Effects Of Ozone Therapy Research Articles

The effect of Ozone therapy on pain perception after free gingival graft surgery in patients with mucogingival defects. A Randomized Controlled Clinical Trial.

The effect of Ozone therapy on pain perception after free gingival graft surgery in patients with mucogingival defects. A Randomized Controlled Clinical Trial.

Ozone therapy as a method of prevention and treatment in surgery and oncology (a literature review)

The effectiveness of ozone therapy in the treatment of cancer pathology lies in the proven process of destruction of atypical cells due to the inhibition of vicious anaerobic metabolism. Ozone selectively causes the death of cancer cells, since healthy cells have their own antioxidant defense system, while atypical cells do not. Ozone therapy for cancer pathology is an auxiliary treatment method that involves the effect of the oxygen-ozone layer on cancerous cellular structures. Numerous clinical studies confirm that ozone therapy is well tolerated and is extremely rarely accompanied by side effects and does not cause negative reactions in the human body. The advantages of ozone therapy include: safety for human health, minimal side effects, proven effectiveness, low cost of equipment and the possibility of use as cancer prevention. In addition, this treatment method is indicated for people suffering from chronic diseases and disorders that increase the risk of cancer, such as atherosclerosis, hypertension, type 2 diabetes, obesity, immunosuppression, menstrual disorders, etc. The use of ozone in medicine is very promising due to its anti-inflammatory, immunomodulatory and antitumor effects, and its hepatoprotective effect makes it possible to reduce the patient’s time in the hospital due to the rapid restoration of liver function in the postoperative period.

The protective effect of Ozone on the mice testicular damage induced by methotrexate.

Methotrexate (MTX) is widely administered for the treatment of various cancers. However, MTX induces male reproductive toxicity. In the current study, the effect of ozone therapy (OT) on reducing the toxic effects of MTX in the mouse testicles has been investigated. Twenty-four mice were divided into four groups: control, OT (4 mg/kg ozone), MTX (20 mg/kg), and MTX + OT. Testosterone levels, histological changes, and oxidative stress biomarkers were assessed to evaluate the protective effects of OT. The results demonstrated that MTX disrupted germinal epithelium, reduced serum testosterone levels, and enhanced oxidative stress in testicular tissue. However, treatment with OT attenuated these adverse effects. OT effectively restored the levels of antioxidant enzymes, such as catalase (CAT), glutathione (GSH), and superoxide dismutase (SOD). OT reduced lipid peroxidation, as indicated by decreased malondialdehyde (MDA) levels. OT preserved normal spermatogenesis, improved morphometric parameters, and reduced histological changes by MTX. Moreover, OT effectively restored testosterone levels. OT protects against MTX-induced testicular damage by suppressing oxidative stress.

Effect of Ozone Therapy on Pain, Depression and Quality of Life in Patients with Fibromyalgia Syndrome

Effect of Ozone Therapy on Pain, Depression and Quality of Life in Patients with Fibromyalgia Syndrome

Major clinical findings on the safety and effectiveness of ozone therapy in endodontic treatment: a concise systematic review

Introduction: In the scenario of endodontic diseases, microorganisms, and their byproducts are the main causes of pulpal and periradicular diseases. Sodium hypochlorite (NaOCl) is the most commonly used root canal irrigant. Ozone (O3) therapy has been investigated to reduce or eliminate the microbiota load within the root canal system, improving endodontic results. Objective: It was to highlight the main clinical findings of the safety and effectiveness of ozone therapy in endodontic treatment. Methods: The PRISMA Platform systematic review rules were followed. The search was carried out from July to September 2023 in the Scopus, PubMed, Science Direct, Scielo, and Google Scholar databases. The quality of the studies was based on the GRADE instrument and the risk of bias was analyzed according to the Cochrane instrument. Results and Conclusion: A total of 87 articles were found, and 23 articles were evaluated in full and 14 were included and developed in the present systematic review study. Considering the Cochrane tool for risk of bias, the overall assessment resulted in 18 studies with a high risk of bias and 21 studies that did not meet GRADE and AMSTAR-2. Most studies did not show homogeneity in their results, with X2=47.7%<50%. It was concluded that ozone has been accumulating scientific evidence regarding its success in endodontic treatment, more specifically in the decontamination of root canals and activation of regenerative processes. Ozone therapy is a minimally invasive treatment method, without discomfort or pain. It also minimizes the patient's anxiety and stress level, as it reduces the duration of treatment. The beneficial role of ozone in treating different oral and dental conditions is still limited. There are possible side effects during intraoral application, as it can reach the upper respiratory tract.

