Black Tilapia Research Articles

Evaluation of black tilapia (Oreochromis niloticus) hatchery in UPT-Perikanan Budidaya Air Tawar (PBAT) Sukorejo, Malang

Abstract Black tilapia is a freshwater fish that has become a leading commodity in the UPT-PBAT (Unit Pelayanan Teknis-Pengembangan Budidaya Air Tawar) Sukorejo Freshwater Aquaculture Fisheries with an optimal selling price. The purpose of this study was to evaluate the hatcheries activity performance in UPT-PBAT Sukorejo. The method in this research used active participation and descriptive methods. The data collection method used was from primary and secondary data. Primary data taken in this study were fertilization rate, hatching rate, and survival rate while secondary data was from literature comparison and asking directly to the staffs of UPT-PBAT Sukorejo. The results of hatchery performance showed fertilization rate of 95%, hatching rate of 98.6%, and survival rate of 93%. Hatching was carried out naturally using a 1:3 ratio (50 male fish and 150 female fish per pond). The research results indicated that hatchery activity in UPT-Freshwater Aquaculture (PBAT) Sukorejo, Malang showed good performance. However, water quality (ammonia) needs more attention to prevent negative effect in the next aquaculture cycle activity since this study found the ammonia value reached minimum tolerable value. Ammonia that exceeds the limit can cause mortality to black tilapia. Changing water is important to avoid mortality due to high ammonia content.

Effect of Crossbreeding on Fecundity, Growth Performance, and Heterosis of Black Tilapia, Red Tilapia, and Mozambique Tilapia Reared in Earthen Ponds in West Java, Indonesia

Graphical Abstract Highlight Research The crossbred performance of the four tilapia strains showed diverse heterosis values, with some being better, worse, or the same as their parents. Crossbred of black tilapia produced the best growth and fecundity performance. A hybrid of Mozambique tilapia and black tilapia showed the highest mid-parent heterosis value on growth traits but produced negative heterosis on fecundity, biomass, and survival traits. Crossbred of black tilapia and Mozambique tilapia have the potential to be used as candidates for cultivation and performance improvement through selection, although there are depressions and crossover advantages that are not prominent. Abstract Increased tilapia production is challenged by genetic decline. Hybridization efforts for performance improvement through a selection of the best parent and strain pairs were a promising option. The objective of this study was to evaluate the crossing of black tilapia, red tilapia, and Mozambique tilapia against the performance of fecundity, growth, and survival and estimate the value of heterosis. The experimental design used a completely randomized design with 3 replications with the treatment of different populations of crosses. The rearing activities were carried out in earthen ponds for 150 days with a stocking density of 10 fish/m2. The parameters observed included egg fecundity, growth, survival, and the value of heterosis. The results showed that the fecundity and growth values of crossbred black tilapia were significantly higher than others (p<0.05). The highest survival rate was shown by crossing pure strains of red tilapia. The hybrid of Mozambique tilapia and black tilapia (♀ MJ x ♂ NW) showed the highest mid-parent heterosis value on growth characters but produced negative heterosis on characters, fecundity, biomass, and survival. Overall, the crossbred of black tilapia (♀BS x ♂ NW) performed better than the inbred strains, with positive mid-parent heterosis in all characters measured. These results indicate that crossbreeding has the potential to be used as a candidate for cultivation and performance improvement through selection, although there were depressions, and the superiority of the crosses was not prominent.

