Acute Postpartum Endometritis Research Articles

ОПЫТ ЛЕЧЕНИЯ ПОСЛЕРОДОВОГО ЭНДОМЕТРИТА У КОРОВ

The purpose of the study is to conduct a comparative analysis of the therapeutic effectiveness of a local antiseptic drug based on tea tree essential oil for acute postpartum catarrhal endometritis in cows. The study was conducted at the Department of VNB, Obstetrics and Physiology of Farm Animals of the Krasnoyarsk State Agrarian University, on the basis of the dairy farm of the Balakhta District of the Krasnoyarsk Region. The object of the study is red-motley cows. Research methods: obstetric and gynecological medical examination of breeding stock; the diagnosis of acute postpartum catarrhal-purulent endometritis was established during a clinical and gynecological examination of fresh cows, which inclu-ded thermometry, rectal and vaginal examination. The diagnosis was considered confirmed when pathological discharge of a catarrhal nature appeared from the uterus during rectal massage. Two experimental groups of fresh cows were formed according to the principle of analogues (based on live weight, age, lactation, clinical manifestation of the form of acute endometritis). In the 1st experimental group, scheme № 1 was used: a 25 % oil solution of tea tree essential oil was administered intrauterinely in a dose of 20 ml daily once a day until clinical recovery; eleovit 8 ml was administered intramuscularly on the 1st and 5th days of treatment; uteroton 10 ml was administered intramuscularly daily once a day for 3 days; 0.05 % chlorhexidine solution 200 ml – intravaginally; rectal massage of the uterus once a day. In the 2nd experimental group, treatment regimen № 2 was used, which differed from regimen № 1 by using a 50 % oil solution of tea tree essential oil in a similar dosage. The best therapeutic effect in the treatment of acute catarrhal endometritis was obtained using scheme № 2 using a 50 % oil solution of tea tree essential oil. Du¬ring the seven-day course of treatment, therapeutic effectiveness was obtained in 60.0 % of cases; prolongation of the course of therapy led to the recovery of all animals in this experimental group. The ave¬rage duration of treatment for the group was 8.0 days.

The effectiveness of vaccination in acute postpartum endometritis of cows

The article presents the results of studies of the vaccine against infectious mastitis and acute postpartum endometritis of cows inactivated. The specific efficacy of the vaccine was determined by the number of cases of cows with endometritis. The disease was recorded in 14.2% of the experimental and 52.5% of the animals in the control group within a month after calving.An experiment was conducted comparing the effectiveness of two immunization regimens, the first of which provides for primary vaccination of pregnant animals, the second — the beginning of immunization of heifers from 6–8 months of age, followed by a single injection every 6 months at a dose of 3 ml. During the experiment, endometritis was detected in 10% of cows in the 1st and 2nd experimental groups, which is 3.7 times lower than in animals in the control group (36.6%). Thus, it was established that both animal immunization schemes are effective in combating the incidence of calving cows with endometritis.When studying the intrauterine secretions of cows with endometritis, a significant effect of immunization on the species composition of pathogens was established. Thus, in cows of the control group, E.coli with endometritis was isolated 3.4 times more often than in animals of the experimental group. A similar pattern was observed for other microorganisms: the number of S. aureus isolates detected was 2.2 times less in the experimental group, S. agalactiae — 15.0 times, S. dysgalactiae — 2.0 times, S. uberis — 6.0 times, S. pyogenes — 3.0 times, K. pneumoniae — 3.4 times less in comparison with the control group.

