Clinical Signs Of Disease Research Articles

α-Casozepine supplementation shows no effect on foals' growth and cortisol levels before and after weaning.

The goal of this study was to determine whether oral administration of α-casozepine (Zylkene) supplementation decreases the risk of disease in foals, as measured through salivary cortisol levels, temperature, clinical signs of disease, and body weight before and after weaning. 20 Thoroughbred foals were observed over 10 weeks, beginning 14 days prior to the time of weaning. Experimental foals were randomly selected and given α-casozepine daily for 10 weeks, while the controls received a placebo with the same frequency as the experimental group. The average daily height and weight gain were calculated to examine the effects of α-casozepine before and after weaning. Salivary cortisol levels were also compared before versus after weaning. There was no statistically significant difference in average daily height/weight gain or cortisol levels between the α-casozepine group and the control before and after weaning. However, the α-casozepine group had numerically less of a decline in height and growth compared to the control. This suggests that with further study Zylkene may decrease losses in foal growth rate. The weaning process is one of the largest stressors in a young horse's life. This abrupt process can contribute to an increased risk of disease and even morbidity. Other major stressors including separation from the mare, diet change, and new social groups can also lead to animal health and welfare concerns, as well as economic losses in weanling foals. However, further study is required to determine the benefits of α-casozepine on equine stress and health.

Evidence of Active Orbivirus Transmission in 2016 in Kansas and Nebraska.

Retrospective serological and case diagnostic data of endemic bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) provide evidence of viral transmission among livestock and wildlife from 2016 in Kansas and Nebraska. Serological testing of mature cattle in nine distinct regional zones of Kansas revealed 76% to 100% had detectable antibodies to BTV and/or EHDV. Specimens tested in the Kansas Veterinary Diagnostic Laboratory (55 submissions) were 51% test positive for antibodies to BTV and/or EHDV. Specimens tested in the Nebraska Veterinary Diagnostic Center (283 submissions) were 25% test positive for antibodies to BTV and/or EHDV. Low disease incidence in white-tailed deer and other susceptible wild ungulates was observed during 2016. However, there were no confirmed reports of disease in livestock in either state. The reasons for emergence of significant clinical disease in livestock and wildlife populations remain undefined.

Development of a bovine liver biopsy training model

In veterinary medicine, liver biopsy is a standard diagnostic procedure for studying liver disease in animal species, including cows. Some common indications for liver biopsy in cattle include clinical signs of liver disease (e.g., anorexia, jaundice, lethargy), unexplained weight loss, and monitoring of health and nutritional status. This technique comes with certain risks, such as haemorrhage, infections, and lesions of other organs, so it shouldonly be carried out by a trained and experienced veterinarian. The Clinical Skills Laboratory is a structured teaching laboratory that provides hands-on experience to veterinary medicine students in a safe, controlled environment. The laboratory focuses on developing practical competencies in various veterinary procedures. This paper presents the development of a model for performing liver biopsy in cattle and discusses the importance of the skills lab in veterinary medicine learning. This model enables students to learn how to perform a liver biopsy in cattle and the basic rules of sampling and fixation of the specimen for histopathological examination. It is accompanied by detailed work instructions and illustrations to assist the student in performing the procedure. This kind of laboratory is indispensable in veterinary medical training, as it helps students strengthen their skills and confidence, preparing them for a successful career in veterinary medicine. In addition, it allows for the execution of techniques that would not be practised on a live animal during classes.

Clinical, radiographic and histological findings of seven teeth from two California sea lions (Zalophus californianus) housed under professional care.

