Blue Fluorescence Research Articles

Nitrogen-doped MoS2 QDs as fluorescent probes for sensitive detection of curcumin and cell imaging

BackgroundCurcumin has been used in traditional medicine because of its pharmacological activity, including antioxidant, antibacterial, anticancer, and anticarcinogenic properties. Therefore, sensitive and selective monitoring of curcumin is highly demand for practical application. ResultsIn this study, we describe the construction of a fluorescence method for curcumin assay based on nitrogen-doped MoS2 quantum dots (N–MoS2 QDs). The N–MoS2 QDs are constructed by a solvothermal method using sodium molybdate and Cys as precursors. With the addition of curcumin, the bright blue fluorescence of N–MoS2 QDs is quenched by the inner filter effect (IFE). The QDs emitted bright blue fluorescence and could be quenched by the addition of curcumin via IFE. The dynamic range is the range of 0.1–10 μM for curcumin detection, with a detection limit of 59 nM. N–MoS2 QDs were applied for curcumin assay in real samples with good recovery. In addition, the N–MoS2 QDs exhibited relative low cytotoxicity and could be applied for fluorescence-based imaging in biological samples. SignificanceOur study indicates that the sensor possesses good selectivity to monitor curcumin in water samples, human urine samples, ginger powder samples, mustard samples, and curry samples with satisfactory recoveries. The N–MoS2 QDs possess less cytotoxicity with excellent biocompatibility and were applied for in vitro cell imaging.

Dual Fluorescence and Phosphorescence Emissions from Dye-Modified (NCN)-Bismuth Pincer Thiolate Complexes.

We report the synthesis, characterization, and photophysical properties of four new dye-modified (NCN)Bi pincer complexes with two mercaptocoumarin or mercaptopyrene ligands. Their photophysical properties were probed by UV/vis spectroscopy, photoluminescence (PL) studies, and time-dependent density functional theory (TD-DFT) calculations. Absorption spectra of the complexes are dominated by mixed pyrene or coumarin π → π*/n(pS) → pyrene or coumarin π* transitions. While unstable toward reductive elimination of the corresponding disulfide under irradiation at room temperature, the complexes provide stable emissions at 77 K. Under these conditions, coumarin complexes 2 and 4 exhibit exclusively green phosphorescence at 508 nm. In contrast, the emissive properties of pyrene complexes 1 and 3 depend on the excitation wavelength and on sample concentration. Irradiation into the lowest-energy absorption band exclusively triggers red phosphorescence from the pyrenyl residues at 640 nm. At concentrations c < 1 μM, excitation into higher excited electronic states results in blue pyrene fluorescence. With increasing c (1-100 μM), the emission profile changes to dual fluorescence and phosphorescence emission, with a steady increase of the phosphorescence intensity, until at c ≥ 1 mM only red phosphorescence ensues. Progressive red-shifts and broadening of steady-state excitation spectra with increasing sample concentration suggest the presence of static excimers, as we observe it for concentrated solutions of pyrene. Crystalline and powdered samples of 1 indeed show intermolecular association through π-stacking. TD-DFT calculations on model dimers and a tetramer of 1 support the idea of aggregation-induced intersystem crossing (AI-ISC) as the underlying reason for this behavior.

Ratiometric fluorescent probe with AIE characteristics for hypochlorite detection and biological imaging

It is very important and highly valuable to detect ClO− in samples and living cells with accuracy and speed. In this work, a novel fluorescent probe NA was prepared from 4-bromo-1,8-naphthalic anhydride by acylation reaction and Suzuki coupling reaction and used for the detection of ClO−. Thiomethyl serves as the recognition group for probe NA, while naphthalimide serves as fluorescent chromophore. The probe exhibited an extremely pronounced blue shift from yellow to blue fluorescence within 1 min after the addition of hypochlorite (ClO−). The probe demonstrates high sensitivity to ClO− with a limit of detection (LOD) of 1.22 µM. Also, probe NA demonstrates excellent selectivity and immunity to interference. Additionally, simple fluorescent test strips containing probe NA were prepared in this study, enabling rapid detection of ClO− in water samples. And NA had been effectively used to image endogenous and exogenous ClO−fluorescence in living cells. The results suggest that probe NA has significant potential for portable detection and biological applications.

