Blue Fluorescence Research Articles

A turn-on fluorescent probe based on pyrene for hydrazine detection

This paper presents a novel fluorescent probe (GNEM) based on a pyrene fluorophore, with dicarboximide as the recognition unit for hydrazine. The fluorescence probe can be synthesized in a simple one-step process. A highly selective and sensitive fluorescence turn-on GNEM synthesized. GNEM react with hydrazine through nucleophilic addition, leading to the deprotection of dicarboximide and the release of dicarboximide hydrazine, resulting in significant blue fluorescence. This enhanced emission is attributed to the intramolecular charge transfer (ICT) sensing mechanism of GNEM. In addition, detection of hydrazine in soil and water is conducted. By designing a simple detection device, the hydrazine content in the air can be quantitatively measured.

Rational design of long wavelength solid-state fluorescent carbon dots based on zirconium

As a new type of zero dimensional carbon based fluorescent nanomaterial, carbon dots (CDs) have been studied and developed for many years. Various methods have been explored to prepare multicolor CDs to achieve tunable fluorescence of CDs. However, synthesizing fluorescent CDs located in the long wavelength emission region remains a challenge. Here, through a simple one-step solvothermal method using anhydrous citric acid (CA) as the raw material, ZrCl4 as both a metal element dopant and an inorganic salt matrix, and NH3·H2O as nitrogen source, Zr-doped CDs (Zr-CDs) solution with long wavelength fluorescence emission and solid powders with blue fluorescence can be obtained simultaneously. By optimizing the usage of ZrCl4, the fluorescence emission of the prepared CDs red shifted from 596 nm to 650 nm. The introduction of Zr4+ can cause a significant redshift. In addition, based on the sensitivity of CDs to oxygen, they can be used for the detection of trace amounts of H2O2. When using metal-organic framework (MOF) to encapsulate the Zr-CDs which is in the solution, optically stable CD@MOF powders can be obtained, which can be used for the construction of red light-emitting diodes (LEDs), demonstrating its potential application in the field of solid-state lighting. The synthesis technology is expected to provide favorable technical support for the future development of CDs.

Supramolecular Chirality Achieved by Assembly of Small π-Molecules of Octahydrobinaphtols with Axial Chirality.

5,5',6,6',7,7',8,8'-Octahydro-1,1'-bi-2-naphthol (hbNaph) is an axially chiral molecule consisting of a smaller π-electronic system than that for 1,1'-bi-2-naphthol (BINOL). The absorption and circular dichroism (CD) bands of hbNaph appear in a shorter wavelength region below 310 nm, compared to those of BINOL, and its fluorescence is in the invisible UV region. However, increasing the concentration of hbNaph in solution up to 0.1 M results in its absorption edge gradually extending to longer wavelength, with a shoulder around 330 nm, and finally increasing to about 450 nm. At the same time, blue fluorescence is clearly observed, as well as a new CD band with the sign of the Cotton signals reversed from those obtained for dilute solutions. These results suggest that, at high concentrations, hbNaph forms chiral aggregates, in which π-electrons are delocalized over multiple molecules. To further understand how molecular axial chirality is transformed to supramolecular chirality, we attempted to construct aggregate models by simulating CD spectra using a time-dependent density functional theory. The only reasonable model obtained was that involving the counterclockwise R-enantiomer forming a clockwise helix, while the clockwise S-enantiomer forms a counterclockwise helix. We conclude, however, that, for such helixes, the most plausible model is densely packed and forms when the dihedral angle between the two phenol rings of hbNaph is acute, at around 75°, which reproduces the aggregate-induced CD sign inversion.

Fabrication of two-dimensional fluorescent stanene oxide nanosheets for the detection of cortisone in biofluids

The current work provides a new approach for the fabrication of two-dimensional blue fluorescent guanidine acetic acid templated stanene oxide nanosheets (GAA-SnO2 NSs) for selective determination of cortisone. The GAA-SnO2 NSs displayed blue fluorescence with a quantum yield of 21.16 %. Under excitation wavelength of 370 nm, the GAA-SnO2 NSs responded to cortisone by exhibiting noticeably decrease in the emission peak at 454 nm. The introduction of cortisone to GAA-SnO2 NSs significantly altered fluorescence emission, leading to achieve desirable linear relationship between the intensity ratio (I0/I) and the concentration (0.5–10 μM). The detection limit was found to be 41.74 nM for cortisone. The GAA-SnO2 NSs were decorated on paper for the development of embedded analytical probe for cortisone detection. Furthermore, the method was successfully demonstrated to assay cortisone in biofluid (plasma and urine) samples with good recoveries.

