Effects Of Ozone Therapy Research Articles

Insufficient evidence regarding the effects of ozone when used as a disinfectant in root canal therapy

Silva EJNL, Prado MC, Soares DN, Hecksher F, Martins JNR, Fidalgo TKS. The effect of ozone therapy in root canal disinfection: a systematic review [published online ahead of print October 6, 2019]. Int Endod J. https://doi.org/10.1111/iej.13229.

The effects of ozone therapy on postoperative adhesions and ovarian functions: An experimental study

Aim: Numerous methods are used to prevent the development of postoperative adhesion formation. There are few studies on the effects of ozone therapy on postoperative intraabdominal adhesions and ovarian functions. This study aimed to investigate the effects of ozone treatment on postoperative intraabdominal adhesions and ovarian roles in rats.Methods: Twenty female Wistar albino rats were randomly allocated into two groups as laparotomy (Group 1, n: 10) and laparotomy + intraperitoneal ozone (Group 2, n: 10). After laparotomy, parietal peritoneum, uterus and adnexal surfaces were scraped in both groups. Follicle-stimulating hormone (FSH), Estradiol (E2), Lactate Dehydrogenase (LDH), Urea, and Creatinine levels were measured, and histopathological evaluation was performed. Both groups were compared concerning histopathological and biochemical findings.Results: In the ozone-treated group, antioxidant levels (P=0.012) were significantly higher, and E2 levels (P=0.005) were substantially lower than the control group. There was no statistically significant difference between the two groups regarding FSH, Urea, Creatinine, and LDH (P=0.12, P=0.72, P=0.45, and P=0.79, respectively). Histopathologically, postoperative intraabdominal adhesion rates between the two groups were statistically similar.Conclusion: Although there was no statistically significant difference, ozone therapy had a decreasing effect on severe fibrosis and congestion rates. Although there was no difference in FSH, low levels of E2 in Group 2 suggest that ozone treatment may have a protective effect on the ovaries. However, further studies are needed concerning adhesion formation and the impact on ovarian functions.

The ameliorative effect of ozone therapy on spinal cord ischemia in rabbits

The ameliorative effect of ozone therapy on spinal cord ischemia in rabbits

Pulmonary effects of ozone therapy at different doses combined with antibioticotherapy in experimental sepsis model.

Purpose This experimental sepsis model created with Escherichia coli aimed to investigate the histopathological effects of two different doses of ozone combined with antibiotherapy on lung tissue.Methods Rats were divided into 5 groups. Then sepsis was induced intraperitoneally in the first 4 groups. The 1st group was treated with cefepime, the 2nd and 3rd groups were treated with cefepime combined with ozone at a dose of 0.6 mg/kg and 1.1 mg/kg. Lung tissue sections were stained with hematoxylin-eosin and assessed under light microscope and scored between 0-4 in terms of histopathological findings.Results In the comparisons between Group 1 and Group 4 in terms of cellular damage (p=0.030), inflammation (p=0.000) and overall score (p=0.007), statistically significant positive effects were observed in favor of Group 1. In the comparisons of Groups 2 and 3 with Group 4, only positive effects were observed in terms of inflammation (p=0.020, p=0.012, respectively).Conclusion Although negative histopathological effects of ozone on tissue injury were detected, it was noteworthy that the increase in the ozone dose reduced the number of damaged parameters.

Tavşan korneasında yüksek doz metilprednizolonun yanı sıra ozon (O3) tedavisinin hipoksi ile indüklenebilir faktör-1 alfa (HIF-la) ekspresyonu üzerine etkisi