Cellulose-based colorimetric/ratiometric fluorescence sensor for visual detecting amines and anti-counterfeiting

Many amines with high toxicity always cause a serious threat to the ecological environment and human health; thus, their detection is important. Herein, a dual-mode colorimetric and ratiometric fluorescent sensor based on cellulose for detecting amines has been constructed by a new strategy. This sensor is made of a “negative response” indicator (Lum-MDI-CA) and a “positive response” indicator (perylene tetracarboxylic acid, PTCA). Lum-MDI-CA was obtained by attaching luminol onto cellulose chains, which emitted blue fluorescence and was quenched upon contact with amines. A possible mechanism of fluorescence quenching phenomenon is proposed by the intramolecular charge transfer (ICT) of Lum-MDI-CA. Subsequently, by simply mixing Lum-MDI-CA with PTCA, a dual-mode fluorescence sensor was designed for visual detection and classification of amines. When adding ammonia (NH3), morpholine (MOR), benzylamine (BNZ), diethylamine (DEA), and triethylamine (TEA), respectively, the dual-mode sensor showed visible different color changes under both UV light and daylight. In addition, owing to the excellent processibility and formability of cellulose acetate backbone, the prepared sensor can be easily processed into different material forms, including inks, coatings, films, and fibers, which still exhibit excellent fluorescence emission. Such sensors based on cellulose fluorescent materials are of great value in anti-counterfeiting and information encryption.

Bio-inspired multimimimetic synergistic strategies Cs3Bi2Br9/ZnS organosilicon antifouling flexible films: Coupled micro/nanostructured surfaces, fluorescence, and reactive oxygen species mechanisms

Constructing multifaceted bio-inspired environmentally friendly films combining the synergistic advantages of multiple bionics can improve the static antifouling effect of antifouling-releasing silicones and mitigate the toxicity to marine ecology. In this work, based on the multiple anti-fouling mechanisms of fluorescent corals, designed Cs3Bi2Br9/ZnS/PDMS (CZP) flexible fluorescent films with surface microstructures and interpenetrating network Moreover, the blue fluorescence in the CZP films can be maintained for a long time in seawater environment (attributed to bismuth-based chalcogenides), and the formation of the heterostructure of Cs3Bi2Br9/ZnS (CBB/ZS) improves the ability of releasing reactive oxygen species (hydroxyl radical, superoxide radical, and singlet-linear oxygen).The CZP films obtained excellent antifouling effect under static conditions with antimicrobial rate up to 90.51 % and anti-algae adhesion to diatoms up to 96.60 %, which was attributed to the synergistic effect of the fluorescence functionality, micronized structure, and free radical release. Consequently, this work provides a promising avenue for the development of marine antifouling silicon-based films for efficient and environmentally friendly applications.

Preparation and Application of a Sulfur-Doped Fluorescent Carbon Dots with Aggregation-Induced Emission Character.

As a new type of zero-dimensional nanomaterial, carbon dots are widely applied in various fields. However, most of the carbon dots have aggregation fluorescence quenching properties, which limited their practical applications. In this study, a novel sulfur-doped carbon dots (S-CDs) was prepared by solvothermal method. The properties of the S-CDs in ethanol solution and in solid state were investigated respectively. The results showed that the S-CDs have an excited wavelength dependent emission of blue fluorescence in ethanol solution, and have orange fluorescence emission in solid state and composite films, indicating the prepared S-CDs has aggregation-induced emission (AIE) performance. The main reason was that the presence of S-S bonds and the intramolecular rotation of aromatic rings were limited in solid state, resulting in its emission of orange fluorescence. Furthermore, the S-CDs could be applied to identify fingerprints, anti-counterfeiting.

Microwave-assisted Facile Synthesized Carbon Dots as "on-off-on" Fluorescence Probes for Mercury and Iodine Ions in Bio-samples and Cell Imaging.

A kind of nitrogen and sulfur co-doped CDs (N, S-CDs) was facilely synthesized using thiourea and citric acid as precursors, which established an "on-off-on" fluorescence probe to sequential detecting mercury and iodine ions inside water and biology samples. Under 360nm excitation, CDs emit blue fluorescence with an optimal emission peak of 425nm (on). The fluorescence of CDs experiences a significant quenching effect upon interaction with Hg2+ ions due to the electron transfer between CDs and Hg2+. This quenching effect is subsequently recovered upon the addition of I- owing to the formation of complexes between Hg2+ and I-. The probe exhibits high selectivity and sensitivity toward Hg2+ and I- with broad linearity in the range of 5-50μM and 15-60μM, respectively, and a low detection limit of 14.336nM and 38.213nM, respectively. The constructed fluorescence probe N, S-CDs has been successfully applied to the detection of Hg2+ and I- in water and biological samples with great recoveries. More importantly, the bioimaging study demonstrated that N, S-CDs are suitable for live monitoring in biological imaging scenarios of Hg2+ and I- in living cells.

