In a routine optical remote sensor, there is a contradiction between the two requirements of high radiation sensitivity and high dynamic range. Such a problem can be solved by adopting pixel-level adaptive-gain technology, which is carried out by integrating multilevel integrating capacitors into photodetector pixels and multiple nondestructive read-outs of the target charge with a single exposure. There are four gains for any one pixel: high gain (HG), medium gain (MG), low gain (LG), and ultralow gain (ULG). This study analyzes the requirements for laboratory radiometric calibration, and we designed a laboratory calibration scheme for the distinctive imaging method of pixel-level adaptive gain. We obtained calibration coefficients for general application using one gain output, and the switching points of dynamic range and the proportional conversion relationship between adjacent gains as the adaptive-gain output. With these results, on-orbit quantification applications of spectrometers adopting pixel-level automatic gain adaptation technology are guaranteed.