Development Of Internal Organs Research Articles

Structural features of vestibule of the vaginas’ small glands in newborn girls with anomalies of internal genital organs

The purpose of the study was to identify the structural features of the small glands of the vestibule vagina with some anomalies in the development of internal genital organs. Material and methods. Glands of the vaginal vestibule of 18 newborn girls with internal genital anomalies were examined histologically using light microscopy. The comparison group consisted of 10 cases of newborn girls without pathological changes in the urogenital apparatus. After separation of the vestibule from the surrounding structures from the organ wall, longitudinal fragments were cut out, which were fixed in formalin and, after posting on alcohols, an increasing concentration was poured into paraffin. Sections 5-7 microns thick were stained with hematoxylin-eosin and by Van Gieson. Craigberg reaction was also conducted. The digital data obtained during the study were subjected to statistical processing. Results. The analysis showed that, in norm, the small glands of the vestibule of the child's vagina at the time of birth are capable of active secretion. In newborn girls with anomalies of the uro-genital system, the thickness and area of the initial section, the number of initial parts, the area of the initial part on the cut, the number of glandulocytes in the initial part of the glands decrease. There is an increase in the lumen of the common gland duct. Conclusions. Normally, small glands of the vestibule in the newborn are fully formed. With anomalies of the internal genital organs, significant destructive changes in the glandular apparatus of the vestibule are revealed. In contrast to the norm, the initial parts of the glands on the longitudinal sections are deformed. The thickness and area of the initial section decreases, the number of initial parts, the area of the initial part on the cut, the number of glandulocytes in the initial part of the glands.

Dietary or in ovo Saccharomyces cerevisiae Supplementation Developed Growth, Caecal Microbiota and Gut Histology of Broiler Chicks

The aim of this study was to determine the effects of in ovo injection of Saccharomyces cerevisiae (SC) to fertile Ross 308 broiler eggs or dietary supplementation on growth performance, internal organ development, gut histomorphology and cecum microbiota during 14 d of growing period. This study was designed as 2×2 factorial experimental design. Fertile 92 Ross 308 eggs were injected with SC solution 0.2ml at 18d of hatch and 90 eggs non-injected as control, afterward dietary SC supplementation was applied during the 14 d to broiler diet. In this study, 160 broiler chicks were used in four treatment groups at 10 replicate for each treatment group and 4 chicks for each replicates. Treatment groups were A) in ovo SC injection + dietary SC supplementation, B) in ovo SC injection + basal diet, C) no injection + dietary SC supplementation, D) no injection + basal diet as control. Live weight, feed consumption, feed conversion ratio and gut histomorphology, caecum microbiota, internal organ weight were recorded at 14 days of age. Live weight gain increased in C group than in the D group. Feed consumption, feed conversion ratio and internal organ weights were not affected by the treatments. Villi length and villi width increased in A group among the other treatment groups in jejunum, villi length increased in A and C groups among the other groups in ileum. Villi length/villi width ratio increased in A group than in the D group in ileum, was not different in jejunum. LAB counts in caecum were higher in A group than those of C and D groups, but was not different from B group. Enterobactericaea count was lower in A and B group than in the D group, was not different from C group. To conclude, results showed that dietary Saccharomyces cerevisiae supplementation increased broiler growth at 14 day by increasing villi development and improving gut health.

Situs Inversus Totalis in a 96‐Year‐Old Female Cadaver: Genetic Insights

BackgroundSitus inversus totalis is a complete reversal of thoracic and abdominal organs. Since only 25% of individuals with situs inversus have primary ciliary dyskinesia (PCD), which is a genetically heterogeneous disorder affecting motile cilia and is thought to be one of the underlining mechanisms of situs inversus, the majority of the latter cases still await their mechanistic elucidation. The present study reports the complete reversal of internal organs in a female cadaver 96 years of age and uses state‐of‐the‐art Next Generation Sequencing (NGS) to detect novel genetic mutations associated with situs inversus totalis.ResultsOur examination of the individual's body revealed that the visceral organs of the abdomen and thorax were mirror‐imaged to normal anatomy. The apex of the heart was directed to the right with a trilobed left lung and bilobed right lung. The liver was situated on the left side of the body, while the spleen and stomach were right sided. Several abdominal vasculature anomalies were also identified. In particular, the common hepatic artery branches from the superior mesenteric artery and the cystic artery branches from the right hepatic artery. There was also a common trunk that gives off the left gastric artery and an accessory right hepatic artery. In order to gain new insights into the molecular mechanisms that govern determination of the left/right asymmetry during the internal organ development, the genetic screen was performed using DNA extracted from the cadaveric tissue and the exome sequencing on the Illumina NGS platform.ConclusionThe present case can be described as situs inversus totalis which is accompanied by abnormal development of abdominal vasculature. The respective genetic analysis could point to a novel and additional to PCD mechanism(s) responsible for the aberrant visceral organ patterning and vascular development in humans.Support or Funding InformationThis study was supported by the Center for Anatomical Science and Education, Department of Surgery, Saint Louis University School of Medicine.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Morphobiological features of buck lambs by using high protein extruded food

