Aboveground biomass (AGB) is a critical indicator for monitoring the crop growth status and predicting yields. UAV remote sensing technology offers an efficient and non-destructive method for collecting crop information in small-scale agricultural fields. High-resolution hyperspectral images provide abundant spectral-textural information, but whether they can enhance the accuracy of crop biomass estimations remains subject to further investigation. This study evaluates the predictability of winter canola AGB by integrating the narrowband spectra and texture features from UAV hyperspectral images. Specifically, narrowband spectra and vegetation indices were extracted from the hyperspectral images. The Gray Level Co-occurrence Matrix (GLCM) method was employed to compute texture indices. Correlation analysis and autocorrelation analysis were utilized to determine the final spectral feature scheme, texture feature scheme, and spectral-texture feature scheme. Subsequently, machine learning algorithms were applied to develop estimation models for winter canola biomass. The results indicate: (1) For spectra features, narrow-bands at 450~510 nm, 680~738 nm, 910~940 nm wavelength, as well as vegetation indices containing red-edge narrow-bands, showed outstanding performance with correlation coefficients ranging from 0.49 to 0.65; For texture features, narrow-band texture parameters CON, DIS, ENT, ASM, and vegetation index texture parameter COR demonstrated significant performance, with correlation coefficients between 0.65 and 0.72; (2) The Adaboost model using the spectra-texture feature scheme exhibited the best performance in estimating winter canola biomass (R2 = 0.91; RMSE = 1710.79 kg/ha; NRMSE = 19.88%); (3) The combined use of narrowband spectra and texture feature significantly improved the estimation accuracy of winter canola biomass. Compared to the spectra feature scheme, the model’s R2 increased by 11.2%, RMSE decreased by 29%, and NRMSE reduced by 17%. These findings provide a reference for studies on UAV hyperspectral remote sensing monitoring of crop growth status.