Sample Preparation of the Natural Source Hydroxyapatite (HAp) derived from Black Tilapia Fish Scales

Abstract

Hydroxyapatite (HAp) is a biomaterial with the chemical formula Ca10(PO4)6(OH)2. Normally, it was used and applied in biomedical applications, cooking recipes and healthy food products. Due to this, it has attracted the researcher to work on HAp synthesis and extraction. In this study, the hydroxyapatite was extracted from fresh water Black Tilapia Fish Scales (BTFS) was analysed. The crystallinity of the HAp was characterized by using an X-ray diffractometer (XRD) whereas the Fourier transform infrared (FTIR) analysis was used to characterize the presence of HAp from the samples. Scanning electron microscopy (SEM) provided with an Energy Dispersive Spectroscopy, (EDS) was used to investigate the morphology and element of the hydroxyapatite powders. The BTFS samples were heated at 100 °C to composed and eliminated the remaining meat and other impurities. A mechanical crusher was applied to make a miniscule and fine hydroxyapatite powder using a thermal calcination technique using a furnace. The BTFS samples powder were calcined at a temperature of 900 °C for 3 hours correspondingly. The XRD spectra findings, uncovered the existence of the obtained BTFS HAp are articulate with Joint Committee on Powder Diffraction Powder Standard data (JCPDS) from the library. From the SEM results, HAp powder presence in hexagonal shape. The findings show the potential of producing high-value products from fishing waste, such as HAp with Ca/P ratio 1.74, for biomedical applications.