Scutellarin Research Articles

An retrospective analysis of adverse drug reaction in 103 cases of traditional Chinese medicine injection

Objective To understand the rules and characteristics of adverse drug reaction （ ADR） in Xinhua Hospital of Xinhua Medical Group （ Huainan City Anhui Province ）′s traditional Chinese medicine （ TCM） injection ca-ses,and to provide reference for rational and safety usage of TCM injection .Methods Reorganization was preformed on 663 cases of ADR report from January 2010 to November 2013 in this hospital .We selected 103 cases of TCM in-jection′s ADR reports,and made statistics and analysis according to patients′age,sex,drug source,report source,eval-uation,clinical features,system and organ ADR involved,etc.Results 103 cases of ADR reports involved in 26 kinds of drugs.All of them were vein medication .87.37%of the above cases were happened in 30 minutes with gender ratio of 60：43.54.36%were old patients over 60 with the highest incidence rate .33.98%were skin and appendages dis-orders.14.56%were Breviscapine ADR reports which was the most one .Conclusion It must insist on monitoring ADR of TCM injection and re-evaluation of market products ,to use TCM injection normative and rational ,and guaran-tee the medication security . Key words: TCM injection; ADR; ADR report; Rational medication

Effect of scutellarin on vascular impairment by hypoxia reoxygenation in isolated coronary artery from rat (LB562)

Scutellarin (SCU), a flavonoid from a traditional Chinese medicinal plant, is clinically used to treat cardiovascular diseases. In this study, we investigated the vasoprotective effects of SCU against hypoxia reoxygenation (HR) in isolated rat coronary artery (CA). HR treatment significantly impaired acetylcholine (ACh,0.001‐100 µM)‐induced endothelium‐dependent relaxation in isolated CA rings and these effects were reversed by pre‐incubation with SCU (500 µM). SCU pre‐incubation did not affect ACh response in normal CA without HR treatment. HR treatment did not significantly altered nitroglycerin (NTG, 0.001‐10 µM) induced endothelium‐independent vasodilation in CA and SCU (500 µM) had no significant effect on NTG response in CA with or without HR treatment. The PKG inhibitor Rp‐8‐Br‐cGMPS (4 µM) blocked both the vasorelaxative effects of SCU in CA rings without HR treatment and the vasoprotective effects of SCU in HR‐treated CA. These results demonstrate that SCU repairs HR‐induced vascular impairment in endothelium‐dependent vasodilation and might be via activation of the PKG pathway.Grant Funding Source: Supported by grants from the National Natural Science Foundation of China (Nos. 30960450 and 81173110) and Yunnan Provincial Science and Technology Department (Nos. 2011FA022, 2012BC012, 2008CD054, and 2008ZC111M).

Effect of scutellarin on JAK/STAT pathway in myocardial ischemia reperfusion rats (LB561)

Animal studies have demonstrated that scutellarin (SCU) limits damage after myocardial ischemia injury. However, the underlying molecular mechanisms of SCU protecting against myocardial injury induced by ischemia reperfusion (IR) are not well known. This study examined whether SCU protects against myocardial IR injury in rats is mediated by the Janus kinase and signal transducer and activator of transcription (JAK/STAT) pathway. Protein expression of JAK2/STAT3 and phosphorylative products were assessed by Western blot and immunohistochemistry methods in myocardial tissue from myocardial IR rats pretreated with SCU (45 and 90 mg/kg, iv). Western blot and immunohistochemistry assays showed that myocardial IR injury caused significant increases of phosphorylated JAK2 (p‐JAK2) and phosphorylated STAT3 (p‐STATS) levels in the rat myocardial IR model. SCU (45 and 90 mg/kg, iv) significantly reduced ischemic size, and decreased histological p‐JAK2 and p‐STATS expression. SCU attenuates myocardial IR injury which involved in JAK/STAT signaling pathway.Grant Funding Source: Supported by grants from the National Natural Science Foundation of China (Nos. 30960450 and 81173110) and Yunnan Provincial Science and Technology Department (Nos. 2011FA022, 2012BC012, 2008CD054, and 2008ZC111M).

