Antioxidant Activity Of Ethanolic Extract Research Articles

CAPPARIS OVATA DESF. VAR. PALAESTINA ZOH. BİTKİSİNİN ÇİÇEK VE MEYVE EKSTRELERİNİN ENZİM İNHİBİTÖR ÖZELLİKLERİ VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI

In this study, the inhibiting effect on α-amylase and α-glucosidase and the antioxidant activity of ethanol extracts from flower and fruit of Capparis ovata var. palaestina was investigated. The antioxidant activity of extracts was tested with DPPH●, ABTS●+ and FRAP tests. According to the results, the highest α-glucosidase and α-amylase activity were found in fruit extract (IC50=271.72±0.41 µg/mL and 142.07±0.85 µg/mL, respectively). Also, the highest TPC (26.40±0.02mgGAE/gextract) and TFC (8.33±0.30 mgCA /gextract) was found in the same extract and this extract showed the highest DPPH● activity (%32). Although, flower extract showed the highest FRAP values (at a concentration of 1 mg/mL equivalent to 262.69±2.27 mmol Fe2+) and ABTS+● (at a concentration of 0.5 mg/mL equivalent to 0.18±0.01 µM Trolox) activity. In conclusion, it is thought that these studies will be shed light on future studies on this species.

Phytochemical profile, total phenolic content and antioxidant activity of ethanolic extract of fumitory (Fumaria capreolata L.) from Algeria

Phytochemical profile, total phenolic content and antioxidant activity of ethanolic extract of fumitory (Fumaria capreolata L.) from Algeria

EVALUATION OF INVITRO ANTI-OXIDANT ACTIVITY OF MAGNOLIA CHAMPACA

The aim of this article is to evaluate antioxidant activity of leaf extract of Magnolia champaca by using in vitro assay. Extraction was carried out with ethanol by using Soxhlet apparatus. The invitro antioxidant activity of ethanol extract has been investigated by 1, 1-diphenyl, 2-picryl–hydrazyl free radical (DPPH) method. The ethanol extract exhibited maximum antioxidant activity. The results have been compared with the standard ascorbic acid.

Anti-Tumor and Anti-Oxidant Activity of Ethanolic Extract of Epipremnum Aureum Linn. Leaves against DAL Induced Tumor in Swiss Albino Mice

Neoplasia literally means the process of “new growth,” and a new growth is called a neoplasm. The term tumor was originally applied to the swelling caused by inflammation. Neoplasms also may induce swellings, but by long precedent, the non-euplastic usage of tumor has passed into limbo; thus, the term is now equated with neoplasm. Oncology (Greek oncos = tumor) is the study of tumors or neoplasm’s. Cancer is the common term for all malignant tumors. The present study was designed to investigate the anti-tumor activity of ethanolic leaves extract of Epipremnum aureum Linn. and evaluated by in-vitro and in-vivo experimental models. To achieve objectives, EEEA was subjected to phytochemical screening and tested for oral toxicity test. The in-vitro study was carried out by means of MTT assay and Trypan blue dye exclusion assay using DAL cell lines. The in-vivo anti-tumor activity was evaluated against DAL tumour bearing mice by liquid tumour models. Preliminary phytochemical screening was confirmed the presence of flavonoids, glycosides, tannins, phenolic, steroids, and triterpeniods etc. EEEA showed good cytotoxic effect on DAL cell line in MTT assay and Trypan blue dye exclusion assay. Oral administration of EEEA in tumour bearing mice for 14 days, showed significant reduction in the percent increase in body weight, tumour volume, tumour weight, viable cell count when compared to the untreated mice of the DAL control group. The restoration of the haematological parameters towards the normal control was also observed. The results suggested that the EEEA exhibits significant anti-tumor activity towards both methods. The DAL-bearing mice orally administered leaves of Epipremnum aureum Linn., at 200 and 400 mg/kg body weight showed significant change in the average life span compared to animals of the tumor control group. The percentage increase in body weight, tumor cell volume, and number of viable tumor cells were found to be significantly less than the tumor control animals, indicating the anti-tumor nature of the extract.