Introduction: To investigate the effect of ozone (O3) therapy on cornea subjected to systemically used high dose methylprednisolone (MP) in a rabbit model. Methods: Twenty-four New Zealand White adult male rabbits were randomly divided into three equal groups as containing eight animals. The first group (n = 8) was used as the control group and nothing was applied to them, whereas the other 2 groups named as steroid groups were subjected to IM methylprednisolone injection at a dose of 20 mg/kg/day for three days. After three days of MP administration, only the third group was treated with 50-µg/mL O3 (20 mL O3) through the rectal insufflation for 14 sessions. The histopathological examination of corneas of three groups were made, and they were also assessed regarding the expression of hypoxia-inducible factor-1 α (HIF-1α). Results: It was determined that systemically administered high dose MP caused erosion and necrosis in corneal epithelium and stromal disintegrations in corneal stroma in steroid groups (Group 2 and Group 3). In the MP + O3 group (Group 3), the histopathological findings were mild. The expression of HIF1-α in the cornea of Group1 (control group), Group 2 (MP), and Group 3 (MP-O3) was measured as, 17.9±9.6%, 3.1±1.0% and 6.4±1.9% respectively. Conclusions: MP and MP-O3 therapy decreased HIF-1a expression in rabbit cornea in both intervention groups. Between these two groups, HIF-1α expression remained relatively high in the MP-O3 group than in the MP group alone.

The effect of ozone therapy on experimental bone fracture healing in rats [abstract

BACKGROUND: In the literature, many beneficial effects are attributed ozone; it balances the oxidant-antioxidant system, leads to delivery of super enriched oxygen at a cellular level and optimizes cell function, etc. PURPOSE: The aim of this study was to evaluate if there is a positive impact of ozone therapy on bone healing in an experimental fracture model in rats. MATERIAL and METHODS: After approval of experimental animal ethic committee, an open femoral fracture (ffx) was performed in 48 male Spraque Dawley rats. They were randomly divided into two groups: in Group O, medical ozone/oxygen mixture (500 mg/kg) and in Group C, medical air in volume of 4 mL were given rectally for 3-days. At days 4, 7 and 13, eight rats from each group were euthanized, and histomorphometric and immunohistochemical bone tissue evaluation was undertaken from the femur sampling. RESULTS: Histomorphometrically, periost thickness was thicker, trabecular areas were larger, cartilaginous and new bone areas were smaller (p

The effect of ozone therapy in root canal disinfection: a systematic review.

To answer the following focused question: 'As regards microorganism load reduction for patients undergoing root canal treatment, is the use of ozone therapy comparable to conventional chemomechanical techniques using sodium hypochlorite (NaOCl)?' A systematic review was conducted using controlled vocabulary and free-text key words in the following databases: PubMed, Science Direct, Scopus, Web of Science and Open Grey until 2 November 2018. Additional studies were sought through hand searching of endodontic journals. The inclusion criteria comprised studies that compared microbial reduction in root canals after treatments with ozone and NaOCl in extracted mature human teeth or randomized clinical trials. The quality assessment of included laboratory studies was performed with the following parameters: (i) sample size calculation, (ii) samples with similar dimensions, (iii) control group, (iv) standardization of procedures, (v) statistical analysis and (vi) other risk of bias. For randomized clinical trials, the qualitative analysis of the studies was performed from the bias risk assessment using the tool 'Bias Risk Assessment of Randomized Controlled Studies' Cochrane Handbook 5.0.2. The search resulted in 180 published studies. After removal of duplicate studies and full-text analysis, eight studies were selected and seven were considered low risk of bias (seven ex vivo studies and one random clinical trial). Overall, the results demonstrated that ozone therapy provides significantly less microbial load reduction than NaOCl. As an adjunct in chemomechanical preparation, ozone was ineffective in increasing the antimicrobial effect of NaOCl. Ozone performance was strongly associated with the application protocol used: it is dose, time and bacterial strain dependent, besides the correlation with the use of complementary disinfection sources. A restricted number of randomized clinical trial was found, and the difference amongst the methodology of the studies did not allow a meta-analysis to be performed. Although the selected studies had limitations, this review reached a satisfactory methodological and moderate evidence quality contributing to important preliminaryinformation regarding ozone therapy. As regards load reduction of microorganisms for patients undergoing root canal treatment, ozone is not indicated neither to replace nor to complement the antimicrobial action of NaOCl.