Effects of breviscapine on epithelial-mesenchymal transition induced by transforming growth factor-β2 in human lens epithelial cells

Background Epithelial-mesenchymal transition (EMT) is a major event in the pathogenesis of posterior capsular opacification (PCO),and the expressions of α-smooth muscle actin (α-SMA) is the marker of EMT.Previous studies showed that breviscapine plays an important role in anti-fibrosis and suppression EMT,however,the mechanism of its effect on EMT in LECs is unclear.Objective Present study was to investigate the effect of breviscapine on the expression of α-SMA and fibronectin (FN) in human LECs induced by transforming growth factor-β2 (TGF-β2).Methods Human LECs strain,HLE-B3,was cultured and passaged in DMEM containing 10％ fetal bovine serum.Different concentrations of breviscapine (6.75,12.75,25.00,50.00 and 100.00 mg/L)were added into the medium for 24,48 and 72 hours respectively,and then cell cunting kit-8 (CCK-8) was used to evaluate the half maximal inhibitory concentration (IC50) of breviscapine to HLE-B3.In addition,HLE-B3 was subcultured at the density of 1 × 106/hole and divided into 4 groups.The cells were in free-serum medium as the normal control;the cells were exposed in 10 μg/L TGF-β2 as TGF-β2 group;while in the breviscapine group,10 mg/L of breviscapine was added into the culture medium and another group was the combination of 10 mg/L breviscapine and 10 μg/L of TGF-β2 treatment.Real-time PCR and Western blot were used to detect the mRNA expressions of α-SMA and FN as well as their protein in HLE-B3 72 hours after cultured.Results The inhibitory rate of breviscapine to HLE-B3 proliferation was gradually elevated with the increase of concentration of breviscapine,showing a significant inhibition of the cell proliferation among the different groups and at various time points (F =292.851,P=0.000;F =65.037,P=0.000).IC50 of HLE-B3 at 72 hours was 22 mg/L,and therefore,in rest of experiment 10 mg/L of breviscapine was used which was 1/2 only half of the IC50.α-SMA and FN were expressed in cultured normal HLE-B3.The expressing level of α-SMA mRNA and FN mRNA in the HLE-B3 was significantly different among the normal group,TGF-β22 group,breviscapine treatment group and combination group as well as at various time points (F =105.490,P =0.000 ; F =1041.414,P =0.000).Similarly,the protein expressions of α-SM A and FN in the HLE-B3 was significantly different among the four groups and different time points (F=136.872,P=0.000;F=119.820,P=0.000).The expression levels of α-SMA and FN mRNA and their proteins in HLE-B3 were remarkably increased in the 10 μg/L TGF-β2 group compared with the normal control group (at all P=0.000),and those in the combination group were obviously declined in comparison to the TGF-β2 group (P =0.001,0.001,0.001,0.010).No significant difference was found in the expressions of α-SMA and FN in the HLE-B3 between the breviscapine group and normal control group in both transcriptional level and protein level (P =0.551,0.292,0.551,0.360).Conclusions 10 mg/L breviscapine can arrest the proliferation and EMT of human LECs.This result suggests that using breviscapine may be a potential prophylactic approach in the prevention of PCO. Key words: Breviscapine; Lens epithelial cell; Epithelial-mesenchymal transition; Posterior capsular opacification

Inactivation of jack bean urease by scutellarin: Elucidation of inhibitory efficacy, kinetics and mechanism

In the present study, the inactivation effect of scutellarin (SL) on jack bean urease was investigated to elucidate the inhibitory potency, kinetics and mechanism of inhibition. It was revealed that SL acted as a concentration- and time-dependent inactivator of urease characteristic of slow-binding inhibition with an IC50 of 1.35±0.15mM. The rapid formation of the initial SL–urease complex with an inhibition constant of Ki=5.37×10−2mM was followed by a slow isomerization into the final complex with the overall inhibition constant of Ki*=3.49×10−3mM. High effectiveness of thiol protectors, such as L-cysteine (L-cys), 2-mercaptoethanol (2-ME) and dithiothreitol (DTT) significantly slowed down the rate of inactivation, indicating the strategic role of the active site sulfhydryl group in the blocking process. While the insignificant protection by boric acid and fluoride from the inactivation further confirmed that the active site cysteine should be obligatory for urease inhibition, which was also rationalized by the molecular docking study. The inhibition of SL on urease proved to be reversible since SL-blocked urease could be reactivated by DTT application and multidilution. The results obtained indicated that urease inactivation resulted from the reaction between SL and the sulfhydryl group.

