi muscles, back fat, rib fat, total carcass fat, and the carcass fat: meat ratio. The B. alicastrum diet only decreased fat in the Biceps femoris muscle, back fat, and rib fat. Moringa oleifera and B. alicastrum diets also promoted the overexpression of mRNA from the stearoyl-CoA desaturase (SCD), fatty acid synthase (FASN), acetyl-CoA carboxylase alpha (ACACA), sterol regulatory element-binding protein 1 (SREBP1) and acyl carrier protein (ACP) lipogenic genes in the Biceps femoris muscle and leg fat (P<0.001). In addition, lower ACACA and SREBP1 mRNA expression in the Longissimus dorsi muscle and back fat (P<0.001) were related to the lower amount of fat in pigs fed M. oleifera and B. alicastrum. The inclusion of Moringa oleifera and Brosimum alicastrum meals 10% in the diet of the pig MHP reduces fat, this is an important finding because fat is abundant in this type of pig.

Formulation, Optimization and Evaluation of Niosomes Containing Leaf Extract of Moringa oleifera and Pharmacological Screening of the Extract against Rheumatoid Arthritis

This research is formulated on the basis of the Rheumatoid Arthritis of a niosome that contains leaf extract of the herb. Therefore, in this part along with the medication system of the herb on the body, the pharmacologic testing procedure is clarified. In the following part, we will explore a thorough assessment of the condition, called rheumatoid arthritis and how the medicinal plant is used as a release agent. While focusing on rheumatoid arthritis throughout the discussion, the research discussion section has made it clear how effectively this paper has tried to meet the formation of niosome with Moringa extract and its effectiveness to reduce the pain. Furthermore, the entire analysis has been illustrated by focusing on the effect of Moringa oleifera over rheumatoid arthritis disease.

Neuroprotective Effect of Moringa Oleifera Extract on Acetamiprid Induced Neurotoxicity and Apoptosis in Albino Rats

Acetamiprid (ACMP) can cause neurotoxicity and induces neuronal apoptosis. The ameliorative effects ofMoringa Oleifera (MO) seed extract against the neurotoxicity induced by ACMP in adult male albino ratswere investigated. Forty male albino rats were divided into 4 equal groups. The first group served as a control,the second group administered ACMP 26.8mg/kg b.w. (representing 1/10 LD50) by gavage. The third groupwas given combination of ACMP 26.8 mg/kg b.w. and MO seed extract 150 mg/kg b.w. the fourth group wasgiven MO extract only 150 mg/kg b.w. for 28 consecutive days. Results proved the efficacy of MO extractas a neuroprotective agent through the reduction of the oxidant parameter malondialdhyde (MDA) contentby 26.9%, significant increase of reduced glutathione content (GSH) by 21.4%, and elevation of catalase(CAT) and superoxide dismutase (SOD) activities by (62.2% and 40.8%) respectively, compared to ACMPintoxicated group. Consequently, gene expression analysis for Bax and NBN genes was downregulatedby 54.5% and 36.3% respectively in MO treated rats (group III) compared with ACMP intoxicated group.Moreover, restoration of the pathological tissue injuries was noticed. In conclusion, MO proved to be aneffective neuroprotective and anti-apoptotic agent against ACMP-induced toxicity.

Moringa oleifera modulates cholinergic and purinergic enzymes activity in BV-2 microglial cells.

Microglia are immune cells that are resident in central nervous system. Activation of microglial cells are detrimental to the survival of neurons. Thus, prevention of microglia activation and/or protection against microglia activation could be potential therapeutic strategy towards the management of inflammation-mediated neurodegenerative diseases. Moringa oleifera is widely consumed as food and used in folklore medicine for treating several diseases. This study was convened to investigate the effect of aqueous extract of Moringa oleifera on cell viability, cholinergic and purinergic enzymes in BV-2 microglial cultured cell. Aqueous extract of Moringa oleifera was prepared, lyophilized and reconstituted in 0.5% dimethylsulphoxide (DMSO). Cells were treated with Moringa oleifera extracts (0.1-100μg/mL) and assessed for cell viability and nitric oxide production. Furthermore, the effect of Moringa oleifera on enzymes of cholinergic (acetylcholinesterase) and purinergic (nucleoside triphosphate diphosphohydrolase; NTPDase, 5' nucleotidase and adenosine deaminase; ADA) systems in BV-2 microglial cells were determined. Incubation of BV-2 microglia cell with M. oleifera extract maintained cell viability, modulated cholinergic and purinergic enzymes activity. The phenolic compounds found in M. oleifera extracts, include chlorogenic acid, rutin; quercetin pentoside, kaempferol derivative and quercetin derivative. Thus, this study suggest that the potential therapeutic effect of the phenolic compounds found in M. oleifera may have been responsible for the maintenance of cell viability in BV-2 microglia cells and modulation of cholinergic as well as purinergic enzymes activity.

Efficacy and safety on Moringa oleifera on blood glucose and lipid profile: A meta-analysis

Background: Results from previous clinical trials in which the effects of Moringa oleifera (MO) on blood glucose and lipid profile were investigated are controversial. Objectives: The main objective of this study was to assess the effects of MO consumption on blood glucose level and lipid profile in randomized controlled trial (RCTs) and non-RCTs. Materials and Methods: A comprehensive systematic review was performed by searching the PubMed, ScienceDirect, Scopus, and Thai Library Integrated System databases up to December 2019 without any language restrictions by two independent authors. The DerSimonian and Laird random-effects model method was used to pool the results. Results: Seven trials with 257 participants and treatment duration of 28–90 days were included. The pooled results showed a significant reduction in fasting blood sugar (FBS; weighted mean difference [WMD]: −14.81 mg/dL; 95% confidence interval [CI]: −27.99, −1.63; I2 = 97.8%), postprandial glucose (PPG) (WMD − 64.73 mg/dL; 95% CI: −102.87, −26.59; I2 = 93%) and no significant change in HbA1C (WMD: 0.70%; 95% CI: −1.42, 0.69; I2 = 99%), low-density lipoprotein (WMD − 11.20 mg/dL; 95% CI: −34.12, 11.72; I2 = 8.08%), total cholesterol (WMD − 4.73 mg/dL; 95% CI: −24.96, 15.49; I2 = 80%), and triglycerides (WMD − 3.29 mg/dL; 95% CI: −9.95, 3.36; I2 = 29%). Moreover, MO treatment increased high-density lipoprotein (HDL) level significantly (WMD 2.15 mg/dL; 95% CI: 1.92, 2.39; I2 = 0%). No serious adverse effects of the intervention were reported. Conclusion: The results of our study suggested that MO treatment decreased FBS, PPG levels and increase HDL level. However, the long-term benefits and safety of the treatment remain to be determined.

The Effect of Moringa Oleifera L. Against Serum Protein and Tissue in Pregnancy

Moringa leaves are known as herbs that have many properties.&nbsp;The study aimed to analyzed moringa leaf extract against tissue&nbsp;histology,&nbsp;serum protein levels, fetal conditions&nbsp;at birth from&nbsp;distended&nbsp;mice&nbsp;with&nbsp;malnutrition. The research design was a&nbsp;pretest post&nbsp;test&nbsp;control&nbsp;group design&nbsp;using 28&nbsp;distended mice&nbsp;with&nbsp;malnutrition, divided into 7 groups with 4&nbsp;mice&nbsp;- Group.&nbsp;In the treatment group given mo