Assessment of complex treatment schemes for cows with postpartum endometritis and the efficiency of their subsequent insemination

Purpose: сomparing the effectiveness of the proposed protocols for the treatment of cows with acute postpartum endometritis and establishing the effectiveness and the terms of their subsequent insemination.Materials and methods. 47 Cows of the Holstein-Frisan breed, which are on the binding content, were examined. All these animals were provided with obstetric care. As a result of their clinical inspection, 16 goals with an acute form of postpartum endometritis were revealed. Of these animals, two groups of 8 goals formed in each on the principle of pairs of analogues. When making a diagnosis, anamnesis, clinical signs of the disease and the results of an ultrasound of the study were taken into account. The first group included animals whose treatment regimen included intrauterine administration of Tilocar and intramuscular Uteroton. In the second group of cows, a 0.5 % Novocaine solution was additionally injected intravenously, according to the instructions. Results. The study revealed that the animals in the second group recovered three days faster (11.13±0.39 days) than the first group (14.25±0.37 days). We used the odds ratio. The second group of cows, where an additional 0.5 % of the Novocaine solution was administered, was found to be 2.78 times more likely to become pregnant than those treated without it.

Analysis of the effectiveness of treatment of acute postpartum endometritis in cows

The aim of the study is to study the effectiveness of the traditional treatment regimen for acute postpartum endometritis in cows on a farm. The study was conducted in the dairy industry. The object of the study was cows of a red-mottled breed. A retrospective analysis of the spread of obstetric and gynecological diseases in cows was carried out. It was found that endometritis is most common – in 72.1–77.9% of the total number of sick animals. The dynamics of the incidence of cows with acute postpartum endometritis was studied, which amounted to: in 2019 – 16.4%, in 2020 – 15.3% of calved cows. To identify the therapeutic effectiveness of the traditional endometritis treatment regimen, a group of seven cows with signs of catarrhal purulent endometritis was formed. The therapeutic effectiveness of the traditional treatment regimen for acute endometritis in young cows with a single application ensures the clinical recovery of animals in 85.7% of cases. However, 28.6% of treated cows subsequently had a subclinical form of endometritis, which reduces the effectiveness of the applied scheme to 57.1% and contributes to a 7.7% prolongation of service life.

BACTERIAL ENDOMETRITIS OF COWS AND THE CURRENT STATUS ITS ETIOTROPIC THERAPY

In the article a literature review related to the etiology, epizootology, pathogenesis, clinical symptoms of endometritis in cows and modern means of its etiotropic therapy is provided. Postpartum endometritis is considered one of the most common diseases in cows, causing great economic losses to dairy operators, due to the increase in the number of unfertilized cows after repeated artificial insemination, the increase of the service period and the percentage of culled cows, treatment costs, decrease in milk yield, etc.
 The main role in the etiology of endometritis in cows in the postpartum period is assigned to bacterial opportunistic microflora. In the etiology of endometritis, the basic role is played by Staphylococcus spp., Streptococcus spp. and Escherichia coli. Other bacteria can cause endometritis in cows, such as: Actinomyces pyogenes, Fusobacterium necrophorum, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Prevotella spp., Bacteroides spp. In many cases, acute postpartum endometritis in cows is caused by the association of microorganisms, especially Escherichia coli with streptococci and staphylococci.
 Microorganisms penetrate the mucous membrane of the uterus, their toxins and enzymes destroy nerve endings and capillaries, what leads to a reaction in the form of an inflammatory process, what is localized mainly in the surface layers of the endometrium and in the inter-glandular connective tissue. The development of endometritis depends on the immune response of the cow, as well as on the type and number of bacteria that colonize the endometrium.
 According to the kind of the inflammatory process, endometritis in cows are classified into: purulent; serous; catarrhal; serous-catarrhal; fibrinous; catarrhal-purulent. According to the passing of the disease, endometritis are acute, subacute, less often - chronic.
 Diagnosis of endometritis includes collection of anamnestic data, general clinical exploring of the animal and special gynecological (vaginal and rectal) testing.
 The basis of the treatment of endometritis in cows is etiotropic therapy, what involves by the use of antimicrobial drugs that affect the cause of the disease, and is used to stop the reproduction of opportunistic microflora in the uterine cavity and normalize the microbiocenosis. For the etiotropic therapy of endometritis in cows, drugs for intrauterine administration and drugs for systemic treatment in the form of a solution or suspension for injections are used.