The development of internal organs and the morphological composition of carcasses of the sheeps ox of Volgograd breeds when feeding them grain extruded forages with the use of dry beer pellets (EKKO-PD-SM) and pumpkin cake (EKKO-PC-SM) in combination with natural sulfur and methionine. Studies have established that sheep of experimental groups by the mass of internal organs were superior to the peers of the control group. Thus, the animals of the I and II-experimental groups had an advantage in comparison with the control peers: by weight of the leaked blood 9.24 and 11.54% (P ˂ 0.05); by weight of heart 12,41 and 16,47% (Р ˂ 0,05); by weight of lungs 8.60 and 11.34% (P ˂ 0.05); by weight of the liver 8,40 and 10,93% (Р ˂ 0,05); by weight of spleen 22,06 and 24,11% (Р ˂ 0,05) respectively. By the mass of the kidneys, there is no reliable difference in the compared groups, although the tendency of its increase in the animals of the experimental groups was noted. Buck lambs of I and II-experimental groups exceeded the control peer by weight of the stomach without contents by 8.04 and 10.57% (P ˂ 0.05), by weight of the small intestine by 9.07 and 12.29% (P ˂ 0.05), by weight of carcass (by 10.78-14.66%), by weight of pulp (by 17.19-22.81%), respectively.

THE STUDY OF EMBRYOTOXIC AND TERATOGENIC EFFECTS OF DIISONONYL PHTHALATE

Toxicological studies were conducted on impact of diisononyl phthalate (DINP) on the reproductive system of white vrats..In experiments, A. Dinerman,’s scheme was applied which allows recording embryotropic and teratogenic effects taking into account the dynamics of the development of offspring in the postnatal period. The presence of anomalies in the development of embryos internal organs was determined by the sagittal section method according to W. Wilson. It was found out that intragastric administration of the test compound to females during the pregnancy period at doses of 100, 1000 and 10,000 mg/kg initiated external and internal malformations in embryos. The exposure level of 10,000 mg/kg is characterized by increased rate of total post implantation and embryonic mortality, multiple embryonic developmental defects. At the same time, there were no significant changes in the postnatal development of the offspring compared to the control. In the experiment it was established that the dose of 10 mg/kg is the most inactive dose of DINP at which no signs of teratogenic and embryotropic actions were revealed.

Osteoblast-specific deletion of Hrpt2/Cdc73 results in high bone mass and increased bone turnover

Inactivating mutations that lead to loss of heterozygosity within the HRPT2/Cdc73 gene are directly linked to the development of primary hyperparathyroidism, parathyroid adenomas, and ossifying fibromas of the jaw (HPT-JT). The protein product of the Cdc73 gene, parafibromin, is a core member of the polymerase-associated factors (PAF) complex, which coordinates epigenetic modifiers and transcriptional machinery to control gene expression. We conditionally deleted Cdc73 within mesenchymal progenitors or within mature osteoblasts and osteocytes to determine the consequences of parafibromin loss within the mesenchymal lineage. Homozygous deletion of Cdc73 via the Dermo1-Cre driver resulted in embryos which lacked mesenchymal organ development of internal organs, including the heart and fetal liver. Immunohistochemical detection of cleaved caspase-3 revealed extensive apoptosis within the progenitor pools of developing organs. Unexpectedly, when Cdc73 was homozygously deleted within mature osteoblasts and osteocytes (via the Ocn-Cre driver), the mice had a normal life span but increased cortical and trabecular bone. OCN-Cre;Cdc73flox/flox bones displayed large cortical pores actively undergoing bone remodeling. Additionally the cortical bone of OCN-Cre;Cdc73flox/flox femurs contained osteocytes with marked amounts of cytoplasmic RNA and a high rate of apoptosis. Transcriptional analysis via RNA-seq within OCN-Cre;Cdc73flox/flox osteoblasts showed that loss of Cdc73 led to a derepression of osteoblast-specific genes, specifically those for collagen and other bone matrix proteins. These results aid in our understanding of the role parafibromin plays within transcriptional regulation, terminal differentiation, and bone homeostasis.