Effects of Acid Pre-Treatment on Removal of Organic and Inorganic Substances from Black Tilapia Bones and Scales

Fish bones and scales are good sources of gelatin. However, high mineral content in bones and scales often lead to low quality gelatin with high percentage of ash. Therefore, pre-treatment with acid prior to gelatin extraction is necessary. Black tilapia (Oreochromis niloticus) bones and scales were subjected to Hydrochloric (HCl) acid of 0.6 M to 1.4 M for 0 to 32 hr. The suitable pre-treatment conditions were determined using percentage of ash removed and loss of protein from the samples. Results obtained indicate, 99.86 % and 99.84 % of ash can be removed from bones and scales, with 1.4 M HCl (32 hr) and 1.0 M HCl (10 hr), respectively. Further analysis with UV-Vis revealed that, excessive protein breakdown starts to occur at 1.0 M (32 hr) and 0.6 M (12 hr), in bones and scales, respectively. The data suggest using pre-treatment conditions lower than the above to avoid protein loss which may lead to low gelatin yield.

Comparing the Behavior and Growth of Red and Black Tilapia in the Same Pond

Comparing the Behavior and Growth of Red and Black Tilapia in the Same Pond

Toxicity study and effect of protein hydrolysates derived from black tilapia (Oreochromis placidus) on hypertensive induced mice

Protein hydrolysates are specific protein fragments, produced during enzymatic hydrolysis and can exert various biological functions. The present study investigated the acute toxicity study of protein hydrolysates derived from black tilapia (Oreochromis placidus) and their effect on hypertensive-induced mice. Protein hydrolysates were produced from black tilapia protein isolate through enzymatic hydrolysis. A single dose of protein hydrolysates at 2000 mg/kg of body weight was administered and any changes were observed for 14 days. During 14 days, there were no significant changes in liver enzyme content and kidney function content, adverse clinical signs and mortality. The effect of the administration of protein hydrolysates in hypertensive-induced mice was studied. The body weight, blood pressure, full blood count {red blood cell (RBC), white blood cell (WBC) and platelets} were observed. Hypertensive mice (systolic blood pressure; 161±3 mm/Hg) were fed with pellet consisting of protein hydrolysates at different concentrations as well as carvedilol and combination of carvedilol and protein hydrolysates and systolic blood pressure was monitored. After 28 days, there was a significant reduction of blood pressure in the group of carvedilol (107±4 mm/Hg), 5% protein hydrolysate (119±1 mm/Hg), 10% protein hydrolysate (123±1 mm/Hg), 20% protein hydrolysate (115±2 mm/Hg) and 10% protein hydrolysate in combination with carvedilol (103±2 mm/Hg). Red blood cells significantly increased in hypertension mice but no significant changes in white blood cells and platelets after 28 days. Administration of protein hydrolysates reduced red blood cell levels similar to control group. A significant increase in body weight was observed in hypertension mice administered with protein hydrolysates at different concentrations as well as carvedilol and a combination of carvedilol and protein hydrolysates compared to control group and hypertensive group. Studies on any changes in liver enzyme level, kidney function level and angiotensin converting enzyme (ACE) activity on blood serum were carried out. Results showed that treatment with protein hydrolysates on hypertensive-induced mice reduced liver enzyme levels (ALP, ALT and GGT) and kidney function levels (Na+, K+ and Cl-) to normal levels. ACE enzyme activity of hypertensive-induced mice in the serum was also reduced similar to control mice after being treated with protein hydrolysates.

Growth response and estimating heritability of synthetic base population (F0) of red tilapia (Oreochromis spp.) through family selection

Red tilapia (Oreochromis spp.) is widely cultivated because it has high economic value. However, at the same age, the growth and thickness of red tilapia flesh are lower than that of black tilapia, which is necessary to improve genetics, one of which is a breeding program. The purpose of this study was to evaluate growth performance and estimate the value of the genetic parameter of the synthetic based population (F0) of red tilapia. Spawning was done naturally using happa with 30 males and 90 females. The number of families produced was 30 families, consisting of male and female sub populations. Enlargement activities were carried out for 90 days with a stocking density of 7 fish/m2 and a frequency of feeding 2 times a day. The estimated heritability was 0.26±0.07 with a response to selection of 18.52 g (male) and 0.35±0.09 with a response to selection of 20.69 g (female). Based on these parameters, the selection process for the next generation can still be done by increasing the number of families.

