Concentrations Of Iron Oxide Nanoparticles Research Articles

Lauric acid bilayer-functionalized iron oxide nanoparticles disrupt early development of freshwater snail Biomphalaria glabrata (Say, 1818)

Iron oxide nanoparticles (IONPs) have been indicated for the control of parasites and intermediate hosts, as well as applications in several sectors of nanomedicine. However, knowledge regarding its toxicity, mechanisms of action and the role of functionalization in gastropods that act as intermediate hosts of neglected disease parasites is still scarce. The present study aimed to evaluate the toxicity of lauric acid bilayer-functionalized IONPs (LA-IONPs), lauric acid isolated (LA) and iron ions in embryos and newly-hatched Biomphalaria glabrata. The snails were exposed to different concentrations of IONPs, LA and iron ions (1.0–97.65 mg L-1) during 144 h (embryos) and 96 h (newly-hatched) and multiple parameters were analyzed, such as mortality, hatching rate, developmental delay, and morphological changes. The results showed that both iron forms (LA-IONPs and iron ions) and LA promoted mortality, hatching inhibition and morphological changes in snail embryos in a concentration-dependent patterns. Embryos also showed iron bioaccumulation after exposure to both iron forms. High toxicity was observed in newly-hatched snails compared to embryos, indicating the protective role of ovigerous masses during the early developmental stages. LA induced high developmental toxicity compared to LA-IONPs and iron ions. Results showed the molluscicide activity of LA-IONPs and isolated LA, indicating their potential use as molluscicide in the snail control program.

Biostimulation of Anaerobic Digestion Using Iron Oxide Nanoparticles (IONPs) for Increasing Biogas Production from Cattle Manure.

The effect of synthesised IONPs employing a nontoxic leaf extract of Azadirachta indica as a reducing, capping, and stabilizing agent for increasing biogas and methane output from cattle manure during anaerobic digestion (AD) was investigated in this study. Furthermore, the UV-visible spectra examination of the synthesized nanoparticles revealed a high peak at 432 nm. Using a transmission electron microscope, the average particle size of IONPs observed was 30–80 nm, with irregular, ultra-small, semi-spherical shapes that were slightly aggregated and well-distributed. IONPs had a polydisparity index (PDI) of 219 nm and a zeta potential of −27.0 mV. A set of six bio-digesters were fabricated and tested to see how varying concentrations of IONPs (9, 12, 15, 18, and 21 mg/L) influenced biogas, methane output, and effluent chemical composition from AD at mesophilic temperatures (35 ± 2 °C). With 18 mg/L IONPs, the maximum specific biogas and methane production were 136.74 L/g of volatile solids (VS) and 64.5%, respectively, compared to the control (p < 0.05), which provided only 107.09 L/g and 51.4%, respectively. Biogas and methane production increased by 27.6% and 25.4%, respectively using 18 mg/L IONPs as compared to control. In all treatments, the pH of the effluent was increased, while total volatile fatty acids, total solids, volatile solids, organic carbon content, and dehydrogenase activity decreased. Total solid degradation was highest (43.1%) in cattle manure + 18 mg/L IONPs (T5). According to the results, the IONPs enhanced the yield of biogas and methane when compared with controls.

Sustainable synthesis of microwave-assisted IONPs using Spinacia oleracea L. for control of fungal wilt by modulating the defense system in tomato plants

