Abstract
In this study, the effects of ozonetherapy on secondary wound healing were evaluated histologically and immuno-histochemically. Material and Methods: 8 healthy pigs were used in this study. Six wounds with 10 mm in diameter were created through the punch technique on the palatinal gingiva of each pig. Ozone gas was applied on only 3 wounds (test group) and the remaining 3 were left to natural healing (control group). Biopsy samples were taken from one of the wounds in each group on the third day, from another wound of each group on the seventh day, and from another one on the tenth day. Routine histological analysis and immuno-histochemical staining were performed to investigate transforming growth factor-beta (TGF-β) and (VEGF) expressions. Results: No statistical difference was found between the test and control groups in terms of collagen fibers, epithelial formation and inflammation scores. A VEGF expression found in the test group was statistically higher than control group samples taken on the 3rd and 7th day. There was no statistical difference between the test and control groups in terms of TGF-β expression on any of the sampling days. Conclusion: The topical application of ozone gas could be effective in the early stages of wound healing by increasing the amount of VEGF expression. Clinical Relevance: Topical application of ozone gas may be effective in the early stages of oral wound healing.
Highlights
Oral wound healing is a dynamic and complex process of restoring cellular structures and tissue layers1
In the test and control groups, it was observed that the surface epithelium and the lamina propria were completely separated from the wound area on day 3
It was noted that the connective tissue adjacent to the wound base was rich in neutrophils
Summary
Oral wound healing is a dynamic and complex process of restoring cellular structures and tissue layers. Oral wound healing is a dynamic and complex process of restoring cellular structures and tissue layers1 During this process, coordination occurs among epithelial cells, platelets, endothelial cells, fibroblasts and macrophages. Wound healing occurs in overlapping phases, which are inflammation, re-epithelialization, granulation tissue formation, matrix formation, and tissue remodeling. Re-epithelialization or epithelial healing is the most important and most complex of these processes. It includes cellular movement, proliferation and differentiation that provide functional and structural tissue repair. Secondary healing following surgical procedures, such as free gingival autograft, laterally sliding flap, gingivectomy and gingivoplasty, is always slower than primary healing
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