Effect of Ozone Therapy on Epidural Fibrosis in Rats

To demonstrate the effect of medical ozone therapy on the development of epidural fibrosis. A total of 25 Sprague-Dawley male rats were randomly divided into 3 groups: a control group (L3-L4 laminectomy only), a systemic ozone therapy (SOT) group (L3-L4 laminectomy only+ intraperitoneal 15 mL [30 μg/mL] ozone), and a local ozone therapy (LOT) group (L3-L4 laminectomy only+ subcutaneous 15 mL [30 μg/mL] ozone). Ozone therapy was administered 4 times on a 3-day interval during the wound-healing process, with the first dose immediately administered after surgery. The effects of ozone therapy on vascular endothelial growth factor, inflammation, and epidural fibrosis between groups were evaluated. Staining with vascular endothelial growth factor was significantly less in the group that received SOT compared with the control group (P= 0.021). When the groups were compared in terms of inflammation, it was found that inflammation was less common in the SOT and LOT groups compared with the control group (SOT vs. control: P= 0.004 and LOT vs. control: P= 0.024), whereas inflammation was found to be significantly less in the SOT group compared with the LOT group (P= 0.008). In the histopathologic evaluation of epidural fibrosis, there was no significant difference between the SOT and LOT groups but less epidural fibrosis was observed in both groups compared to the control group (LOT vs. control: P= 0.037; SOT vs. control: P= 0.018). Medical ozone therapy may be an alternative method that can be used effectively and safely in the prevention of epidural fibrosis after laminectomy.

Tactical and clinical positions to prevention wound complications in damaged soft tissues with staged adjuvant ozone-surgical treatment

The present review outlines features of a staged surgical tactics in treating battle and non-battle injuries in military medicine and disaster medicine. The problem of surgical infections in lightly wounded patients and the effectiveness of ozone therapy are also highlighted in the review. The authors introduce the concept of ozone-surgical treatment of wounds which is a combination of its evidence-based methods and acknowledged techniques of surgical care and blockades. The staged regional ozone therapy which is described in the article is an adjuvant surgical aid to prevent wound infection in damaged tissues.

Effects of ozone therapy on periodontal and peri-implant surgical wound healing: a systematic review.

To evaluate the effectiveness of the use of adjuvant ozone therapy in the healing process of wounds resulting from periodontal and peri-implant surgical procedures by answering the following focused question: "Can adjuvant ozone therapy improve wound healing outcomes related to periodontal and peri-implant surgical procedures?". MEDLINE (via PubMed), EMBASE, and Cochrane Central Register of Controlled Trials (CENTRAL) databases were searched, without language restriction, for peer-reviewed articles published until 23 March 2022, in addition to manual search. Only controlled clinical trials (randomized or not) were considered. The risk of bias was evaluated by the Cochrane risk-of-bias tool for RCTs - version 1 (RoB1). Data were pooled into evidence tables and a descriptive summary was presented. Of the 107 potentially eligible records, only seven studies were included. Four addressed free/deepithelialized gingival grafts with a palatal donor area, two evaluated implant sites, and one comprised gingivectomy and gingivoplasty. A total of 225 patients were evaluated in the included studies, considering control and test groups (ozone and other adjuvant therapies for comparison). Ozone therapy had a positive effect on outcomes directly or indirectly related to periodontal/peri-implant surgical wound healing. Furthermore, it could also increase the stability of immediately loaded single implants installed in the posterior mandible. In general, ozone therapy seems to both accelerate the healing processes of periodontal/peri-implant wounds and increase the secondary stability of dental implants; however, considering the limited evidence available and the risk of bias in the included studies (none classified as low risk), a definitive conclusion cannot be drawn. (Quintessence Int 2023;54: 100-110; doi: 10.3290/j.qi.b3512007).

The effectiveness of ozone therapy in splinting teeth.

Abstract: The purpose of this work is to study the condition of periodontal tissues during splinting of teeth. During the period of immobilization, along with the main treatment, it is necessary to carry out a set of measures aimed at preventing the progression of inflammatory and destructive changes in periodontal tissues. To prevent complications, various general and local drugs are used, in particular antiseptics, antibiotics and sulfonamide drugs. In recent years, various methods of modern physiotherapy have become increasingly used to prevent complications in dentistry, which determined the main content of this study. Key words: dental splinting, periodontitis, ozone therapy.