BackgroundChanging climate enhances the survival of pests and pathogens, which eventually affects crop yield and reduces its economic value. Novel approaches should be employed to ensure sustainable food security. Nano-based agri-chemicals provide a distinctive mechanism to increase productivity and manage phytopathogens, with minimal environmental distress. In vitro and in greenhouse studies were conducted to evaluate the potential of green-synthesized iron-oxide nanoparticles (IONPs) in suppressing wilt infection caused by Fusarium oxysporum f. sp. lycospersici, and improving tomato growth (Solanum lycopersicum) and fruit quality.ResultsVarious microwave powers (100–1000 W) were used to modulate the properties of the green-synthesized IONPs, using spinach as a starting material. The IONPs stabilized with black coffee extract were substantively characterized using X-ray diffraction analysis (XRD), Fourier-transform infrared spectroscopy, dielectric and impedance spectroscopy, X-ray photoelectron spectroscopy (XPS), scanning and transmission electron microscopy (SEM and TEM, respectively), and magnetization analysis. XRD revealed a cubic magnetite (Fe3O4) phase with super-paramagnetic nature, detected at all microwave powers. The binding energies of Fe 2p3/2 (710.9 eV) and Fe 2p1/2 (724.5 eV) of Fe3O4 NPs were confirmed using XPS analysis at a microwave power of 1000 W. Uniform, spherical/cubical-shaped particles with an average diameter of 4 nm were confirmed using SEM and TEM analysis. A significant reduction in mycelial growth and spore germination was observed upon exposure to different IONP treatments. Malformed mycelium, DNA fragmentation, alternation in the cell membrane, and ROS production in F. oxysporum indicated the anti-microbial potential of the IONPs. The particles were applied both through the root (before transplantation) and by means of foliar application (after two weeks) to the infected seedlings. IONPs significantly reduced disease severity by an average of 47.8%, resulting in increased plant growth variables after exposure to 12.5 µg/mL of IONPs. Analysis of photosynthetic pigments, phenolic compounds, and anti-oxidant enzymes in the roots and shoots showed an increasing trend after exposure to various concentrations of IONPs. Correspondingly, lycopene, vitamin C, total flavonoids, and protein content were substantially improved in tomato fruits after treatment with IONPs.ConclusionThe findings of the current investigation suggested that the synthesized IONPs display anti-fungal and nutritional properties that can help to manage Fusarium wilt disease, resulting in enhanced plant growth and fruit quality.Graphical

Phosphonate coating of commercial iron oxide nanoparticles for nanowarming cryopreserved samples.

New preservation technologies may allow for organ banking similar to blood and biomaterial banking approaches. Using cryoprotective agents (CPAs), aqueous solutions with organic components such as DMSO, propylene glycol, and added salts and sugars, organs can be used to vitrify and store organs at −140 °C. When needed, these organs can be rewarmed in a rapid and uniform manner if CPAs are supplemented with iron oxide nanoparticles (IONPs) in an applied radiofrequency field. Speed and uniformity of warming are both IONP concentration and CPA suspension dependent. Here we present a coating method of small molecule phosphonate linker (PLink) and biocompatible polymer (i.e. polyethylene glycol PEG) that tunes stability and increases the maximum allowable concentration of IONPs in CPA suspension. PLink contains a phosphonate 'anchor' for high irreversible binding to iron oxide and a carboxylic acid 'handle' for ligand attachment. PLink-PEG removes and replaces the initial coating layer of commercially available IONPs (EMG1200 (hydrophobic) and EMG308 (hydrophilic) Ferrotec, Inc., increasing colloidal stability and decreasing aggregation in both water and CPAs, (verified with dynamic light scattering) from minutes (uncoated) to up to 6 days. Heating properties of EMG1200, specific absorption rate (SAR), measured using an applied field of 360 kHz and 20 kA m−1, increased from 20 to 180 W per g Fe with increasing PLink-PEG5000. PEG replacing the initially hydrophobic coating decreased aggregation in water and CPA, consistent with earlier studies on heating performance. Furthermore, although the size is minimized at 0.20 mol PEG per g Fe, heating is not maximized until concentrations above 0.43 mol PEG per g Fe on EMG1200. SAR on hydrophilic EMG308 was preserved at 400 W per g Fe regardless of the amount of PLink added to the core. Herein concentrations of IONP in VS55 (common CPA) significantly above our previous capabilities, sIONP at 10 mg Fe per mL, was reached, 25 mg Fe per mL of 308-PEG5000 and 60 mg Fe per mL of 1200-PEG5000, approaching stock EMG308 in water, 60 mg Fe per mL. Furthermore, at these concentrations cryopreserved Human dermal fibroblast cells were successfully nanowarmed (at applied fields described above), with higher viability as compared to convective rewarming in a water bath and heating rate close to 200 °C min−1, 2.5 times faster than our current system. Using PLink as the coating method allowed for higher concentrations of IONPs to be successfully suspended in CPA without affecting the heating ability. Additionally, the model ligand, PEG, allowed for increased stability over time in nanowarming experiments.

