Bark Powder Research Articles

Use of a hydroalcoholic extract of Salix alba L. bark powder in diets of broilers exposed to high heat stress

A study was conducted to determine the effects of dietary hydroalcoholic willow bark extract powder (HWE) supplemented to broilers (14–42 days old) that were exposed to heat stress, on the performance, serum biochemical parameters, liver oxidative status and caecal microflora. The feeding trial was conducted on 120 Cobb 500 broilers (14 days old), assigned to three treatments (T0, T25, and T50), each treatment consisting of eight replicates (five chicks per replicate). The broilers were housed in an experimental hall at a 32 °C constant temperature and 23 hours light regimen. Unlike the dietary control treatment (T0), the experimental treatments were supplemented with 25 g HWE powder/100 kg diet (T25), and 50 g HWE powder 100 kg diet (T50), respectively. Dietary HWE powder did not affect the broilers’ performance significantly (14–42 days). A significantly lower amount of malondialdehyde was noticed in the liver of broilers from T25 and T50 treatments in comparison with broilers from T0. Also, the serum cholesterol, triglycerides and alanine aminotransferase were significantly lower in broilers fed with T50, compared with those fed with T0. At 35 and at 42 days, the broilers from T25 and T50 recorded a significantly lower number of E. coli and staphylococci and a higher number of lactobacilli in the caecum than those of T0. It could be concluded that supplementation of dietary HWE powder reduced some of the adverse effects of heat stress, the most effective being the level of 50 g/100 kg diet.Keywords: biochemical parameters, caecal microflora, high temperature, liver oxidative status

The Use of Walnut Tree Bark Powder as a Teeth Whitener

The chemical changes that may occur with carious destruction of enamel lead to unacceptable appearance of teeth. For males and females the appearance of teeth is of most importance, therefore they should take care with their teeth. The cleaning and whitening processes are expensive and may be harmful for enamel of teeth, so this research includes the whitening of teeth by using natural product containing toothpaste. The powdered bark of walnut tree has been used as whitening material in four forms and these are tested on 40 volunteers with stained teeth in dentist clinic. The four products were powder only. Powder mixed with toothpaste, condensed aqueous extract and granules of the product. The percentage of the powder in the paste was 5%, the mixture is homogenized by using mortar and pistil and the pH is tested and calibrated by pH meter to 6.5 which is the acceptable medium for mouth and is included the normal range of saliva pH. The product is examined by brushing gently for two minutes after the brushing for same period by toothpaste without the powder. Walnut bark contains high quantities of minerals particularly manganese, and contains important subgroup of polyphenolic compound called ellagitannins which have antibacterial effects and antioxidant properties. The differences before and after brushing with powder were highly significant.

CYTOTOXICITY STUDIES OF AN ETHNOBOTANICALLY SELECTED SOLANUM PUBESCENS WILLD. AGAINST SELECTED HUMAN CANCER CELL LINES

Objective: The present study was aimed to determine the cytotoxicity concentration (CTC50) of different extracts made from the leaf and stem bark of an ethno botanically selected S. pubescens against Human liver carcinoma (Hep G2), Human colon carcinoma (CaCo2) and Human breast cancer (T-47 D) cell lines.
 Methods: Ethnobotanical survey was done through interviewing traditional medicinal practitioners then a potential herbal plant was selected after a thorough literature survey and its identity was confirmed. The soxhlet extraction method was adopted using five different solvents from leaf and stem bark powders of the study plant and the CTC50 of all the extracts were determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay method.
 Results: Among the different extracts, CTC50 values were significant for stem bark extracts made from methanol (24.51±0.08 µg/ml) against Hep G2, while leaf chloroform extract was promising (57.15±1.75 µg/ml) against CaCo2 and n-hexane extract of leaf exhibited significant value (20.27±1.52 µg/ml) against T-47 D cancer cell lines.
 Conclusion: The major findings of the present study clearly provides evidence that the leaf and stem bark of S. pubescens possesses the potential anticancer bioactive compound solasodine.

