The first step in the development of biochips is to prepare biochip carriers. In this process, the surface of the carrier needs to be chemically treated to enable rapid and efficient immobilisation of the various probes. The quality of the carrier directly affects the performance of the microarray and is critical to the preparation of the chip. In this paper, the surface-smoothed slides were used to assemble nanofilms with large specific surface area and super-adsorption properties on the slides, and then functionalised surface modification, giving full play to the dual advantages of physical adsorption and chemical coupling. The glass cuboid is used as the chip substrate, and the cylindrical glass capillary is used as the microfluidic channel. By simply combining it with the flow pump, a three-dimensional microfluidic immunoassay device is constructed. By comparing the properties of protein immobilisation, immune response efficiency and signal-to-noise ratio in three-dimensional devices, it is compared with traditional two-dimensional microfluidic chips. The scores in the experimental group and the control group were significantly lower than those in the initial diagnosis, P 0.04, indicating that there was no statistical difference between the two groups. The use of topical drugs to treat chronic localisation problems can effectively alleviate the patient's discomfort and reduce the symptoms of skin lesions. At the same time, it has high safety and good promotion value.