Abstract
BackgroundScylla paramamosain is one of the commercially crucial marine crustaceans belonging to the genus Scylla, which is commonly distributed along the coasts of China, Vietnam, and Japan. Genomic and transcriptomic data are scarce for the mud crab. Light intensity is one of the ecological factors that affect S. paramamosain during indoor overwintering. To understand the energy metabolism mechanism adapted to light intensity, we analyzed the transcriptome of S. paramamosain hepatopancreas in response to different light intensities (0, 1.43, 40.31 μmol·m− 2·s− 1).ResultsA total of 5052 differentially expressed genes were identified in low light group (LL group, 3104 genes were up-regulated and 1948 genes were down-regulated). A total of 7403 differentially expressed genes were identified in high light group (HL group, 5262 genes were up-regulated and 2141 genes were down-regulated). S. paramamosain adapts to different light intensity environments through the regulation of amino acids, fatty acids, carbon and energy metabolism. Different light intensities had a strong impact on the energy generation of S. paramamosain by influencing oxygen consumption rate, aerobic respiration, glycolysis/gluconeogenesis pathway, the citrate cycle (TCA cycle) and fatty acid degradation.ConclusionLow light is more conducive to the survival of S. paramamosain, which needs to produce and consume relatively less energy to sustain physiological activities. In contrast, S. paramamosain produced more energy to adapt to the pressure of high light intensities. The findings of the study add to the knowledge of regulatory mechanisms related to S. paramamosain metabolism under different light intensities.
Highlights
Scylla paramamosain is one of the commercially crucial marine crustaceans belonging to the genus Scylla, which is commonly distributed along the coasts of China, Vietnam, and Japan
Expressed genes (DEGs) in the hepatopancreas of S. paramamosain A total of nine samples were used in this analysis
In this study, we used RNA-sequencing to construct gene expression profiles related to metabolism mechanisms in S. paramamosain
Summary
Scylla paramamosain is one of the commercially crucial marine crustaceans belonging to the genus Scylla, which is commonly distributed along the coasts of China, Vietnam, and Japan. Light intensity is one of the ecological factors that affect S. paramamosain during indoor overwintering. Overwintering is an important life stage for aquatic animals living in some areas to adapt to cold winter conditions [1]. There are many factors affecting the environment of aquatic animals during overwintering, such as salinity, temperature, food availability, and light. Light is one of the most variable water quality factors that directly or indirectly affects behavior [4, 5], survival and growth [6, 7], skin color [8, 9], digestion and immunity [10], fatty acid composition [11], and oxidative stress [12] in aquatic animals. Appropriate light conditions can improve the feeding rate of shrimp and crab larvae and promote growth and metamorphosis [15]. This phenomenon is evident in the early developmental stages of shrimp and crab larvae
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