Body Conformation and Internal Organs Characteristics of Different Commercial Broiler Lines

ABSTRACT The aim of the study was to determine body weight and dimensions, body conformation, length of the esophagus, length of intestine and its segments, as well as weight of internal organs and their proportions relative the body weight of broiler chickens from three commercial lines - Ross 308, Hubbard Flex and Hubbard F15. At the age of 42 days, Ross 308 chickens had significantly (p≤0.05) shorter trunk, but greater chest circumference and compactness index, as well as shorter esophagus and longer large intestine compared with Hubbard F15. The longest large and total intestine was found in Ross 308 broilers. Chicken genotype had no significant effect on the percentage of the main internal organs, i.e. liver, heart, proventriculus, gizzard, and spleen. In the analyzed broilers, the coefficients of correlation between body weight and dimensions and the length of the esophagus, small intestine, caeca and large intestine were low and not significant. This study provides information relevant to breeding practice, including that the development of internal organs in broiler chickens raised under intensive conditions has an effect on their meat characteristics.

Effects of medicinal plants, Alisma canaliculatum, Laminaria japonica and Cornus officinalis, treated with probiotics on growth performance, meat composition and internal organ development of broiler chicken

A trial was conducted with 175 day old Ross broiler chicks to evaluate the effectiveness of Alisma canaliculatum (Water plantain or Theksa), Laminaria japonica (Sea tangle or Dashima) and Cornus officinalis (Cornelain cherry or Sansuyu) medicinal plants treated with probiotics in replacing antibiotic. Three medicinal plants were prepared in dry and flour form with treatment of probiotics. The groups were control (basal diet), antibiotic (basal diet + 0.05% OTC, oxytetracycline), Water plantain treated with Probiotics (WPP) 0.5%, Sea tangle treated with Probiotics (STP) 0.5% and Cornelian cherry treated with Probiotics (CCP) 0.5% in basal diet. The broilers were distributed in a completely randomized design having 5 replications with 7 chicks per replication in wire cage. The data were analyzed by SAS package program. Addition of three medicinal plants in broiler diets didn’t show negative effect on growth and feed conversion efficiency in broilers. Significantly highest protein percentage in broiler meat was found in groups fed the three medicinal plants treated with probiotics (WPP, STP and CCP) compared with other groups (P<0.05). Crude ash also increased significantly by the three medicinal plants addition compared to control especially in breast meat (P<0.05). Addition of probiotics treated with medicinal plants had similar beneficial effects on most of the observed parameters to those found in antibiotic-fed groups, so it implies that these three medicinal plants can be the possible alternatives to antibiotic for broiler production.Asian J. Med. Biol. Res. December 2016, 2(4): 696-702

Effect of early feed restriction on physiological responses, performance and ascites incidence in broiler chickens raised in normal or cold environment