Total Phenol and Antioxidant Activity of Ethanol Extract and Water Extract from Claw Uncariaa gambir Roxb

Uncaria gambier Roxb is a plant from the Rubiaceae family, belongs to the Bajakah group and has been used empirically in the treatment of various types of diseases in the community. Besides being one of the industrial plants and export commodities produced in Sumatra and Kalimantan, gambier leaves is also used frequently in Indonesia, both for the pharmaceutical and cosmetic industries. As a result, market demand both nationally and internationally have increased. The aim of this research are determination of antioxidant activity of ethanol extract and aqueous extract of gambir claw using 1,1-diphenil-2-picrylhydrazyl (DPPH) and determination of total phenol using Calcetau folin reagent. Gambier plant samples were taken in the forest area around the Tanjungpura University campus, the claws were separated and dried at room temperature, then powdered and extracted by maceration using 70% ethanol pa and aquadest, then the extract was concentrated until it was ready to be tested for antioxidant activity and determination of total phenol. The total phenolic ethanol extract and aqueous extract of gambir claw were 224.66 mg GAE/g extract (GEA : Gallic Acid Eqivalent) and 299.08 mg GAE/g extract, respectively, while the antioxidant potential (IC50) was 39.566 μg/mL and 65.140 μg/mL, and the IC50 for comparison of Vitamin C was 7.02 μg/mL.

The variation in essential oils composition, phenolic acids and flavonoids is correlated with changes in antioxidant activity during Cinnamomum loureirii bark growth

Cinnamomum loureirii is one of the most important medicinal and aromatic plants. It is used by the food, perfumery, cosmetic, and pharmaceutical industries. The objective of this study was to examine the relationships between the chemical composition and antioxidant activity of essential oil (EOs) and ethanol extracts from 10 samples of the bark from C. loureirii trees (6–15 years old). Obtained results showed that C. loureirii bark EO content at 12–15 years old had high oil yields (4.52–5.48%). The major components in the EOs were trans-cinnamaldehyde (50.2–92.9%) and α-copaene (0.5–21.3%). The highest content of trans-cinnamaldehyde in bark EOs was obtained from 10 to 12-year-old trees, while the highest content of α-copaene was obtained at 13–15 years. The highest total phenolic (429.85 and 474.45 mg TAE /g DW) and total flavonoid contents (85.54 and 102.80 mg RE /g e DW) were obtained from the 13- to 14-year-old trees. Our results indicated that the EO and ethanol extract of 13- to 14-year-old bark showed the strongest antioxidant properties. Moreover, GC–MS, PCA and correlation analysis indicated that the changes in α-copaene, beta-caryophyllene, τ-muurolene, γ-muurolene, δ-cadinene and τ-muurolol were positively correlated with the changes in antioxidant activity in oils. Meanwhile, procyanidin B2 and hyperoside were the main contributors to the antioxidant activity of the ethanol extract from C. loureirii bark. This study is the first one that report the antioxidant potential and phenolic profile of C. loureirii plant. These results provide reference information for the rational utilization of C. loureirii resources and the harvest of C. loureirii bark as medicinal materials during the optimal period.

Total phenolic content, total flavonoid content and antioxidant activity of ethanolic extract of Rafflesia kerri Meijer, Lojing Highlands, Peninsular Malaysia

Rafflesia kerri Meijer belongs to genus Rafflesia, recognized as the biggest flower in the world. This species found distributed in tropical rainforest of Thailand and Peninsular Malaysia. The flower believed to have medicinal values and used as traditional herbs by aborigine ethnic group and locals. Herein, this study was conducted to evaluate the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of the flower by using several antioxidant assays, which includes 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH) and ferric reducing antioxidant power (FRAP). The extract of female ethanolic extract exhibited highest antioxidant activity in all of the assays (FRAP = 4642.844 ± 10.777 mM/mg) and median inhibition concentration (IC50 = 49.850 ± 2.179). The same extract showed the presence of phenolic and flavonoid content with respective value of (Gallic acid equivalent, GAE) of 1634.731 ± 20.465 mg/100g and Quercetin equivalent (QE = 817.000 ± 69.282 mg/g) respectively with significantly differences (p < 0.05) compared to male ethanolic extract. Thus, the flower is highly valuable not only for ecotourism industry but also has potent medicinal value for human health improvement.