Stability Analysis of Fe3O4‐OA‐MWCNT Nanocomposite‐Based Nanofluid

The aim of this project is to fabricate a highly stable Fe3O4‐OA‐MWCNT composite and its colloidal suspension in polyalphaolefin (PAO) base fluid for heat transfer applications. The nanocomposite was produced in three mass ratios (0.5 : 1, 1 : 1, and 1.5 : 1) to explore the concentration effects on the stability of the nanocomposite. XRD analysis and FTIR spectroscopy were conducted to inspect the phase and composition of the synthesized nanocomposite. The crystallite sizes (3.75 nm, 7.74 nm, and 7.52 nm) and dislocation densities of each composite were calculated, and it was revealed that the samples with high concentration of iron oxide nanoparticles showed small defects in their lattices. Dispersion stability of ferrofluids was also examined by natural deposition method for 365 days. The ferrofluids displayed high stability for more than one year with no sign of sedimentation. Thermal conductivity of the nanofluid was also measured via. A transient plane source method and a linear trend with slight deviation were observed.

Effects of Biologically Synthesized Iron Oxide Nanoparticles on Rhizospheric Microorganisms Associated with Tomato (Solanum lycopersicum L.)

In this study, the effect of iron oxide nanoparticles on soil rhizospheric microbial communities of tomato was investigated. Iron oxide nanoparticles were biologically synthesized using plant extract from Azadirachta indica, and characterized using a UV-VIS spectrophotometer. Varying concentrations (25, 50, 75, or 100 %) of biosynthesized iron oxide nanoparticles or precursor solution was rhizoinjected into soils in which tomato plants were grown. Plate count method was used to analyse population size and community structure of test subjects. Quantitative analysis of the bacterial and fungal community was determined and diversity indicies were calculated. The results obtained from the analysis revealed that the addition of iron oxide nanoparticles to the soil changed bacterial and fungal community with respect to the control. Also, the bacterial and fungal abundance were changed. Some tolerant microorganisms such as Micrococcus, Stapylococcus, Aspergillus, Trichoderma and Penicillium could withstand high concentrations of iron oxide nanoparticles. Shannon diversity indices showed that there was difference in the diverisity of each concentration of iron oxide nanoparticles for both fungal and bacterial communties. The study's findings showed that high concentration of iron oxide nanoparticles in the soil had adverse effect on both the tomato and the microorganisms associated with the root of the tomato. Further study needs to be conducted to ascertain the magnitude of impact iron oxide nanoparticles will have on plants and rhizosphere microbiome.

In-vitro evaluation of physiological changes caused by iron oxide nanoparticles in Solanum villosum

ABSTRACT Application of iron nanoparticles as iron fertilizer can be a functional strategy to manage iron deficiency in agricultural crops. However, the toxicity of iron should not be ignored and its proper concentrations should be determined. The effect of iron nanoparticles and Fe (II) ethylenediaminetetraacetate (FeEDTA) on iron accumulation, photosynthetic pigments, soluble carbohydrate and protein, antioxidant enzymes activity, and lignification were compared in vitro in Solanum villosum. Culture media were prepared in two ways: 1) including 628 mg L−1 FeEDTA as control, and 2) different concentrations of iron oxide nanoparticles (628 and 730 mg L−1 in pH of 5.2, and 628 and 830 mg L−1 in pH of 5.9). Seeds were germinated on culture media and biochemical parameters were measured at the end of the fourth week. The iron content, amount of chlorophyll, soluble carbohydrate and protein in leaves and stems of the plants grown using the nanoparticle concentration of 628 mg L−1 decreased compared to the control, but the same parameters increased in concentrations of 730 and 830 mg L−1. Iron nanoparticles decreased catalase and ascorbate peroxidase activities. Guaiacol peroxidase activity and carotenoid content showed no significant difference between FeEDTA and nanoparticle treatments. Leaf and stem lignin levels did not differ from control (FeEDTA). FeEDTA can be replaced by optimum concentrations of iron nanoparticle under in vitro conditions. In vivo experiments are needed to generalize these results for field-grown plants, because iron nanoparticles have some advantage over iron chelate, which is highly dependent on soil conditions. Abbreviations: APX: ascorbate peroxidase; CAT: catalase; FeEDTA: Fe (II) ethylenediaminetetraacetate; GP: guaiacol peroxidase; MS: Murashige and Skoog medium