Investigation of Walnut Herbal Drug Raw Material

Introduction. Walnut (Juglans regia L.) is a medicinal plant containing a large number of biologically active substances. A number of authors propose to use walnut leaves as medicinal raw materials, in which juglone, flavonoids, tannins, ascorbic acid and other substances are identified. Currently, a standardization technique for this type of raw material has been developed and proposed. However, the medicinal plant walnut can be a source of other types of raw materials, such as milky-waxed walnuts, walnut bark, and walnut partitions. These types of medicinal raw materials contain a large number of biologically active substances and have a great potential for pharmacological activity. Therefore, the study with the subsequent standardization of medicinal plant material of walnut fruits in the stage of milk-wax ripeness and walnut bark is relevant.Aim. The aim of the study is to study the external signs, microscopy of raw walnut bark and walnut fruit in the stage of milky-wax ripeness and chemical composition of alcohol extract from these types of raw materials.Materials and methods. Chromato-mass spectrometry and TLC were used in the work.Results and discussion. When describing external signs and microscopy, diagnostic signs of both whole and crushed raw materials, and powder of walnut bark, and fruits of walnut in the stage of milky-wax ripeness were revealed. Alcohol extract obtained from medicinal plant raw materials walnut bark is a clear brown liquid. Alcohol extract obtained from medicinal plant raw materials of walnut fruit at the stage of milky-wax ripeness is a transparent, green-brown liquid with a fragrant odor.Conclusion. Gallic acid was identified by TLC in walnut bark. Chromato-mass spectrometry in alcohol extraction from the fruit of walnut in the stage of milky-wax maturity was able to identify 17 compounds belonging to different classes of biologically active substances. Sugars, flavonoids, coumarin phenolic compounds, organic acids were identified. In alcoholic extraction from the bark of a walnut, marker compounds have been determined, the combination of which makes it possible to quickly identify this type of raw material using chromatography-mass spectrometry.

Bonding strength of plywood bonded using phenol formaldehyde mixed with wood bark powder nanofiller

This paper investigates the wettability of selected wood species from Indonesian community forests, namely (Indonesian name–Latin name) surian (Toona sinensis), jabon (Anthocephalus cadamba), gmelina (Gmelina arborea.), Manglid (Manglietia glauca), rubber wood (Hevea brasiliensis), and sengon (Paraserianthes falcataria). The study measures the contact angle and evaluates the suitability of these wood species for plywood production. Logs from a community forest were processed to convert into thin slices of veneer using the rotary cutting method at a plywood plant. Subsequently, the study analyzed the wetting behavior of the veneer samples resulting from a rotary process on tangential surfaces without smoothing treatment with distilled water. The veneer was then made into plywood using an adhesive nanofiller of phenol formaldehyde mixed with wood bark powder. Ball milling produced nanofillers from the bark of jabon, gmelina, and surian with nanoscale particle diameter size (10–1000 nm). The bonding strength of the plywood was evaluated under cyclic and dry test conditions according to Japanese Standards. The study shows that P. falcataria, H. brasiliensis, and A. cadamba have a smaller contact angle than T. sinensis, G. arborea, and M. glauca. Thus, the higher wettability of P. falcataria, H. brasiliensis, and A. cadamba results in a better adhesive spread and more intimate contact between the wood surface and the adhesive. However, the study found higher bonding strength values for H. brasiliensis and T. sinensis plywood using jabon nanofiller, followed by T. sinensis plywood using surian nanofiller and H. brasiliensis plywood using gmelina nanofiller.

Cinnamon powder: an in vitro and in vivo evaluation of antifungal and plant growth promoting activity

In this study, the effects of organic powder of Cinnamomum zeylanicum on the development of Botrytis cinerea and its influence on tomato plants were evaluated. The cinnamon bark powder and its water suspensions and filtrates were used at 0.5 and 1% rates. After 6 days of the start of an in vitro experiment the mycelium growth was inhibited by both 0.5 and 1% cinnamon water filtrates - to a greater degree in the case of the higher concentration, by 54.4 and 81.4%, respectively. Spraying with cinnamon water filtrates positively influenced the growth of plants both in the greenhouse and the field. Antifungal activity of cinnamon was proved in the greenhouse tests - the disease symptoms of grey mould on infected tomato plants decreased. The fresh weight of non-inoculated tomato plants treated with cinnamon filtrates was significantly higher than control plants (17.17 g compared to 12.83 g) showing a stimulating effect of cinnamon filtrates. In the case of inoculated plants due to treatment, their weight increased from 7.83 to 10.50 g. In the field experiment, tomato plants sprayed six times with cinnamon were better developed than the control plants. The most significant effect was observed for Hamlet variety - the mean number of leaves was higher by 27.3% and the mean number of branches by 19.7% compared to the untreated control plants. Thus it was proved that cinnamon powder has potential to inhibit B. cinerea growth and also has a stimulating effect for tomato plants.

