Bioclimate and natural vegetation have a long - term relationship that identify the potential vegetation distribution at different areas. For that reason, bioclimatic classification system was applied to the territory of Ba and Kone river basin, Vietnam. The precipitation and temperature dataset of Ba and Kone river basin was collected from 17 climate, hydrology, rain gauge stations which allowed to create a bioclimatic map at a scale of 1:250.000. Three bioclimatic factors of thermal-moisture basic conditions such as annual temperature (TN), annual rainfall (RN), length of dry season (n) are selected as criteria system of Ba and Kone river basin’s bioclimate. In order to describe the relationships between bioclimatic variables and zonal vegetation units, the resulting map presented 12 bioclimatic units corresponding distribution of vegetation from low to high altitudes. By building bioclimatology map in Ba and Kone river basin, the government can develop sustainable agro forestry in Central Highlands and South Central Coast of Vietnam.