Type Of Carbon Source Research Articles

VARIASI SUMBER KARBON TERHADAP PRODUKSI BIOSURFAKTAN OLEH BAKTERI HALOFILIK ISOLAT TAMBAK GARAM KAJHU ACEH BESAR

This research has isolated one type of halophilic bacterial strain from the salt pond of Kajhu Village with the code KJ-AB2. This bacterium grew optimally at 10% (w/v) NaCl so it was classified as moderate halophilic bacteria. Several types of halophilic bacteria are known to produce biosurfactants. Biosurfactants are surface active agents that are widely used in several pharmaceutical, food dan petroleum industries. The production of biosurfactants is strongly influenced by the type of carbon source used for bacterial growth. Olive oil, palm oil, sunflower oil, glucose dan glycerol are types of carbon sources used to see their effect in producing biosurfactants. This isolate growed well on the five carbon sources with an average OD600 2.001±0.293 at 72 hours of fermentation. Biosurfactant production was measured by oil spreading test (OST) every 24 hours until 120 hours of fermentation. The highest OST value was obtained when KJ-AB2 isolate was grown on biosurfactant production media using olive oil, sunflower oil dan palm oil with an average OST value of 5.6±0.6 cm, 4.7±0.6 cm, dan 4.6±0.7 cm, respectively.

The importance of understanding the regulation of bacterial metabolism.

The importance of understanding the regulation of bacterial metabolism.

Microplastics a Novel Substratum for Polyhydroxyalkanoate (PHA)-Producing Bacteria in Aquatic Environments.

Polyhydroxyalkanoates (PHA) being biological polymers have attracted great attention. PHA have similar properties to that of synthetic plastic and are biodegradable. To discourage plastic pollution in the environment alternative solutions to the plastic pollution has to be readily available. High cost in production of PHA limits the production of these polymers at industrial scale. Bacteria are screened for PHA from diverse niches to meet the current requirements of cheap PHA production at industrial level. The microbial biofilm formed on the surface of microplastic could be a potential source in providing bacteria of economic importance. This paper is an attempt to search microplastic niche for potential PHA producers. PHA production variation was observed with different parameters such as type of carbon source, nitrogen source concentration and also time of incubation. Bacillus sp. CM27 showed maximum PHA yield up to 32.1% among other isolates at 48 h with 2% glucose as carbon source. Optimization of media leads to increase in PHA yield (37.69%) of CDW in Bacillus sp. CM27. Amino acid sequence of Bacillus sp.CM27 showed the presence of PhaC box with sequence, G-Y-C-M-G-G having cysteine in the middle of the box. The extracted polymer was confirmed by FTIR spectroscopy.Supplementary InformationThe online version contains supplementary material available at 10.1007/s00284-022-02929-y.

Bacterial Resuscitation from Starvation-Induced Dormancy Results in Phenotypic Diversity Coupled with Translational Activity Depending on Carbon Substrate Availability.

Dormancy is a survival strategy of stressed bacteria inhabiting a various environment. Frequent dormant-active transitions owing to environmental changes play an important role in functional redundancy. However, a proper understanding of the phenotypic changes in bacteria during these transitions remains to be clarified. In this study, orthogonal approaches, such as electron microscopy, flow cytometry, and Raman spectroscopy, which can evaluate phenotypic heterogeneity at the single-cell level, were used to observe morphological and molecular phenotypic changes in resuscitated cells, and RNA sequencing (RNASeq) was used to determine the genetic characteristics associated with phenotypes. Within 12h of the resuscitation process, morphological (cell size and shape) and physiological (growth and viability) characteristics as well as molecular phenotypes (cellular components) were found to be recovered to the extent that they were similar to those in active cells. The recovery rate and detailed phenotypic properties of the resuscitated cells differed significantly depending on the type or concentration of carbon sources. RNASeq analysis revealed that genes related to translation were significantly upregulated under all resuscitation conditions. The simpler the carbon source (e.g., glucose), the higher the expression of genes involved in cellular repair, and the more complex the carbon source (e.g., beef extract), the higher the expression of genes associated with increased energy production associated with cellular aerobic respiration. This study of phenotypic plasticity of resuscitated cells provides fundamental insight into understanding the adaptive fine-tuning of the microbiome in response to environmental changes and the functional redundancy resulting from phenotype heterogeneity.

