Objective: to investigate the relationship between the quality and duration of sleep and stress at work and at home in people aged 25–44 years living in Novosibirsk.Material and methods. The study of a representative cohort of people aged 25–44 years was conducted in 2013–2016 in one of the districts of Novosibirsk (budget topic No. AAAA-A17-117112850280-2). A total of 975 individuals were examined (427 men, mean age – 34±0.4 years, response rate – 71%; 548 women, mean age – 35±0.4 years, response rate – 72%). The general examination was conducted according to the standard methods of the WHO program “MONICA-psychosocial (MOPSY)”. The standard Jenkins questionnaire was used to assess the quality and duration of sleep. Scales to assess stress at home (scale “Knowledge and attitude towards one’s own health”) and at work (Karazek scale) were included in the standard questionnaire.Results. Men aged 25 to 44 with sleep disorders were more likely to report that they “dislike their work” (52.2%) and that their attitude towards work is “average” (50.7%). It is rare for both men (46.8%) and women (56.6%) to relax and have rest after a normal working day. Women with sleep problems (56.5 %) stated that they are unable to rest adequately at home. Both men and women with sleep disorders confirmed that there had been serious conflicts in the family in the last 12 months: “several” times" (57.1 % and 55.8 % respectively) or “often” (53.3 % and 68.4 % respectively). Women with a sleep duration of 5–6 hours stated that they “never” (36.4 %) or “rarely” (36.8 %) manage to relax and recover after a regular working day; for 33.3 %, responsibilities at work have “increased”; 37.2 % have started to do extra work.Conclusion. A correlation was found between sleep disturbances and their duration and stress both at work and at home.