A novel polyrotaxane-based delivery system for scutellarin: preparation, characterization, and in vitro evaluation

The safe and effective polyrotaxane-based drug delivery system could potentially increase the antiproliferative activity of antitumor medicine. A novel scutellarin–polyrotaxane (SCU–PR), in which scutellarin (SCU) was covalently bound to one of the hydroxyl groups of polyrotaxane (PR), was synthesized, and its characterization was further investigated by NMR, XRD, TG, DSC. The cytotoxicity of SCU–PR was assessed in vitro using human HCT116 and LOVO cell lines in results that the IC50 values of SCU–PR (1.03×10−6 and 1.01×10−6mol/L, respectively), which compared with those of free SCU (7.80×10−5 and 7.70×10−5mol/L, respectively), were lower. The valuable properties of SCU–PR will be potentially useful for its application on human colon cancer chemotherapies.

A New Procedure for Separation and Purification of Scutellarin from Erigeron multiradiatus

Abstract Objective To establish a new procedure for isolating scutellarin from Erigeron multiradiatus. Methods A proposed method was developed by combining macroporous resins with ODS column. Firstly, E. multiradiatus was extracted by ultrasound with 80% methanol. Preliminary separation was performed on macroporous resin column. The performance and adsorption characteristics of three macroporous resins, D140, D141, and D605, were compared and the enrichment procedure was optimized. Further purification was carried out by medium pressure liquid chromatography (MPLC) with ODS column. Results It was demonstrated that D141 had better extractive effects on scutellarin. The MPLC conditions were optimized as follows: 15% ethanol aqueous as mobile phase with flow rate at 2.5 mL/min. The yield and purity of the isolated scutellarin were 1.20 mg/g and 96.5%, respectively. Conclusion The overall procedure is efficient and low-cost, which is considered suitable for the separation and purification of scutellarin from E. multiradiatus. The results provide the scientific basis for developing and using scutellarin in clinic.

Simultaneous determination of three active constituents in Erigeron breviscapus by high- performance liquid chromatography

A rapid, simple and reliable high-performance liquid chromatographic (HPLC) method was developed for simultaneous determination of compound 1 (scutellarin), compound 2 [1,5-dicaffeoylquinic acid (1,5-DQA)] and compound 3 [1,3-dicaffeoylquinic acid (1,3-DQA)] inErigeron breviscapus. The three constituents were measured on a Zorbax XDB-C18column (4.6 mm × 150 mm, 5 μm) with a gradient elution of acetonitrile (0.5%) aqueous phosphoric acid at wavelength of 335 nm and a flow rate of 1.0 ml/min. Linearity of each standard was established in the concentration range of 19.375 to 310.0 μg/ml for compound 1, 6.125 to 98.0 μg/ml for compound 2, and 3.875 to 62.0 μg/ml for compound 3, respectively, with correlation coefficient r > 0.9990. Average recoveries (n = 6) of compounds 1 to 3 were 100.3% with a relative standard deviations (RSD) of 1.5%, 98.9% with a RSD of 1.9%, 98.6% with a RSD of 2.0%, respectively. The proposed method was successfully applied to simultaneously determine the three active constituents in E. breviscapus from different habitats and germplasm resources for the first time. Key words: high-performance liquid chromatography (HPLC), Erigeron breviscapus, scutellarin, 1,5-dicaffeoylquinic acid, 1,3-dicaffeoylquinic acid.

Protective effect of polysaccharide from the root of Rhizoma Corydalis on focal ischemic cerebral infarct induced by middle cerebral artery occlusion in rats

To investigate the effect of polysaccharide from the root of Rhizoma Corydalis (YhPS-1) on focal cerebral infarct in ischemia-reperfusion injured rats, sixty Wister rats were randomly divided into six groups (n = 10): sham group, control group, YhPS-1-treatment groups (20, 40, and 80 mg/kg), and positive medicine breviscapine table group, respectively. The models of focal cerebral infarct and carotid thrombosis were established, and neurobehavioral scores, the area of cerebral infarct, water content of brain, clotting time and thrombosis time were used to evaluate the effect of YhPS-1. Experiments indicated that YhPS-1-treated groups significantly improved neurological deficits, reduced the area of cerebral infarct and brain water content, and prolonged clotting time and thrombosis time in comparison with control group. These findings suggested that YhPS-1 played a crucial protective role in cerebral ischemia-reperfusion injury. Key words: Polysaccharide of Rhizoma Corydalis, cerebral infarct, blood clotting time, thrombosis time, free radicals.