A coumarin-naphthalimide-based ratiometric fluorescent probe for nitroxyl (HNO) based on an ICT-FRET mechanism

Nitroxyl (HNO) is an important reactive nitrogen that is associated with various states in physiology and pathology and plays a unique function in living systems. So, it is important to exploit fluorescent probes with high sensitivity and selectivity for sensing HNO. In this paper, a novel ratiometric fluorescent probe for HNO was developed utilizing intramolecular charge transfer (ICT) and fluorescence resonance energy transfer (FRET) mechanisms. The probe selected coumarin as energy donor, naphthalimide as energy receptor and 2-(diphenylphosphino)benzoate as the sensing site for detecting HNO. When HNO was not present, the 2-(diphenylphosphino)benzoate unit of the probe restricted electron transfer and the ICT process could not occur, leading to the inhibition of FRET process as well. Thus, in the absence of HNO the probe displayed the intrinsic blue fluorescence of coumarin. When HNO was added, the HNO reacted with the 2-(diphenylphosphino)benzoate unit of the probe to yield a hydroxyl group which resulting in the opening of ICT process and the occurring of FRET process. Thus, after providing HNO the probe displayed yellow fluorescence. In addition, the probe showed good linearity in the ratio of fluorescence intensity at 545 nm and 472 nm (I545 nm/I472 nm) with a concentration of HNO (0.1–20 μM). The probe processed a detection limit of 0.014 μM and a response time of 4 min. The probe also specifically identified HNO over a wide pH scope (pH = 4.00–10.00), including physiological conditions. Cellular experiments had shown that this fluorescent probe was virtually non-cytotoxic and could be applied for ratiometric sensing of HNO in A549 cells.

Evaluation Of Some Aspergillus Mycotoxins In Patients With Bronchitis In Al-Najaf City

This research aimed to establish whether the Aspergillus species identified from bronchitis patients were mycotoxigenic or non-mycotoxigenic fungi in sputum and serum samples. A total sample of 170 subjects with ages ranging from 15 to 72 years— were obtained from Al-Najaf Province (Al-Sadr Medical City) between December 2023 and April 2024, divided into patient (n=176) and healthy groups (n=34). The Aspergillus genera isolated were cultured on coconut agar medium and screened under UV light (360 nm); each sputum and serum sample was also tested using the ELISA technique. All Aspergillus strains exhibited blue fluorescence on Coconut Cream Agar (CCA) media under UV light, confirming positivity. The study identified production of aflatoxins B1 and ochratoxins-A in sputum and serum samples among both patients with bronchitis and control groups. The results showed a significant difference in patient groups by samples (P≤ 0.05) while the healthy groups showed a non-significant (P&gt;0.05) difference. Most Aspergillus spp. Isolated from bronchitis patients were found to produce aflatoxin B1 and ochratoxin-A on coconut agar medium— mycotoxins extracts included sputum (about 68.4% for aflatoxins-B1 and 41.7% for serum samples) and sputum (about 77.6% for ochratoxin-A and 91.7% for serum samples) taken from bronchitis patients, which were identified using ELISA technique.

AIE active blue light emitting pyridine functionalized quinoxaline and pyridopyrazine derivatives exhibiting reversible acidofluorochromism and thermal sensitivity

This work presents a systematic study of five novel closely related pyridine-substituted quinoxaline and pyridopyrazine derivatives 3a-e. Modifying the quinoxaline framework by introducing electron donating and accepting units led to fine-tuning of optical, thermal, and electrochemical properties. The single crystal X-ray diffraction studies revealed a Y-shaped structure with a herringbone packing arrangement. These compounds showed aggregation-induced emission (AIE) with the formation of J-aggregates. These compounds emitted blue fluorescence with one of the compounds showing CIEy as low as 0.08 in CHCl3 and 0.09 in the solid state. The band gaps were evaluated using UV-visible spectroscopy and density functional theory (DFT). The optical band gaps in the solid state were in the 2.81–3.05 eV range. They exhibited reversible switching of emission properties upon treatment with trifluoroacetic acid (TFA) followed by triethylamine (TEA) in both solution and solid states, which was utilized to fabricate filter paper strips to detect volatile acids. The emission of compounds also displayed linear temperature dependence.

