Anion-exchange Paper Research Articles

ISOLATION OF RIBONUCLEOTIDE-PEPTIDE COMPLEXES FROM PASTEURELLA MULTOCIDA.

SummaryRNA was extracted with 10% NaCl from acetone-dried cells of Past, mul-tocida and deproteinized by treatment with the chloroform-octanol mixture. After alkaline hydrolysis, CMP, AMP, UMP and GMP were separated by anion exchange chroma-tography and paper ionophoresis. The nu-cleotides were tightly bound to peptide material. Nucleotide-peptide complexes were also detected in the TCA-soluble extract from Past, multocida.

CHROMATOGRAPHY ON ION EXCHANGE PAPERS. XII. THE ADSORPTION OF METAL IONS ON VARIOUS ANION EXCHANGE PAPERS--FURTHER RESULTS.

Abstract A simple procedure is described for improving the reproducibility of two-dimensional papergrams made by the solvent systems: phenol—water followed by butanol—propionic acid—water. The procedure involves control of the water content of the chromatographic paper prior to the addition of solvent. Some effects noted upon the variation of the water content of paper are described for some chromatographic patters of biochemical interest.

Chromatographic behaviour of light rare earths on anion-exchange paper in nitrate-methanol systems

The chromatographic behaviour of rare earths from La to Gd has been investigated in HNO 3-methanol systems on the anion-exchange paper DE-20. The possibility of separation of two or more rare earths from each other has been shown as a consequence of the favourable effect of high methanol concentrations on selectivity. A comparison was also made with results obtained by elution under the same conditions on pure cellulose CLR/1 paper. This paper possessed only limited adsorption properties and did not show any selectivity toward different rare earths. The fact that in the liquid-liquid extraction system with the liquid anion exchanger tri- n-octylamine no rare earth adsorption occurs into the organic phase suggests that the adsorption and selectivity shown by the anion-exchange paper is not attributable to a pure anion-exchange process.

Anion-exchange paper chromatography of metal ions

The migration of 26 ions on anion-exchange paper is studied with five different complexing solvents. The results are reported as a function of the sign of the charge on each ion determined electrochromatographically. Several possible separations are presented, but it is shown that the prediction of separations, knowing only the charge on several substances, is impossible. It is also demonstrated that ion-exchange column experiments and ion-exchange paper experiments do not necessarily yield identical results.

THE IODO-AMINO ACIDS IN THE THYROID GLAND IN THYROTOXICOSIS; A STUDY USING ANION-EXCHANGE RESIN AND HIGH-VOLTAGE PAPER ELECTROPHORESIS.

THE IODO-AMINO ACIDS IN THE THYROID GLAND IN THYROTOXICOSIS; A STUDY USING ANION-EXCHANGE RESIN AND HIGH-VOLTAGE PAPER ELECTROPHORESIS.

Chromatography on paper impregnated with ion exchange resins : IX. The adsorption of metal ions from HCI on anion-exchange resin paper, DEAE paper and aminoethyl-cellulose paper

The adsorption of metal ions from HCI onto SB-2 resin paper, DE-20 and AE-30 cellulose anion-exchange papers was studied and the mechanism of adsorption discussed considering the differences observed between the various exchangers.

Rapid enzyme assay technique utilizing radioactive substrate, ion-exchange paper, and liquid scintillation counting

A rapid enzyme assay technique is described in which a labeled anionic product is determined as the radioactivity remaining on anion-exchange paper as measured by direct liquid scintillation counting of the paper after elution of the labeled nonionic substrate from the paper with water by one of three methods: (A) descending chromatography, (B) water extraction, or (C) direct elution employing a Millipore filtration apparatus. Reactions catalyzed by galactokinase and hexokinase were employed as examples. The technique generally should be applicable to reactions catalyzed by enzymes converting uncharged materials into anionic materials. The technique should also prove useful in enzymic reactions in which the product can be separated readily from the reactant on anion-exchange paper by means of a suitable solvent or by electrophoretic means. Benzoic acid-7-C 14, galactose-1-C 14, and galactose-1-P, C 14 were counted with an efficiency of about 58% on DEAE-cellulose paper by liquid scintillation counting. Windowless gas-flow Geiger counting of radioactivity of these compounds on DEAE-cellulose paper gave satisfactory results but the efficiency of counting was only about 18%.

Chromatography of nucleotides on a new anion-exchange paper

Chromatography of nucleotides on a new anion-exchange paper

Ion-exchange paper chromatography of lanthanide ions : I. Anion exchange of lanthanide citrate complexes

The application of cellulosic anion-exchange papers to the separation of tracer amounts of rare earths has been investigated, using citric acid solutions as eluents. It was found most satisfactory to use diethylaminoethyl-cellulose paper with 0.026 M citric acid (pH 2.4) as eluent. Under these conditions a practical separation factor of 2.6 between europium and cerium was obtained. Slightly higher separation factors could be obtained by adding ethanol to the citric acid solutions.