EVALUATION OF TOPICAL OZONE THERAPY ON SOCKET HEALING IN RABBITS WITH INDUCED DIABETES MELLITUS

INTRODUCTION: Ozone (O3) gas is a molecule consisting of three atoms of oxygen in a dynamically unstable structure due to the presence of mesomeric states. Ozone therapy has been utilized and heavily studied for more than a century. Its effects on bone are proven and consistent. It enhance osteoblastic activity and new bone formation. Diabetes mellitus (DM) is a chronic metabolic disease that potentially affect bone metabolism, structure, and mineral density. OBJECTIVES: to evaluate histologically the possible effect of ozone therapy on bone healing after tooth extraction in rabbits with alloxan induced diabetes mellitus. MATERIALS AND METHODS Thirty adult male New Zealand rabbits weighing 1500-2500 grams (approximately six months of age) will be divided into 3 equal groups: group I;(control group), group II; (diabetic group): diabetes was induced by a single intravenous injection of alloxan in a dose of 100 mg/kg and group III:(Ozone group) rabbits was treated as in diabetic group then they was treated with ozone therapy which will be applied topically in the form of gel inside the tooth socket. Rabbits were sacrificed after six weeks. Each sample were dissected and prepared for histological evaluation. RESULTS: in diabetic group the socket showed empty central region surrounded by disorganization bony trabeculae. The bony trabeculae surrounded less vascular spaces and more fibrous tissue.in diabetic group treated with ozone therapy the socket showed complete filling of the socket with newly formed bone.The socket with newly formed bone surrounding highly vascular bone marrow. CONCLUSIONS: Ozone gel application accelerates socket healing and enhances bone formation in diabetes mellitus

The effects of ozone therapy on wound healing after gingivectomy operations: a controlled clinical study

The effects of ozone therapy on wound healing after gingivectomy operations: a controlled clinical study

Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis.

In this study, the effects of ozonetherapy on secondary wound healing were evaluated histologically and immuno-histochemically. Material and Methods: 8 healthy pigs were used in this study. Six wounds with 10 mm in diameter were created through the punch technique on the palatinal gingiva of each pig. Ozone gas was applied on only 3 wounds (test group) and the remaining 3 were left to natural healing (control group). Biopsy samples were taken from one of the wounds in each group on the third day, from another wound of each group on the seventh day, and from another one on the tenth day. Routine histological analysis and immuno-histochemical staining were performed to investigate transforming growth factor-beta (TGF-β) and (VEGF) expressions. Results: No statistical difference was found between the test and control groups in terms of collagen fibers, epithelial formation and inflammation scores. A VEGF expression found in the test group was statistically higher than control group samples taken on the 3rd and 7th day. There was no statistical difference between the test and control groups in terms of TGF-β expression on any of the sampling days. Conclusion: The topical application of ozone gas could be effective in the early stages of wound healing by increasing the amount of VEGF expression. Clinical Relevance: Topical application of ozone gas may be effective in the early stages of oral wound healing.

Ozone Therapy in Knee Osteoarthritis: A Systematic Review

The aim of this study was to conduct a systematic review in order to examine the effectiveness of ozone therapy on knee osteoarthritis. The objectives were to evaluate the effect over time of ozone therapy in terms of knee pain, functional improvement and radiographic progression. A search was carried out on PubMed, Embase, Cochrane Library, Scopus and Web of Science databases to identify randomized and controlled studies focusing on this association. The following descriptors were used in English: ozone therapy, knee osteoarthritis. A descriptive summary and quality assessment was made of all studies included for analysis. Six randomized and controlled studies were identified. The risk of bias assessment demonstrated that one study was considered as having a moderate risk of bias and the remainder a high risk of bias. No quantitative analysis of the data was performed, as the studies included were not sufficiently homogeneous. The participants in the studies were generally elderly patients with mild to moderate knee osteoarthritis. The variability of ozone therapy and the comparators demonstrates that there is no standardized therapy. Few studies reported adverse effects, and where they occurred, they were mild and associated with the procedure. Ozone therapy proved effective in the short-term in relation to placebo and when combined with hyaluronic acid, but it was not superior to other current treatments. More randomised and controlled studies are needed to evaluate the risks/benefits of ozone therapy, both in the short term and the medium/long term.