The Effectiveness of Ozone Therapy on Diabetic Ulcer Repair: A Systematic Review and Meta-analysis: Study of Changes in Wound Size

Introduction: Diabetic foot ulcer (DFU) is a typical dermatological complication often found in patients with diabetes mellitus (DM). Chronic hyperglycemia state causes microvascular impairment that leads to loss of peripheral sensory, motor, and autonomous functions. The current diabetic ulcer therapy has not yet provided a satisfactory reduction of the ulcer area; thus, several adjuvant therapies were tried, such as using ozone therapy, either systematically or topically. Ozone increases the cell membrane permeability to glucose, increases oxygen metabolism, improves oxidative preconditioning, stimulates endogenous antioxidant systems, and has anti-inflammatory and antibacterial effects. Methods: This study was an analytic observational study, with systematic review and meta-analysis. Data searching were conducted online at Pubmed-MEDLINE, Scopus, EBSCOhost, ProQuest, Cochrane library, ClinicalTrials.gov, and Google Scholar, as well as hand-searching from libraries in Indonesia. Five most relevant articles included in the qualitative (systematic review) (n = 449) and three of those five articles included in the quantitative analysis (meta-analysis) (n = 211). Reduction of ulcer area was assessed. Results: The meta-analysis of effectiveness of ozone intervention group compared to the control group showed a homogeneity in data (Q=95.547, df=2; p=0.000, Tau2=5.276). Meta-analysis showed the value of Q statistic was z value = -9.478; (p=0.000). This shows that the overall administration of ozone can significantly improve the diabetic ulcer repair. The overall standardized mean difference showed an increase in the reduction of ulcer area following ozone therapy was -1.740 (95% CI [-2.100 to -1.380], p=0.00). These results indicate that the ulcer healing of the intervention group was significantly better than the control group. Conclusion: Systematic review and meta-analysis results show that the application of ozone therapy significantly improves DFU repair.

Effects of Ozone Therapy on Chronic Arsenic Poisoning in Rats.

Arsenic (As) is a toxic metalloid that affects many organs through drinking water. This study aims to examine the efficacy of ozone therapy on chronic arsenic toxicity. Twenty-four male Wistar albino rats were housed in individual cages and grouped as control, As, O3, and As + O3. As was applied by adding 5mg/kg/day in drinking water for 60days. Ozone therapy was applied at 0.5mg/kg/day (i.p.) O3 in the last 5days of the experimental period. Tissues were harvested and analyzed for histopathological injury and apoptotic markers. There was no significant difference between the As + O3 and O3 groups (p = 0.186 and p = 0.599) for light microscopic criteria: inflammatory cell infiltration and hydropic degeneration in liver tissue.In TUNEL assessments, similar outcomes were obtained in the control and As + O3 groups. A statistically significant increase was observed in p53 and Caspase 3 (Casp-3) expression levels in the As group compared to the O3 and As + O3 groups. There was no significant difference between the As + O3 and O3 groups on peritubular hemorrhage and desquamation parameters in kidneys (p = 0.147 and p = 0.094). The KIM-1 expression level was significantly increased in the As group compared to the As + O3 group (p = 0.01), and the Casp-3 expression level was not significantly changed in the O3 group compared to the As + O3 group (p = 0.59). In conclusion, it is determined that ozone therapy has ameliorative effects on the microscopic injury of liver and kidney tissues. In addition to microscopic improvement, KIM-1 gene expression levels were ameliorated in the kidneys. The apoptotic cell counts and the Casp-3 and p53 gene expression levels were decreased by O3 administration. Thus, ozone therapy can be a treatment choice for As toxicity.

Effect of ozone therapy on the emotional state of a patient after the novel coronavirus infection

Purpose of the study. To assess the impact of systemic ozone therapy on the emotional state of patients after the novel coronavirus infection in the early recovery period. Materials and methods. A randomized controlled clinical trial, including 39 patients aged 29 to 78 years with a confirmed diagnosis of bilateral polysegmental viral pneumonia associated with SARS-CoV-2 coronavirus (J12.8), was conducted. The patients were divided into 2 statistically comparable groups. In the first group (N=19), the patients received a standard complex of rehabilitation treatment: exercise therapy, chest massage, and physiotherapy procedures (low-frequency magnetic therapy and drug electrophoresis in the projection of the lung fields), while the patients in the second group received daily intravenous infusions of ozonated saline solution with an output ozone concentration of 2.0 mg/l in addition to the basic complex rehabilitation. In order to assess the impact of systemic ozone therapy on the emotional state of the patients who underwent COVID-19, we analyzed the dynamics of complaints, changes in the quality of life of the patient based on the EQ-5D-3L questionnaire, as well as the severity of anxiety and depression according to the results of the HADS (Hospital Anxiety and Depression Scale) on the 1st and 10th days of the rehabilitation treatment. Results. In all patients, positive dynamics was noted against the background of rehabilitation measures in relation to complaints, the severity of anxiety and depression, as well as the quality of life. At the same time, the analysis of various indicators revealed greater efficiency in the prescription of a comprehensive program, including systemic ozone therapy. Conclusions. The use of ozone therapy in the complex rehabilitation of patients who have undergone COVID-19 has shown its efficiency and safety. Further study in higher number of patients is required to determine indications and criteria for its use.