Protective effect of breviscapine on human hepatocytes under hypoxia/re-oxygenation and its possible mechanism

To explore the protective effect of breviscapine on human hepatocytes (L-02) under hypoxia/re-oxygenation (H/R) and elucidate its possible mechanism. A in vitro model of H/R was employed to mimic H/R injury of graft organ. L-02 cells were randomly divided into 3 groups: control, H/R and breviscapine treatment (pre-treated with breviscapine and H/R). After a 10 h hypoxic culturing under 1% O(2), 94% N(2) and 5% CO(2), L-02 cells received oxygen for 1, 2, 4, 8, 16 and 24 h respectively. MICA mRNA and protein levels were detected by quantitative real-time polymerase chain reaction (PCR) and Western blot. And the activity of natural killer (NK) cell cytotoxicity for L-02 was measured by lactate dehydrogenase (LDH) release assay. After blocking I/R treatment with NKG2D antibody, the activity of NK cell cytotoxicity for L-02 was detected. After 10 h hypoxia, MICA mRNA and protein levels significantly increased from 1 h, stayed up-regulated until 8 h and then went back to normal level after reoxygenation versus the control group. The activity of NK cell cytotoxicity for L-02 under the treatment of H/R increased markedly from 1 h post-reoxygenation and stayed up-regulated from 1 h to 8 h versus the control group. After hypoxia, L-02 cells were blocked with NKG2D antibody and the NK cell cytotoxicity for L-02 significantly decreased versus the I/R group. The administration of breviscapine significantly lowered the mRNA and protein levels of MICA in L-02 under I/R and then significantly decreased the NK cell cytotoxicity. The process of I/R may mediate the NK cell cytotoxicity activity toward to L-02 by inducing a strong increase of MICA at mRNA and protein levels in L-02 cells. And the administration of Breviscapine significantly reduces the NK cell cytotoxicity for L-02 under I/R through the down-regulated expression of MICA.

Effect of breviscapine on lung injury in children undergoing open heart surgery with cardiopulmonary bypass

Objective To investigate the effect of breviscapine on lung injury in children undergoing open heart surgery with cardiopulmonary bypass(CPB).Methods Forty-five ASA Ⅱ or Ⅲ children aged 3-65 months weighing 5-21 kg undergoing open heart surgery with CPB were randomly assigned to 3 groups ( n =15 each):control group (group C),low dose breviscapine group (group B1 ) and high dose breviscapine group (group B2).Normal saline 15 ml(group C),breviscapine 0.5 mg/kg (group B1 )or 1.0 mg/kg(group B2 ) were injected iv over 30min after anesthesia induction.Blood samples were taken before operation ( T0 ),at 30 min and 1 h of aortic unclamping (T1,T2 ),at 3 h and 6 h after operation (T3,T4 ) for determination of plasma procalcitonin (PCT)and neutrophil elastase(NE) concentrations.PaO2 and PaCO2 were recorded at T0,T3,T4 for caculation of oxygenation index (OI) and alveolo-arterial oxygen partial pressure difference (PA-a O2 ).Results There were no significant differences in OI and PA-a O2 among the 3 groups( P ＞ 0.05).Plasma concentration of PCT was higher at T1～4in 3 groups,and plasma concentration of NE was higher at T1 in group C than that at T0 ( P ＜ 0.01 ).Plasma concentrations of PCT and NE were lower in groups B1 and B2 than in group C ( P ＜ 0.01).There were no significant differences in plasma concentrations of PCT and NE between groups B1 and B2 ( P ＞ 0.05).Conclusion Breviscapine(0.5,1 mg/kg) can inhibite systemic inflammatory response and attenuate lung injury in children undergoing open heart surgery with CPB. Key words: Flavonoids; Cardiopulmonary bypass; Cardiac surgical procedures; Respiratory distress syndrome, adult; Child

Scutellarin-dependent inhibition of collagen biosynthesis in cultured fibroblasts