Production and Characterization of Rhamnolipids Produced by Pseudomonas aeruginosa DBM 3774: Response Surface Methodology Approach.

Rhamnolipids are extensively studied biosurfactants due to their potential in many industrial applications, eco-friendly production and properties. However, their availability for broader application is severely limited by their production costs, therefore the optimization of efficacy of their cultivation gains significance as well as the information regarding the physio-chemical properties of rhamnolipids resulting from various cultivation strategies. In this work, the bioprocess design focused on optimization of the rhamnolipid yield of Pseudomonas aeruginosa DBM 3774 utilizing the response surface methodology (RSM). Six carbon sources were investigated for their effect on the rhamnolipid production. The RSM prediction improved the total rhamnolipid yield from 2.2 to 13.5 g/L and the rhamnolipid productivity from 11.6 to 45.3 mg/L/h. A significant effect of the carbon source type, concentration and the C/N ratio on the composition of the rhamnolipid congeners has been demonstrated for cultivation of P. aeruginosa DBM 3774 in batch cultivation. Especially, changes in presence of saturated fatty acid in the rhamnolipid congeners, ranging from 18.8% of unsaturated fatty acids (carbon source glycerol; 40 g/L) to 0% (sodium citrate 20 g/L) were observed. This demonstrates possibilities of model based systems as basis in cultivation of industrially important compounds like biosurfactants rhamnolipids and the importance of detailed study of interconnection between cultivation conditions and rhamnolipid mixture composition and properties.

Lignocellulosic hydrolysate based biorefinery for marine exopolysaccharide production and application of the produced biopolymer in environmental clean-up

The present study deals with the utilization of lignocellulosic hydrolysate-based carbon source for exopolysaccharide (EPS) production using newly reported marine Echinicola sediminis BBL-M-12. This bacterium produced 7.56 g L–1 and 5.32 g L–1 of EPS on supplementing 30 g L–1 glucose and 10 g L–1 xylose as the sole carbon source, respectively. Whereas on feeding Miscanthus hydrolysate (MCH) with glucose content adjusting to 20 g L–1, E. sediminis BBL-M-12 produced 6.18 g L–1 of EPS. The inhibitors study showed bacterium could tolerate higher concentrations of fermentation inhibitors include furfural (0.05%), 5-hydroxymethylfurfural (0.1%), vanillin (0.1%) and acetate (0.5%). Moreover, the EPS composition was greatly altered with the type and concentration of carbon source supplied, although β-D-Glucopyranose, β-D-Galactopyranose, and β-D-Xylopyranose were the dominant monomers detected. Interestingly, E. sediminis BBL-M-12 EPS revealed excellent environmental applications like clay flocculation, oil emulsification, and removal of humic acid, textile dye, and heavy metal from the aqueous phase.

Effect of carbon source conditions on response of nitrifying sludge to 3,5-dichlorophenol

Nutritional conditions of activated sludge had a significant influence on nitrification inhibition response. This study comprehensively investigated the inhibition of 3,5-dichlorophenol (3,5-DCP) on nitrification of activated sludge with different C/N ratios and carbon source types. The corresponding extracellular polymeric substances (EPS), microbial communities and functional genes were analysed. The results indicated that the addition of carbon source would reduce the nitrification activity and nitrification sensitivity to 3,5-DCP, and the order of the EC50 was sequenced as sodium acetate > methanol > glucose. The response mechanisms of activated sludge under diverse carbon source conditions to 3,5-DCP were summarised as follows. When the 3,5-DCP content was increased from 0.4 mg/L to 0.8 mg/L, the protein content increased from 73.2 ± 2.6 mg/g SS ∼122.4 ± 4 mg/g SS to 92.2 ± 11.2 mg/g SS ∼130.8 ± 9.6 mg/g SS in the tightly bound EPS (TB-EPS). The increase of protein content was attributed to cellular self-protection mechanisms. Furthermore, fluorescence characteristic analysis revealed that tyrosine and tryptophan in loosely bound EPS (LB-EPS) might account for higher EC50 in activated sludge fed with methanol and sodium acetate. In addition, the redundancy analyses (RDA) showed activated sludge with organics enriched the resistant species, such as Proteobacteria and Patescibacteria, while activated sludge without organics enriched the sensitive species, such as Ferruginibacter. Finally, the nitrification genes were found to be consistent with nitrification activity. Thus, the findings provide new insights into nitrification inhibition mechanism under different carbon source conditions.