Effect of intrauterine ozone therapy on Asherman syndrome, an experimental rat model

Background/aimsEvaluate the effect of ozone therapy alone or in combination with estradiol in management of Asherman syndrome in a rat model. Materials and methods48 rats were used and divided into eight groups. In all the groups right uterine horn was used for the experiment while the left horns received no action. Groups A underwent two operations, and groups B three times each. Group 1A and 1B were determined as the sham group. Ozone gas was given to Group 2 alone; estradiol orally was given to Group 3 alone; estradiol and ozone gas together was given to Group 4. ResultsIn ozone therapy group; the diameter of the uterus, the uterine lumen and the uterine glands were increased, the endometrial epithelium was thicker in the group that received double ozone application, and the number and diameter of the endometrial gland increased in the combined use of ozone and estradiol. In the rats who underwent two surgeries, the maximum number of pregnancies and advanced pregnancies were observed in the left uterine horn. The minimum number of pregnancies were achieved in estradiol alone group. In the group receiving combined treatment with ozone and estradiol, pregnancies were observed at a higher gestational age. ConclusionOzone had a positive effect on the treatment of Asherman syndrome and has a significant impact on achieving pregnancy.

Effects of ozone therapy on hematological and serum biochemical parameters in dogs affected by visceral leishmaniasis

Visceral leishmaniasis (VL) is a zoonosis and chronic disease caused by the protozoan Leishmania spp. which is of great concern to public health. Currently, the drugs used to treat the disease are costly and present undesirable side effects. The use of ozone to treat chronic diseases in both humans and dogs has shown satisfactory results. The aim of this study was to evaluate the ozone therapy in dogs with VL (n=6), administered by intra-abdominal application (IAA), to evaluate hematological and serum biochemical parameters. The protocol involves one gas dose per week for four consecutive weeks. The immunomodulatory action of ozone was determined in the laboratory analyses, confirmed by the increase trend in the white series and maintenance of the renal and hepatic function. Ozone therapy was found to be well tolerated and no adverse effects were observed. Further studies are required to elucidate the mechanisms of action on the infected host.

The Effects of Ozone Therapy on the Expression of IL-8, IL-10, CXCL9 and CXCL11 in Joint Fluid in Patients with Knee Osteoarthritis

Knee joint is one of the common sites of osteoarthritis (OA) with manifestation of localized joint swelling, pain and deformity, often seriously affecting daily work and life. It was found that the activation of Janus kinase / signal transducer and activator of transcription 3 (JAK / STAT3) signal pathway can lead to osteoarthritis. JAK-STAT signal transduction pathway is closely related to painful diabetic peripheral neuropathy and participates in neuropathic pain. Ozone therapy is often used for alleviating pain in neck, shoulder, waist and leg due to its oxidative, anti-inflammatory and analgesic effects. In recent years, ozone therapy is promising in treating knee osteoarthritis, which can significantly reduce the pain and swelling of the joints. The mechanism of ozone for knee osteoarthritis remains unclear and might associated with inhibition of inflammatory factors and matrix metalloproteinase activity, and intervention of free radical metabolism. In this study, the efficacy of ozone in the treatment of OA and its effect on inflammatory factors were evaluated by knee joint cavity ozone injection and detecting the expression of inflammatory factors IL-8, IL-10, CXCL9 and CXCL11 in joint fluid.