Alteration of Physico-mechanical Properties of Black Tilapia Scale Gelatins using UVA and UVC Irradiation

Fish gelatin is abundant, relatively low cost and biodegradable. However, their inferior mechanical and rheological properties make them less competitive compared to mammalian gelatins. Hence, the effects of Ultraviolet (UV) irradiation on the properties of scale gelatins were analyzed. Gelatins were extracted from black tilapia scale via thermal extraction method. The gelatins were then subjected to Ultraviolet-A (UVA) and Ultraviolet-C (UVC) irradiation for 0.5 to 2.5 h and the changes in gel strength, viscosity, and melting temperature were observed. Results obtained show a marked increase in the gel strength and viscosity of the gelatins. However, the effects on the melting temperature are minimal. Treatment with UVA and UVC improved the gel strength of the gelatins up to 5.12 ± 0.22 N and 4.75 ± 0.09 N, respectively. Further analysis using Fourier Transform Infrared (FTIR) Spectroscopy showed crosslinking formation in the polypeptide chains induced by UV irradiation. UVA was found to be more effective in enhancing the properties of scale gelatins compared to UVC. In general, UV-irradiated scale gelatins showed excellent properties compared to the commercial bovine gelatin. Results indicated the prospects of employing UV treatment in enhancing the properties of fish gelatin.

Characterization of the Halal Natural Hydroxyapatite Extract from Black Tilapia Fish Scale

Extraction of the natural halal hydroxyapatite (HAp) from fish scale (Black Tilapia) was produced by calcining of raw fish scale at 600 °C and 800 °C. The produced powders were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM) equipped with an Energy Dispersive Spectroscopy, (EDX). The crystallite size, cell parameters, strain and crystallinity of the raw fish scale and derived HAp calcined powder were observed from the XRD results. By increasing the calcination temperature, the average crystallite size HAp amount increased and close to the pure HAp (JCPDS: 9-432). SEM analysis showed the raw fish scale surface morphology contains collagen fiber compared with boiled and after calcination of the powder samples. The calcined fish scale powders at 800°C morphology was discovered embraced of hexagonal fragments form. The EDX investigation exposed that the fish scale from HAp powder calcined at temperature 600 °C contain a high amount of magnesium and sodium compared to the powder that was calcined at 800 °C.

Extraction and Characterization of Natural Hydroxyapatite from Black Tilapia Fish Bone for Biomedical Applications

Hydroxyapatite is known as HAp, Ca10(PO4)6(OH)2, commonly used in orthopedics since it resembles the inorganic portion of human bone. Calcium, Ca and phosphorous, P are the main components of HAp, with Ca/P molar ratio of 1.67 capable of promoting bone growth. Large quantities of by-product waste from fisheries factories have a negative effect on the environment. Hence, this research was carried out to obtain biological natural HAp derived from fishery factory waste black tilapia fish bones. As a result, fish bone can be a low-cost source of HAp and important contributions in biomedical applications. To remove meat and other impurities, black tilapia fish bones were boiled at 100 °C followed by milling to produce a fine powder. The powder was calcined at 600 °C and 800 °C for 3 hours. Characterizations were done by using X-ray diffraction (XRD) for mineralogy, Scanning Electron Microscopy (SEM) for morphology and Energy Dispersive Spectroscopy, EDX for element analysis. The XRD results showed the existence of derived HAp, which was consistent with standard HAp. The raw samples tended to have denser and less porous microstructures than calcined samples were shown in SEM results. EDX results showed the chemical composition of Ca and P with present of magnesium, Mg and sodium, Na as their traces elements. The samples' Ca/P molar ratio was found to be higher than the stoichiometric HAp, >1.67. Thus, the findings show that producing calcium and phosphorus from fishery wastes is a viable option for biomedical applications with the present of trace elements to help accelerates bone growth.