Chemical Constituents and Antioxidant Activity of Leaves and Stems Extracts of Monteverdia communis (Celastraceae)

Species from the genus Monteverdia (Celastraceae) are widely used in traditional medicine for gastric disorders. Pharmacological and phytochemical studies focus on Monteverdia ilicifolia (“espinheira-santa”). Monteverdia communis is endemic and abundant in southeast Brazil, and no previous work was reported in literature involving its chemical aspects. Given the potential of Monteverdia species as sources of natural antioxidants, this work aimed to undertake a chemical study and evaluate the antioxidant activity of leaves and stems extracts of M. communis. A fraction enriched with the triterpenes epifriedelinol and 3-oxo-friedelin was isolated from the hexane extract of the leaves. The antioxidant activity of ethanol extracts of leaves and stems was significantly higher than butylated hydroxytoluene (BHT) and statistically equivalent to rutin (positive controls). A weak negative correlation with the total flavonoid content was verified for these extracts, suggesting that the antioxidant activity may be related to the presence of other compounds in addition to flavones and flavonols. The phytochemical screening of hexane and ethanol extracts from leaves and stems revealed their chemical profile, providing quality control data. The results allow the indication of M. communis as a source of bioactive terpenoids and a promising target for further studies to isolate and identify antioxidant compounds.

Chemical profiles of heated perilla meal extracts and their antioxidant activities

SummaryChemical profiles of aqueous or ethanolic extracts of 140, 170 and 200 °C‐heated perilla meal were identified by GC‐MS, and antioxidant properties of the extracts were observed via in vitro assays and in bulk oil or oil‐in‐water (O/W) emulsion. A total of 22 and 27 chemicals were found in aqueous and ethanolic extracts from non‐heated samples, respectively. As the heating temperature increased to 200 °C, the carbohydrate and derivative contents decreased significantly (P &lt; 0.05), whereas rosmarinic acid concentration decreased in both extracts. Ethanolic extracts possessed higher antioxidant activities than aqueous extracts based on the results of radical scavenging and ferric‐reducing antioxidant power assays and the Rancimat assay, but there were no significant differences among samples (P &gt; 0.05). In the case of O/W emulsions, aqueous extracts inhibited lipid oxidation more efficiently than ethanolic extracts at 50 °C. In particular, heat treatment decreased the antioxidant activities of ethanolic extracts and not aqueous extracts in the O/W emulsion system. Aqueous extracts are recommended in moisture‐rich emulsion‐based foods while ethanolic extracts are more suitable in a lipid‐rich environment for enhancing oxidative stability.

Phytochemical screening and antioxidant activities of ethanol extract and ethyl acetic extract of “sikam’s” barks (Bischofia javanica BL)

Sikam’s barks (Bischofia javanica BL) are used as seasoning by Simalungun People. People use it for nahi nasumbah. Nahi nasumbah is a kind of food that is made from fresh meat or undercooked meat. There have never been reports of food poisoning as a result of consuming it. This research was aimed to evaluate the phytochemical and antioxidant activities of ethanol extract and ethyl acetic extract of Bischofia javanica BL barks. The screening was performed for phenolics, flavonoids, saponins, steroids/terpenoids, and alkaloids. Antioxidant capacity was evaluated by DPPH Method. The study of phytochemical screening indicated that ethanol extract contains phenolics, flavonoids, saponins, and ethyl acetic extract contains steroids/terpenoids. The antioxidant capacity indicated that IC50 ethanol extract of Bischofia javanica BL barks was 1,658 ppm and IC50 ethyl acetic extract of Bischofia javanica BL barks was 147.553 ppm. The results show that ethyl acetic extract activity is stronger than ethanol extract.