Seven teeth extracted from two adult California sea lions (Zalophus californianus) due to pulp exposure and/or to gain access to the mandibular canine teeth were histologically evaluated, and the findings were compared with clinical and radiographic findings. Three teeth were diagnosed with pulp exposure, and two of these showed no radiographic signs of endodontic disease and were histologically vital with prominent coronal pulpitis and a pulp polyp. Another tooth with pulp exposure was showing clinical and radiographic signs of endodontic disease and was histologically confirmed with pulp necrosis. A discoloured incisor tooth was showing radiographic signs of endodontic disease and was also histologically non-vital. Two clinically and radiographically healthy mandibular first premolar teeth and one second incisor tooth had no evidence of pulpitis or pulp necrosis but had pulp canal obliteration. Regular clinical and radiographic follow-up for 5 months to 3 years after the procedures confirmed uneventful healing of the extraction sites, despite initial flap's dehiscence. Although extractions of affected teeth in California sea lions are considered the most practical and beneficial therapy, these are associated with the risks of extensive trauma and anaesthesia and the need to perform these surgical procedures on-site under variable conditions. As California sea lions can be trained to allow conscious dental radiographic re-checks, monitoring teeth with clinical signs of pulp polyp formation and without radiographic signs of endodontic disease warrant further evaluation/reconsideration from previous recommendations. Endodontic treatment of abscessed teeth in California sea lions is reportedly unsuccessful and is discouraged. However, vital pulpectomy could be an alternative treatment to extraction in teeth with pulp polyps as it was found to be highly successful in humans, but the possibility of endodontic failure and need for further treatments should be weighted in the treatment choice.

Swollen Disc in Children : Diagnostic Approach

Swollen disc is a clinical sign of eye disease, neurological or systemic. It happened because of the increase in intracranial. Some of the conditions that cause swollen disc are optic neuritis, optic neuropathy, retinal vein occlusion, papilloedema, or pseudo-disc swelling (e.g drusen). In children, the incidence of swollen discs differs from that in adults. Diagnosis can be made using OCT (Optical Coherence Tomography), ocular ultrasound, VEP (Visual Evoked Potential) and MRI (Magnetic Resonance Imaging) of the orbit and head

An experimental game to assess hunter’s participation in zoonotic diseases surveillance

BackgroundStrengthening the surveillance of zoonotic diseases emergence in the wild meat value chains is a critical component of the prevention of future health crises. Community hunters could act as first-line observers in zoonotic pathogens surveillance systems in wildlife, by reporting early signs of the possible presence of a disease in the game animals they observe and manipulate on a regular basis.MethodsAn experimental game was developed and implemented in a forested area of Gabon, in central Africa. Our objective was to improve our understanding of community hunters' decision-making when finding signs of zoonotic diseases in game animals: would they report or dissimulate these findings to a health agency? 88 hunters, divided into 9 groups of 5 to 13 participants, participated in the game, which was run over 21 rounds. In each round the players participated in a simulated hunting trip during which they had a chance of capturing a wild animal displaying clinical signs of a zoonotic disease. When signs were visible, players had to decide whether to sell/consume the animal or to report it. The last option implied a lowered revenue from the hunt but an increased probability of early detection of zoonotic diseases with benefits for the entire group of hunters.ResultsThe results showed that false alerts—i.e. a suspect case not caused by a zoonotic disease—led to a decrease in the number of reports in the next round (Odds Ratio [OR]: 0.46, 95% Confidence Interval [CI]: 0.36–0.8, p < 0.01). Hunters who had an agricultural activity in addition to hunting reported suspect cases more often than others (OR: 2.05, 95% CI: 1.09–3.88, p < 0.03). The number of suspect case reports increased with the rank of the game round (Incremental OR: 1.11, CI: 1.06–1.17, p < 0.01) suggesting an increase in participants’ inclination to report throughout the game.ConclusionUsing experimental games presents an added value for improving the understanding of people’s decisions to participate in health surveillance systems.

Loss of expression of the glutamate dehydrogenase (gdh) of Streptococcus suis serotype 2 compromises growth and pathogenicity

Streptococcus suis serotype 2 is a zoonotic agent that causes substantial economic losses to the swine industry and threatens human public health. Factors that contribute to its ability to cause disease are not yet fully understood. Glutamate dehydrogenase (GDH) is an enzyme found in living cells and plays vital roles in cellular metabolism. It has also been shown to affect pathogenic potential of certain bacteria. In this study, we constructed a S. suis serotype 2 GDH mutant (Δgdh) by insertional inactivation mediated by a homologous recombination event and confirmed loss of expression of GDH in the mutant by immunoblot and enzyme activity staining assays. Compared with the wild type (WT) strain, Δgdh displayed a different phenotype. It exhibited impaired growth in all conditions evaluated (solid and broth media, increased temperature, varying pH, and salinity) and formed cells of reduced size. Using a swine infection model, pigs inoculated with the WT strain exhibited fever, specific signs of disease, and lesions, and the strain could be re-isolated from the brain, lung, joint fluid, and blood samples collected from the infected pigs. Pigs inoculated with the Δgdh strain did not exhibit any clinical signs of disease nor histologic lesions, and the strain could not be re-isolated from any of the tissues nor body fluid sampled. The Δgdh also showed a decreased level of survival in pig blood. Taken together, these results suggest that the gdh is important in S. suis physiology and its ability to colonize, disseminate, and cause disease.