Resistance to antimicrobials of the microflora extracted in acute postpartum endometritis in cows

In the article, the authors presented data on the species structure of the microflora isolated from cows with acute postpartum purulent-catarrhal endometritis. In acute postpartum endometritis, representatives of 8 genera of microorganisms were isolated in washings from the cervical canal. Identification of isolates to the species showed that Streptococcus pyogenes, Escherichia coli and Staphylococcus aureus were most often isolated from sick cows. These microorganisms were more common in associations: Escherichia coli + Streptococcus pyogene; Staphylococcus aureus + Streptococcus pyogenes. And Proteus vulgaris + Staphylococcus epidermidis. The disk diffusion method tested Streptococcus pyogenes, Escherichia coli and Staphylococcus aureus for resistance to 9 pharmacological groups of antimicrobial agents. In total, 48 isolates of 15 antibacterial drugs were tested in the work. The studied isolates of microorganisms showed multiple drug resistance. The authors revealed resistance to drugs of three or more pharmacological groups. Microorganisms showed high resistance (80% or more of isolates that did not show growth zone retardation) to drugs: neomycin (aminoglycosides) and benzylpenicillin (penicillins) - Staphylococcus aureus and Escherichia coli; vancomycin (glycopeptides), polymyxin (polymyxins) - Streptococcus pyogenes and Escherichia coli; ampicillin (penicillins), tetracycline (tetracyclines), cefazolin (cephalosporins), ciprofloxacin (fluoroquinolones) - only Staphylococcus aureus; lincomycin (lincosamides), tylosin (macrolides) - only Escherichia coli; streptomycin (aminoglycoside) - only Streptococcus pyogenes.

Effectiveness of the preparation «activiton» in acute post-natural purulent-catarial endometritis of cows

The article presents the results of a study of the effectiveness of the drug "Aktiviton" in the treatment of acute postpartum purulent -catarrhal endometritis of cows. The object of research were 15 cows of the Holstein breed aged 2 to 4 years, live weight 600-700 kg, owned by APK Food Program LLC, Mamadyshsky district of the Republic of Tatarstan. Animals were divided into 3 groups of 5 animals each. The control group consisted of clinically healthy animals in the postpartum period, the experimental groups included animals with acute postpartum catarrhal-purulent endometritis. In the treatment of endometritis, the following drugs were used: ceftivet, flexoprofen 10%, sinestrol 2%, uteroton, nioxytil forte. The treatment regimen used for animals of the 2nd experimental group was improved by the inclusion of "Activiton". Before treatment, the following clinical signs were observed in the experimental groups: edema and hyperemia of the external genitalia, the release of catarrhal-purulent exudate. Rectal examination revealed an increase in the size of the uterus, stiffness, pain and consistency. On the echogram, thickening of the walls of the uterus and consistency, heterogeneity of the mucosa, diameter of the body and horns, and wall thickness were noted. After treatment, an improvement in the general condition of the animals, the absence of a specific odor, and the release of exudate from the genitals were observed. During rectal examination, the size of the uterus corresponded to physiological parameters, its rigidity was observed, there were no fluctuations and soreness. On the echogram, the absence of thickening of the walls of the uterus, the homogeneity of the mucosa, the diameter of the body and horns, and the thickness of the walls were noted. In animals of the 1st experimental group, an improvement in the condition of the genital organs was observed on the 6th day after the start of treatment, however, in the cows of the 2nd experimental group, these changes were recorded on the 4th-5th day.