Intensive selection of broilers for faster growth and better feed efficiency resulted in greater susceptibility to metabolic disorders such as ascites syndrome, which is one of the major causes of mortality and economic loss in broiler industry. Whereas cool temperature is one of the primary triggers for ascites, early feed restriction (FDR) significantly alleviates its incidence and mortality. However, little is known about effects of FDR, cold environmental temperature and their interaction on physiological responses in broiler chickens. For this purpose, 320 one-day-old male broilers were divided into two treatment groups of Ad libitum (Ad) and feed restricted (FR) with eight pen replicates each. Chickens in FR group underwent feed access limitation from days 7 to 14 of age. On day 21 half of the birds (four pens) in each group exposed to the cold temperature (CT) and the other half (four pens) continued at normal temperature (NT). Average daily feed intake, average daily weight gain and feed conversion ratio (FCR) were measured at days 7, 14, 21, 28 and 42. At 39 and 46 days of age two chicks with a BW around the pen average were selected from each pen and slaughtered after collecting blood samples. Then, relative weight of internal organs and right ventricle weight per total ventricle weight (RV : TV) ratio were calculated. Compared with NT group, CT birds had higher daily feed intake and FCR (P<0.05) from day 28 to 42. Cumulative ascites mortality in CT chickens was higher (P<0.001) than NT chicks. Within the CT group, ascites mortality in FR chickens was reduced (P<0.001) to 1.25% compared with 8.75% in Ad chicks. Birds in CT group had significantly (P<0.05) thicker right ventricle and greater relative weight of heart, hematocrit and triiodothyronine concentration. However, none of these parameters were affected by FDR. Under cold stress conditions, FDR reduced activity of alanine aminotransferase and aspartate aminotransferase (P<0.05). Serum triglyceride, cholesterol, high-density lipoprotein and total protein were not influenced by either temperature or feeding regimen. In conclusion, these findings suggest that FDR reduces ascites incidence mainly by allowing better development of internal organs, which helps them to cope with the high metabolic pressure and suffer less damage.

Effect of Different Combinations of Unboiled and Boiled Tomato Waste in Diet on Performance, Internal Organ Development and Serum Lipid Profile of Broiler Chicken

Effect of Different Combinations of Unboiled and Boiled Tomato Waste in Diet on Performance, Internal Organ Development and Serum Lipid Profile of Broiler Chicken

Review of terminology for the outline of dorsal scutum in Laniatores (Arachnida, Opiliones).

In many Opiliones (notably the Laniatores) the five most anterior opisthosomal tergites are fused with the carapace forming the so called dorsal scutum (DS) (Latreille 1804; Simon 1879; Hadži 1942) with a highly variable shape arising from multiple factors, such as differential development of musculature (especially of coxa IV), internal organs and influence of appendages (Loman 1903; Winkler 1957). The different degrees of fusion of the tergites were first studied by Hadži (1942), who proposed a terminology for them. This terminology was adopted and enhanced by Kratochvíl (1958) and Martens (1978). A shield formed by the fusion of the carapace with abdominal tergites I to V is called scutum magnum (Hadži 1942). The shield formed by the fusion of carapace with abdominal tergites I to VII is called scutum complexum (Kratochvíl 1958) and occurs in the males of Heteropachylinae Kury, 1994 (Kury 1994) and Paralolidae Kratochvíl, 1958 (Kratochvíl 1958). Finally, the scutum completum (Hadži 1942) is formed by the complete fusion of the carapace and abdominal scutum, formed by tergites I to VIII, and occurs in the Sandokanidae (Martens 1978). In this paper we focus on the different forms of the scutum magnum.

Effects of infestation by Argeia pugettensis Dana, 1853 (Isopoda, Bopyridae) on the growth and reproduction of the sand shrimp Crangon hakodatei Rathbun, 1902 (Decapoda, Crangonidae) in South Korea

This study investigated possible parasitic effects on growth, reproduction and external sexual characters induced by a bopyrid isopod Argeia pugettensis Dana, 1853, which parasitizes the branchial chamber of the sand shrimp Crangon hakodatei Rathbun, 1902 in South Korea. Total infestation rate was 5.7%. The sizes of infected female and male hosts were similar compared to those of uninfected hosts. A. pugettensis showed size-specific selection for hosts. The relationship between host and parasite length was significantly positive. Several effects of parasitization were observed on C. hakodatei parasitized by A. pugettensis. In female hosts, parasitic effects were observed in growth (increased growth), internal organ development (immature gonads) and external sexual characters (no breeding dress). In male hosts, parasitic effects were observed on growth (increased growth) and development of external sexual characters (smaller appendix masculina).

Multi-breed genome-wide association study reveals novel loci associated with the weight of internal organs.