Automated hematological cell count using sysmex XN-1000V in Testudo hermanni: Agreement with manual count

Mediterranean area represents the main habitat of Testudo hermanni. Clinical signs of disease of these tortoises are non-specific, making the hematology results crucial in revealing underlying pathological conditions. However, accurate automated identification of blood cell populations is hampered by the presence of nucleated erythrocytes (NRBC) and thrombocytes (Thr), necessitating manual methods such as counting chambers. The aim of the study was to assess the performance of the novel automated hematology analyzer Sysmex XN-1000 V, which includes a a specific channel (WNR) for counting NRBC, in accurately identify and quantify the different blood cell populations of Testudo hermanni. Additionally, its agreement with manual counts was evaluated. Fifty heparinized blood samples were initially counted using the Neubauer improved chamber and then analysed twice with Sysmex XN-1000 V. Thirteen out of 50 samples were instrumentally counted again after 48 h to assess the inter-assay precision. All WNR scattergrams were re-analysed using an ad hoc gate panel to differentiate two populations: NRBCs (weak fluorescence signal) and WBC + Thr (high fluorescence signal). Sysmex XN-1000 V demonstrated optimal intra- and inter-assay precision for NRBCs (CV 0.98% ± 1.96; 1.31% ± 2.98) and moderate precision for WBC + Thr (CV 9.24% ± 16.61; 12.69% ± 10.35). No proportional nor constant errors were observed between the methods for both the populations. The instrumental NRBC counts were consistently slightly lower, while WBC + Thr counts were slightly higher compared to manual counts. These findings suggest that Sysmex XN-1000 V can be used for analyzing cell populations in heparinized blood of Testudo hermanni. However, specific instrumental reference intervals are suggested.

Prevalence of middle ear effusion in French bulldogs without clinical signs of otic disease: A retrospective study of magnetic resonance imaging (2017-2022).

Canine middle ear effusion (MEE) is usually asymptomatic, being an incidental finding when computed tomography or magnetic resonance imaging (MRI) of the head is performed for other reasons unrelated to otic disease. The clinical relevance of the presence of material in the tympanic bulla (TB) remains uncertain, and more detail about its prevalence and appearance in MRI are required. To assess the prevalence of presence of material within the TB of French bulldogs (FB) with no clinical signs suggestive of otitis (externa, media or interna) that underwent high-field MRI for other medical reasons. Two hundred fifty-two TB of 126 FB were included in this study. Nonexperimental retrospective study in which MRI images were evaluated by a board-certified veterinary radiologist. Fifty-eight per cent of the dogs had material in the TB lumen (46% of the TB) and 59% were bilaterally affected. The signal intensity of this material related to the grey matter was variable on T1w and mainly hyperintense on T2w sequences. FB are predisposed to MEE. This is important when assessing imaging studies of TB of FB with chronic otitis externa, as high percentage of cases may have concurrent MEE. MRI findings in FB with MEE are characterised by a hyperintense signal to the grey matter on T2w in most cases and variable on T1w sequences.

Graft-versus-leukaemia immunity is retained following treatment with post-transplant cyclophosphamide alone or combined with tocilizumab in humanised mice.