TO STUDY THE ANTIBACTERIAL ACTIVITY OF VARIOUS EXTRACTS OF CLAUSENA DENTATA (WILLD.) ROEM.

Objectives: The objectives of the study were to study the antibacterial activity for the various extracts of Clausena dentata against human pathogens. Clausena (Rutaceae) is a genus of about 23 species of unarmed trees and shrubs. The stem bark of C. dentata is used in veterinary medicine for the treatment of wounds and sprains. Even though C. dentata has a lot of potential medical uses, the study of microbiological properties is very scarce.
 Methods: The plant C. dentata was collected from Kadagaman, near Tiruvannamalai, Tamil Nadu, India, and authenticated by Centre for Advanced Study in Botany, University of Madras, Chennai. The dry powder of stem bark was extracted with hexane, chloroform, and methanol. The extracts were subjected to qualitative phytochemical screening and antibacterial activity against human pathogenic bacteria such as Escherichia coli, Salmonella Typhi, Klebsiella pneumonia, Vibrio cholerae, and Staphylococcus aureus and compared with ciprofloxacin.
 Results: Qualitative chemical tests revealed the presence of various phytochemicals such as alkaloids, glycosides, carbohydrate, proteins and amino acids, phytosterols, and volatile oil. The antibacterial activity result reveals that all the extracts were are more active against V. cholerae. The activity against Pseudomonas aeruginosa was mild.
 Conclusion: The activity against V. cholerae was comparable with that of 5 μg/mL ciprofloxacin at the concentration of C. dentata 40 μg/mL. The orders of antibacterial activity against human pathogenic bacteria are hexane, methanol, and chloroform extract of C. dentata.

Exploration of Tensile, Flexural and Hardness Test Properties of Prosopis Juliflora / Glass / Epoxy Hybrid Composite Laminates

This work aims on utilising the potential benefits of Prosopis Juliflora which is abundant and is a threat to environment. Many works were done on prosopis Juliflora wood to bring out the thermal properties and this is the first time we tried to bring out the mechanical properties of its bark powder as in the form of composites. This work extracts the mechanical benefits of Prosopis Juliflora powder, glass fibre mat and epoxy resin to be suitable for the structural applications. Specimen with above hybrid composites were made and major tests were done to prove it to be suitable for structural applications. Experimental values were to br in good agreement with the theoretical.

TO STUDY THE IN VITRO INHIBITION OF BLOOD PLATELET AGGREGATION BY ISOLATED COMPOUNDS FROM CLAUSENA DENTATA (WILLD.) ROEM.

Objectives: The objectives of the study were to study the antiplatelet aggregation activity of compounds isolated from extracts of Clausena dentata. Clausena (Rutaceae) is a genus of about 23 species of unarmed trees and shrubs. The stem bark of C. dentata is used in veterinary medicine for the treatment of wounds and sprains. Even though C. dentata has a lot of potential medical uses, the study of pharmacological properties is very scarce.
 Methods: The plant C. dentata was collected from Kadagaman, near Tiruvannamalai, Tamil Nadu, India, and authenticated by Centre for Advanced Study in Botany, University of Madras, Chennai. The dry powder of stem bark was extracted with hexane, chloroform, and methanol. The extracts were subjected to qualitative phytochemical screening and column chromatography. Four compounds were isolated. All the isolated compounds were subjected to adenosine diphosphate (ADP)-induced platelet aggregation and compared with aspirin.
 Results: The isolated compounds from C. dentata and standard aspirin showed significant antiplatelet activity against ADP-induced platelet aggregation.
 Conclusion: The compounds, 3-(1,1-dimethylallyl)xanthyletin, dentatin, nordentatin, and carbazole showed significant antiplatelet aggregation activity. Among the compounds, nordentatin showed more activity of antiplatelet aggregation.