Microbiocenosis of the genital tract in cows with acute postpartum endometritis

Microbiocenosis of the genital tract in cows with acute postpartum endometritis

Совершенствование профилактики и терапии острых послеродовых эндометритов и маститов у коров

One of the limiting factor in the growth of productivity of dairy farming in the Russian Federation is the widespread among the breeding stock of pathologies of the reproductive organs and the mammary gland, most often of an inflammatory nature. Complex therapy of acute postpartum endometritis using Argumistin® 0.005 % showed 100 % efficiency, with a treatment duration of 7,8±0,33 days. Pregnancy from the 1st insemination was 58.4 %, which is 8,6; 15,8 and 7,2 % higher than after the use of Endometramag-Bio®, Hinasept-Gel and Ceftonite, respectively. The prophylactic efficacy of Argumistin® exceeds the efficacy of Endometramag-Bio® and Hinosept-Gel by 5,6 % and 11.1 %, respectively. The duration of the service period in the first experimental group was 42,5±4,22 days, which is 7,1; 18,9 and 35,9 days less than in other groups. Pregnancy from the 1st insemination in the group where Argumistin® was used was 3,8 and 20,6 % higher than in the groups where prophylactic treatment was carried out with Endometromag-Bio®, Hinasept-Gel, and also by 38,9 % more than the negative control. In the treatment of subclinical postpartum mastitis, the effectiveness of Argumistin® with a colloidal silver content of 0,005 % was 100,0 %, with a duration of treatment of 3,70 days.

EFFECT OF ANTIMETRIMAST ON MORPHOLOGICAL AND IMMUNOBIOCHEMICAL BLOOD VALUES OF COWS IN TREATMENT OF ACUTE POSTPARTUM ENDOMETRITIS

The article features the results of the morphological, biochemical and immunological studies of cow’s blood in the treatment of acute postpartum endometritis using antimetrimast. Complex treatment of cows with acute postpartum en-dometritis provides for the intrauterine administration of antimicrobial agents and intramuscular injections of the drug antimetrimast administered on three occasions with a 24-hour interval, starting from the first day of the therapeutic course, at a dose of 10 ml. The treatment is accompanied by the clinical recovery in 90.9% of animals, decrease in abnormalities of inflammatory nature, processes of lipid peroxidation, activation of the humoral and cellular component of immunity, enzymatic and non-enzymatic components of antioxidant protection.

PSIX-17 Cytokine profile of cow blood in the treatment of acute postpartum endometritis with recombinant α -, γ-interferons

Abstract The aim of the study was to research the cytokine profile of cow blood in the treatment of acute postpartum endometritis with the use of recombinant α-, γ-interferons. Animals of the first group (n = 9) with a diagnosis of acute postpartum endometritis were intramuscularly injected with propranolol hydrochloride, denatured emulsified placenta, and nioxityl. Propranolol hydrochloride was administered for 4 days at a dose of 10 ml/animal at 24-hour intervals. The denatured emulsified placenta was injected subcutaneously on days 1-5-9 at a dose of 25 ml /animal. Nioxityl was injected intrauterine at a dose of 150 ml three times with a 48-hour interval. Cows of the second group (n = 11) with the same diagnosis were additionally injected intramuscularly with bovine recombinant α-, γ-interferons three times in 1–3 days at a dose of 5 ml/animal, 1 cm3 of which contains at least 1x104 IU/cm3 of the total antiviral activity of bovine recombinant α-, γ-interferons. Blood samples are taken from all groups before and at the end of the experiment. Blood samples are examined for the content of interleukin-2 (IL-2), interleukin-4 (IL-4), tumor necrosis factor alpha (TNFα), interleukin-10 (IL-10) using Bovine Elisa Kit Clood-Clone Corp (USA). The therapeutic effectiveness in the first group was 77.8%, in the second - 90.9%, which is 13.1% more. At the end of the course, the level of IL-2 decreased by 42.5% (43.5±4.2 pg/ml, P < 0.01), TNF-Α by 9.1% (457.9±34.6 pg/ml), the level of IL-4 increased by 14.8% (44.2±3.5 pg/ml, P < 0.05), IL-10 by 56.6% (35.7±2.8 pg/ml, P < 0.01). In the second group, the level of anti-inflammatory cytokines decreased: IL–2 by 48.7% (38.8±1.6 pg/ml, P < 0.01), TNFα by 26% (372.5±17.6 pg/ml, P < 0.05) and increased anti–inflammatory cytokines: IL-4 by 46.2% (56.3±4.1 pg/ml, P < 0.001) and IL-10 by 80.3% (41.1±3.5 pg/ml, P < 0.001), which indicates a decrease in the inflammatory response.

