Abstract
Objective: The present study was aimed to determine the cytotoxicity concentration (CTC50) of different extracts made from the leaf and stem bark of an ethno botanically selected S. pubescens against Human liver carcinoma (Hep G2), Human colon carcinoma (CaCo2) and Human breast cancer (T-47 D) cell lines.
 Methods: Ethnobotanical survey was done through interviewing traditional medicinal practitioners then a potential herbal plant was selected after a thorough literature survey and its identity was confirmed. The soxhlet extraction method was adopted using five different solvents from leaf and stem bark powders of the study plant and the CTC50 of all the extracts were determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay method.
 Results: Among the different extracts, CTC50 values were significant for stem bark extracts made from methanol (24.51±0.08 µg/ml) against Hep G2, while leaf chloroform extract was promising (57.15±1.75 µg/ml) against CaCo2 and n-hexane extract of leaf exhibited significant value (20.27±1.52 µg/ml) against T-47 D cancer cell lines.
 Conclusion: The major findings of the present study clearly provides evidence that the leaf and stem bark of S. pubescens possesses the potential anticancer bioactive compound solasodine.
Highlights
Cancer is all concerning uncontrolled cell growth [1], it is escalating at an alarming rate causing a threat to human life and sustainability
Selection of S. pubescens was accomplished through an ethnobotanical survey, a herbal medicinal plant used by the traditional medicinal practitioners to treat cancer
A sum of 190 plant species belonging to 156 genera and 75 families were screened from six taluks of Chitradurga district whereas in the literature cytotoxicity study of S. pubescens is not yet reported
Summary
Cancer is all concerning uncontrolled cell growth [1], it is escalating at an alarming rate causing a threat to human life and sustainability. Some of the villagers in the Chitradurga district used to approach traditional medicinal practitioners for various ailments and get treated. This indicates that documentation of the indigenous knowledge, conservation, domestication and better management of key medicinal plants is necessary to ensure intergenerational benefits from the herbal medicines [7]. Researches on the selection of medicinal plants for its phytochemical extraction and their cytotoxicity studies should begin with the ethnobotanical surveybased approach with traditional medical practitioner's knowledge. In the present study, S. pubescens, an ethnobotanically important plant was selected for the extraction of phytochemicals and investigation of their cytotoxicity against Hep G2, CaCo2 and T-47 D cell lines were made to identify a potent medicinal plant for anticancer drug molecules
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