The present study investigated the prevalence of coliforms, antibiotic resistant coliforms and E. coli serotypes in raw milk and some varieties of raw milk cheese in Egypt. One Hundred and twenty samples of raw milk and white soft cheese (salted and unsalted) and hard cheese (Ras) collected from different areas in El Minofia governorate, Egypt were examined. The obtained results declared that coliform members including E. coli, Citrobacter diversus, C. freundii, Enterobacter aerogenes, E. agglomerans, E. cloacae, Klebsiella pneumoniae sub. spp. ozaenae and K. pneumoniae sub. spp. pneumoniae were isolated at varying percentage from examined samples. Kirby-Bauer disk diffusion test was used to examine bacterial susceptibility to ten antimicrobial agents including chloramphenicol, impenem, sulbactam+cefoperazone, ampicillin+sulbactam, amoxicillin+clavulanic acid, cefotaxime, gentamycin, amikacin, cefoperazone and tetracycline. Some strains showed multidrug resistant phenotype. The previously tested coliform strains, in order, showed resistance to AMC ; SAM & AMC ; AMC & CFP ; AMC & TE and C, AMC, CFP & TE antibiotics, while each showed sensitivity to the other tested antibiotics. The isolated E. coli proved to belong to 5 serotypes; O111:K58 (B9) EHEC, O 124 :K 72 (B 17 ) EIEC, O 119 :K 69 (B 19 ) EPEC, O 55 :k 59 (B 5 ) EPEC and untypable strains. In conclusion, this study provides meaningful data on the incidence of coliforms in raw milk cheese in Egypt and merely underlines the need for implementation of strict hygiene measures in preparation of raw milk cheese in Egypt. The most alarming trend detected in this study was the determination of antibiotic resistant and potentially toxigenic E. coli strains. This discovery makes ongoing surveillance of coliforms a high priority to identify emerging harmful strains within food production.