BackgroundRecently, many genome-wide association studies (GWAS) have been conducted to understand the genetic architecture of economic important traits in farm animals. Pig is widely used as a biomedical animal model for its similarity with humans in terms of organ formation and disease mechanisms. Moreover, understanding the mechanisms that underlie the development of internal organs will impact the productive potential of pigs. Our aim was to uncover new single nucleotide polymorphisms (SNPs) associated with the weight of internal organs and carcass and also potential candidate genes.MethodsWe performed GWAS for the weight of heart, liver, spleen, kidney and carcass on five pig populations (White Duroc × Erhualian F2 intercross, Sutai population, Laiwu population, Erhualian population and commercial population, for a total of 2650 individuals). Genotype data was produced using the PorcineSNP60 Beadchip array. After quality control, the data was used for association tests under a general linear mixed model. Population stratification was adjusted by including a random polygenic effect based on a matrix of genotypic relationships. A meta-analysis of our GWAS datasets was conducted by summing up the Chi square values across breeds, with the degrees of freedom of the Chi square distribution equal to the effective number of breeds.ResultsThirty-nine quantitative trait loci (QTL) located on 15 chromosomes were identified by the single-population GWAS at the suggestive level. Among these, nine QTL surpassed the 5 % genome-wide significance threshold, including four for heart weight on SSC (Sus scrofa chromosome) 2, 4, 7 and 10, two for liver weight on SSC7, two for spleen weight on SSC5 and SSC7 and one for carcass weight on SSC11. The QTL on SSC7 showed pleiotropic effects for heart, liver and spleen weights in the F2 population. In addition, two QTL were detected in several populations, including one on SSC2 for heart weight in the F2 and Sutai populations and one on SSC7 for liver weight in the F2 and Laiwu populations. The meta-analysis detected four novel QTL on SSC1, 3, 8 and 16 for carcass weight.Electronic supplementary materialThe online version of this article (doi:10.1186/s12711-015-0168-7) contains supplementary material, which is available to authorized users.

Loss of cftr function leads to pancreatic destruction in larval zebrafish

The development and function of many internal organs requires precisely regulated fluid secretion. A key regulator of vertebrate fluid secretion is an anion channel, the cystic fibrosis transmembrane conductance regulator (CFTR). Loss of CFTR function leads to defects in fluid transport and cystic fibrosis (CF), a complex disease characterized by a loss of fluid secretion and mucus buildup in many organs including the lungs, liver, and pancreas. Several animal models including mouse, ferret and pig have been generated to investigate the pathophysiology of CF. However, these models have limited accessibility to early processes in the development of CF and are not amenable for forward genetic or chemical screens. Here, we show that Cftr is expressed and localized to the apical membrane of the zebrafish pancreatic duct and that loss of cftr function leads to destruction of the exocrine pancreas and a cystic fibrosis phenotype that mirrors human disease. Our analyses reveal that the cftr mutant pancreas initially develops normally, then rapidly loses pancreatic tissue during larval life, reflecting pancreatic disease in CF. Altogether, we demonstrate that the cftr mutant zebrafish is a powerful new model for pancreatitis and pancreatic destruction in CF. This accessible model will allow more detailed investigation into the mechanisms that drive CF of the pancreas and facilitate development of new therapies to treat the disease.

Developmental and morphological studies in Japanese medaka with ultra-high resolution optical coherence tomography.

We propose ultra-high resolution optical coherence tomography to study the morphological development of internal organs in medaka fish in the post-embryonic stages at micrometer resolution. Different stages of Japanese medaka were imaged after hatching in vivo with an axial resolution of 2.8 µm in tissue. Various morphological structures and organs identified in the OCT images were then compared with the histology. Due to the medaka's close resemblance to vertebrates, including humans, these morphological features play an important role in morphogenesis and can be used to study diseases that also occur in humans.

Pattern Formation of an Epithelial Tubule by Mechanical Instability during Epididymal Development

A single epithelial tubule undergoes morphogenesis to form a functional shape during the development of internal organs; however, the mechanical processes that are directed by the molecular signals regulating tubular morphogenesis are poorly understood. Here, axial tubular buckling triggered by cell proliferation is shown to drive the morphogenesis of murine epididymal tubules through mechanical interactions between the developing epithelial tubule and its surrounding tissues. Through immunofluorescence labeling and mathematical modeling, epididymal tubule shape formation is found to depend on two factors: cell proliferation area in the tubule and mechanical resistance from the tissues surrounding the tubule. Moreover, experimental perturbations of these two factors alter the shape of the epididymal tubule as predicted by the mathematical model, suggesting that the shape of the epididymal tubule spontaneously emerges through mechanical coupling between developing tissues instead of by growing according to a predetermined fate.