Donor haematopoietic stem cell transplantation treats leukaemia by inducing graft-versus-leukaemia (GVL) immunity. However, this benefit is often mitigated by graft-versus-host disease (GVHD), which is reduced by post-transplant cyclophosphamide (PTCy) alone or combined with tocilizumab (TOC) in humanised mice. This study established a preclinical humanised mouse modelof GVL and investigated whether PTCy alone or combined with TOC impacts GVL immunity. NOD-scid-IL2Rγnull mice were injected with 2 × 107 human peripheral blood mononuclear cells (hPBMCs) on day 0 and with 1 × 106 THP-1 acute myeloid leukaemia cells on day 14. In subsequent experiments, mice were also injected with PTCy (33 mg kg-1) or Dulbecco's phosphate buffered saline (PBS) on days 3 and 4, alone or combined with TOC or control antibody (25 mg kg-1) twice weekly for 28 days. Clinical signs of disease were monitored until day 42. Mice with hPBMCs from three different donors and THP-1 cells showed similar survival, clinical score and weight loss. hCD33+ leukaemia cells wereminimal in mice reconstituted with hPBMCs from two donors but present in mice with hPBMCs from a third donor, suggesting donor-specific GVL responses. hPBMC-injected mice treated with PTCy alone or combined with TOC (PTCy + TOC) demonstrated prolonged survival compared to control mice. PTCy alone and PTCy + TOC-treated mice with hPBMCs showed minimal hepatic hCD33+ leukaemia cells, indicating sustained GVL immunity. Further, the combination of PTCy + TOC reduced histological damage in the lung and liver. Collectively, this research demonstrates that PTCy alone or combined with TOC impairs GVHD without compromising GVL immunity.

ЗАБОЛЕВАНИЕ РЕСПИРАТОРНОГО ТРАКТА У МЕЛКОГО РОГАТОГО СКОТА

In the farms of our country, including in the Krasnodar Territory, the infectious disease mancheimiosis in sheep and goats has been established, which previously did not exist. Clinical signs of respiratory system disease were found in animals on the farm. When observed, depression, fever, refusal of food, cough, respiratory disorders, shortness of breath, then mucous and purulent discharge from the nasal cavity were detected. Within 5-7 days after the appearance of clinical signs of the disease, such animals died, and individual animals died within 12-24 hours. Mastitis was observed in sick sheep and goats, while the mammary gland had a blue color. Upon autopsy of the corpses of dead animals, various pathomorphological changes in the organs of the respiratory system were found in all of them. Spotty and spotty hemorrhages were detected in the nasal cavity, hemorrhages were detected on the mucous membranes of the larynx, trachea, pleural, abdominal surface, endocardium, epicardium. In addition, fibrinous bronchopneumonia, pulmonary edema, pleurisy, accumulation of foamy fluid in the trachea and bronchi, gastritis, conjunctivitis, keratitis and other changes were detected. At the same time, an accumulation of foamy exudate from pieces of fibrin in the bronchi and trachea, an increase in bronchial and mediastinal lymph nodes, hemorrhages on the pericardium, endocardium, catarrhal and hemorrhagic inflammation of the rennet and conjunctiva of the eye were detected in the bronchi and trachea. The diagnosis was carried out comprehensively, taking into account bacteriological studies. The pathogenic species Mannheimia haemolytica was isolated from the pathomaterial of sick and dead sheep and goats. Primers were obtained in the laboratory to identify the genome of the pathogen Mannheimia haemolytica in farm animals (small cattle) during PCR diagnostics.

Differential Diagnosis of Trigger Thumb in Children

Trigger thumb is the most common hand deformity in young children. The differential diagnoses of trigger thumb are thumb-in-palm deformity, hyperextension deformity of the metacarpophalangeal joint, thumb hypoplasia and congenital stiffness of the interphalangeal joint. The knowledge of the typical clinical signs of these different diseases enables the surgeon to make the correct diagnosis, leading to the right treatment.

Surveillance of Mycoplasma agassizii in Texas tortoises (Gopherus berlandieri) for translocation with emphasis on treatment and recovery.