Supramolecular fluorescent organogel bearing 1,8-naphthalimide residue for the detection of Fe3+ through the gel-gel transformation

Supramolecular gels with metal ion-responsiveness are an important kind of smart materials due to their potential application in environmental monitoring. In this study, a novel l-glutamine-contained 1,8-naphthalimide derivative (NDG) has been designed and synthesized as an organogelator. NDG could self-assemble into stable supramolecular organogels in different kind of solvents, and the gelation process showed bright blue aggregation-induced emission (AIE). The self-assembly behavior, morphology and structure of NDG gels were thoroughly studied by various techniques. Interestingly, the obtained NDG organogel exhibited a fluorescence ‘‘turn-off’’ response for Fe3+ and formed a stable metallogel NDG-Fe3+. The detection limit (LOD) of NDG in diluted solution and gel states for Fe3+ was calculated to be 30.8 nM and 11.5 nM, respectively, indicating high sensitivity. In addition, the blue fluorescence emission of the gel system was restored in the presence of ascorbic acid and then quenched again with oxygen, which showed reversible fluorescence sensing effect and employed as a logic gate at the molecular level. Furthermore, xerogel-based film of NDG emitted strong blue emission and could be utilized as paper-based chemosensor to detect Fe3+. And also, the NDG gel had potential application in fluorescence display material.

Sensing and Microbiological Activity of a New Blue Fluorescence Polyamidoamine Dendrimer Modified with 1,8-Naphthalimide Units.

A novel second-generation blue fluorescent polyamidoamine dendrimer peripherally modified with sixteen 4-N,N-dimethylaninoethyloxy-1,8-naphthalimide units was synthesized. Its basic photophysical characteristics were investigated in organic solvents of different polarity. It was found that in these solvents, the dendrimer is colorless and emitted blue fluorescence with different intensities depending on their polarity. The effect of the pH of the medium on the fluorescence intensity was investigated and it was found that in the acidic medium, the fluorescence is intense and is quenched in the alkaline medium. The ability of the dendrimer to detect metal ions (Pb2+, Zn2+, Mg2+, Sn2+, Ba2+, Ni2+, Sn2+, Mn2+, Co2+, Fe3+, and Al3+) was also investigated, and it was found that in the presence of Fe3+, the fluorescent intensity was amplified more than 66 times. The antimicrobial activity of the new compound has been tested in vitro against Gram-positive B. cereus and Gram-negative P. aeruginosa. The tests were performed in the dark and after irradiation with visible light. The antimicrobial activity of the compound enhanced after light irradiation and B. cereus was found slightly more sensitive than P. aeruginosa. The increase in antimicrobial activity after light irradiation is due to the generation of singlet oxygen particles, which attack bacterial cell membranes.

Mn(II) Coordination Complex Loaded Hydrogel: Synthesis, Characterization, Fluorescence Properties, and Application in Treating Knee Arthritis.

Using a mixed-ligand approach, we successfully obtained two Mn(II)-based coordination compounds, namely [Mn2(L1)(TBIP)·H2O]n (1) and [Mn2(L2)(NPTA)·H2O]n (2) (where L1 and L2 are 1,4-bis(thiabenzimidazol-1-ylmethyl)benzene and 1,2-bis(thiabenzimidazol-1-ylmethyl)benzene, H2NPTA is 2-nitroterephthalic acid, and H2TBIP is 5-tert-butylisophthalic acid). Fluorescence performance testing of complexes 1 and 2 showed excellent green and blue fluorescence properties. Based on this, we further prepared HA/CMCS hydrogels using natural polysaccharides hyaluronic acid (HA) and carboxymethyl chitosan (CMCS) as raw materials and studied their internal structural characteristics using scanning electron microscopy. Using "Duhuo Jisheng Decoction" as a drug model, two metal gel scaffolds loaded with "Duhuo Jisheng Decoction" were prepared, and their potential for treating knee osteoarthritis was evaluated.

A Phenanthrenequinone-Based Ratiometric Fluorescent Probe for Rapid Detection of Peroxynitrite with Imaging in Osteoblast Precursor Cells.