Development of a standardized combined plant extract containing nutraceutical formulation ameliorating metabolic syndrome components

Plant bioactives have a great role in combating multifactorial disease conditions like metabolic syndrome (MetS). This research work aims to develop a standardized plant extract combination and formulate it to tablets with nutraceutical potentials. The extract was prepared from the bark powder of Ficus religiosa, seed powder of Syzigium cumini and leaf powder of Ocimum bacilicum chemometrically optimized in the ratio of 1.15:1.15:1.68 (Ashwattha: Jamun: Basil or FR: SC: OB). It is enriched in screened and pharmacologically active plant secondary metabolites. The tablets were prepared by direct compression using single-punch tablet machines. The nutraceutical tablets passed all the prescribed quality control tests with a justified pharmacokinetic profile. Results of animal experimentations have shown the hypoglycemic, hypolipidemic effect and antihypertensive effect of the nutraceutical tablets in relevant animal models. Thus, the nutraceutical formulation that showed effectivity in combating MetS can be opted as an adjunct therapy.

Evaluation of Fibrolytic Effect of Bauhinia purpurea L.

Background: Mammary gland tissue (left half) revealed the presence of excess proliferation of fibrous tissue with disorganization of alveolar structures and the right half showed extensive fibrous tissue proliferation of mammary gland following intramammary inoculation of 2000 c.f.u. of Staphylococcus aureus. However, oral dosing of Bauhinia variegata L. bark powder at 6 gm/kg for 7 days and 3 gm/kg for another 7 days exhibited reduction of fibrous tissue in chronic mastitis. Objective: The fibrolytic effect of one week oral dosing of Bauhinia purpurea L. bark powder was studied in chronic mastitis with induced fibrosis as Bauhinia variegate L. is rarely available in plain land. Methods: Chronic mastitis with fibrosis was induced by intramammary inoculation of coagulase positive Staphylococcus aureus in group III and IV goats. Group I and III goats were injected with a single dose of ceftriaxone at 20 mg/kg intravenously, whereas group II and IV goats were orally administered Bauhinia purpurea L. bark powder at 6 g/kg for 7 days with a single dose of ceftriaxone at 20 mg/kg intravenously. Results: The t1/2β of ceftriaxone with Bauhinia purpurea L. stem bark powder in chronic mastitis increased significantly. Ceftizoxime was detected in plasma from 1 h to 48 h post dosing (pd) in group III and from 1 h to 96 h pd in group IV, respectively. Conclusion: Bark powder of Bauhinia purpurea L. at 6g/kg orally once daily increased the bioavailability of ceftriaxone and or ceftizoxime in milk due to its fibrolytic effect which was not reported earlier. Therefore, the Bauhinia purpurea L. bark powder having fibrolytic effect has the potential to reduce development of cancer.

Quality of Leather Using Vegetable Tannins Extract of Acacia Mangium Bark from Waste of Industrial Plantation

The purpose of this study was to determine the characteristics of leather using vegetable tanning ingredients from the bark of Acacia mangium Willd. Tanning agent ingredients are provided in the form of bark powder and extract. The first phase of the preparation of the bark into powder and the second stage is the extraction of tannin from A.mangium bark. Extraction using water at 1000C for 30 minutes then evaporated so that tannin extract was obtained. Then the second stage is carried out the tanning process using both materials. Data analysis uses paired two sample for means T-test. The results of statistical analysis showed significant differences in the characteristics of the leather produced. Chemical analysis of leather tanned using A.mangium bark obtained moisture content of 16.76%, ash of 3.42%, tannin bound 18.99% and degree of tanning 38.21% while chemical analysis of leather tanned using tanneries A.mangium bark extract which is moisture content 16.03%, ash 4.18%, bound tannin 32.41% and 84.82% tanning degree. The physical properties of leather using A.mangium bark powder were tensile strength 493.84 kg / cm2, elongation 34.94%, zwik strength / lastability 6.66 mm (leather cracked). Compared to the characteristics of leather using tanner A.mangium bark extract, tensile strength was 254.32 kg / cm2, elongation 44.39%, zwik / lastability strength 8.63 mm (leather not cracked).

Resinless Plywood Production by Using Oxidized Acacia Bark Powder as a Binder

Nowadays, the established technology in plywood industries for producing plywood is still using adhesive as veneer bonding agent. Utilization of the adhesive has several of weaknesses such as containing formaldehyde compound and using the unrenewable raw material. The purpose of this research was to develop a novel technique to produce plywood without involving adhesive as a binder. The oxidized bark powder of Acacia mangium was utilized as a binder to substitute the adhesive. Bark powder was oxidized by using hydrogen peroxide on four levels (5%, 10%, 15%, 20%) and the catalyst. The result showed that utilization of oxidized bark powder can be applied in producing resinless plywood. Unless the plywood with 15% of hydrogen peroxide, the other plywood fulfil the National Indonesian Standard (SNI) No 01-5008.2-2000 where min. 7 kg/cm2 as the requirement. The plywood with 20 percent of hydrogen peroxide has the highest bonding strength (13.24 kg/cm2). After immersing this plywood in hot water (70°C) for 2 hours and drying in the oven (60°C) for 3 hours, the wood failure test also showed a good value, namely 76.15 percent. These facts indicated that the bonding and the water resistant of the plywood were relatively high. It tends that the technology for producing resinless plywood is potential to be developed.