The study of TSS reduction in industrial wastewater by nano-coagulants

The main objective of this study is to investigate the effect of coagulants and nano-coagulants in different concentrations to reduce the TSS of industrial wastewater using the Taguchi statistical method. Two chemicals, iron chloride and aluminum sulfate, and two nanoparticles of iron oxide and alumina, were used as coagulants within the wastewater flocculation process at Beshel industrial complex. A L16 orthogonal array with the four factors of control at four levels each and two interactions was employed for optimization of coagulants and nano-coagulants concentration to reduce total suspended solids (TSS) in industrial wastewater. The concentration of iron chloride 1.5 g/l, concentration of iron oxide nanoparticles 1.5 g/l, concentration of aluminum sulfate 1.5 g/l and concentration of alumina nanoparticles 1.5 g/l was the optimal conditions to reduce the TSS of industrial wastewater.

Highly Efficient Iron Oxide Nanoparticles Immobilized on Cellulose Nanofibril Aerogels for Arsenic Removal from Water.

The application and optimal operation of nanoparticle adsorbents in fixed-bed columns or industrial-scale water treatment applications are limited. This limitation is generally due to the tendency of nanoparticles to aggregate, the use of non-sustainable and inefficient polymeric resins as supporting materials in fixed-bed columns, or low adsorption capacity. In this study, magnesium-doped amorphous iron oxide nanoparticles (IONPs) were synthesized and immobilized on the surface of cellulose nanofibrils (CNFs) within a lightweight porous aerogel for arsenic removal from water. The IONPs had a specific surface area of 165 m2 g−1. The IONP-containing CNF aerogels were stable in water and under constant agitation due to the induced crosslinking using an epichlorohydrin crosslinker. The adsorption kinetics showed that both As(III) and As(V) adsorption followed a pseudo second-order kinetic model, and the equilibrium adsorption isotherm was best fitted using the Langmuir model. The maximum adsorption capacities of CNF-IONP aerogel for As(III) and As(V) were 48 and 91 mg As g-IONP−1, respectively. The optimum IONP concentration in the aerogel was 12.5 wt.%, which resulted in a maximum arsenic removal, minimal mass loss, and negligible leaching of iron into water.

Magnetic separation of micron-sized particles: process study and regression modelling using moving least squares and multivariable power least squares method

The presence of fine particles has reduced the quality of water resources. Unfortunately, the fine particles normally take a longer time to be removed. Realizing this, the present study aims to employ a magnetic adsorbent to assist a fast separation of the fine particles from their solution. Here, micron-sized SiO2 particles were used as the model fine particles; meanwhile, the magnetic adsorbent was designed by functionalizing iron oxide nanoparticles (IONPs) with chitosan. A process study was done based on the effects of medium pH, IONPs concentration, and contact time. Results implied that both bare IONPs and chitosan functionalized-IONPs (denoted as CF-IONPs) were best in removing SiO2 at pH 4 owing to the electrostatic attraction force. However, as compared to the bare counterpart, CF-IONPs is more suitable for large scale application as it is effective in a wider pH range, requires lesser adsorbent dosage and shorter adsorption time. Despite that, removal of SiO2 using both types of adsorbents were found to follow pseudo-second order kinetic. The obtained data was modeled using either the moving least squares (MLS) or multivariable power least squares (MPLS) method. In particular, the indices of each parameter obtained from the MPLS-generated equation indicated that the most dominant parameter that governing the SiO2 removal by bare IONPs is medium pH, while the one for CF-IONPs is contact time.

Minimum Inhibitory Concentration of Iron Oxide Nanoparticles with Hydrogen Peroxide against Endodontic Enterococcus faecalis

Aims: This study aimed to assess the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) effect of iron oxide nanoparticles (IONPs) in combination with hydrogen peroxide (H2O2) by evaluating its effect against endodontic Enterococcus Faecalis ( E. faecalis) in the planktonic model. Materials and Methods: the tested irrigants groups (IONPs, 3% H2O2, 5.25% NaOCl, IONPs+H2O2) were serially diluted in brain heart infusion broth, and 100 μl of standard microbial suspensions of E. faecalis were added. The results were obtained based on turbidity and growth on selective enterococcus agar plates. Results: IONPs+H2O2 showed to be more effective against E. faecalis than IONPs, 3% H2O2 and 5.25% NaOCl as lower dilutions were required to inhibit the growth of the bacteria. NaOCl exhibited an antibacterial effect equivalent to that of H2O2, while IONPs exhibited a stimulatory growth at the high tested concentration. Conclusions: IONPs+H2O2 showing promissory antibacterial properties as an intracanal irrigating solution to control E. faecalis from the infected root canal.