Microflora of the contents of the uterus of cows with acute postpartum purulent-catarrhal endometritis and its sensitivity to antibacterial preparations

The microbiome of the uterus of cows with acute postpartum purulent-catarrhal endometritis is represented by Streptococcus faecium, Citrobacter freundii, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, Proteus vulgaris. Conditionally pathogenic microflora was predominant. Of all the tested praparations (Geomytsin, Enroflon, Mitrek), the highest zone of growth inhibition to most of the isolated microflora isolates was noted in the preparation Mitrek. The growth inhibition zone in Proteus vulgaris was 28.22 ± 2.37 mm, Staphylococcus aureus - 26.34 ± 1.34 mm, Streptococcus faecium - 33.0 ± 2.13 mm, Escherichia coli - 25.52 ± 1.55 mm, Citrobacter freundii - 21.4 ± 0.00 mm, Staphylococcus pyogenes - 19.09 ± 0.66 mm.

Effect of silver and dimethyl sulfoxide nanoparticles on antibiotic resistance of microorganisms in the treatment of purulent-catarrhal endometritis in cows

The increase in the production of livestock products, along with the improvement of the breed and productive qualities of animals, is closely related to the state of reproduction of the herd. The main factor holding back the reproduction of the herd to a large extent is the presence of acute postpartum endometritis. The study was carried out in order to study the species composition of microorganisms, the change in their antibiotic sensitivity during therapy after generic purulent-catarrhal endometritis of cows from 2019 to 2020. in the economy of the Novosibirsk region during the period of mass calving with a preparation containing silver nanoparticles in comparison with an antibacterial preparation. The animals were divided into experimental and control groups on the basis of analogs. The experimental group (n = 42) was injected intrauterinely with the drug, where the complex of drugs argovit, Dimexide in the form of 10% and 5% aqueous solutions at a dose of 0.3 ml / kg of live weight was used as an antibacterial drug once every 48 hours, and uteroton was intramuscularly 0 , 02 ml / kg live weight, once, once every 48 hours. The cows of the control group (n = 42) were injected with fish oil intrauterinely (0.3 ml / kg of live weight) with oxytetracycline hydrochloride (40 mg / kg of live weight), once every 48 hours and intramuscularly uteroton 0.02 ml / kg of live weight , once, once every 48 hours. The research results showed that the use of the drug argovit containing silver nanoparticles + DMSO caused a pronounced increase in antibiotic sensitivity in the isolated microflora in postpartum purulent-catarrhal endometritis of cows.

Rational methods of treatment of cows with acute postpartum purulent-catarrhal endometritis

The comparative therapeutic efficacy of “Enroflon®” and “Geomycin®F”, which have foaming properties, for the treatment of cows with acute purulent-catarrhal endometritis, is considered. Faster clinical recovery and uterine involution occurred in the experimental group of cows treated with “Enroflon®”. The recovery time of the animals was 1.19 days shorter compared to the use of “Geomycin®F”. For 90 days of the experiment, fertilization occurred in 100% of cows with an insemination index of 1.56 after treatment of animals with “Enroflon®”. Whereas after the application of “Geomycin®F” fertilization after insemination was 88.88% with an insemination index of 2.12.

