Objective To evaluate the clinical efficacy of surgical operation combined with aminolevulinic acid-based photodynamic therapy (ALA-PDT) for the treatment of giant condyloma acuminatum. Methods Totally, 12 patients with giant condyloma acuminatum of the external genitalia and/or perianal region were enrolled, including 7 males and 5 females. Firstly, the warts were removed by surgical operation and bipolar electrocoagulation cautery. After wound healed, ALA-PDT was carried out at the lesional sites once every 7-10 days for 2-4 sessions. Results Within 6 months of follow-up, warts completely disappeared, and the acetowhitening test turned negative in all the 12 patients. Mild erythematous swelling occurred with pain and burning sensation after the operation, but no other adverse reactions such as infection, ulcer, or obvious scarring were observed. Conclusion Surgical operation combined with ALA-PDT is a relatively good choice for the treatment of condyloma acuminatum. Key words: Condylomata acuminata; Photochemotherapy; Aminolevulinic acid; Surgical procedures, operative; Clinical protocols