Physicochemical characteristics of polysulfone nanofiber membranes with iron oxide nanoparticles via electrospinning

AbstractHydrophilicity is one of the ideal properties for water treatment membrane. In addition, higher surface area with a porous structure results in extraordinary permeability and selectivity, thus making it a good candidate for water permeability. This study used iron oxide nanoparticles (IONPs) as polysulfone (PSf) membrane nanofillers to enhance the physicochemical properties. Nanofiber membranes were characterized in terms of morphology, wettability, and pure water permeability (PWP). The concentration of IONPs was varied from 0.5% to 2.0% (w/v). From the scanning electron microscope images, all produced membranes were smooth and contain bead‐free nanofibers. The produced nanofiber membrane with 1% (w/v) of IONPs concentration was found to be optimum since it had the highest porosity, lower contact angle, and desirable fiber thickness and diameter. In order to find the best PSf solution concentration, the loadings of PSf were varied from 18% to 27% (w/v). The PSf/IONPs with 25% (w/v) provided the best results in terms of fiber thickness and diameter, porosity and morphology. The contact angle also confirmed that in the presence of IONPs, the nanofiber structure produced with 25% PSf was less hydrophobic and had the highest PWP (70.27 L/m2 h bar).

Influence of the Aspect Ratio of Iron Oxide Nanorods on Hysteresis-Loss-Mediated Magnetic Hyperthermia.

Owing to the problems associated with conventional cancer treatment methods, magnetic hyperthermia-based cancer therapy has gained importance recently. Achieving the desired heating effect at the site of the tumor with a minimal concentration of iron oxide nanoparticles (IONPs) and a safer field is necessary to explore the advantages of hyperthermia. For one to address this challenge, biocompatible IONPs with a desirable magnetic response at a tolerable field are necessary. In this work, magnetic shape anisotropy of iron oxide nanorods (NR) of different lengths (70, 115, 170, and 210 nm) with different aspect ratios ranging from 1.55 to 3.2 was explored to achieve higher hysteresis loss, in turn leading to better hyperthermia efficiency. The magnetic properties of the NRs with respect to the applied field were studied using micromagnetic simulation. Even though the nanorods with high aspect ratio showed a higher hysteresis loss of 69485 J/m3 at 2000 Oe, the field required to attain it was high and well beyond the safety limit. From nanorods of various aspect ratios, the nanorod with a lower aspect ratio of 1.55 and a length of 70 nm exhibited a better hysteresis loss and specific absorption rate (SAR) value of 4214 W g-1 was achieved at a frequency and alternating magnetic field of 400 kHz and 800 Oe, respectively. The PEGylated GO-Nanorod of 70 nm exhibited excellent antitumor efficacy in 4T1 tumor model mice by obstructing the tumor progression within a safer dosage and field.

Humidification Effect on the Performance and Emissions of (DI) Diesel Engine Running on Diesel Fuel with Biodiesel Blended Nano Additives

The effect of humidification of the air on the performance of a compression ignition engine operating on diesel, biodiesel with nano additives was investigated. The experiment was carried out on a single-cylinder, four-stroke, naturally aspirated water-cooled, direct injection Ricardo (E6/US) diesel engine at a constant speed of 1800 rpm, and varying loads. A mixture of Biodiesel (waste cooking oil) and diesel fuel by four ratios (B5, B10, B15, and B20) was used in the experiment. Besides, five concentrations of Iron oxide nanoparticles (Fe2O3, with particle size 20 nm) as fuel-additives were prepared (10 ppm, 30 ppm, 50 ppm, 70 ppm, and 100 ppm), and added to the test fuels (Bio-Diesel). Taguchi Method by DOE was used for the optimization in this investigation. The results of Taguchi Method experiments identified the biodiesel (B20), nano additive (100 ppm), relative humidity (65%). The experimental results manifested that BTE improved by 17.62% and BSFC decreased by 12.72%, while NOx and PM reduced by 8.45%, 24.17%, respectively.