Effect of Herbal Essential Oil mixture on Intestinal Mucosal Development, Growth Performance, and Weights of Internal Organs of Quails

The aim of this study was to examine the effects of herbal essential oil mixture on intestinal mucosal development, weights of the internal organs, carcass characteristics, and growth performance of quail (Coturnix coturnix japonica). A total of 276 quails that were 1 day old were studied. The quails were weighed and divided into 3 groups, each with 4 replicates, consisting of a control, antibiotic (avilamycin 10 mg/kg diet), and the essential oil mixture (EOM) at 48 mg/kg diet. Supplementation with EOM and antibiotic significantly increased goblet cell size and goblet cell number on day 38. Villi height, villi width, and crypt depth were not affected by any treatment. Quails receiving the diet containing EOM and antibiotic were significantly heavier than those fed the control diet on days 28 and 38. No significant effects of the dietary treatments on feed intake were observed. Moreover, addition of EOM and antibiotic to the quail diet resulted in a significantly better feed conversion ratio than that of the control on days 28 and 38. No differences were observed for carcass yield or liver, gizzard, and small intestine weights. Beneficial effects of the EOM were observed in goblet cells of the intestinal mucosa.

Protective Immune Response against Bacillus anthracis Induced by Intranasal Introduction of a Recombinant Adenovirus Expressing the Protective Antigen Fused to the Fc-fragment of IgG2a

Anthrax is a particularly dangerous infectious disease that affects humans and livestock. It is characterized by intoxication, serosanguineous skin lesions, development of lymph nodes and internal organs, and may manifest itsself in either a cutaneous or septic form. The pathogenic agent is Bacillus anthracis, a grampositive, endospore-forming, rod-shaped aerobic bacterium. Efficacious vaccines that can rapidly induce a long-term immune response are required to prevent anthrax infection in humans. In this study, we designed three recombinant human adenovirus serotype-5-based vectors containing various modifications of the fourth domain of the B. anthracis protective antigen (PA). Three PA modifications were constructed: a secretable form (Ad-sPA), a non-secretable form (Ad-cPA), and a form with the protective antigen fused to the Fc fragment of immunoglobulin G2a (Ad-PA-Fc). All these forms exhibited protective properties against Bacillus anthracis. The highest level of protection was induced by the Ad-PA-Fc recombinant adenovirus. Our findings indicate that the introduction of the Fc antibody fragment into the protective antigen significantly improves the protective properties of the Ad-PA-Fc adenovirus against B. anthracis.

Ukształtowanie ciała i morfometria wybranych narządów wewnętrznych przepiórek Faraon w różnym wieku

The effect of age of Pharaoh quail on body weight, body conformation and development of internal organs, including the digestive tract, was determined. At the age of 35 days, Pharaoh quail were characterized by significantly lower body weight, length of trunk with neck, and length of trunk compared to 45-day-old birds. Younger quail had slightly shorter breast bone and lower thighs, smaller chest girth, and the same length of shanks. Body conformation indices were significantly (P?0.05) higher in 35-day-old birds. Pharaoh quail, evaluated at 35 and 45 days of age, did not differ significantly in the length of the oesophagus and crop, the length of intestine and its segments, and the weight and proportion of gizzard, proventriculus and heart. As birds aged, liver weight increased significantly, as did the weight and proportion of testicles in males.

HISTOLOGICAL STUDY ON INTERNAL ORGANS DEVELOPMENT OF MANGROVE SNAPPER, Lutjanus argentimaculatus LARVAE

Mangrove snapper, Lutjanus argentimaculatus is one of highly economic value of marine fish commodity. Culture of this species has been developed a few years ago. Basic data on biological aspect is still limited whereas those kinds of information are needed. Histological study to determine development of internal organs of the larvae of mangrove snapper was conducted. Amount of 15-20 larvae each from 1 day after DAH hatching (1 DAH) up to 30 days after hatching (30 DAH) were collected, fixed in Bouin’s solution for 4-6 hours and then transferred into 70% alcohol. The samples were then embedded in paraffin wax and the sections were stained with haematoxylineosin (H&amp;E). Results showed that the eye retina of the mangrove snapper larvae well developed on 3 DAH, whereas their digestive tract was on 5 DAH. Under this condition, the larvae started feeding as showed by present of natural food tissues in the intestine. Development of digestive tract and other internal organs reached their complete form like in adult fish after 20 DAH.