Texas tortoises (Gopherus berlandieri) are a Texas-state threatened species. Translocation is often suggested as a mitigation option; however, disease status and the potential for spread must be considered prior to such efforts. Mycoplasma infection of the upper respiratory tract is a concern within tortoise populations, which requires monitoring so translocation efforts do not inadvertently spread the disease. We determined and compared the prevalences of Mycoplasma agassizii in Texas tortoises from donor and recipient sites in southern Texas prior to translocation, treated Mycoplasma agassizii-infected tortoises with danofloxacin, and developed alternate Mycoplasma agassizii treatments for Texas tortoises. We collected 171 and 23 Texas tortoises from a 270-ha and a 100-ha donor site and recipient site, respectively. We began a regimen of danofloxacin (6 mg/kg body weight injected subcutaneously every other day for 30 days) for tortoises with clinical signs (N = 20). We noted an additional 10 tortoises began displaying clinical signs of upper respiratory tract disease (URTD) after translocation, so we designed a trial to test tulathromycin (5 mg/kg body weight given intramuscularly once/week for 7 weeks) or oxytetracycline (8 mg/kg body weight given subcutaneously once/day for 14 days) as Mycoplasma treatments for symptomatic tortoises. Within the donor and recipient sites, 56 (32.7%) and 8 (34.8%), respectively, had antibody titers suggestive of past exposure. Eighteen tortoises from the donor site (10.5%) and 2 from the recipient site (8.7%) displayed clinical signs (i.e., clear serous nasal discharge, conjunctivitis, and palpebral edema) consistent with Mycoplasmal URTD upon initial collection, even though all polymerase chain reaction (PCR) results were negative for active shedding of Mycoplasma agassizii. We ceased treatment after the first dose of danofloxacin due to adverse reactions, which only began to subside after 72 h from the initial dose. Neither tulathromycin or oxytetracycline caused the clinical signs of URTD to subside after a 50-day treatment period. Mycoplasma is a persistent issue facing Texas tortoises. Stressors, such as translocation, can cause Mycoplasma-seropositive tortoises to display clinical symptoms of URTD, which can abate without treatment, once the stressor subsides. Danofloxacin, the recommended treatment for Mycoplasma infection in tortoises, is too potent for Texas tortoises.

Preventive myocardial revascularization prior to abdominal aortic repair in patients without cardiac symptoms: long-term results

To evaluate the long-term influence of preoperative invasive coronary screening and preventive myocardial revascularization on mortality and cardiac complications after open surgery for abdominal aortic aneurysms (AAA). We present long-term outcomes after open surgery for AAA between 2011 and 2022. Patients without clinical or objective signs of coronary artery disease were included. In the 1st group, routine coronary angiography was performed before surgery. Prophylactic myocardial revascularization was performed in 12 cases. Long-term data on 45 patients were obtained. In the 2nd group, 53 patients underwent repair without invasive coronary screening, and data on 48 patients were obtained in this group. The median follow-up was 32 and 79 months, respectively. Kaplan-Meyer overall 48-month survival was 87.3% and 82.1%, respectively (p=0.278). In the first group, 2 patients developed angina pectoris in the same period. In the second group, we observed 2 cases of myocardial infarction and 3 cases of angina pectoris without infarction. Analysis of survival curves found no significant differences (p=0.165). In our study, invasive coronary screening and preventive myocardial revascularization in patients without clinical and objective signs of coronary artery did not improve 4-year long-term period after abdominal aortic repair. Perhaps, differences will appear after 4 years, and this requires further follow-up after coronary angiography. However, there is a tendency towards more common onsets of coronary artery disease that dictates the need for cardiac monitoring of such patients.

ORAL VACCINATION AGAINST YERSINIA RUCKERI: BIOMARKERS OF PROTEIN OXIDATION IN THE HEPATIC TISSUE OF RAINBOW TROUT (ONCORHYNCHUS MYKISS WALBAUM)

The aim of this study was to evaluate the one-month effect of oral vaccination against Yersinia ruckeri based on oxidative stress biomarkers in the hepatic tissue of rainbow trout (Oncorhynchus mykiss Walbaum). The vaccine consisted of three strains of Y. ruckeri (serotype O1) obtained from rainbow trout from different farms where fish showed clinical signs of enteric redmouth disease. The vaccine was produced at the Department of Fish Diseases, National Veterinary Research Institute in Pulawy (Poland) according to the process covered by patent no. P.428259. The concentrated vaccine was added to the fish feed. Treatment was administered three times at one-day intervals. Livers were sampled one month after immunisation. Our results show that immunisation with the anti-Yersinia vaccine did not alter the liver tissue of rainbow trout. aldehydic and ketonic derivatives of oxidatively modified proteins (OMP) were not significantly altered in the hepatic tissue of vaccinated fish prior to immunisation. One month after immunisation, the levels of ketonic derivatives of OMP in the hepatic tissue of untreated trout were reduced compared to the untreated group before immunisation. Similar to the untreated groups, the levels of ketonic derivatives of OMP in the hepatic tissue of the vaccinated group one month after immunisation were reduced compared to the vaccinated group before immunisation. A statistically non-significant decrease in the total antioxidant capacity (TAC) levels was observed between the values obtained in the untreated and vaccinated groups before vaccination and one month after vaccination. Understanding the role of oxidative stress in the tissues of vaccinated trout has important implications for understanding the complex physiological changes that occur during vaccination, and also for improving aquaculture practices to maximise tissue growth and health of vaccinated trout.