In the current situation, peroxynitrite (ONOO-) is drawing the increasing attention of researchers for its pivotal role in diverse pathological and physiological processes on grounds of robust oxidation and nitrification. Herein, we have successfully designed and synthesized a phenanthrenequinone benzyl borate-based chemosensor for fast and selective detection of ONOO-. The probe PTDP itself had an orange fluorescence, which was changed to strong blue fluorescence upon the addition of ONOO-, indicating the ratiometric response of the probe. This is so because of the cleavage of the benzyl boronate-protecting group of PTDP upon the addition of ONOO- with simultaneous releasing of pyridinyl-based chemosensor PPI. The PTDP showed outstanding performance in the various photophysical studies such as good selectivity, excellent sensitivity with a very low detection limit of 2.74 nM, and a very fast response time (<15 s). Furthermore, for practical applicability, it was successfully applied in the ratiometric detection of ONOO- in osteoblast precursor cells.

Multistimuli-responsive tetraphenylethene molecular switches displaying acid-sensing, photochromism, and metal ion detection properties

Aggregation-induced emission (AIE) smart materials with multiple stimulus responses have a wide range of applications. Here, a series of tetraphenylethylene (TPE) derivatives, TPEN1S3, TPEN2S2, TPEN3S1 were synthesized. The sensitivity of molecules to pH, UV light, and metal ions could be effectively tuned by modulating the ratio of amino to sulfonic acid groups. These TPE derivatives exhibited yellow emissions in aggregated state due to the AIE characteristics. Acidification conversion of the NH2 groups to NH3+ for three molecules induced the suppression of intramolecular charge transfer (ICT), which resulted in a 72–102 nm blue shift in the fluorescence emission. Solid powders of these compounds exhibited reversible protonation-deprotonation process induced by HCl/NH3·H2O vapor. TPEN1S3, TPEN2S2 and TPEN3S1 in solution could be photoactivated and emit blue fluorescence (ca. 482 nm, 475 nm, and 480 nm). Photocyclo-dehydrogenation reactions of compounds were triggered by the UV irradiation and the maximum cyclization ratios could reach 12%, 66%, and 50%, respectively. Protonation of NH2 group significantly enhanced the rate of the photocyclo-dehydrogenation reaction. Furthermore, the solid powders of protonated compounds displayed pronounced reversible photochromic properties due to the generation of cyclization intermediate. In addition, TPEN1S3, TPEN2S2, and TPEN3S1 exhibited responsiveness to a diverse range of metal ions. Specifically, the TPEN2S2 molecule functioned as a photo sensor for Li + ions with a blue shift in fluorescence emission. The limit of detection (LOD) value was 0.679 μM. These multiple stimuli-responsive AIE molecules hold significant potential for use in advanced photo-controlled patterning, encryption applications, and the detection of metal ions.

Methotrexate-loaded manganese nitrogen dual-doped carbon quantum dots as targeted nano drug-delivery system for potential use in cancer theranostics

The present work highlights manganese nitrogen dual-doped carbon quantum dots as a novel pH-sensitive drug delivery system, for the delivery of methotrexate, targeted to cancer cells, along with the simultaneous real-time monitoring of cellular uptake and drug delivery using fluorescence imaging. Briefly, methotrexate was conjugated with the as-prepared quantum dots to form a nano drug-delivery system through non-covalent interactions. The developed material demonstrated drug loading efficiency of ∼91 % and a very efficient (∼80 %) pH-responsive drug release at pH 5.5. The cytotoxic properties of the developed nano-platform viz. manganese, nitrogen dual-doped carbon quantum dots, and methotrexate conjugated manganese nitrogen dual-doped carbon quantum dot complex were evaluated in cancer cells (HeLa) and healthy human cells (L929). The conjugated complex demonstrated excellent biocompatibility, high cellular uptake, and a substantial drug release. Healthy human cells were largely unaffected when incubated with both the MTX-conjugated Mn-, N-CQD and the free drug. Additionally, in vitro, the fluorescence bio-imaging potential of the developed nano-platform was also assessed, where the unconjugated (free) quantum dots exhibited better blue fluorescence imaging potential compared to that of the conjugated complex, which can be attributed to fluorescence quenching due to the presence of methotrexate. This study highlights the manganese nitrogen dual-doped carbon quantum dots capable of pH-responsive methotrexate drug delivery selective to cancer cells. The combination of drug delivery and fluorescence imaging creates an efficient platform for targeted therapy, allowing very precise drug release while accessing non-invasive monitoring of therapeutic response. This method holds a great promise for advancing cancer treatment strategies, with the potential for increased efficacy and decreased side effects.