OZONE THERAPY: TERATOGENIC STUDY OF OZONE. POSSIBLE INDICATIONS IN OBSTETRICS AND GYNECOLOGY

OZONE THERAPY: TERATOGENIC STUDY OF OZONE. POSSIBLE INDICATIONS IN OBSTETRICS AND GYNECOLOGY

The effects of ozone therapy as an adjunct to the surgical treatment of peri-implantitis.

PurposeThe decontamination procedure is a challenging aspect of surgical regenerative therapy (SRT) of peri-implantitis that affects its success. The purpose of the present study was to determine the impact of additional topical gaseous ozone therapy on the decontamination of implant surfaces in SRT of peri-implantitis.MethodsA total of 41 patients (22 males, 19 females; mean age, 53.55±8.98 years) with moderate or advanced peri-implantitis were randomly allocated to the test group (ozone group) with the use of sterile saline with additional ozone therapy or the control group with sterile saline alone for decontamination of the implant surfaces in SRT of peri-implantitis. Clinical and radiographic outcomes were evaluated over a period of 12 months.ResultsAt the 12-month follow-up, the plaque and gingival index values were significantly better in the ozone group (P<0.05). Probing depth decreased from 6.27±1.42 mm and 5.73±1.11 mm at baseline to 2.75±0.7 mm and 3.34±0.85 mm at the end of the 12-month observation period in the ozone and control groups, respectively. Similarly, the clinical attachment level values changed from 6.39±1.23 mm and 5.89±1.23 mm at baseline to 3.23±1.24 mm and 3.91±1.36 mm at the 12-month follow-up in the ozone and control groups, respectively. According to the radiographic evidence, the defect fill between baseline and 12 months postoperatively was 2.32±1.28 mm in the ozone group and 1.17±0.77 mm in the control group, which was a statistically significant between-group difference (P<0.05).ConclusionsImplant surface decontamination with the additional use of ozone therapy in SRT of peri-implantitis showed clinically and radiographically significant. Trial registry at ClinicalTrials.gov, NCT03018795.

Effectiveness of Ozone Therapy on Tendon Healing: An Experimental Study in Generated Achilles Tendon Injury Model in Rats

Effectiveness of Ozone Therapy on Tendon Healing: An Experimental Study in Generated Achilles Tendon Injury Model in Rats

Ozone Therapy: Overview of its Potential Utility in Male Reproduction

Ozone (O3), a highly water-soluble inorganic molecule, is a gas made of three atoms of oxygen (O) with a cyclic structure. Ozone can be produced by medical generators from pure oxygen after passing through a high voltage gradient. Ozone therapy (OT) can be given in medical practice via several routes that include transdermal, intramuscular, rectal, nasal, oral, vaginal, intravenous, intra-arterial, intraperitoneal, intra-pleural, topical, dental, intra-discal and by auto-hemotherapy. Because ozone, a highly reactive molecule, is a potent oxidant and anti-inflammatory agent, it has strong bactericidal, antiviral, anti-fungal and anti-protozoal actions as well as therapeutic effects on the immune system. With its multifaceted route of administration, OThas been used to treat several pathologies that involve the immune system such as cancers, sepsis, abscesses and chronic wounds, skin problems (such as eczema and psoriasis), HIV infection, asthma, arthritis, urologic problems, osteomyelitis and many others. The purpose of this review article is to evaluate the role of OT in the male reproductive system. We performed a review of all available basic science, experimental animal studies and clinical peer-reviewed articles published in PubMed and Google Scholar until November 2018. The literature so far retrieved shows that most studies pertaining to the effect of OT on male reproduction were performed in animals. Results to date show that OT, via improving the immune system, significantly protects testicular function in the setting of testicular torsion/ischemia, protects against the effect of gonadotoxic agents and treats bacterial infections in the semen. This article calls for a need for at least pilot studies in humans using OT in its safest route of administration, which is probably the transdermal one. This would be significant especially considering that male factor infertility constitutes up to one third of couple infertility and it is very common that poor semen parameters are irreversible with medical or surgical treatment, such as varicocele repair or vasectomy reversal.