Preparation of ZnO@SiO2 composite with controllable sizes and tunable fluorescence via alkali-free strategy

In this paper, we present a simple way to achieve the controllable synthesis of composite ZnO@SiO2 materials. γ-aminopropyltriethoxysilane (APTES) was chosen as the silicon source, and pre-synthesized zinc bromoacetate (ZnBA) was selected the functional metal precursor. By simply regulating the water–ethanol volume ratio, it was possible to simultaneously regulate the sizes of both the composite material and the metal nanoparticles. With an increase in the hydroalcoholic volume ratio from 1:1–3:1, the sizes of the silica materials were increased from 615 to 3020 nm, while the sizes of the ZnO nanoparticles were increased from 3.75 to 6.25 nm. Three ZnO@SiO2 materials with blue, green, and yellow fluorescence were obtained. More importantly, the hydroalcoholic volume ratio and the particle size of the material showed a good linear relationship. In addition, a “rapid nucleation–aggregation growth” mechanism was proposed, and the controlled growth of metal oxide nanoparticles was explored.

A dual-functional NIR fluorescence probe for detecting hypochlorous acid and bisulfite in biosystem

BackgroundBisulfite (HSO3−) serves as a bleaching agent, antioxidant, antimicrobial, and regulator of enzymatic reactions in biosystem. However, abnormal levels of bisulfite can be detrimental to health. Hypochlorous acid (HOCl), which acts as bioactive small molecules, is crucial for maintaining normal biological functions in living organisms. Disruption of its equilibrium can lead to oxidative stress and various diseases. Therefore, it's essential to monitor the fluctuations of HOCl and HSO3− at cellular and in vivo levels to study their physiological and pathological functions. ResultsThis study constructed a novel NIR bifunctional colorimetric fluorescent probe using thienocoumarin-indanedione structures to identify hypochlorite (ClO−) and bisulfite (HSO3−). By using CSO-IO to recognize HSO3− and HOCl, two distinct products were generated, displaying green and blue fluorescence, respectively. This property effectively allows for the simultaneous dual-functional detection of HSO3− (LOD: 113 nM) and HOCl (LOD: 43 nM). SignificanceIn this work, the biocompatible molecule CSO-IO has been effectively designed to detect HOCl/HSO3− in living cells and zebrafish. As a result, the dual-functional fluorescent probe has the potential to be utilized as a molecular tool to detect HSO3− derived compounds and HOCl simultaneously within the complex biological system.

Direct lithography of CsPbCl3, CsPbBrxCl3−x and CsCdxPb1−xBr3 perovskite nanocrystals in organic media

The in-situ generation of perovskite nanocrystals within organic media using a femtosecond laser can greatly simplify the sample preparation process and save pulse energy. In this work, three perovskite nanocrystals (PNCs), CsPbCl3, CsPbBrxCl3−x, and CsCdxPb1−xBr3, were generated in situ within organic media using a femtosecond laser. These three perovskite nanocrystals emitted pure blue or blue-green fluorescence under ultraviolet (UV) irradiation. It was also found that by controlling the laser pulse energy, the CsCdxPb1-xBr3 nanocrystals transitioned from green to blue-green fluorescence. Furthermore, we have successfully realized the patterning of these three perovskite nanocrystals in organic media.

A novel biomass carbon dots ratiometric fluorescence probe coupled with molecularly imprinted polymer for selective detection of aflatoxin B1

The accumulation of Aflatoxin B1 (AFB1) in food and crops can pose significant hazards to human health. Consequently, there has been extensive attention paid to the development of a rapid and reliable method for detecting AFB1 in food. In this context, a ratiometric fluorescence probe (DE-MIPs) was prepared using dual-emission fluorescent carbon dots (CDs) coupled with the molecular imprinting technique for visual detection of AFB1. The dual-emission CDs consisted of red fluorescence biomass CDs (R-CDs) wrapped by molecularly imprinted cavities (R-CDs@MIPs), which served as a response signal, and pure blue emission biomass CDs (B-CDs) as a reference signal. Due to the specific quenching effect of AFB1 on red fluorescence while leaving blue fluorescence unaffected, there was a gradual decrease in the fluorescence intensity ratio (I670/I437) and a distinct shift in fluorescence color from red to blue as the concentration of AFB1 increased. The ratiometric probe demonstrated excellent linearity in detecting AFB1 within the concentration range of 0.1–200 ng/mL, with a detection limit (LOD) of 0.05 ng/mL. Furthermore, the practicality of DE-MIPs was validated by successfully detecting AFB1 in vegetable oil, with desirable recoveries from 89.0% to 107.4%. Furthermore, the integration of a smartphone as colorimetric reader enabled rapid AFB1 detection by capturing changes in fluorescence color. Overall, the development of this ratiometric probe offers a novel perspective for the facile and efficient identification of AFB1 in complex food samples.