Green synthesis of yeast cell wall-derived carbon quantum dots with multiple biological activities

HypothesisYeast cell walls are a sustainable biomass source containing carbon and other elements like phosphorus. Converting cell walls into valuable nanomaterials like carbon quantum dots (CQDs) is of interest. ExperimentsCell walls from Saccharomyces cerevisiae were hydrothermally treated in 0.5 M H2SO4 to produce CQDs. Multiple analytical techniques were utilized to confirm phosphorus-doping (P-CQDs), characterize the fluorescence properties, determine quantum yield, and evaluate the sensing, antimicrobial, photocatalytic, and antioxidant capacities. FindingsA successful synthesis of P-CQDs was achieved with strong blue fluorescence under UV excitation, 19 % quantum yield, and excellent stability. The P-CQDs showed sensitive fluorescence quenching in response to ferric ions with a 201 nM detection limit. Antibacterial effects against Escherichia coli and Staphylococcus aureus were demonstrated. P-CQDs also exhibited dye degradation under sunlight and antioxidant activity. So, the prepared P-CQDs displayed promising multifunctional capabilities for metal ion detection, disinfection, and environmental remediation. Further research is required to fully realize and implement the multifunctional potential of P-CQDs in real-world applications.

Multicolor lignin-derived carbon quantum dots: Controllable synthesis and photocatalytic applications

The cost-effective, high-performance fabrication of multicolor carbon quantum dots (CQDs) from biomass is critical to its prospective application. Lignin, with its phenylpropane structural unit and abundant functional groups, is a reliable precursor for the formation of high-quality CQDs. Here, CQDs from renewable lignin were successfully prepared via a two-step method, and controllable preparation of CQDs to emit blue, green, yellow, and red fluorescence was achieved by adjusting the N-containing acid dopant. The investigated fluorescence mechanism revealed that the energy bandgap of the lignin-derived CQDs decreased and the emission wavelength redshifted as the graphitization degree and number of C=O surface groups increased. The light absorption region of the Bi7O9I3 photocatalysts modified with blue CQDs to red CQDs gradually broadened in the redshifted direction. In particular, compared with pure Bi7O9I3 (70.9%), the red CQDs-modified Bi7O9I3 (RCQD/BOI) can remove 94.7% of antibiotics within 60 min of light irradiation. The superior photocatalytic activity of RCQD/BOI is attributed to the established internal electric field that accelerates charge transfer and effectively separates the photogenerated e+-h+ inside Bi7O9I3. Furthermore, through radical capture, electron paramagnetic resonance (EPR) experiments validated the photocatalytic mechanism of RCQD/BOI and revealed that h+, O2− and OH are key reactants in the photocatalytic degradation process. This promising and sustainable approach for the synthesis of multicolor lignin-derived CQDs opens the avenue of high-value utilization of biomass resources, green production of CQDs nanomaterials, and efficient development of CQDs-based photocatalysts for wastewater treatment.

A triphenylamine-thiophene-based fluorescent probe for the dual-channel detection and imaging of hypochlorite and viscosity in live cells

In this research, a new triphenylamine-thiophene-based fluorescent probe, denoted as TTD, was constructed for simultaneous detection of viscosity and hypochlorite (ClO−) with dual-response signals. The hindrance of intramolecular bond rotation within TTD in a high-viscosity environment turns on the probe’s emission at 600 nm, resulting in strong red fluorescence. Simultaneously, the oxidation of the imine moiety to the aldehyde product, induced by ClO−, leads to substantial emission enhancement at 460 nm, generating vivid blue fluorescence. As a result, TTD can be employed for the dual-color visualization of changes in viscosity and ClO− levels, demonstrating good selectivity. TTD has been applied to image the elevated level of viscosity induced by nystatin, rotenone or erastin as well as exogenously introduced ClO− in living cells. Importantly, from two independent emission channels, TTD is capable of monitoring both ClO− and viscosity fluctuations within live cells during the inflammatory response triggered by lipopolysaccharide (LPS).