Medical ozone promotes Nrf2 phosphorylation reducing oxidative stress and pro-inflammatory cytokines in multiple sclerosis patients

Oxidative stress and inflammation play key roles in the pathogenesis of Multiple sclerosis (MS). Different drugs have been used in the clinical practice, however, there is not a completely effective treatment. Due to its potential therapeutic action, medical ozone represents a promising approach for neurodegenerative disorders. The aim of the present study was to address the role of ozone therapy on the cellular redox state in MS patients. Ozone (20μg/ml) was administered three times per week during a month by rectal insufflation. The effect of ozone therapy on biomarkers of oxidative stress and inflammation was addressed by spectrophotometric and immunoenzymatic assays. Furthermore, we investigated the action of ozone on CK2 expression and Nrf2 phosphorylation by western blotting analysis. Medical ozone significantly improved (P < 0.05) the activity of antioxidant enzymes and increased the levels of cellular reduced glutathione. In accordance, a significant reduction (P < 0.05) of oxidative damage on lipids and proteins was observed in ozone-treated patients. As well, the levels of pro-inflammatory cytokines TNFα and IL-1β were lower after ozone treatment. Ozone therapy incremented the CK2 expression together with Nrf2 phosphorylation in mononuclear cells of MS patients. These findings suggest that ozone´s antioxidant and anti-inflammatory effects might be partially associated with an induction of Nrf2 phosphorylation and activation. These results provide new insights on the molecular events modulated by ozone, and pointed out ozone therapy as a potential therapeutic alternative for MS patients.

Comparison of Ozone and Photo-Biomodulation Therapies on Mental Nerve Injury in Rats

This study compared photo-biomodulation (PBM) and ozone therapy (OT) for mental nerve injury by counting Schwann cells (SCs) and fasciculated nerve branches and measuring fascicular nerveareas. The effects of OT and PBM on mental nerve injury were evaluated. Mental nerves of 27 rats were partly sutured and allocated into 3 groups. Group 1 received no treatment, group 2 received OT, and group 3 received PBM. The number of fascicules beyond nerve branches and the number of SCs before and after nerve injury were evaluated histologically. A better healing pattern was observed in the treatment groups. The number of SCs was markedly larger in the OT and PBM groups than in the control group. Oral and maxillofacial surgeons should be familiar with the differential diagnosis, prevention, and management of neurosensory disturbances. This study provides insights into the management of neurosensory disturbances related to mental nerve injury using OT and PBM. This study clearly suggests that OT and PBM are promising novel methods for the treatment of mental nerve injury.

The effectiveness of ozone therapy in treatment of acute osteomyelitis of jaws in children

The effectiveness of ozone therapy in treatment of acute osteomyelitis of jaws in children

Эффективность озонотерапии при лечении увеитов герпетической этиологии

Эффективность озонотерапии при лечении увеитов герпетической этиологии

Root Canal Irrigants and Dentin Bonding: An Update.

The purpose of the review was to assess the effect of root canal irrigants on dentin bonding. A PubMed-based search was conducted on the articles published from 1980 to 2016. A brief overview and reviewing the effect on dentin bonding of common root canal irrigation solutions such as sodium hypochlorite (NaOCl), chlorhexidine (CHX), ethylenediaminetetraacetic acid (EDTA), mixture of a tetracycline, acid and a detergent (MTAD) and ozone was conducted. Findings showed that, depending on the type of dentin bonding, using NaOCl may decrease, increase or not affect the bond strength. In addition, due to its broad-spectrum matrix metalloproteinase-inhibitory effect, CHX as well as MTAD can significantly improve the resin-dentin bond stability. However, the effect of ozone therapy on bond strength was controversial.