This paper is the second part of a two-paper series on framework development and application demonstration of the Inclusive Impact Index (Triple I), a single-index of sustainability assessment. This Part II aims to exemplify findings obtained from Part I, which developed a full-scale framework for estimation of Triple I, based on the current context of life cycle sustainability assessment. The entire life cycle of inedible vegetable oil-derived biodiesel production and use in cruise ships in Ha Long Bay, was used as a case study for the demonstration. Furthermore, this paper also examines the sustainable potential of several blends of Hibiscus sabdariffa L. (Roselle) and Vernicia montana L. (Trau) biodiesel. Consequently, implications for sustainable biodiesel policies in Quang Ninh Province have been introduced.Under Triple I, the sustainable potential of different biodiesel systems in Ha Long Bay was examined with respect to impacts on ecological footprint, biocapacity, ecosystem quality, human health and the economy. Several biodiesel-petroleum diesel blends were considered in this paper involving B5 (5% by volume (vol%) biodiesel), B10 (10 vol% biodiesel), B20 (20 vol% biodiesel) and B100 (neat biodiesel). Triple I gages the sustainability of all biodiesel blends as alternatives to petroleum diesel. The findings suggest that the application of B10 and B20 blends are most appropriate, as they were the most stable and equivalent in terms of economic, environmental and social impacts, particularly in the case of B20. Since B100 has considerably high sustainable potential but longer Triple I payback time compared to the three lower blends, this study proposes a consideration of B20 and B10 for short-term and B100 for long-term biodiesel application pathways.Results of Triple I are in line with previous studies, and this confirms the precision of Triple I as a sustainability assessment index. On the other hand, by quantitatively revealing what and how an individual factor and its changes can affect the sustainability of a biodiesel system, Triple I has proved its superiority to other indexes. Nevertheless, due to the absence of social impacts in the Triple I estimation, this study recommended for a further development of the Triple I framework to contemplate evaluating social aspects.