Functional and phylogenetic diversity of sharks in the Northeastern Pacific

AbstractAimThe expansive spatial scale of pelagic communities and the difficulty in acquiring pelagic species' functional traits have stymied an understanding of marine community dynamics. We assembled and analysed a shark trait database and community phylogeny to identify the major axes of trait variation that define shark functional groups. We tested whether membership to biophysical macroecological strata is related to these functional or phylogenetic relationships.LocationNortheastern Pacific, 180–255°E and 0–50°N.TaxonSharks (Class: Chondrichthyes, superorder: Selachimorpha).MethodsWe built a community phylogeny and collected habitat, reproductive, somatic growth, trophic and dentition traits. We used principal coordinates analyses to identify axes of trait variation and hierarchical clustering to classify functional groups. We tested whether functional or phylogenetic relatedness determined species' membership to strata from five macroecological gradients: latitude, habitat type, thermal, carbon source and bathymetry.ResultsWe assembled 38 traits from 1225 records from 130 sources, 260 pictographs from seven sources and 631 teeth from 79 jaw specimens. Life history, r versus K selection, was responsible for the biggest division in the functional dendrogram. Vertical habitat preference, growth rates, diet and dental morphology generated further divisions between r‐ or K‐selected species. Vertical habitat preference, carbon source and biochemical habitat type were significantly dispersed or clustered on the functional dendrogram or phylogram.Main ConclusionsHabitat and reproductive traits were the most important trait suites driving shark functional diversity. Through ordination and clustering, we were able to associate major axes of trait variation to the membership of shark functional groups. The phylogram approximated well the functional dendrogram's backbone but was a poor substitute for the trait diversity at the tips. Given the long evolutionary history of sharks and coincident expansive trait diversity, merging functional and phylogenetic approaches was necessary to capture the dimensions of shark biodiversity.

The Effect of Carbon Sources and Whey Storage on Physicochemical and Organoleptic Properties of Nata de Whey

Whey is one of milk processing by-product that can be used for nata production. The goal of the study was to determine the carbon sources type and the optimal storage time of whey in the nata de whey production. A factorial completely randomized design with two factors was used as a research design. The first factor was type of carbon sources (glucose and sucrose) with two levels and the second factor was the storage time of whey with four levels (2, 3, 4, and 5 days). The main research showed that he addition of each 10 % sucrose and glucose did not significantly affect the water content, ash content, nata protein content, while showed a significant effect on nata fiber content, texture, and yield when it was stored for 2, 3, 4, and 5 days. The weighting results revealed that the optimal treatment was addition of 10% glucose for 3 storage days, moreover, the sucrose addition was optimum on the 2, 3, and 4 storage days. The organoleptic test showed that the color, texture, appearance, and acceptability were not significantly different, however the taste had significant effect. The best result of nata de whey production was 10% sucrose uses and the 3 days of storage time.