The effects of the flavonoid compound scutellarin (SCUT) on collagen biosynthesis, prolidase activity, expression of β1 integrin, insulin-like growth factor-I (IGF-I) receptor and the transcription factor NF-κB were evaluated in human dermal fibroblasts. Confluent fibroblasts were treated with micromolar concentrations (10–30 µM) of SCUT for 24 h. It was found that a SCUT-dependent decrease in collagen biosynthesis was accompanied by an increase in prolidase activity. Since the IGF-I receptor (IGF-IR) is the most potent regulator of both collagen biosynthesis and prolidase activity, and prolidase is regulated by β1 integrin signalling, the effect of SCUT on IGF-IR and β1 integrin receptor expressions were evaluated. It was found that the exposure of the cells to SCUT contributed to an increase in IGF-IR and β1 integrin receptor expressions. This was accompanied by an increase in expression of NF-κB, the known inhibitor of collagen gene expression. These data suggest that the SCUT-dependent decrease of collagen biosynthesis in cultured human skin fibroblasts results from activation of NF-κB, which is responsible for the down-regulation of collagen gene expression.

Study on the efficacy of breviscapine in the treatment of vascular cognitive impairment

Objective To investigate the efficacy of breviscapine in the treatment of vascular cognitive impairment.Methods A self-controlled trial was carried out in 36 patients with vascular cognitive impairment.36 cases were treated with breviscapine injection 50mg intravenous infusion daily for 3 weeks.At the same time,all cases were also given citicoline 0.75g/d and enteric-coated aspirin 100mg/d as routine treatment.The means of the evaluation on therapeutic effect included MMSE and ADL,and the adverse reactions were also observed.Results After the treatment,the MMSE scales increased from(18.75 ± 3.25)to(21.62 ± 3.58)(t=2.52,P＜0.05),Meanwhile,ADL scales declined from(45.65 ±3.36)to(42.33 ±4.18)(t=3.71,P＜0.05).There was significant difference on the MMSE and ADL between the pre and post therapy.No obvious side effects were found.Conclusion The results indicated that the breviscapine injection is an effective medicine in the treatment of vascular cognitive impairment,while the adverse reactions were few. Key words: Dementia,vscular; Breviscapine

Clinical effect of Breviscapine on the vision restoration of postoperative advanced glaucoma

Objective To observe the effect of Brevisapine on the vision restoration of advanced glaucoma after the intraocular pressure （IOP） was controlled by surgery. Methods 96 cases （128 eyes） of advanced glaucoma had undergone surgery for controlling of IOP. Then they were treated by Brevisapine for averagely 3 months. The vision was observed in the 12 -72 months period of follow-up. Results All of the patients had got an improved vision of 0. 1 - 0.3. There was no side-effect or rebound phenomenon. Conclusion Brevisapine can improve the vision of advanced glaucoma after the lOP had been controlled. It is a safety and effective traditional Chinese herb. Key words: Brevisapine ; advanced glaucoma; vision; intraocular pressure

Clinical observation of therapeutic effects with irbesartan and breviscapine on diabetic nephropa thy

Objective To observe the effect of irbesartan and breviscapine on diabetic nephropathy.Methords 70 patients with diabetic nephropathy were divided randomly into two groups.The experimental group (n=36) were treated with irbesartan and breviscapine,and the control group(n=34)were treated with compound Dartshen injection.The contents of urinary protein,NAG,renal and serum potassium were tested,and difference between experimental group and control group were compared.Results The uses of irbesartan combined with breviscapine reduced urinary albumin excretion rate significantly(P<0.01)and improved the blood hypercoagulable state(P<O.05).Conclusions Combination of irbesartan and breviscapine is effective in the treatment of diabetic nephropathy. Key words: diabetic nephropathy; irbesartan; breviscapine

Binding behaviors of scutellarin with α-, β-, γ-cyclodextrins and their derivatives

A series of cyclodextrin/scutellarin inclusion complexes were prepared from α-cyclodextrin, β-cyclodextrin and 2-hydroxypropyl-β-cyclodextrin with scutellarin (SCU), and their inclusion complexation behaviors, such as stoichiometry, complex stability constants and inclusion mode, were investigated by means of UV/Vis spectroscopy, 1H NMR and 2D NMR. The results showed that the SCU could be efficiently encapsulated in the cyclodextrin cavity in aqueous solution to produce complexes that were more soluble than free SCU. The enhanced binding ability of cyclodextrins towards SCU was discussed from the viewpoint of the size/shape-fit and multiple recognition mechanism between host and guest.