РАЗРАБОТКА НОВОГО СРЕДСТВА ДЛЯ ЛЕЧЕНИЯ ВЫСОКОПРОДУКТИВНЫХ КОРОВ ПРИ ОСТРОМ ПОСЛЕРОДОВОМ ЭНДОМЕТРИТЕ

The article presents research results obtained during the development of a new treatment for highly productive cows with acute postpartum endometritis, which was performed at the Department of obstetrics, surgery and physiology of domestic animals the Don State Agrarian University in Persianovka settlement Oktyabrsky district, Rostov region. It was found that once in the affected cavity, the finished drug is completely absorbed and has a General and local effect on the affected tissues and organs. Animals of the first group were injected with the created enzyme – based drug at the following ratio of components, wt.%: dioxidin – 30.0, iodopovidone – 2.0 g, alantoin – 10.0 g, aloe extract – 5.0, retinol palmitate – 30.0 (300,000 IU), tocopherol acetate – 8.0 (120 mg), trypsin (20 thousand u), chymotrypsin (20 thousand u), rennin (20 thousand u) propranolol – 10.0, glycerin – 5,0, vaseline oil (base) - 42 ml. Animals of the second group were given a ready-made veterinary drug "Nioxitil" with the composition: oxytetracycline, tylosin tartrate, nitroxoline. To compare the therapeutic effectiveness of the proposed drug with the already approved drug, 2 groups of animals with 10 heads were formed on the principle of analogous pairs with postpartum endometritis. When using the test drug, the improvement of the General and local condition of the animal occurred on the 6th day from the start of the drug, the General therapeutic course was 11 days. The high therapeutic effectiveness of the experimental drug was noted.

EVALUATION OF METHODS OF TREATMENT OF COWS with POST-Partum ACUTE PURULENT-CATARAL ENDOMETRITIS

Clinical studies and observations found that 42.39% of cows after calving had inflammatory diseases of the uterus. Subclinical (23.19%) and acute forms of endometritis (12.86%) were dominant. Microbiological studies of the contents of the uterus of cows with acute postpartum purulent-catarrhal endometritis showed that the microflora is represented by Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Streptococcus faecium. After a course of therapy in cows with acute purulent-catarrhal endometritis with various preparations, restoration of sexual cyclicity was noted in all animals to which Enroflon was administered. After the treatment with Lexoflon, the manifestation of sexual cycles was recorded in 88.89% of females. After the treatment with Enroflon, fertilization occurred in 100% of the animals of this experimental group with an insemination index of 1.78. Fertilization of cows after the treatment with Lexoflon was 66.67% with an insemination index of 2.67.

CHANGES IN BIOCHEMICAL COMPOSITION OF BLOOD IN THE TREATMENT OF POSTPARTUM ENDOMETRITIS IN COWS

Postpartum complications are recorded in 40–80 % of cases of calving animals. A prerequisite for this is a metabolic disorder that affects the morpho biochemical status of the body. The use of drugs based on probiotic microorganisms for the treatment and prevention of obstetric and gynecological pathology is a promising direction. Therefore, the aim of our research was to study the changes in hematological parameters in the treatment of acute postpartum endometritis in cows with the use of a new probiotic drug. We developed drug Fometrin suppositories is a light yellow color, with a content of dry biomass of probiotic cultures of cells. Fometrin the drug were injected 3 or 4 candles of the first and second experimental groups of cows, respectively, 1 per day, intrauterine, for 5–7 days, also used the PDE to 20 ml and Uteroton 10 ml, 3 times in 48 hours. For treatment of control animals used Ichglukovit, Oksilat and PDE in accordance with the instructions for use. As a result, the largest number of recovered animals (80 %) was in the second experimental group, the independence period was 28 % and the service period was 31 % and the index of insemination by 15 % than in control. The use of endometritis therapy regimen with the drug Fometrin in comparison with traditional treatment, manifested a decrease in the content of leukocytes by an average of 26.9 % and an increase in the number of segmental neutrophils by an average of 36 %, as well as a more optimal level of nonspecific resistance of the body, determined by the ratio of lymphocytes to rod neutrophils, to the end of the experiment. After treatment there was an increase in iron content on average by 20.5 % and cholesterol by 27.6 % in the experimental groups of cows and the most pronounced changes in the indices of protein and mineral metabolism in the organism of animals second experimental group.