Poplar agroforestry systems in eastern China enhance the spatiotemporal stability of soil microbial community structure and metabolism

AbstractAgroforestry systems provide soil microorganisms with a rich variety of carbon sources and a relatively stable living environment. However, the effects of agroforestry systems on the metabolic dynamics of soil microorganisms have not been well studied, especially in the subsoil. To assess the vertical and seasonal variations in soil microbial metabolic capacity as a consequence of various planting systems, five typical planting systems, including three poplar agroforestry systems, were investigated. The Biolog EcoPlate method was used to determine the vertical and seasonal variations in soil microbial metabolic capacity. The average well colour development, carbon source utilization ability, and microbial diversity index values were higher throughout the soil profile, and highly stable with seasonal changes in the poplar (Populus × euramericana “Nanlin 895”) + wheat (Triticum aestivum L.) + soybean (Glycine max) and poplar + potherb mustard (Brassica juncea var. multiceps) agroforestry systems. Furthermore, the influence of the planting systems and seasonal changes on the metabolic activity of soil microorganisms decreased with an increase in soil depth. Over‐stocking chickens in the forest reduced the metabolic activity of soil microorganisms. It was also found that plants influenced soil microbial metabolism through affecting the available carbon source types. Therefore, agroforestry systems improved the metabolic potential of the soil microbial community. Our results demonstrated that soil microbial communities are affected by the planting system and soil depth. The findings enhance our understanding of the functional diversity of soil microorganisms in agroforestry systems.

Bacillus amyloliquefaciens WS-10 as a potential plant growth-promoter and biocontrol agent for bacterial wilt disease of flue-cured tobacco

BackgroundBacterial wilt disease caused by the soilborne bacterium Ralstonia solanacearum is a serious threat to flue-cured tobacco production. In this study, an indigenous disease suppressive Bacillus strain was isolated from the rhizosphere soil of healthy tobacco plants, and its biocontrol and plant growth promoting (PGP) potential were evaluated in in-vivo and in-vitro assays.ResultsThrough isolation and screening of 250 isolates, WS-10 was found to be the best candidate antagonistic strain against R. solanacearum (WS-001). In-vitro assays revealed that the isolated strain WS-10 (Bacillus amyloliquefaciens) showed an effective antagonistic activity against R. solanacearum WS-001 and several plant-pathogenic fungi. As promising PGP rhizobacteria, WS-10 had the ability of nitrogen fixation, solubilization of inorganic potassium and phosphate, and biosynthesis of indole-3-acetic. In a co-culture assay, it significantly inhibits the growth of WS-001. Our greenhouse experiments showed that the soil physicochemical properties and accumulation of dry matter contents in different plant parts (roots, stems, and leaves) were significantly increased in the presence of B. amyloliquefaciens WS-10. The soil treated with B. amyloliquefaciens WS-10 displayed significantly higher values of the average well color development index, the utilization ability of 6 types of carbon sources by rhizosphere microorganisms, and the diversity indices of the rhizosphere microbial communities. In planta assay, B. amyloliquefaciens WS-10 significantly reduced tobacco bacterial wilt disease incidence by up to 73.36, 43.82, and 86.82% under three different treatments by improving the functional diversity and biological activity of the soil microbial community.ConclusionsObtained findings suggested that B. amyloliquefaciens WS-10 had an excellent potential as a growth-promoting and biocontrol agent of tobacco bacterial wilt disease due to its multiple beneficial traits of nutrient solubilization and disease suppression. Thus, we conclude that B. amyloliquefaciens WS-10 was a high potential PGP and biocontrol strain for healthy production of tobacco crop.

Comparative Study of Polycaprolactone Electrospun Fibers and Casting Films Enriched with Carbon and Nitrogen Sources and Their Potential Use in Water Bioremediation.