Nitric Oxide and Catalase-sensitive Relaxation by Scutellarin in the Mouse Thoracic Aorta

The vascular activity of scutellarin (SCU), a flavonoid isolated from a Chinese traditional medicinal plant, was investigated in isolated thoracic aortic rings of mice. SCU-induced dose-dependent relaxation of phenylephrine (1 microM) stimulated contractions. This relaxation was reduced by endothelium removal, significantly reduced by both the nitric oxide synthase inhibitor (Nomega-nitro-L-arginine methylester, 300 microM) and slightly limited by the soluble guanylyl cyclase inhibitor (1 H-[1,2,4] oxidazolol [4,3-a] quinoxalin-1-one, 100 microM). The catalase inhibitor (3-amino-1,2,4-triazole, 50 mM) augmented the constriction and blocked the lowest SCU concentration relaxation, whereas catalase addition was without effect. Preincubation with 300 and 1000 microM SCU significantly suppressed the contractile dose-response to phenylephrine, causing both a significant rise in half maximal effective concentration and a decrease in the maximal developed force. Western blot analysis showed that SCU inhibition of contraction was independent of reductions in myosin light chain phosphorylation. These results suggested that SCU relaxation was predominantly endothelium dependent and likely involved the catalase-sensitive nitric oxide synthase signaling pathway, without loss of myosin phosphorylation. The potential clinical use of SCU may prove to be effective in increasing vasoreactivity, independently of smooth muscle contractile activity that is mediated by the 20-kDa myosin light chain phosphorylation.

Simultaneous Determination of Three Flavonoids in Rat Plasma by RP-LC After Oral Administration of the Total Flavonoids of Scutellaria barbata

A liquid chromatographic method for the simultaneous determination of three flavonoids, scutellarin (SCU), isoscutellarein-8-O-glucuronide (ISO) and luteolin (LUT) in rat plasma was developed and validated. Following a single-step liquid–liquid extraction with ethyl acetate, the analytes and internal standard (IS) (rutin) were successfully separated on a Diamonsil C18 column using a mobile phase composed of acetonitrile (A)–0.2% phosphoric acid aqueous solution (B) (0–5 min, 20% A–29% A; 5–25 min, 29% A, v/v) at a flow rate of 1.0 mL min−1. The linear range was 0.044–2.20 μg mL−1 for SCU, 0.042–2.08 μg mL−1 for ISO, and 0.056–2.80 μg mL−1 for LUT, with the correlation coefficients of 0.9995, 0.9989 and 0.9963, respectively. The limit of quantification of SCU, ISO and LUT were 44, 41.6 and 56 ng mL−1, respectively. The accuracy of assay was between 88.4 and 103.0%. The inter-day and intra-day precisions (RSD) were less than 10.5%. The developed method was simple, rapid and applied successfully to study the pharmacokinetics of SCU, ISO and LUT after oral administration of the total flavonoids of Scutellaria barbata.

Simultaneous determination of Total chlorogenic acid and scutellarin in guantong injection by HPLC

目的 建立冠通注射液含量测定方法 .方法 采用HPLC法,色谱柱为Diamonsil C18(250 mm×4.6mm,5 μm);流动相为乙腈-0.4%磷酸溶液,线性梯度洗脱.采用二极管阵列检测器,选取与绿原酸相同紫外吸收图谱的峰为总绿原酸峰,在327 nm检测波长处测定总绿原酸、灯盏花乙素含量.结果 绿原酸在进样量0.333 μg～8.880 μg范围内呈良好的线性关系,回收率99.67%(RSD=1.39%);灯盏花乙素在进样量0.0804 μg～1.34 μg范围内呈良好的线性关系,回收率100.89%(RSD=0.72%).结论 方法 准确可行,可用于该制剂的质量控制。

Influence of Breviscapine Injection on IGF of Pregnant Rats and Growth of Fetus Rats

Objective To discuss the influence of Breviscapine Injection on concentration of serum IGF-Ⅱ in pregnant rats and changes of fetus rat body weight, body length, and body-mass index. Methods Radio-immunity was used to determine the concentration of serum IGF- Ⅱ in mother rats and fetus rats. Body weight and body length were surveyed,the IGF- Ⅱ concentration of fetus rats in the FGR group was lower than the control group (P＜0.05) and IGF- Ⅱ concentration control group＞the FGR+intervention group＞ the FGR group, and the difference between each group was significant (P＜0.05).Breviscapine can treat fetal growth retardation effectively. Key words: Breviscapine Injection; IGF- Ⅱ; Fetal growth retardation