Estimation of glucuronosides by paper chromatography

A method for the quantitative estimation of glucuronosides in urine is described. Glucuronosides are separated by paper chromatography of purified urinary extract on Ecteola anion exchange paper (Whatman ET-20) or by column chromatography on DEAE cellulose (Whatman DE-50 floc). Glucuronosides present in paper or column eluates are estimated by the naphthoresorcinol reaction. Concentrations of glucurono- sides in urine as determined by this method are considerably lower than those reported for other procedures not involving purification or separation of the material to be estimated.

Chromatography of uronic acids, sugar acid lactones, and glucuronosides on anion-exchange paper or deae-cellulose

For the separation of uronic acids, sugar acid lactones, and glucuronosides paper chromatography on anion-exchange paper, such as Whatman ET-20, Whatman DE-20, and Schleicher & Schüll Anionenaustauscher Papier with Dowex 2 X8 may be employed. Mixtures of alcohols, water, and organic acids or buffers were as mobile phases. In a similar manner, glucuronosides and glucuronic acid or glucuronic acid γ-lactone can be successfully chromatographed on columns of anion-exchange cellulose (Whatman DE-50 floc).

Chromatography of some barbituric acid derivatives on modified cellulose ion-exchange paper

Chromatography on a modified cellulose anion-exchange paper has been used to separate mixtures of certain 5,5-substituted barbiturates. Aqueous “solvents” were found to be unsatisfactory but organic solvents gave good resolution in a shorter time than with “ordinary” filter paper. Complete resolution of a mixture of Phenobarbitone, Barbitone, Butobarbitone and Quinalbarbitone can be achieved in 4 hours.

Lipid chromatography on anion exchange paper

Lipid chromatography on anion exchange paper

Les nucléotides libres de la glande thyroide de boeuf et de mouton

The composition of acid-soluble nucleotides in the thyroid gland of ox and sheep was studied by anion exchange chromatography and paper chromatography. No important qualitative and quantitative difference was found between the glands of the two species. The nucleotides: cytidine-5′-monophosphate, diphosphopyridine nucleotide, adenosine-5′-monophosphate, triphosphopyridne nucleotide, guanosine-5′-monophosphate, inosine-5′-monophosphate, uridine-5′-monophosphate, adenosine-5′-diphosphate, guanosine-5′-diphosphate, uridine-5′-diphosphate, adenosine-5′-triphosphate, guanosine-5′-triphosphate, and the conjugated nucleotides: uridine diphosphate-N-acetylglucosamine, uridine diphosphate-N-acetylgalactosamine, uridine diphosphate galactose, guanosine diphosphate mannose have been identified.

Uridine isomer (5-ribosyluracil) in human urine.

A substance isolated from human urine by anion exchange absorption and paper chromatography was found to correspond in its ultraviolet absorption spectrum and chromatographic mobilities with a uridine isomer (5-ribosyluracil) recently described as a component of yeast ribonucleic acid.

Studies on 5′-Phosphodiesterases in Microorganisms

During investigations conducted on the mode of degradation of ribonucleic acid by Microorganisms, it was found that one strain of Penicillium produces nucleoside-5′-monophosphates from ribonucleic acid. Identification of these nucleoside-5′-monophosphates was carried out by means of chromatography on anion exchange column, ultraviolet spectrophotometry, paper electrophoresis, and characteristic color reactions using orcinol, carbazole, and rosaniline. It is suggested that this strain contains 5′-phosphodiesterase (or 5′-phosphodiesterase system) which cleaves all of the 5′-phosphodiester linkages in ribonucleic acid thus giving rise to nucleoside-5′-monophosphates. It is a point of interest in this study to indicate the presence of 5′-phosphodiesterase for the first time in a common microorganism, as 5′-phosphodiesterase has been recognized only in somewhat particular materials such as snake venom.

Studies on 5'-Phosphodiesterases in Microorganisms

During investigations conducted on the mode of degradation of ribonucleic acid by Microorganisms, it was found that one strain of Penicillium produces nucleoside-5′-monophosphates from ribonucleic acid. Identification of these nucleoside-5′-monophosphates was carried out by means of chromatography on anion exchange column, ultraviolet spectrophotometry, paper electrophoresis, and characteristic color reactions using orcinol, carbazole, and rosaniline. It is suggested that this strain contains 5′-phosphodiesterase (or 5′-phosphodiesterase system) which cleaves all of the 5′-phosphodiester linkages in ribonucleic acid thus giving rise to nucleoside-5′-monophosphates. It is a point of interest in this study to indicate the presence of 5′-phosphodiesterase for the first time in a common microorganism, as 5′-phosphodiesterase has been recognized only in somewhat particular materials such as snake venom.