COMPARATIVE IN VITRO ANTIOXIDANT ACTIVITY OF ETHYL ACETATE AND ETHANOL EXTRACTS OF CALLICARPA MACROPHYLLA

Objective: The objective of the study was to evaluate and compare the antioxidant activity of (EA and E) ethyl acetate and ethanol extracts of (CM) Callicarpa macrophylla.&#x0D; Methods: The physiochemical parameters were assessed according to guidelines given by the world health organization. The total content of phenols and flavonoids was assessed by Folin–Ciocalteu and aluminum chloride methods. In vitro, antioxidant activity was screened by (DPPH) 1, 1-diphenyl-2- picrylhydrazyl and(H2O2) hydrogen peroxide scavenging and reducing power assay.&#x0D; Results: The physicochemical parameters fulfilled the standards of WHO guidelines. Total phenol and flavonoid content were more in ethanol extract as compared to ethyl acetate extract of CM. The antioxidant activity of ethanol extract was further high as compared to ethyl acetate extract of Callicarpa macrophylla. The IC50 of Callicarpa macrophylla ethanol extract was less than the ethyl acetate extract. So, more antioxidant activity of ethanol extract compared to ethyl acetate extract of CM.&#x0D; Conclusion: Overall, both the extracts showed antioxidant activity and can be used further for diseases that can be managed using antioxidants. Ethanol extract possessed significant antioxidant effects than the ethyl acetate extract.

ANTIOXIDANT ACTIVITY OF SYZYGIUM AROMATIUM AND CINNAMOMUM VERUM SEED EXTRACTS

The present study was carried out for the evaluation of antioxidant activities of clover (Syzygium aromatium L.) and cinnamon (Cinnamomum verum L.). The research work was carried out at the Institute of Molecular Biology, University of Lahore. The samples of clovers and cinnamon were collected from Lahore and were crushed to prepared sample in n-hexan, and ethanol extracts for evaluation of antioxidant activities. The highest antioxidant activities of ethanol extract from cinnamon indicated that the cinnamon may be used as an active ingredient to control stress conditions of cells for stress avoidance. The alkaloids, coumarins, saponins, flavonoids, tannins, sterols, quinons, triterpenoids, anthocyans, leucoanthocyans and terpenoids were tested for both ethanol and n-hexan extracts which indicated that most of the components were found present in both of the plant species, which revealed that these herbal plant seeds may be used as potential medicinal plants.

Antioxidant activities of ethanolic extract of Annona muricata against different pro-oxidant induced lipid peroxidation in rat brain and liver

Antioxidant activities of ethanolic extract of Annona muricata against different pro-oxidant induced lipid peroxidation in rat brain and liver

Comparative Analysis of Phytochemicals and Antioxidant Activity of Ethanol Extract of Centella asiatica Leaves and its Nanoparticle Form

a peer-reviewed, open access online international journal which publishes original research papers. The journal welcomes submission from scholars and experts for possible publication from all over the world. The scope of the journal includes: Pharmaceutical research, chemistry and biochemistry of naturally occurring compounds, biological evaluation of crude extracts, ethnomedicine, traditional and complementary medicine, ethnopharmacology, biomedical research, Biotechnology, Evaluation of natural substances of land and sea and of plants, microbes and animals, pharmacognosy, bioavailability, clinical, pharmacological, toxicological studies and pharmacokinetics of phytochemicals, Isolation and characterization of compounds, structure elucidation, synthesis and experimental biosynthesis of natural Product as well as developments of methods in these areas are welcomed in the journal.