Double Aneuploidy (Trisomy 21 and XXX) in a Child with Down Syndrome Phenotype: A Case Report with Review of Literature

Abstract Background: Down syndrome is the most common and extensively studied disorder arising out of human chromosomal aberration. Several aneuploidies are associated with Down syndrome, but double trisomy (48 XXX + 21) is rarely described. Clinical Description: A 3-year-old girl presented with respiratory distress and a chronic history of feeding fatigue and failure to thrive. She had phenotypic features suggestive of Down syndrome and clinical signs of acyanotic heart disease in congestive cardiac failure. Management and Outcome: Echocardiography revealed ventricular septal defect and atrial septal defect. Karyotyping showed double trisomy (trisomy 21 and XXX). Trisomy X was an incidental finding on karyotyping with no clinical features suggestive of XXX syndrome specifically at this age. The patient was managed conservatively with decongestive therapy for congestive heart failure and oral sildenafil for pulmonary hypertension. Additional evaluations were done to identify associated anomalies, and the parents were counseled. Conclusion: Down and XXX syndromes share many clinical signs and symptoms, making karyotyping the only way to make an early diagnosis. A child having a phenotype characteristic of Down syndrome may have an unsuspected double trisomy of X chromosome along with 21 trisomy, which can only be identified by karyotyping.

Nonspecific craniovertebral spondylitis

To analyze surgical strategy for nonspecific spondylitis of the craniovertebral region (CVR) taking into account clinical features and morphological signs of disease. Eight patients with nonspecific spondylitis of CVR underwent surgery (4 women and 4 men aged 31-75 years). Three patients had pain syndrome, 5 ones - conduction disorders. Combined interventions were performed in 5 patients with neurological disorders. Of these, 3 patients underwent transoral decompression with subsequent occipitospondylodesis. In other cases, stages of surgical treatment were reverse. Four patients underwent simultaneous interventions, 1 patient - with 7-day interval. Patients with pain syndrome underwent occipitospondylodesis. In all patients, postoperative VAS score of pain syndrome decreased by 5-7 points (mean 5.5). Among 5 patients with conduction symptoms, regression of neurological disorders 1 year after surgery was achieved in 2 cases, and complete recovery was observed in 3 patients (Frankel E). In all cases, examination confirmed relief of inflammatory process and no compression of the spinal cord and medulla oblongata. One patient had a dehiscence of the wound edges of posterior pharyngeal wall, and another one had implant fracture in 3 years after surgery. Active surgical approach is reasonable for nonspecific spondylitis of CVR. Craniocervical fixation eliminates pain and risk of neurological complications following atlantoaxial instability. Conduction disorders require simultaneous transoral decompression and occipitospondylodesis in patients with nonspecific purulent craniovertebral lesions. Impaired head tilt complicates transoral stage. In this regard, it is more rational to carry out craniocervical fixation at the last stage.

Evaluation of canine tympanic membrane integrity using positive contrast computed tomography canalography.

The assessment of the integrity of the tympanic membrane (TM) can be a diagnostic challenge in patients with otitis externa and/or otitis media (OM) under an otoscopic examination. In computed tomography (CT), the TM is not always visualized. However, a positive contrast canalography using conventional radiography has been previously described to evaluate the TM integrity. This single-center study aimed to describe the positive contrast canalography technique in CT and its usefulness in identifying TM integrity in 11 dogs. Dogs with clinical signs of ear disease underwent CT canalography following a systematic protocol. The presence of contrast medium and contrast homogeneity within the tympanic cavity was assessed, confirming TM rupture when contrast medium was present in the tympanic cavity. CT canalography was performed in 16 ears, and in 7 of the 16 ears (44%), there was a contrast in the tympanic cavity, confirming TM rupture (positive CT canalography result). In two of the seven cases (29%), rupture of the TM was identified in both otoscopic and CT canalography. In the remaining five of the seven (71%) positive CT canalography results, tympanic membrane rupture was identified only by CT canalography. In conclusion, a positive contrast CT canalography is a complementary and safe technique to assess TM integrity, with a potentially higher sensitivity than otoscopic examination for the diagnosis of TM rupture in dogs.