Augmenting bacterial growth is of great interest to the biotechnological industry. Hence, the effect of poly (caprolactone) fibrous scaffolds to promote the growth of different bacterial strains of biological and industrial interest was evaluated. Furthermore, different types of carbon (glucose, fructose, lactose and galactose) and nitrogen sources (yeast extract, glycine, peptone and urea) were added to the scaffold to determinate their influence in bacterial growth. Bacterial growth was observed by scanning electron microscopy; thermal characteristics were also evaluated; bacterial cell growth was measured by ultraviolet-visible spectrophotometry at 600-nm. Fibers produced have an average diameter between 313 to 766 nm, with 44% superficial porosity of the scaffolds, a glass transition around ~64 °C and a critical temperature of ~338 °C. The fibrous scaffold increased the cell growth of Escherichia coli by 23% at 72 h, while Pseudomonas aeruginosa and Staphylococcus aureus increased by 36% and 95% respectively at 48 h, when compared to the normal growth of their respective bacterial cultures. However, no significant difference in bacterial growth between the scaffolds and the casted films could be observed. Cell growth depended on a combination of several factors: type of bacteria, carbon or nitrogen sources, casted films or 3D scaffolds. Microscopy showed traces of a biofilm formation around 3 h in culture of P. aeruginosa. Water bioremediation studies showed that P. aeruginosa on poly (caprolactone)/Glucose fibers was effective in removing 87% of chromium in 8 h.

An optimal carbon source can enhance the Paracin 1.7 titer and reduce carbon source consumption in cocultures of Lacticaseibacillus paracasei HD1.7 and Bacillus spp.

The low yield of bacteriocins limits their industrial use, and optimization of inoculation conditions and carbon sources (CS) are good strategies for improvement. In a previous study, we established three coculture systems of Lacticaseibacillus paracasei HD1.7 with Bacillus spp. Optimization of the inoculation conditions effectively increased the Paracin 1.7 titer. This study focused on the effects of the carbon source type (TCS) and carbon source concentration (CSC) on fermentation characteristics and bacteriocin titers. The results showed that the optimal TCS for bacteriocin production by different strain combinations is strain-specific. Adjusting the CS resulted in a higher Paracin 1.7 titer with less CS consumption. The B. subtilis group saved substrate consumption while yielding more Paracin 1.7 when 1T Glc was used as the CS. The optimal CS for the B. licheniformis group was 2T Fru. The B. pumilus group had the highest titer at 1T Fru. Gene expression analysis revealed that the appropriate TCS upregulated the relative expression levels of transmembrane protein genes (ptsG, fruA) and population sensing-related genes (prcK, prcR, and luxS), facilitating nutrient transport and information exchange. Structural equation modeling confirmed that controlling the CS can influence microbial growth and thus CS consumption, ultimately obtaining a satisfactory bacteriocin titer.

Applications of Sponge Iron and Effects of Organic Carbon Source on Sulfate-Reducing Ammonium Oxidation Process.

The typical characteristics of wastewater produced from seafood, chemical, textile, and paper industries are that it contains ammonia, sulfate, and a certain amount of chemical oxygen demand (COD). The sulfate-reducing ammonium oxidation process is a biochemical reaction that allows both ammonia and sulfate removal, but its low growth rate and harsh reaction conditions limit its practical application. Due to the adsorption properties of the iron sponge and its robust structure, it provides a suitable living environment for microorganisms. To reduce the negative impact on the environment, we employed 4.8 kg of sponge iron in a 2.0 dm3 anaerobic sequencing batch reactor (ASBR). We investigated the effects of the type and concentration of carbon sources on the performance of the sulfate-reducing ammonium oxidation (SRAO) process. The results demonstrated that during a start-up period of 90 days, the average ammonium removal efficiency and the sulfate conversion efficiency of the reactor containing the sponge iron were 4.42% and 8.37% higher than those of the reactor without the sponge iron. The addition of the sponge iron shortens the start-up time of this greenhouse gas-free denitrification process and reduces future costs in practical applications. The removal of total nitrogen (TN) significantly increased after adding organic carbon sources and then declined sharply, while the most considerable reduction of ammonium removal efficiency from 98.4% to 30.5% was observed with adding phenol. The performance of the group employing glucose as the carbon source was recovered on the 28th day, with the average ammonium removal efficiency increasing from 49.03% to 83.5%. The results of this simulation study will help the rapid start-up of SRAO in the water treatment industry and can precisely guide the application of the SRAO process for wastewater containing different organic carbon sources.

