ABSTRACT Objective: The purpose of this study was fivefold: to investigate the symptoms that would prompt collegiate aviation pilots perceive they are fatigued; to investigate the time of the day they are most fatigued; to investigate their academic and personal schedules; to investigate the methods collegiate aviation pilots utilize to ensure they are fit to fly; and to investigate whether they have received any academic and/or flight fatigue identification and management training. Background: Fatigue is a pervasive safety hazard in aviation affecting several aspects of flight crew members’ ability to perform their job. Fatigue in aviation and its consequences has been researched across military and commercial operations, but until now Part 141 collegiate aviation pilots have been neglected. Method: Data were collected using an online survey questionnaire self-report questionnaire (N = 122) consisting of items investigating fatigue identification and management by Part 141 collegiate aviation pilots. Results: Sixty percent of the participants usually experience the mental and physical symptoms of fatigue during flight activities. A finding of concern was that 43% of the participants indicated they had not received any training in fatigue identification and management during ground and flight activities. Conclusion: The safety management of fatigue in a Part 141 collegiate aviation environment is a safety issue that warrants further research, and training and education.