Investigating the effect of coagulants and nano-coagulants to reduce biological oxygen demands in industrial wastewater

One of the environmental consequences of industrial estates is the creation of environmental pollution, including the production of industrial wastewater. In this research, the main objective is to investigate the effect of coagulants and nano-coagulants in different concentrations to reduce the BOD of industrial wastewater using the Taguchi statistical method. Two chemicals, ferric chloride and aluminum sulphate, and two nanoparticles of iron oxide and alumina, were used as coagulants in the wastewater flocculation process at Beshel industrial complex. A L16 orthogonal array with the four factors of control at four levels each and two interactions was employed for optimization of coagulants and nano-coagulants concentration to reduce biological oxygen demands (BOD) in industrial wastewater. The optimal conditions were obtained concentration of ferric chloride 0.8 g/l, concentration of iron oxide nanoparticles 3 g/l, concentration of aluminum sulphate 1 g/l and concentration of alumina nanoparticles 1.5 g/l to achieve the highest percentage of BOD reduction.

Facile Green Synthesis of Iron Oxide Nanoparticles Using Phoenix dactylifera L. Seed Extract and Their Antibacterial Applications

Aim: The synthesis methods of iron oxide nanoparticles (IONPs) have got great attention in recent years, due to their variety in physicochemical properties and applications. This study aimed for the green synthesis of the IONPs using an aqueous extract of P. dactylifera L. seeds for its antibacterial applications.&#x0D; Methodology: IONPs were prepared in an aqueous seed extract of P. dactylifera L. The physicochemical characterisations were performed with transmission electron microscopy (TEM) to study the shape of obtained nanoparticle, energy-dispersive spectroscopy (EDS) for elemental confirmation of iron and oxygen, dynamic light scattering (DLS) for particles size measurement, vibrating sample magnetometer (VSM) for saturation magnetisation, Infrared spectroscopy (FTIR) for chemical confirmation of function groups and X-ray diffraction (XRD) for crystalline nature. The polyphenols contents were also determined; we suggest that the presence of phenolic compounds is the key element involved in the formation and the stabilisation of IONPs. Antibacterial activity was determined using disc diffusion assay and minimum inhibitory concentration (MIC) of the IONPs were determined by broth dilution assay.&#x0D; Results: The biosynthesised IONPs showed crystalline magnetite spherical morphologies with average diameter 30 nm. Discs with all the concentrations of IONPs tested in this study (10 - 100 ug.mL-1) showed antibacterial activity against bacterial strains tested (Staphylococcus epidermidis, Klebsiella pneumoniae and Pseudomonas aeruginosa). MIC values were found to be 30 ug.mL-1, 50 ug.mL-1 and 60 ug.mL-1 for S. epidermidis, K. pneumoniae and P. aeruginosa respectively.&#x0D; Conclusion: The biosynthesis of IONPs has provided, reliable, safe, simple and eco-friendly method. Hence, this study has focused on biological method of synthesis of IONPs. The IONPs synthesized in this study can be used as the therapeutic agents against bacteria.

Stability and Transportation of Iron Oxide Nanoparticles in Subsurface Water and Soil

Iron oxide nanoparticles are utilised in a broad range of applications (magnetic data storage, biosensing, drug delivery, treatment and remediation of contaminated soil and groundwater); causing noticeable quantities of iron oxide nanoparticles to be released into the environment. In this study, aggregation and stability of iron oxide nanoparticles in water were investigated within the range of pH 3 – pH 9 at a constant concentration of iron oxide nanoparticles and humic acid. The pH of the solution was selected to be continuously monitored at pH 7 to investigate the transportation and deposition behaviour of iron oxide nanoparticles in porous media at different velocities. It was found that iron oxide nanoparticles were aggregated and settle down as settled particles at low pH (pH 3 – pH 5). Iron oxide nanoparticles were stable, mobile and transported at a high pH, which is the pH range of natural water (pH 6.5 – pH 8.5). Iron oxide nanoparticles were strongly attached to the silica sand at natural water velocity in porous media, which is 2.93 ml min-1. Overall, the presence of humic acid in aquifers and porous media, the pH range of natural water (pH 6.5 – pH 8.5) and the slow speed (2.93 ml min-1) of water are expected to be a key factor that enhances the stability and mobility of iron oxide nanoparticles in natural water and porous media.