Penicillium brevicompactum as a novel source of natural pigments with potential for food applications

Synthetic colorants have gradually been replaced by natural pigments, mainly in the food industry. Among the several natural sources of pigments, filamentous fungi have gained special attention. However, the eventual biosynthesis of mycotoxins is a limitation for the practical use of this kind of microorganisms as pigment producers. In this work, Penicillium brevicompactum was studied for the first time as a potential pigment producer using submerged fermentation. The effect of several experimental parameters (culture medium composition, agitation speed, type of carbon source, temperature, concentration of supplements and carbon source, natural light and initial pH) in the production of pigments was evaluated. Under the optimal conditions (culture medium I containing lactose (20 g/L) and peptone/yeast extract (8/8 g/L), 23 °C, initial pH 7.0, 150 rpm, and natural light), a mixture of pigments (yellow, orange, and red) was obtained. This mixture is mycotoxin-free (ochratoxin A and mycophenolic acid) and presented promising antioxidant activity (FRAP: 58.58 ± 4.58 μmol Fe(II)/g; DPPH IC50: 18.48 ± 0.26 μmol TE/g and ABTS IC50: 28.38 ± 3.79 μmol TE/g). Furthermore, these pigments were proved to be very stable for a wide range of pH and temperatures. Overall, it was demonstrated that P. brevicompactum can be a safe source of natural pigments with interesting properties for the food industry.

Volatile Organic Compound Composition and Glandular Trichome Characteristics of In Vitro Propagated Clinopodium pulegium (Rochel) Bräuchler: Effect of Carbon Source.

Clinopodium pulegium (Rochel) Bräuchler (Lamiaceae) is an endangered species endemic to the Southern Carpathians. It is characterized by the production of high amounts of essential oils, which emit volatile organic compounds (VOCs) that have an essential role in biotic and abiotic stress responses and in plant–plant and plant–insect interactions. The present study was initiated to phytochemically examine the influence of different carbon sources in the nutrition medium on VOC emissions of micropropagated C. pulegium plants, using gas chromatography–mass spectrometry analysis of headspace VOCs. The volatile profiles were subjected to multivariate analysis with respect to the presence, concentration and type of carbon source in the nutrient medium. In addition, the effect of different carbohydrates on the density and size of the leaf glandular trichomes, the main structures involved in the emission of VOCs, was determined. A total of 19 VOCs, primarily belonging to mono- and sesquiterpenes previously described in plants, were tentatively identified. Six VOCs were produced at levels higher than 2% of the total VOC emission, dominated by pulegone, ß-pinene and menthone. Inclusion of the carbohydrates in the culture media affected the production of the main leaf trichome-associated volatile allelochemicals although the qualitative composition of the volatiles changed only slightly. Multivariate analysis showed that the concentration, rather than the carbohydrate type, influenced the VOC profile.

Physical and Chemical Fermentation Conditions Affect the Growth and Metabolite Production of Endophytic Fungi Athelia rolfsii

Background: The effort to explore antimicrobial agents isolated from an endophytic fungus of Coleus amboinicus resulted in the finding of a potential aromatic compound having methoxy, hydroxyl, and methyl groups produced by Athelia rolfsii. This compound exhibited antibacterial activities with IC50 values of <2 μg/mL against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Streptococcus mutans, and Pseudomonas aeruginosa. This study was aimed to determine the effect of varying fermentation conditions (types of media, carbon sources, nitrogen sources, temperature, and pH) on biomass, total metabolites, and bioactive compound production. Materials and Methods: The fungal cultures were subjected to various treatment conditions and incubated for 12 days at 25°C 160 rpm followed by analyzing the biomass, metabolite, and bioactive compound production. Results and Conclusion: The study found that although the maximum total metabolite production was achieved in the Tryptic Soy Broth medium, both biomass and bioactive compound production accumulated at the highest amount in Potato Dextrose Broth and Sabouraud Dextrose Broth media. Adding 1% of different types of carbon sources did not significantly enhance biomass, total metabolite, and bioactive compound production. Three types of organic nitrogen sources used in this study did not significantly affect biomass and total metabolite production, but adding peptone produced the highest amount of bioactive compound. Supplementing inorganic source of nitrogen to the culture medium decreased the production. While pH 5.5 was found to be the optimum condition for total metabolite production, pH 6–7 resulted in higher productivity of the bioactive compound. The total metabolite production was best produced at 25°C, whereas higher temperatures were needed to get optimum bioactive and biomass production. This study found that the total metabolite production was 7.8 times higher when the culture was grown in PDB medium supplemented with 1% sucrose and 1% peptone and incubated at 27°C at pH 6.5; whereas a 15% increase was observed in the bioactive compound production.

