In continuous cover forestry, plenter silviculture is regarded as an elaborated system for optimizing the sustainable production of high-quality timber maintaining a constant but heterogeneous canopy. Its complexity necessitates high silvicultural expertise and a detailed assessment of forest stand structural variables. Terrestrial laser scanning (TLS) can offer reliable techniques for long-term tree mapping, volume calculation, and stand variables assessment in complex forest structures. We conducted surveys using both automated TLS and conventional manual methods (CMM) on two plots with contrasting silvicultural regimes within the Black Forest, Germany. Variations in automated tree detection and stand variables were greater between different TLS surveys than with CMM. TLS detected an average of 523 tree stems per hectare, while CMM counted 516. Approximately 9.6% of trees identified with TLS were commission errors, with 6.5% of CMM trees being omitted using TLS. Basal area per hectare was slightly higher in TLS (38.9 m3) than in CMM (38.2 m3). However, CMM recorded a greater standing volume (492.7 m3) than TLS (440.5 m3). The discrepancy in stand volume between methods was primarily due to TLS underestimating tree height, especially for taller trees. DBH bias was minor at 1 cm between methods. Repeated TLS inventories successfully matched an average of 424 tree positions per hectare. While TLS adequately characterizes complex plenter forest structures, we propose enhancing this methodology with personal laser scanning to optimize crown coverage and efficiency and direct volume measurements for increased accuracy of wood volume estimations. Additionally, utilizing 3D point cloud data-derived metrics, such as structural complexity indices, can further enhance plenter forest management.