A color-changed fluorescence sensor for pesticide triclopyr 2-butoxyethyl ester based on naphthalimide Schiff-base

Triclopyr 2-butoxyethyl ester is a usual pesticide, however, its excessive use might pose risks to environmental safety. The rapid, simple and in-situ detecting method for triclopyr 2-butoxyethyl ester is of great importance but rarely reported. In this work, the first fluorescent sensor for triclopyr 2-butoxyethyl ester was developed based on a derivative of n-butyl-1,8-naphthimide Schiff-base (NSB). NSB is a typical AIE molecule with good green fluorescence in DMSO-H2O (5:95). The green fluorescence of NSB was transferred into strong blue fluorescence on sensing triclopyr 2-butoxyethyl ester. The high selective detection of NSB for triclopyr 2-butoxyethyl ester was observed among 26 kinds of normal metallic ions and pesticide molecules. The detecting limitation was as low as 2.89 × 10−7 M. The sensing mechanism was confirmed as 1:1 stoichiometric ratio based on the multiple hydrogen bonding and hydrophobic interactions. NSB was applied on sensing triclopyr 2-butoxyethyl ester in real samples of wheat seedlings, sugarcane and broadleaf weedane and water samples, suggesting the excellent application potential on simple, rapid and in-situ detection of triclopyr 2-butoxyethyl ester in daily environments.

Rapid and on-site detection of bisulfite via a NIR fluorescent probe: A case study on the emission wavelength of probes with different quinolinium as electron-withdrawing groups

The emission of venenous sulfur dioxide (SO2) and its derivatives from industrial applications such as coking, transportation and food processing has caused great concern about public health and environmental quality. Probes that enable sensitivity and specificity to detect SO2 derivatives play a crucial role in its regulations and finally mitigating its environmental and health impacts, but fluorescent probes that can accurately, rapidly and on-site detect SO2 derivatives in foodstuffs and environmental systems rarely reported. Herein, a near-infrared (NIR) fluorescent probe (ZTX) for the ratiometric response of bisulfite (HSO3−) was designed and synthesized by regulating the structure of high-performance HSO3− fluorescent probe SL previously reported by us based on structural analyses, theoretical calculations and related literature reports. The Michael addition reaction between the electronic-deficient C=C bond and HSO3− destroys ZTX's π-conjugation system and blocks its intramolecular charge transfer (ICT) process, resulting in a significant fading of the fuchsia solution and the bluish-purple fluorescence turned light blue fluorescence. Fluorescent imaging of HSO3− in live animals utilizing ZTX has been demonstrated. The quantitative analysis of HSO3− in food samples using ZTXvia a smartphone has been also successfully implemented. Simultaneously, the ZTX-based test strips were utilized to quantificationally determine HSO3− in environmental water samples by a smartphone. Consequently, probe ZTX could provide a new method to understand the physiopathological roles of HSO3−, evaluate food safety and monitor environment, and is promising for broad applications.

Evaluation of non-anatomical characteristics for wood identification of six Korean oak species

Summary This study aimed to investigate and compare the non-anatomical characteristics of six Korean oak wood species (Quercus variabilis, Q. serrata, Q. mongolica, Q. dentata, Q. aliena and Q. acutissima) to provide identification keys and quality indices for the effective utilization of these species. Non-anatomical characteristics, including heartwood fluorescence, fluorescence and color of water and ethanol extracts, froth test, chrome azurol S test and burning splinter test, were evaluated according to the International Association of Wood Anatomists (IAWA) lists. None of the six Korean oak species displayed heartwood fluorescence or fluorescence in the ethanol extract. Only Q. serrata exhibited bright blue fluorescence in the aqueous extract. Each species showed distinct colors in both water and ethanol extracts, and the water extract of all species was darker than that of the ethanol extract. A weak positive reaction in the froth test was observed only for Q. serrata. Furthermore, Q. variabilis and Q. acutissima showed positive reactions to both heartwood and sapwood in the chrome azurol S test. The burning splinter test revealed that only Q. acutissima was transformed into charcoal. Thus, water extract fluorescence and color, ethanol extract color, froth test, chrome azurol S test, and burning splinter test can be used to identify the six Korean oak species.