Effect of the Calcination Temperature on the Properties of Hydroxyapatite from Black Tilapia Fish Bone

Hydroxyapatite, also known as HAp, Ca10(PO4)6(OH)2 is a naturally present form of calcium phosphate, which make up a large portion of inorganic components in human bones. Because of its near resemblance in structure to natural bone, it has been commonly used in orthopaedic applications. The ecosystem is negatively impacted by large amounts of by-product waste from fisheries factories. Thus, the aim of this study is to extract the HAp from black tilapia fish bones (BTFB) from a fish fillet factory. Fish bone can serve as a low-cost source of HAp and contribute significantly to biomedical applications. The BTFB was calcined for 3 hours at 700 °C and 900 °C, respectively. The results of X-ray diffraction (XRD) revealed the presence of derived HAp, which matched data from the Joint Committee on Powder Diffraction Powder Standard (JCPDS). For functional group analysis, Fourier Transform Infrared Spectroscopy (FTIR) was used, and the organic compounds were removed throughout the calcination process according to the spectra. The chemical composition of Ca and P was revealed by Energy Dispersive Spectroscopy (EDX), with traces of magnesium, Mg, and sodium, Na present. In the BTFB samples, the Ca/P molar ratio was determined to be 1.67 which is the stoichiometries HAp. These findings have potential as a biomaterial for biomedical applications.

Sample Preparation of the Natural Source Hydroxyapatite (HAp) derived from Black Tilapia Fish Scales

Hydroxyapatite (HAp) is a biomaterial with the chemical formula Ca10(PO4)6(OH)2. Normally, it was used and applied in biomedical applications, cooking recipes and healthy food products. Due to this, it has attracted the researcher to work on HAp synthesis and extraction. In this study, the hydroxyapatite was extracted from fresh water Black Tilapia Fish Scales (BTFS) was analysed. The crystallinity of the HAp was characterized by using an X-ray diffractometer (XRD) whereas the Fourier transform infrared (FTIR) analysis was used to characterize the presence of HAp from the samples. Scanning electron microscopy (SEM) provided with an Energy Dispersive Spectroscopy, (EDS) was used to investigate the morphology and element of the hydroxyapatite powders. The BTFS samples were heated at 100 °C to composed and eliminated the remaining meat and other impurities. A mechanical crusher was applied to make a miniscule and fine hydroxyapatite powder using a thermal calcination technique using a furnace. The BTFS samples powder were calcined at a temperature of 900 °C for 3 hours correspondingly. The XRD spectra findings, uncovered the existence of the obtained BTFS HAp are articulate with Joint Committee on Powder Diffraction Powder Standard data (JCPDS) from the library. From the SEM results, HAp powder presence in hexagonal shape. The findings show the potential of producing high-value products from fishing waste, such as HAp with Ca/P ratio 1.74, for biomedical applications.

Adsorption efficiency of hydroxyapatite synthesised from black tilapia fish scales for chromium (VI) removal

Water pollution is a major problem that impacts financial growth and socio-ecological sustainability of a country as well as health of the population. The presence of toxic heavy metals in wastewater such as chromium (Cr) (VI), copper, arsenic, lead, mercury, cadmium, etc. may lead to serious health issues. Therefore, this paper aims to synthesise natural hydroxyapatite (HAp) from black tilapia fish scales and investigate its efficiency as adsorbent for Cr (VI) removal. In this study, the black tilapia fish scales were soaked and washed thoroughly with distilled water to eliminate impurities such as dust and other particles, then dried in the oven before being alkaline treated for 1 h with a 50% NaOH at 100 °C. High purity HAp with an irregular rod-shape was successfully synthesised. The effectiveness of HAp from tilapia fish to remove Cr (VI) was tested at various initial concentrations (30–70 mg/L) and HAp dosages ranging from 2 to 6 g. The results discovered that the synthesised adsorbent had a substantial impact on the removal effectiveness of the Cr (VI) for both manipulated parameters; initial concentration and Cr (VI) pH. The optimum result for Cr (VI) removal rate was achieved at 61.43% at 60 min by using 6 g of HAp. Freundlich isotherm model shows greater suitability with higher R2 = 0.995. Meanwhile, pseudo-second-order model is favourable for the adsorption process occurred in this study owing to its higher correlation coefficients. According to the findings of this study, black tilapia scale waste can be transformed into an effective adsorbent for Cr (VI) removal in wastewater treatment. This includes industries that may be exposed to Cr (VI), such as welding, coatings, plating, and textile production that use chromium-containing metals.