Evaluation of Chemical and Biological Properties of Biodegradable Composites Based on Poly(3-hydroxybutyrate) and Chitosan.

In this study, composite films and scaffolds of polyester poly(3-hydroxybutyrate) and polysaccharide chitosan obtained via a simple and reproducible blending method using acetic acid as a solvent were considered. The degradation process of the films was studied gravimetrically in a model biological medium in the presence of enzymes in vitro for 180 days. The kinetics of weight reduction depended on the amount of chitosan in the composition. The biocompatibility of the films was evaluated using the Alamar blue test and fluorescence microscopy. The materials were non-cytotoxic, and the addition of poly(3-hydroxybutyrate) to chitosan improved its matrix properties on mesenchymal stem cells. Then, the 3D composites were prepared by freeze-drying. Their structure (using SEM), rheological behavior, moisture absorption, and porosity were investigated. The addition of different amounts of chitosan allowed us to vary the chemical and biological properties of poly(3-hydroxybutyrate) materials and their degradation rate, which is extremely important in the development of biomedical poly(3-hydroxybutyrate) materials, especially implantable ones.

Green one-step synthesis of N-doped carbon quantum dots for fluorescent detection of lemon yellow in soft drinks

A new fluorescent sensor for the determination of lemon yellow was developed based on nitrogen-doped carbon quantum dots (N-CQDs), which were prepared via a hydrothermal method with dried pomelo peel and L-tyrosine. The N-CQDs exhibited the blue fluorescence with a quantum yield of 28 %. The sensing principle of N-CQDs was quenched by lemon yellow via static quenching. The potential interfering substances showed no influence on the detection of lemon yellow. The limit of detection was 0.023 mg/L and lower than that of national standard. Furthermore, the synthesized N-CQDs have been successfully applied to the measurement of lemon yellow in real samples. Hence, the N-CQDs would be a promising sensor in food analysis.

Two Mixed-Ligand Co(II) Complexes as Luminescent Materials and Loaded with Temozolomide-gel Particles in Nursing Against Glioma.

By employing a mixed-ligand strategy, we synthesized two new coordination polymers (CPs) featuring Co(II): {Co(H2L)(bib)]·2H2O}n (1) and {Co(L)(bib)2]·2H2O}n (2), where H4L represents 5-(3,5-dicarboxybenzyloxy) isophthalic acid, and bib denotes 1,4-bis(1-imidazolyl)benzene. These CPs were obtained through the reaction of H4L, a flexible carboxylic acid ligand, with Co(NO3)2·6H2O in various solvent mixtures, along with the N-donor co-ligand bib. Complexes 1 and 2 are formed through distinct coordination modes, resulting in their distinct structural features and excellent fluorescent properties. Based on ligand-centered fluorescence emission and the blue shift (CP 1) along with red shift (CP 2) characteristics, both complexes show promise for applications in fields such as blue fluorescence sensing materials and luminescent materials. After successfully synthesizing two CPs, CP 1 was chosen as the carrier for loading temozolomide (TMZ). Subsequently, leveraging the unique advantages of hydrogels, we developed a novel metal gel formulation loaded with TMZ. The inhibitory effect of this formulation on the growth of glioblastoma was evaluated. Our results demonstrate a significant suppression of glioblastoma cell proliferation by this system, providing an effective avenue for glioblastoma treatment.

An easy-to-prepare AIE fluorescent sensor based on bis-(naphthyl acetyl)-hydrazone for selectively relay detection of Fe3+, F− and H2PO4−

It is important to identify and detect ions as they are essential in the life system. The development of fluorescent sensors with easy-to-prepare and high sensitivity has always been a topic of widespread concern. Herein, an easily prepared fluorescent sensor based on bis-(naphthyl acetyl)-hydrazone (BNH) was successfully synthesized. Remarkably, the BNH exhibits blue aggregation-induced emission (AIE) fluorescence. The blue fluorescence of BNH was quenched by the addition of Fe3+, and then enhanced by the addition of F− and H2PO4−, while the fluorescence opened by F− was quenched again by Al3+. The LODs (limits of lowest detection) of BNH for Fe3+ is 4.46 × 10−7 M. This study provides a simple and feasible AIE fluorescence sensor synthesis method for efficient and sensitive detecting Fe3+, F− and H2PO4−.

A metal-organic framework and quantum dot-based ratiometric fluorescent probe for the detection of formaldehyde in food.