EFFICIENCY OF USING OZONE THERAPY IN TREATMENT OF INFLAMMATORY DISEASES IN GYNECOLOGICAL PATIENTS

Annotation: Examination and treatment of 85 women aged 18 to 40 years with acute and subacute inflammatory diseases of the genitals were carried out. All patients underwent a comprehensive clinical and laboratory study, including a general clinical examination, an assessment of the gynecological status with an ultrasound scan, a serological examination for chlamydia, viral infections, as well as a bacteriological and bacterioscopic examination of the vaginal flora and the detachable cervical canal. The patients were prescribed specific anti-inflammatory treatment, taking into account the etiology of the pathogen. Ozone therapy was used as an additional treatment in 45 patients (infusion of ozonized physiological solution of ORF, 200 ml, concentration 4 - 6 mg / l for 5 - 8 days, and in 40 cases - large ozonized autohemotherapy of BAP). A pronounced clinical effect of ozone therapy was noted in all patients: a rapid regression of pain and intoxication sy In addition, a significant positive dynamics of laboratory parameters was revealed (an increase in the percentage of lymphocytes, a decrease in the content of poorly differentiated forms of leukocytes, normalization of the protein fraction of blood serum). All patients with a specific etiology of the inflammatory process showed positive dynamics of specific immunological parameters of blood serum. ndromes, an improvement in overall well-being. Patients who received ozone therapy showed a rapid recovery of their general condition, which made it possible to shorten the hospitalization time. Bacteriological and bacterioscopic control after the treatment showed the absence of specific and nonspecific pathogens and the normalization of the vaginal microflora.

Comparative study of the effectiveness of ozone therapy and laser photobiomulation upon experimental wound repair: systematic review

Laser photobiomodulation and ozone therapy are therapeutic possibilities for antimicrobial control and biomodulation of tissue repair. This study aimed to compare the effect of laser photobiomodulation with that of ozone gas in wound healing of experimental lesions on animal's tissues. It was a systematic literature review, which aimed to search for articles of the animal model experimental study according the PRISMA guidelines. The search was made through the electronic articles indexed in the databases: PubMed, BVSalud, Cochrane, Scielo and Google Scholar, based on the DeCS/MeSH descriptors in English and Portuguese. The sample included studies from 2013 to 2020 and methodological quality was assessed using the ARRIVE guideline. A total of 329 of records indentified through database searching. Five studies were selected for the present systematic review according to the inclusion and exclusion criteria outlined for the research. All studies performed histological analysis of the treated tissue sections. Ozone therapy and laser showed a significant difference in favor of repair in different tissues when compared to the findings of the control groups in all studies. When compared to each other, ozone showed superiority over laser in two of the five studies, in addition to two studies having similar results between these two therapies. Regarding the ARRIVE guideline, in the most studies, the meeting criteria was high, with good methodological quality. Most studies highlighted the lack of uniformity in the therapy protocol as the greatest difficulty encountered, although they were unanimous in stating that laser photobiomodulation and ozone therapy contributed to the improvement of the healing pattern.

Effectiveness of Ozone Therapy in The Treatment of Endometritis in Mares

Endometritis is defined as inflammation of the endometrium that may be acute or chronic, infectious, or non-infectious. Endometritis is an important cause of subfertility in mares. Considering the antimicrobial characteristics, immune-stimulating ability, and low cost of ozone (O3) therapy, we aimed to evaluate the effectiveness of intrauterine O3 therapy as an alternative treatment for endometritis in mares. Twenty-five mares with a known reproductive history of uterine infection and inflammation were allocated into three groups: Group 1 (control; n = 7), uterine lavage with Ringer's lactate solution; Group 2 (O3-gas; n = 9), uterine lavage with Ringer's lactate solution followed by uterine insufflation with O2-O3 gas mixture containing 40 µg O3 mL−1 for 10 minute; and Group 3 (O3-oil; n = 9), uterine infusion of ozonized sunflower oil. Uterine inflammation was evaluated through a uterine cytological examination (cytobrush) and uterine culture (swab) for microbiological content before and after all treatments. In assessments of uterine cytology, the average number of neutrophils/field changed from 9.14 ± 3.02 to 7.71 ± 3.59 in the control group, from 10.67 ± 3.84 to 2.89 ± 3.59 in the O3-gas group, and from 6.44 ± 2.79 to 6.55 ± 7.18 in the O3-oil group post-treatment. The pre- and post-treatment findings in the mares treated with ozonized gas were significantly different (P < .05), unlike the findings for the mares in the control and O3-oil groups. All mares (25/25) showed a positive uterine culture before treatment. After treatment, the percentage of mares showing positive culture results was 57%, 11%, and 22% in the control, O3-gas, and O3-oil groups, respectively. Our results showed the effectiveness of two groups (O2-O3 gas mixture and ozonized sunflower oil) for the treatment of uterine infections in mares. Thus, direct intrauterine O3 gas infusion has been shown to be effective in treating endometritis in mares, reducing both inflammation and uterine infection.

Effectiveness of Ozone Therapy in the Treatment of Endometritis in Mares

Effectiveness of Ozone Therapy in the Treatment of Endometritis in Mares

Comparison of the effects of ozone therapy and photobiomodulation on sciatic nerve injury in rats

Comparison of the effects of ozone therapy and photobiomodulation on sciatic nerve injury in rats