Phytochemical Screening and Antioxidant Activity of Hippobroma longiflora Extracts

This study aims to identify the secondary metabolite compounds and to test the antioxidant activity of ethanol extract, n-hexane fraction, and chloroform fraction of Hippobroma longiflora leaves. Extraction was carried out by the maceration method using ethanol. The resulting crude ethanol extract was then partitioned with n-hexane and chloroform. Each extract and fraction was then tested by phytochemical screening. Antioxidant activity testing was carried out using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The phytochemical screening test showed that the ethanol extract contained flavonoids, saponins, triterpenoids, and alkaloids; the n-hexane fraction contained steroids, and alkaloids; and the chloroform fraction contained flavonoids, steroids, and alkaloids. Analysis of the antioxidant activity revealed the following Inhibitory Concentration (IC50 values): 9.57 ppm of ethanol extract, 99.59 ppm of n-hexane fraction, 48.54 ppm of chloroform fraction, and 4.30 ppm of ascorbic acid. Based on these results, the antioxidant activity of the ethanol extract was more potential than the n-hexane and chloroform fractions, but smaller than ascorbic acid.

English

The current study is designed to investigate the phytochemical screening and to examine the potential antimicrobial, antioxidant activities of ethanol extract of Cucumis flexouses and Cucumis reticulatus. The ethanol extract of C. flexouses and C. reticulatus seeds were subjected to preliminary phytochemical screening for the confirmation of various phytochemicals and their total phenolic and flavonoid content is verified by the colorimetric method. Subsequently, antimicrobial activity of both extracts at the concentration of (25, 50, 100 mg/ml) against various microbial organisms was evaluated via the disc diffusion method by measuring zone of inhibition and estimating minimum inhibitory concentration (MIC). The antioxidant activity was assessed by ferric thiocyanate (FTC) and DPPH free radical scavenging method. The ethanol extract of Cucumis flexouses showed maximum antibacterial activity against gram-positive bacteria including Staphylococcus aureus (29.0±0.05mm), Bacillus subtilis (17.0±0.02 mm) followed by gram-negative bacteria Escherichia coli (22.0±0.05 mm) and Pseudomonas aeruginosa (15.04±0.34mm) respectively at 100mg/ ml concentration as compared to standard drug. Moreover, both the extracts showed no activity against fungal species Candida albicans. Similarly, significant antioxidant effects at 100mg/ml concentration (1.17±0.025) were also observed by FTC and (1.09±0.017) by DPPH method. The result of the current study depicts that ethanol extract of C. flexouses seeds possess significant in-vitro antimicrobial and antioxidant effects as compared to C. reticulatus seed extract and could be a good substitute for many infectious diseases

Evaluation of Hepatoprotective and Antioxidant Activity of Ethanolic Extract of Artabotrys zeylanicus Stem against Various Hepatotoxins Induced Hepatotoxicity in Albino Wister Rats