Agreen and sensitive ratio fluorescence strategy was proposed for the detection of formaldehyde (FA) in food based on a kind of metal-organic frameworks (MOFs), MIL-53(Fe)-NO2, and nitrogen-doped Ti3C2 MXene quantum dots (N-Ti3C2 MQDs) with a blue fluorescence at 450nm. As a type of MOFs with oxidase-like activity, MIL-53(Fe)-NO2 can catalyze o-phenylenediamine (OPD) into yellow fluorescent product 2,3-diaminophenazine (DAP) with a fluorescent emission at 560nm. DAP has the ability to suppress the blue light of N-Ti3C2 MQDs due to inner filter effect (IFE). Nevertheless, Schiff base reaction can occur between FA and OPD, inhibiting DAP production. This results in a weakening of the IFE which reverses the original fluorescence color and intensity of DAP and N-Ti3C2 MQDs. So, the ratio of fluorescence intensity detected at respective 450nm and 560nm was designed as the readout signal to detect FA in food. The linear range of FA detection was 1-200µM, with a limit of detection of 0.49µM. The method developedwas successfully used to detect FA in food with satisfactory results. It indicates that MIL-53(Fe)-NO2, OPD, and N-Ti3C2 MQDs (MON) system constructed by integrating the mimics enzyme, enzyme substrate, and fluorescent quantum dots has potential application for FA detection in practical samples.

A multifunctional fluorescent probe for tracking SO2 fluctuation between mitochondria and endoplasmic reticulum in living cells and zebrafish

The sulfur dioxide (SO2)-associated dynamic interplay between mitochondria (Mito) and endoplasmic reticulum (ER) is essential for intracellular homeostasis regulation. Visualizing such organelles interactions can help elucidate basic pathogenesis. However, it is hampered by challenges in developing quick response, remarkable sensitivity and high selectivity within a single fluorescent probe for simultaneous and distinct tracking of their crosstalk. Herein, we engineered a SO2-responsive Mito-ER dual-targeted fluorescent probe (MER-SO2) for dual-color visualization of Mito (red fluorescence) and ER (blue fluorescence) in living cells and in vivo. Under physiological conditions, the benzopyranium cationic structure in MER-SO2 could target Mito, following fast response to SO2 (5 s), which converted to a neutral structure, targeting to ER emitting blue fluorescence via functional fluorescence conversion. Probe MER-SO2 also displayed high sensitivity, favourable specificity (against 24 types of non-targeting analytes). Importantly, such a high-performance probe allowed in-situ and dynamic tracking of Mito-ER interactions in Mito-dysfunction living cells and zebrafish via co-localization. Furthermore, the probe enabled accurate detection of sulphite in food with recoveries rate (96–100%), and incorporation into an agarose-based portable device for SO2 assay. Taken together, this initial SO2-responsive probe represents a promising tool for discriminative tracking of functional Mito-ER interactions in living systems and holds great potential in many other fields such as food engineering.

High Efficiency Triplet‐Triplet Fusion Blue Fluorescence OLEDs by introducing a “Hot Exciton” Efficiency Enhancement Layer

AbstractTriplet‐triplet fusion (TTF) can effectively transfer triplet excitons to singlet energy level and exhibit good stability, making it widely used in blue fluorescence organic light emitting diodes (OLEDs) for practical application. However, the efficiency of the resulting blue fluorescence OLEDs is still fundamentally limited. Herein, this work introduces a hot exciton efficiency enhancement layer (EEL) between TTF emissive layer and electron‐transporting layer for the first time. It can be found that the triplet excitons are greatly utilized by the efficient hot exciton EEL. As a result, a high efficiency blue fluorescence OLED is successfully developed, exhibiting a maximum external quantum efficiency of 15.2% at the luminance of 4068.0 cd m−2, achieving a breakthrough in the efficiency of blue TTF‐OLEDs. Transient electroluminescence (TrEL) measurements and detailed theoretical calculations confirm the key role of the effective hot exciton process from the high‐lying triplet energy level (Tn, n ≥ 2) to S1 via a reverse intersystem crossing (hRISC). Furthermore, the device lifetime is also greatly improved owing to the fast and efficient hRISC in the introduced hot exciton EEL. This work has important guiding significance for constructing high efficiency blue fluorescence OLEDs by introducing hot exciton molecules.