Red Hybrid Tilapia (Oreochromis spp.) Broodstock Development Programme in Malaysia: Status, Challenges and Prospects for Future Development

The red hybrid tilapia (Oreochromis spp.) and the black tilapia, including the genetically improved farmed tilapia (GIFT) (Oreochromis niloticus (Linnaeus, 1758)) collectively contributed to about 30.7 % of the total freshwater aquaculture production in Malaysia in 2018. The red hybrid tilapia is the most important, accounting for 94 % of total tilapia production. Among the major problems encountered in tilapia farming is the inconsistent supply of quality and adequate seeds, which could be solved through systematic breeding programmes. Among the major problems encountered in tilapia species farming is the inconsistent supply of quality and adequate seeds, which could be solved through systematic breeding programmes. This paper discusses the status of the red hybrid tilapia breeding programme in Malaysia, including issues and future perspectives. In brief, the first systematic breeding programme was initiated by the Fisheries Research Institute (FRI) in 2008. Selected founder stocks from Malaysia, Taiwan and Thailand were used to establish a base population for the programme. In this programme, the combined selection was practised which produced six generations of selection and successfully improved 12.5 % of harvest body weight per generation. The 6th generation was used as one of the founder stocks, apart from FRI Glami Lemi, Negeri Sembilan and Pahang populations to improve resistance to Streptococcus agalactiae. In 2017, the scope of the programme was expanded to include molecular tool in identifying markers for growth. Ten SNP markers associated with high growth performance traits were discovered. The provision of better breeding stocks for the aquaculture industry and the development of safe and productive operations are expected to result in more stable fish production and an improved income for farmers.

Organic acid composition and consumer acceptability of fermented fish produced from black tilapia (Oreochromis mossambicus) and Javanese carp (Puntius gonionotus) using natural and acid-assisted fermentation

Ikan pekasam is an example of traditional fermented food products in Malaysia, prepared from freshwater fish such as black tilapia (Oreochromis mossambicus) and Javanese carp (Puntius gonionotus), salt and ground roasted rice, which is added as the only source of carbohydrate. However, some producers add tamarind (Tamarindus indica) pulp and/or dried slices of Garcinia atroviridis to assist the fermentation process. In this study, there was no correlation detected between pH value, titratable acidity (% lactic acid), salt concentration, organic acid composition and the consumer acceptability of ikan pekasam commercial samples made of black tilapia as well as Javanese carp. The organic acid composition in both natural and acid-assisted fermented ikan pekasam from black tilapia and Javanese carp were compared. The results obtained showed that lactic acid was the major organic acid detected, followed by succinic acid and acetic acid. The addition of tartaric and/or garcinia acids was proven to reduce the fermentation time. Therefore, the addition of acid has assisted the fermentation process by increasing the total organic acid content, mainly lactic acid. Moreover, the 9-point hedonic scale showed that ikan pekasam made of Javanese carp had higher overall acceptability due to tender flesh/muscle texture compared to black tilapia regardless of the fermentation process and organic acid content

BLACK TILAPIA FISH (Oreochromis niloticus) SKIN EXTRACT INDUCE WOUND HEALING ON Rattus norvegicus