The objective of the present study was to investigate the antioxidant and hepatoprotective activity of ethanolic stem extract of Artabotrys zeylanicus against paracetamol (PCT), Ethanol (ETN) and Isoniazid and Rifampicin (IR) induced hepatotoxicity in Albino wister rats.&#x0D; Methodology: The material was dried in shade, they were powdered and extracted with ethanol. Preliminary Phytochemical tests were done. The hepatoprotective activity of the ethanol extract was assessed in Albino wister rats. PCT (3 g/kg), ETN (5 gm/kg) and IR (100 mg/kg) has enhanced the levels of various biochemical markers of hepatic damage like Serum Glutamic Oxaloacetic Trasaminase (SGOT), Serum Glutamic pyruvic transaminase (SGPT), Alkaline phosphatise (ALP), bilirubin. Antioxidant levels were tested in all the Hepatotoxins treated and untreated groups.&#x0D; Results: The various biochemical and Histopathological investigations done were Serum Glutamic Oxaloacetic Trasaminase (SGOT), Serum Glutamic pyruvic transaminase (SGPT), Alkaline phosphatise (ALP), Bilirubin, antioxidant activity by 1,1-diphenyl 2-picryl hydrazyl (DPPH), Nitro Blue Tetrazolium (NBT), Hyderogen peroxide (H2O2), lipid perioxidation, hyderoxil radical and nitric oxide. Treatment of ethanolic extract of stem of A. zeylanicus (100mg/kg, 200mg/kg and 400mg/kg body weight) has brought back the altered levels of biochemical markers to the near normal levels in the dose dependent manner. Ethanolic extract of A. zeylanicus were observed to inhibit oxidant stress with the maximum value of 71% and 62% at the concentration of 100 µg/mL. The crude ethanolic extract of A. zeylanicus had a calculated IC50 value of 62.2 and 63.25 μg/mL, which is nearly similar to the calculated IC50 value of the known antioxidant, ascorbic acid, ie 65.3 μg/mL. While the rats treated with AZ extract (100, 200 and 400 mg/kg) which were shown as reduction/absence of inflammatory cells, vascular congestion, cellular degeneration, necrosis and vacuoles. In contrast, the lower doses (100 mg/kg) of ethanolic extract of AZ stem shown low protection than at higher dose 400 mg/kg.&#x0D; Conclusion: Our findings suggested that A. zeylanicus ethanol stem extract possessed a potent antioxidant and hepatoprotective activity.

In vitro antioxidant activity of ethanol extract of soybeans (glycine max [l.] merill) seeds

Introduction: Soybean is widely grown for its edible bean. It is a legume that grows in the tropical, subtropical, and temperate climates of Nigeria. It has been shown to contain a number of antioxidants that are used in preventing and treating chronic diseases. Aims: The purpose of this study is to evaluate the in vitro antioxidant activity of ethanol extract of soybean seed using the following assays: DPPH (2,2 diphenyl-2-picryhydrazylhydrate) scavenging activity assay, hydrogen peroxide (H2O2) scavenging activity assay, inhibition of lipid peroxidation activity assay, reducing power capacity assay, and antioxidant enzyme assay, which include superoxide dismutase (SOD) and catalase activity assay. Materials and Methods: In the present study, the antioxidant activities of ethanol extract of soybeans seed were determined spectrophotometrically using methods that include 2, 2-diphenyl-picryl hydrazyl radical (DPPH) scavenging activity assay, hydrogen peroxide scavenging activity assay, inhibition of lipid peroxidation assay, reducing power activity assay, peroxidation assay, and catalase and SOD activity assays. Results: The result of the DPPH scavenging activity revealed that the soybean extract has an EC50 value of 1053.542 μg/ml, hydrogen peroxide scavenging activity with an EC50 of 420.1852 μg/ml, and the inhibition of lipid peroxidation of soybeans extract had an IC50 of 1168.771 μg/ml. The reducing power activity of the soybeans extract had an OD0.5 of 484U/mg, catalase activity of 0.12985 U/mg, and SOD activity of 0.004125 U/mg. The EC50/IC50/OD0.5 obtained for the standard butylated hydroxyanisol (BHA) was lower than those of the soybeans extract. Conclusions: The use of soybean as a source of natural antioxidants should be promoted since soybean component can inhibit lipid peroxidation and protect the human body from the oxidative damages by free radicals. Hence, the dietary intake of soybean can be linked to prevention and management of certain diseases.

Evaluation of anticancer and antioxidant activity of ethanolic extract of Ocimum sanctum on KB cell lines- an in-vitro study

Evaluation of anticancer and antioxidant activity of ethanolic extract of Ocimum sanctum on KB cell lines- an in-vitro study

Hepatoprotective and antioxidant activity of ethanolic extract of Artabotrys odoratissimus stem on rifampicin-induced hepatotoxicity in rats

Hepatoprotective and antioxidant activity of ethanolic extract of Artabotrys odoratissimus stem on rifampicin-induced hepatotoxicity in rats