Antibacterial Effect of Cinnamon (Cinnamomum zeylanicum) Bark Extract on Different Bacterial Isolates

In this study, the antibacterial activity of Cinnamon (Cinnamomum zeylanicum) bark ethanolic extract was investigated on different bacterial isolates (Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa) of clinical origin. The ethanolic extract of Cinnamon was extracted from the Cinnamon bark (spice) powder using ethanol, and various standard concentrations of the Cinnamon extract were aseptically impregnated onto sterile circular discs. Pure culture of the bacterial isolates was subjected to antimicrobial susceptibility test using the Agar Disc Diffusion method. The findings of this study revealed E. coli produced the highest susceptibility to the cinnamon extract; S. aureus was intermediately susceptible while P. aeruginosa was least susceptible to the highest concentration of the cinnamon extract. Reasonably, the lowest concentration (20 % (v/v)) of Cinnamon extract also had minimal antibacterial action only on S. aureus but E. coli and P. aeruginosa exhibited resistance to this concentration of Cinnamon bark ethanolic extract. This study portrayed Cinnamon as an antibacterial agent and serves as a pointer for pharmaceutical industries in producing effective antibacterial drugs of plant sources.

Biospecific separation of holmium(III) using raw and chemically treated bark powder of Mangifera indica: kinetics, isotherm and thermodynamic studies

ABSTRACT In this article, the biosorption of Holmium(III) from aqueous solution was carried out using both raw and acid-treated bark powder of Mangifera indica as bio-sorbents and the results are being compared. Batch experiments were conducted by varying different reaction parameters such as metal concentration, biomass dosage, pH of the metal solution and contact time. The results showed that the acid-treated biomass exhibited higher percentage removal of the metal ion Ho(III) i.e. 93.44% than the untreated bark powder using two times lesser amount of treated bark powder than the untreated one. Kinetics, isotherm and thermodynamic studies of adsorption were carried out. Kinetics of the adsorption process follows the linear form of a pseudo-second-order model. The negative values of Gibbs free energy change (ΔG ◦) indicate the spontaneity of the adsorption process and the positive value of enthalpy change (ΔH ◦) suggests it as an endothermic process. Out of four isotherm models, the equilibrium data were well described by Langmuir adsorption isotherm conforming monolayer coverage. The results suggested that acid-treated bark powder of M. indica can be used as a cost-effective and efficient bio-adsorbent for the adsorption of Holmium(III).

Pharmacological study of trunk bark of Acacia nilotica var adansonii (Guill et Perr).o Ktze (Mimosaceae): Assays, antioxidant and antispasmodic activities

Aim of this study was to evaluate in vitro polyphenols content, antioxidant and antispasmodic properties of the aqueous extract and fractions of the trunk bark of Acacia nilotica. According to a survey conducted in rural Burkina Faso, Acacia nilotica var. adansonii (Guill and Perr). Ktze reported to be widely used in the treatment of gastrointestinal diarrhoea and parasitosis. A maceration of the powder of the trunk bark of the plant was carried out. Then the aqueous macerate obtain, was fractionated with dichloromethane, butanol and ethyl acetate successively. The phenolic compounds of the aqueous extract, butanol and ethyl acetate fractions was determinated. The antioxidant activity of aqueous extract and fractions was evaluated by the DPPH, ABTS and FRAP tests. The contractility test on smooth muscle was realized according to Magnus method. Assay of the extracts revealed a high content of polyphenols, tannins and flavonoids. The aqueous extract, the butanol fraction and the ethyl acetate fraction demonstrated a high antioxidant capacity. Aqueous extract showed a better antispasmodic effect of acetylcholine contraction induction at 1 μM (IC50 = 13.02 μg / mL) and for BaCl2 at 160 μg / mL (IC50 = 117.2 μg / mL). The aqueous extract of Acacia nilotica and his fractions had antioxidant properties. Only aqueous extract proven better antispasmodic property. Hence its use in traditional medicine in the treatment of diarrhoea. Keywords: Acacia nilotica, Antioxidant, Antispasmodic