Objective: The objective of this research is to evaluate the effect of black tilapia sh/BTF (Oreochromis niloticus) skin extract cream application on wound healing by assessing its effect on wound contraction, epithelial thickness, and collagenization rate. T Method: rue experiment using 36 Rattus norvegicus as research object, wounded with punch biopsy sized 8 mm on right side of the back. Research objects were divided into 6 groups (X0: no treatment; X1: cream base application on wound; X2: application of gentamicin cream; X3: application of BTF extract cream 10%; X4: application of BTF extract cream 20%; X5: application of BTF extract cream 30%. Wound contraction was evaluated on day 3, 6, 9, and 11. Tissue sample was biopsied and examined on day 11 to evaluate epithelial thickness (HE) and collagenization (mallory). Application of Result: BTF skin extract cream showed an effect on wound contraction, epithelial thickness, and collagenization rate of new wound, the higher the concentration of BTF cream given, the better the result was. BTF skin extrac Conclusion: t cream can be a new hope and alternative therapy in wound treatment.

Utility of DNA barcoding in native Oreochromis species.

The importance of Oreochromis in worldwide aquaculture and regional fisheries motivates the study of their genetic diversity in their native range. In this article, all mitochondrial cytochrome c oxidase subunit I gene (COI) sequences of Oreochromis species are retrieved from Barcode of Life Data system to quantify the available DNA barcoding information from wild individuals collected within the native ranges of the respective species. It is found that 70% of the known species in the genus still lack a COI barcode, and only 15% of the available sequences are from within the respective native ranges. Many of the available sequences have been produced from specimens acquired from aquaculture and introduced, naturalized populations, making the assessment of variation within the original native range challenging. Analyses of the wild-collected fraction of available sequences indicated the presence of cryptic lineages within Nile tilapia Oreochromis niloticus and O. schwebischi, the occurrence of potential introgressive hybridization between O. niloticus and blue tilapia O. aureus, and potential ancestral polymorphism between Karonga tilapia O. karongae and black tilapia O. placidus. This article also reports a case of misidentification of O. mweruensis as longfin tilapia O. macrochir. These results stress the importance of improving the knowledge of genetic variation within the native ranges of Oreochromis species for better-informed conservation of these natural resources.

Effects of Ultraviolet (UV) Treatment on the Properties of Black Tilapia Fish Skins Gelatin

The aim of the study is to investigate the effects of ultraviolet (UV) treatment on the properties of black tilapia fish skins gelatin. The fish gelatin were investigated in terms of gel strength, functional group and the water contact angle of the gelatin. The UV treatment were irradiated with UVA and UVC at different time (0.5, 1.0, 1.5 and 2.0 h). The gel strength of gelatin gel significantly increases after UV irradiation for both UVA and UVC sample. The water contact angle of the gelatin was categorized as hydrophobic for both gelatin that treated with UVA and UVC which the angle >65°. The interactions and characteristic of functional groups for gelatin that treated with UV were analyzed via Fourier Transform Infrared Spectroscopy (FTIR). Results indicated that employing UV irradiation as an alternative method to enhance some of the quality attributes of fish gelatin.

Determination of lead and cadmium in tilapia fish (Oreochromis niloticus) from selected areas in Kuala Lumpur

Bioaccumulation of heavy metals in aquatic animals causes serious threat to human health via persistent consumption of fish. This study was conducted to determine the concentrations of lead (Pb) and cadmium (Cd) in various organs of Oreochromis niloticus (black tilapia) and its potential human health risk. The fish were collected from Sri Murni Lake and Kepong Metropolitan Lake around Kepong district, Kuala Lumpur. Pb and Cd concentrations in fish organs were measured using dry ashing-acid digestion method and analysed by atomic absorption spectrophotometer (AAS). The highest concentrations of Pb were detected in the gills (0.151 ± 0.12 mg/g) followed by bones (0.108 ± 0.09 mg/g) and the least in muscle tissues (0.078 ± 0.05 mg/g). Cd was highly accumulated in the bones (1.750 ± 3.43 mg/g) compared to gills (0.083 ± 0.05 mg/g) and muscle tissues (0.004 ± 0.01 mg/g). Pb and Cd concentrations in fish muscles collected from both sites were within the maximum allowable limits according to Malaysian Food Regulations 1985. In the assessment of potential health risk, the hazard quotient (HQ) for Pb and Cd was found to be <1 to indicate that the consumption of tilapia fish muscles obtained from the defined areas is hazardless and safe for human.