Sensitivity and Resistance of the Microbiota of Reproductive Organs and Mammary Gland of Cows to Anti-microbial Agents in Cases of Inflammation

This paper contains the results of complex microbiological studies (culture method, real-time PCR) of biological material from cows affected by inflammatory diseases of reproductive organs and mammary gland. Milk microbiota with underlying subclinical mastitis was represented by pathogenic (S. aureus, enteropathogenic E. coli), opportunistic bacteria (Staphylococcus spp., E. coli, E. faecium, Streptococcus spp., S. agalactiae, P. aeruginosa) and yeast-like fungi of Candida spp. in association. Combined infectious and inflammatory diseases of genital tract and mammary gland in cows resulted in the same types of microorganisms in microflora composition, which confirms information obtained by other researchers on associated microbiota during inflammatory processes. S. aureus, P. aeruginosa, E. coli, E. faecium, E. faecalis, S. epidermidis, S. saprophyticus, Bacillus spp., and yeast-like fungi of Candida spp. were obtained from milk samples from cows with acute clinical mastitis and in vaginal washes from cows with acute postpartum endometritis. In the course of defining the sensitivity to antibiotics and identifying genes of antimicrobial resistance, multidrug resistance was established in most part of obtained strains, which should be taken into account when planning treatment measures.

Hematological parameters that determine the course of labor and the postpartum period in highly productive Holstein cows

The aim of the study is to determine the effect of hematological parameters of cows on the course of labor and the postpartum period in highly productive Holstein cows. For this purpose, one group of cows was formed on the principle of analogue pairs in the amount of 30 animals with a gestation period of 7.5–8.0 months, followed by blood sampling from them using the Monovet system in vacuum test tubescontainers to determine morphological and biochemical parameters. Blood was taken 25–30 days before delivery in the morning 2 hours before feeding. Blood values were studied according to generally accepted methods using certified equipment. Subsequently, depending on the nature of the course of the birth, the animals were divided into two groups. The first group included 24 cows without pathology of the course of labor and the postpartum period, the second group included 16 cows with pathologies of the course of labor and the postpartum period. In the process of research, it was found that in 60.0 % of the cows the birth and the postpartum period passed without pathologies, and in 40.0 % of the birth and postpartum complications were revealed (retention of the placenta, acute postpartum endometritis, uterine subinvolution). A comparative analysis of the blood values of cows 25–30 days before birth the course of the labor process and postpartum period showed that in animals without pathology — compared to the animals with pathologies — the hematological parameters are considerably higher (hemoglobin content by 16.42 g/l, total protein by 10.96 g/l, albumin by 7.7 %, calcium by 0.98 mmol/l, glucose by 0.91 mmol, immunoglobin A by 59.77 mg/M), while the content of beta-globulins is lower by 8.51 % and that of enzyme AcT is lower by 25.94 units/l. A decrease in blood biochemical parameters contributes to the manifestation of the pathology of labor and the postpartum period. The data obtained can be used to develop an algorithm for the prevention of postpartum complications in highly productive cows.

Preclinical examination of complex antimicrobial preparation for treating endometritis in cows

According to a statistical study an average of 40 % of infertile animals have obstetric and gynecological pathologies. Among obstetric and gynecological pathologies, the most common is an acute postpartum endometritis, which make up from 37.8 to 41 %. Therapy and Pharmacology department of the Kuban State Agrarian University has developed a drug for the cows postpartum endometritis treatment. Conducted preclinical tests prove its harmlessness, as a result of which it belongs to hazard class 4. It should be noted that the biochemical blood parameters of rats in the experiment do not go beyond the reference values. The drug has a pronounced antimicrobial effect. MBC is from 7.8 mg to 31.2 mg while MIC is from 31.2 to 62.5 mg for such strains as S. aureus, E. coli, St. xylosus, Ps. aeruginosa, P. vulgaris. The developed drug does not have an irritating and sensitizing effect and exhibits a regenerative effect. Conducted experiments on the wound healing effect of the drug prove that this drug has a high regenerative effect. The average wound healing area was 20.4 % per day.