地膜覆盖对旱作春玉米农田土壤微生物碳源代谢的影响

PDF HTML阅读 XML下载 导出引用 引用提醒 地膜覆盖对旱作春玉米农田土壤微生物碳源代谢的影响 DOI: 10.5846/stxb202107302071 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金国际合作与交流项目（31961143017）；国家重点研发计划政府间国际科技创新合作重点专项（2021YFE0101300）；国家自然科学基金项目（31871575，41601328） Effects of film mulching on soil microbial carbon source metabolism in dry-farmland Author: Affiliation: Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:土壤微生物碳源代谢特征是评价土壤质量变化的重要指标。依托山西省东部晋中市寿阳县农业环境与作物高效用水科学观测试验站，采用Biolog-ECO微孔板技术探究覆膜（FM）与裸地不覆膜处理（LD）对旱作春玉米拔节期、灌浆期和收获期3个关键生育期不同土层深度下（0-10 cm、10-20 cm和20-30 cm）土壤微生物碳源代谢的影响。结果表明：1） FM措施可以显著增加土壤微生物对碳源的利用能力，显著提高了土壤微生物的优势度指数，但降低了土壤微生物的均匀度指数。2）在0-10 cm和10-20 cm土层，FM与LD处理土壤微生物碳源利用情况均表现为灌浆期较高，而20-30 cm土层，表现为收获期较高，差异均主要体现在碳水类、羧酸类、氨基酸类3大类碳源上。3）拔节期和灌浆期0-10 cm土层中FM处理的土壤微生物碳源利用能力显著高于LD处理；然而收获期0-10 cm土层中恰好相反，呈现出LD处理下土壤微生物碳源利用能力显著高于FM处理。4）此外，Pearson相关分析表明，FM处理土壤微生物对碳水类碳源和羧酸类碳源2大类碳源的利用能力主要与土壤有机碳（SOC）和全氮（TN）含量相关性较好，呈现出正相关关系；对氨基酸类碳源的利用能力主要与土壤含水量、pH、SOC、NH4+-N和NO3--N含量相关性较好。LD处理下，土壤微生物对碳水类、羧酸类和氨基酸类碳源的利用能力与土壤中TN含量的相关性较好，且呈现正相关关系。 Abstract:The metabolic characteristics of soil microbial carbon source are important indexes to evaluate the change of soil quality. Based on the scientific observation and experiment station of agricultural environment and efficient water use for crops in Shouyang County, Jinzhong City, eastern Shanxi Province, this study used Biolog-ECO microplate technology to explore the effects of film mulching (FM) and no film mulching (LD) on the carbon source metabolism of soil microorganisms in different soil depths (0-10 cm, 10-20 cm and 20-30 cm) in 3 key growth stages of dry-farming spring corn. The results show that:1) the FM measures can significantly increase the utilization ability of soil microorganisms to carbon sources, significantly improve the richness index of soil microorganisms, but reduce the evenness index of soil microorganisms. 2) In 0-10 cm and 10-20 cm soil layers, the utilization of microbial carbon sources in FM and LD treatments were higher in filling period, while in 20-30 cm soil layer, the harvest period was higher, and the differences were mainly reflected in 3 types of carbon sources:carbonaceous water, carboxylic acids and amino acids. 3) The utilization capacity of microbial carbon source in 0-10 cm soil layer of FM treatment was significantly higher than that of LD treatment at jointing stage and filling stage; However, in the 0-10 cm soil layer at harvest time, the oppositely is true, showing that the utilization capacity of soil microbial carbon source under LD treatment is significantly higher than that under FM treatment. 4) In addition, Pearson correlation analysis showed that under FM treatment in this study, the utilization ability of soil microorganisms to 2 kinds of carbon sources, namely, carbohydrate carbon source and carboxylic carbon source, had a good correlation with soil organic carbon(SOC) and total nitrogen(TN) content, showing a positive correlation; The utilization ability of amino acid carbon sources is mainly related to soil water content, pH, SOC, NH4+-N and NO3--N content. Under LD treatment, the utilization ability of carbon sources such as carbonaceous water, carboxylic acids and amino acids by soil microorganisms had a good correlation with TN content in soil, and showing a positive correlation. 参考文献 相似文献 引证文献