Toxicological and Histopathological Effects of Cleistopholis patens Benth Root Bark Powder Used as Cowpea Protectant and Medicine

Toxicological and Histopathological Effects of Cleistopholis patens Benth Root Bark Powder Used as Cowpea Protectant and Medicine

Chemical composition and antioxidant properties of some industrial tree bark extracts

Wood bark is a residue of forestry production that is used as a fuel source. The chemical composition of tree bark is similar to that of the harvested wood, and it contains a variety of useful compounds. To determine the chemical composition and antioxidant activities of different barks, fir (Abies nordmanniana), beech (Fagus orientalis), pine (Pinus sylvestris), poplar (Populus alba), and oak (Quercus robur) barks were selected because they are used for industrial purposes in Turkey. The dried bark powders were extracted using a 65:35 methanol-water mixture (v/v) to determine the total phenolic content, the flavonoid content, and the antioxidant properties (2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), metal chelating, and H2O2 scavenging). The flavonoid components were analyzed by high performance liquid chromatography (HPLC) and extracted by hexane to analyze the volatile components by gas chromatography-mass spectrometry (GC-MS). The poplar bark extracts had the highest total phenolic content, highest total flavonoid content, and highest antioxidant content. The poplar bark extracts were rich in myricetin (87.761 mg/L), which is a flavonoid with rich antioxidant properties. The presence of valuable extracts suggests that barks may have uses as valuable raw materials for chemical applications such as cosmetics, perfumes, and food preservatives.

Anticancer activity of Gnidia glauca bark extracts against MCF-7 cell lines and isolation of a lignan as the marker compound

The search for a complementary and alternative medicine has gained attention in recent years due to pronounced side effects and hazards of synthesized drugs. Hence, the exploration of novel bioactive components from traditionally used medicinal plants is necessary. Gnidia glauca is a semi-woody herb of the Thymelaeaceae family and is traditionally used as fish poison in India. Dried bark powder of G. glauca was subjected to physicochemical evaluation and then extracted with n-hexane, chloroform, ethyl acetate, ethanol, and water by successive Soxhlet extraction. All the extracts were tested for anticancer activity on human breast cancer cells (MCF-7 cell line) using sulforhodamine B colorimetric assay. Amongst all, ethanolic and ethyl acetate extracts were found to have significant cytotoxic activity. Further ethanolic extract was subjected to flash chromatographic separation for the isolation of a suitable marker, followed by its quantification by HPTLC. After identification, the compound was studied by infrared (IR) spectroscopy and high-resolution mass spectrometry to get insights into its structure.

Application of cinnamon and gotu kola supplements for increasing quail hematological status (Coturnixcoturnix-australica)

Cinnamon and gotu kola are herbal plants that are rich in polyphenol-type antioxidants. This type of antioxidant has been known to have an important role in stimulating an increase in biological function, especially hematological status. Quail with increased haematological status can be known from hemoglobin levels and erythrocyte counts per mm3 of blood. The aim of this study was to analyze the hematological status of quail Australian strains by adding cinnamon and gotu kola leaves supplements with variable hemoglobin levels, erythrocyte levels, and body weight supporting variables. The study design used a Completely Randomized Design consisting of 8 treatments with 3 replications. The treatment given includes control, feed supplemented with cinnamon bark powder 5% or 10%, supplementation of gotu kola leaf flour 5% or 10%, combination of cinnamon powder flour supplement with gotu kola (5%: 5%, 5%: 10 % or 10%: 5%). The results of the study were analyzed by Analysis of Variance (ANOVA) which continued with Duncan’s Test at a significance level of 5%. The results showed that the supplement of cinnamon bark and gotu kola leaves in feed gave a significant effect on body weight, hemoglobin levels, and the number of erythrocytes (P <0.05). A combination of gotu kola cinnamon-bark supplement with a ratio of 5%: 10% gives an effect on increasing hemoglobin and erythrocyte levels with a higher value than other treatments, which is equal to 13 g / % and 2,588,000 per mm3. Quail body weight in each treatment was not significantly different. The conclusion of this study is the addition of combination combinations of gotu kola cinnamon flour with a ratio of 5%: 10% can improve the hematological status of quails, so that the composition of this combination of ingredients can be used to increase the productivity of Australian strains quail laying.