Investigation of Thermal Effects of Radiofrequency Ablation Mediated with Iron Oxide Nanoparticles Dispersed in Agarose and Chitosan Solvents

Thermal ablation (TA) is known as an alternative therapy to surgery to treat tumors. However, TA-based therapy requires advanced approaches in order to prevent causing damage to healthy tissue around the tumor and selectively target the desired area. Nanoparticles are considered as a promising tool in biomedicine to fulfill these requirements. This study was carried out in order to analyze the effect of iron oxide nanoparticles on the temperature increment during radiofrequency ablation therapy of porcine liver. In addition, this research aimed to experimentally evaluate the impact of two solvents such as agarose and chitosan on the temperature change, when magnetic nanoparticles were dispersed in them. The iron oxide nanoparticles were synthesized by the solvothermal method demonstrating the magnetic properties by acting to the external magnetic field. To increase the local heat superparamagnetic nanoparticles (iron oxide magnetic nanoparticle (IONPs)) of the average size of 20 nm in size and the concentrations from 1 to 10 mg/mL of MNPs with a step size of 1 mg/mL were tested in 10 replicates for each concentration and solvent. Moreover, the temperature changes for dry liver, and 0 mg/mL concentration was checked for calibration and reference purposes. As a sensing system, advanced 16-FBG optical fiber sensors connected to an interrogator were employed allowing the temperature change to be monitored accurately in real time. A maximum temperature of about 142 °C was recorded by a 5 mg/mL concentration of iron oxide nanoparticles dispersed in the agarose solvent.

Evaluation of potential immunotoxic effects of iron oxide nanoparticles (IONPs) on antioxidant capacity, immune responses and tissue bioaccumulation in common carp (Cyprinus carpio)

Nanoparticles can be toxic and put human and animals' life at risk. The present work was carried out to evaluate the possible immunosuppressive effects of water borne iron oxide nanoparticles (IONPs) and the FeCl3 on immune components of common carp (Cyprinus carpio). Fish were exposed to a series of chronic levels of 25%, 50%, and 75% of IONPs LC50 96 h concentration (referred to as control, NP1, NP2, and NP3 respectively) or FeCl3 (same concentrations as IONPs referred to as S1, S2, and S3 respectively) for 21 days. Results revealed alterations in blood parameters, where IONPs significantly decreased number of white blood cells at all concentrations. Glucose and cortisol increased in all exposed fish after 21 days, suggesting activation of the maintenance mechanism cascade against a chronic stressor. IONPs or FeCl3 significantly accumulated in liver tissue of exposed fish. Immune responses were remarkably decreased in serum and skin mucus in response to IONPs or FeCl3. These components were at lowest levels in fish exposed to the highest concentrations of IONPs and FeCl3. The findings of the present study suggested that IONPs is accumulated in fish liver and intestine, where they may exhibit immunotoxicity.

Intravesical In Situ Immunostimulatory Gel for Triple Therapy of Bladder Cancer.

Bladder cancer displays multiple biological features aided in drug resistance; therefore, single therapy fails to induce complete tumor regression. To address this issue, various kinds of cell death of cancer cells as well as restoring tumor immune microenvironment need to be taken into consideration. Here, we introduce a gel system termed AuNRs&IONs@Gel, which target-delivers a combination of photothermal, ferroptotic, and immune therapy through intravesical instillation. AuNRs&IONs@Gel consists of a gel delivery platform, embedded gold nanorods (AuNRs), and iron oxide nanoparticles (IONs). The targeted delivery gel platform provides dextran aldehyde-selective adhesion with cancer collagen. In this condition, photothermal therapy can be performed by gold nanorods (AuNRs) under imaging-guided near-infrared radiation. Local high concentrations of IONs can be absorbed by cancer cell to induce ferroptosis. Moreover, tumor-associated macrophages which often display an immune-suppressive M2-like phenotype will be repolarized by IONs into the antitumor M1-like phenotype, exerting a direct antitumor effect and professional antigen presentation of dead cancer cells. This process triggers a potent immune response of innate and adapt immunities to protect tumor rechallenge in long terms. Our triple-therapy strategy employs FDA-approved nanoparticles to inhibit bladder cancer which may possess great potential for clinical translation.