Effect of Thermal Treatment on the Physicochemical Properties of Emulsion Stabilized by Gelatin from Black Tilapia (Oreochromis mossambicus) Skin

This study investigated the influence of thermal treatment (30 °C to 110 °C, 30 min) on the physicochemical and rheological properties of an emulsion stabilized by black tilapia (Oreochromis mossambicus) skin at pH 4. The protein pattern of tilapia gelatin did not have any significant difference after the gelatin was heated within a temperature range of 30 °C to 70 °C. However, at 90 °C and 110 °C, denaturation occurred where α-, β- and γ-chains of the gelatin were degraded, leading to a concomitant increase in low molecular peptides. The emulsion stability was investigated through a particle size analyzer, zeta potential, microscopic observation and creaming index. The gelatin emulsion was physically stable at 30 °C to 70 °C with a mean droplet size of less than 13 μm. When the heating temperature was increased to 90 °C and 110 °C, the emulsion showed a pronounced increase in droplet size due to coalescence. The gelatin emulsion heated at 90 °C and 110 °C also displayed instability against creaming after storage at room temperature for 7 days. As the heating temperature increased, the gelatin emulsion exhibited a decrease in apparent viscosity and the flow behavior changed from shear thinning to Newtonian. The rheological data also showed that the storage modulus (G′) of emulsion became more frequency dependent as the heating temperature increased, indicating weak droplet interactions. The tilapia gelatin emulsion was physically unstable when subjected to thermal treatment above 70 °C. The data reported in this study provides useful insight into the formulation of acidic food emulsions that require thermal treatment.

EVALUASI PERFORMA PERTUMBUHAN DAN HETEROSIS PERSILANGAN ANTARA IKAN NILA NIRWANA (OREOCHROMIS NILOTICUS) BETINA DENGAN IKAN NILA BIRU (OREOCHROMIS AUREUS) JANTAN F2 PADA KONDISI TAMBAK HIPERSALINITAS

Srikandi Tilapia is one of the superior hybridized varieties between black tilapia (Oreochromis niloticus) and blue tilapia (Oreochromis aureus). This fish has fast growth and high salinity tolerance. In its development, efforts are needed to improve and improve its genetic quality. Spawning srikandi tilapia using the parent of male blue tilapia F2 (family selection) is expected to improve performance better than the previous generation.The purpose of this study was to evaluate the growth performance and heterosis value of Srikandi tilapia produced from the extraction of female Nirwana strain tilapia with male blue tilapia F2. Hatchery activities were carried out in freshwater by mass spawning method on the 25 m2 pool. Enlargement activities were carried out at brackish water pond with salinity 25–40 g / L, using net measuring 3×5×1 m3 for 120 days. The stocking density used is 15 fishes / m2. The results of this study indicate that at the final test, the hybridization of Nw ♀x Au (F2)♂ has the best performance with the average of long gain16.60 ± 0.41cm, weight gain 215.33 ± 12.19 g and survival rate 78.50 ± 02.12. Evaluation of heterosis values on the characters of length, weight, survival, and biomass showed a positive value both on the hybridization of Nw ♀x Au (F2)♂ and Au (F2)♀x NW♂. The highest high parent heterosis value for blue tilapia broodstock is shown by population Nw ♀x Au (F2)♂ with values in each character of 6.96% (length), 26.06% (weight), 2.63% (survival), 28.57% (biomass).