Suspension Culture and Somatic Embryogenesis of Korean Pine

Korean pine is an important afforestation tree species in Northeast China, which has a high ecological and economic value. Although regeneration of somatic embryogenesis using immature zygotic embryos of Korean pine as explants has been successful, it cannot be applied to automation and large-scale production. Therefore, we urgently need a method that can increase the output of somatic embryos (SEs) to meet the needs of large-scale production. We used Korean pine 1–1 and 1–100 cell lines as research materials to evaluate the effects of inoculum-density, culture time, orbiting speed, vessel volume, plant growth regulator (PGR) concentration, and carbon source on the proliferation of embryogenic tissue (ET). The somatic embryogenesis ability of ET cultured in different liquid suspension media was also evaluated. We found that during liquid suspension culture of Korean pine ET, the sedimented cell volume (SCV), fresh weight (FW) and dry weight (DW) were affected by inoculum-density, culture time, orbiting speed, 2,4-D concentration, 6-BA concentration and carbon source type. Fourty mg ⋅ mL−1 ET were transferred to a 200 mL Erlenmeyer flask containing 20 mL liquid medium, and cultured at 100 rpm/min for 14 days to obtain the maximum proliferation. In addition, we also found that SCV, FW and DW were higher when PGRs were reduced in the liquid suspension medium. The substitution of maltose for sucrose resulted in slow growth of cultures and limited SE yield (13 SEs g−1 FW). Although culture proliferation was high at 50 rpm, SE yield was inhibited by 48% compared with 100 rpm (50 rpm = 33 SEs g−1 FW; 100 rpm/min = 70 SEs g−1 FW). Cultivation in low-concentration PGR(1.15 μM ⋅ L−1 2,4-D, 0.25 μM ⋅ L−1 6-BA) and sucrose liquid medium at 100 rpm/min (80 SEs g−1 FW) could not only promote culture proliferation but also increase SE yield. The determination of the suspension culture scheme of Korean pine ET provides a reference for further expansion to bioreactor culture in the future and lays a foundation for the automation and scale of somatic embryogenesis of Korean pine.

Pine Wood and Sewage Sludge Torrefaction Process for Production Renewable Solid Biofuels and Biochar as Carbon Carrier for Fertilizers

This work presents the results of research on the thermo-chemical conversion of woody biomass–pine wood coming from lodzkie voivodship forests and sewage sludge from the Group Sewage Treatment Plant of the Łódź Urban Agglomeration. Laboratory scale analyses of the carbonization process were carried out, initially using the TGA technique (to assess activation energy (EA)), followed by a flow reactor operating at temperature levels of 280–525 °C. Both the parameters of carbonized solid biofuel and biochar as a carrier for fertilizer (proximate and ultimate analysis) and the quality of the torgas (VOC) were analyzed. Analysis of the pine wood and sewage sludge torrefaction process shows clearly that the optimum process temperature would be around 325–350 °C from a mass loss ratio and economical perspective. This paper shows clearly that woody biomass, such as pine wood and sewage sludge, is a very interesting material both for biofuel production and in further processing for biochar production, used not only as an energy carrier but also as a new type of carbon source in fertilizer mixtures.