One-Pot Synthesis of Silicon Quantum Dots-Based Fluorescent Nanomaterial and Its Application.

Conventionally obtained silicon quantum dots (Si QDs) generally suffer from the disadvantages of a cumbersome preparation process, large fluctuation in the quality of Si QDs, poor water solubility, and aggregation-caused quenching (ACQ) phenomenon. Here we report a facile one-pot strategy to synthesize a novel Si QDs-based fluorescent nanomaterial in which Si QDs are confined into dendritic mesoporous silica, named as SiQDs@DMSNs. The prepared SiQDs@DMSNs, with adjustable particle sizes ranging from 140 to 300 nm, emit blue fluorescence around 410 nm upon excitation by ultraviolet light at a wavelength of 300 nm. It is found that the addition of sodium salicylate (NaSAL) plays a crucial role in the in situ generation of Si QDs. The obtained SiQDs@DMSNs exhibit excellent fluorescence intensity, water solubility, and stability, facilitating easy surface modification, without being limited by the ACQ phenomenon. It is expected to be widely used in many fields such as biosensors, nanomedicines, in vivo imaging, fingerprint identification, and anticounterfeiting labels.

Physiological and molecular mechanisms of carbon quantum dots alleviating Cu2+ toxicity in Salvia miltiorrhiza bunge

Excessive Cu2+ is toxic to plants. Carbon quantum dots (CQDs) exhibit certain chelating properties towards heavy metals, and they also demonstrate antioxidant activities. To explore the mechanism for alleviating the Cu2+ toxicity of Salvia miltiorrhiza Bunge mediated by CQDs, CQDs that contained CC, CO, H–O, C–N and C–O functional groups with particle size less than 10 nm and that emitted blue fluorescence were prepared. S. miltiorrhiza seedlings were treated with 200 μM of Cu2+ and 500 mg/L of CQDs to relieve stress. Exogenous CQDs effectively restored plant phenotype; reduced Cu2+, H2O2 and malondialdehyde contents and restored total superoxide dismutase, peroxidase and catalase activities under Cu2+ toxicity. Simultaneously, an association network of Cu2+ transport-related and metabolic pathway genes of phenolic acids and terpenoids was established on the basis of cross-species transcriptome analysis. Combined with reverse transcription quantitative real-time polymerase chain reaction analysis, the potential molecular mechanism of CQDs, i.e. promoting phenolic acid biosynthesis to alleviate Cu2+ toxicity, was revealed by activating the expression of key enzyme genes of phenolic acid synthesis. This study provides a theoretical basis for Cu2+ pollution prevention and control in plants. It also laid a foundation for alleviating Cu stress by using CQDs in agricultural production.

A portable and versatile fluorescent platform for high-throughput screening of toxic phosgene, diethyl chlorophosphate and volatile acyl chlorides

Highly toxic phosgene, diethyl chlorophosphate (DCP) and volatile acyl chlorides endanger our life and public security. To achieve facile sensing and discrimination of multiple target analytes, herein, we presented a single fluorescent probe (BDP-CHD) for high-throughput screening of phosgene, DCP and volatile acyl chlorides. The probe underwent a covalent cascade reaction with phosgene to form boron dipyrromethene (BODIPY) with bright green fluorescence. By contrast, DCP, diphosgene and acyl chlorides can covalently assembled with the probe, giving rise to strong blue fluorescence. The probe has demonstrated high-throughput detection capability, high sensitivity, fast response (within 3 s) and parts per trillion (ppt) level detection limit. Furthermore, a portable platform based on BDP-CHD was constructed, which has achieved high-throughput discrimination of 16 analytes through linear discriminant analysis (LDA). Moreover, a smartphone adaptable RGB recognition pattern was established for the quantitative detection of multi-analytes. Therefore, this portable fluorescence sensing platform can serve as a versatile tool for rapid and high-throughput detection of toxic phosgene, DCP and volatile acyl chlorides. The proposed “one for more” strategy simplifies multi-target discrimination procedures and holds great promise for various sensing applications.