Performance of the T-SPOT.TB test in patients with indeterminate QuantiFERON-TB Gold Plus results: proposal for an algorithm for the diagnosis of Latent Tuberculosis Infection.

Latent Tuberculosis Infection (LTBI) is a state of persistent immune response to Mycobacterium tuberculosis complex antigens without clinical, radiological and microbiological signs of active disease. Effective diagnosis and preventive treatment of LTBI are crucial for tuberculosis (TB) control, especially in high-risk groups. Currently, two main tests are used for LTBI diagnosis: the Tuberculin Skin Test (TST) and the Interferon-Gamma Release Assays (IGRA), including the QuantiFERON-TB Gold Plus (QFT-Plus) and the T-SPOT.TB. Our study evaluated the performance of the T-SPOT.TB test in patients with indeterminate QFT-Plus results, using data from the Clinical Microbiology and Virology Laboratory (M&V) of Papa Giovanni XXIII Hospital in Bergamo, Italy. Blood samples from patients tested for LTBI with QFT-Plus from January 1, 2017 to May 15, 2024 were analyzed. The QFT-Plus is the most widely used test in routine diagnostics for LTBI screening due to the availability of automated systems. Out of 20,995 samples tested with QFT-Plus, 576 (2.7%) gave indeterminate results. In all cases of indeterminate QFT-Plus results, M&V recommends performing the T-SPOT.TB test. However, of the 576 patients who obtained an indeterminate outcome, only 137 (23.8%) followed the indication. The T-SPOT.TB provided a definitive result in 87.6% of the cases, resolving 120 (80 negative and 40 positive) of 137 indeterminate QFT-Plus outcomes. Specifically, 78 of 92 cases, equal to 84.8%, were settled when the T-SPOT. TB test was performed within 30 days of the QFT-Plus. The T-SPOT.TB test has shown potential effectiveness in addressing indeterminate QFT-Plus results (84.8% resolution), indicating its possible role as a complementary diagnostic tool for LTBI. The proposed algorithm for LTBI screening is based on national and international guidelines recommending the use of the TST and/or an IGRA test for individuals at risk. However, it particularly emphasizes the use of QFT-Plus, due to its practicality and rapid execution, while recommending the addition of the T-SPOT.TB within 30 days in cases of indeterminate QFT-Plus results. Nevertheless, the conclusions should be regarded as preliminary and require confirmation through larger or controlled studies.

Salmonella typhimurium Infection in Broiler Chickens: An Overview with Special Reference to The Use of Dietary Selenium Nanoparticles for Prevention

Salmonella Typhimurium is a significant zoonotic gram-negative pathogenic bacterium that can cause foodborne illness in humans. Out of all the diseases that affect poultry, the ones caused by the Salmonella genus are the most prevalent, causing significant negative impacts on the poultry industry, including mortality, stunted growth, and decreased egg production. Moreover, broilers can serve as carriers of the bacterium without showing any clinical signs of disease, which can further contribute to the spread of infection. The use of antibiotics for the control of Salmonella Typhimurium may result in antibiotic resistance in broilers and the potential transmission of antibiotic resistance to humans via the food chain. As a result, alternative methods for controlling Salmonella Typhimurium in broilers have been explored such as the use of Selenium Nanoparticles (Se-NPs) and vaccination. Se-NPs is a form of selenium that has been shown to possess antimicrobial activity against bacteria, including Salmonella Typhimurium. Supplementation of Se-NPs in diets can reduce the prevalence and colonization of Salmonella Typhimurium in the cecum and feces of broilers. Another potential method for controlling Salmonella Typhimurium is the use of vaccines. The vaccines can be used to prevent salmonella infection including live attenuated, killed, and subunit vaccines. Therefore, the aim of this study is to highlight the Salmonella infection in broiler chickens and the importance of vaccinating broiler chickens and using Selenium Nanoparticles to protect against Salmonella infection, in order to prevent transmission of the disease to humans and safeguard the poultry industry.