2nd Type Research Articles

GENOTYPIC ANALYSIS OF NODULE BACTERIA NODULATING SOYBEAN IN UKRAINE SOILS

Background. Distribution of root nodule bacteria of soybean in soils of Ukraine is the result of intensive cultivation of soybeans over the last 20 years. During the observation the structure of soybean rhizobia populations for the first time we have determined the strains which significantly differ in phenotypic properties from typical slow-growing bacteria of B. japonicum species previously described. These strains are characterized by high speed growth and we tentatively called them “stains with intensive growth”. The aim of our work was to investigate the genotypic properties of microsymbionts of soybeans with different rates of growth spreading in soils of Ukraine. Materials and methods. The 16S rRNA gene and intergenic 16S-23S rRNA region of six strains - typical representatives of soybean nodule bacteria with slow- and intensive growth-rates was carried out. The strains were picked up from different Ukrainian soils. Results. Analysis of the 16S rRNA nucleotide sequences showed the 100% similarity of slow-growing strains to B. japonicum USDA 6T and USDA 4 ones. This analysis proved propinquity of strains with intensive growth to the strain B. japonicum USDA 127 (USDA 123 group). Representatives of this group possessed increased saprophytic competence so as the examined strains. With use of restriction analysis of ITS intergenic region soybean rhizobia were divided among two ITS types: 1st ITS type - strains with intensive growth, 2nd ITS type - slow- growing strains. According to results of ITS-region sequencing soybean rhizobia form 3 reliably different clusters: two of which include slow-growing strains (group USDA 6 and USDA 4), and a third include soybean microsymbionts with intensive growth (USDA 123 group). Conclusion. On the basis of phenotypic and genotypic (16S rRNA and 16S-23S rRNA) analysis all of the investigated soybean strains of root nodule bacteria were related to the Bradyrhizobium japonicum species. The division of strains by the structure of the ITS-region into two genotypes corresponds to the division of strains into two physiological groups: the strains of an intense and slow growth.

Filamentary keratitis epidemiology

AbstractPurpose To analyze concomitant pathology contributing to filamentary keratitis.Methods We examined 38 patients (76 eyes) with filamentary keratitis. All of them were consulted by co‐specialists (internist, endocrinologist, reumatologist) with the object to reveal concomitant diseases. The treatment regimen included keratoprotectors, artificial tears, 0,1% dexamethasone, 0,05% cyclosporine. Besides, in case of decompensated concomitant disease patients were treated by appropriate specialist. Among investigative methods were: biomicroscopy, Shirmer test – 1, TBUT‐test. Follow‐up period was 3‐5 months.Results Among concomitant pathology we revealed:  Thyroid gland disfunction (hypothireosis) – 34,2 %  Rheumatoid arthritis – 21,1 %  Sjögren's Syndrome – 2,6 %  Diabetes mellitus of 2nd type – 7,9 %  Combination of rheumatoid arthritis and thyroid gland dysfunction (nodes against a background of euthyreosis) – 10,5 %  Combination of diabetes mellitus of 2nd type and thyroid gland dysfunction – 13,2 %  Neither endocrine nor rheumatoid diseases revealed – 10,5 %. Among them 1 patient after previous PRK. Shirmer test – 1 results were variable and no appropriateness with treatment regimen was found. TBUT‐test data before treatment were low (6‐9 sec.) with an increase up to 9‐11 sec. after the treatment.Conclusion 89,5 % of patients had endocrine pathology or rheumatoid diseases or their combination. With this in view, we may consider filamentary keratitis not as a form of severe dry eye syndrome, but as a separate dystrophic corneal disorder with dry eye as its symptom.

Application of generalized comb wavelet functions for angiogram image segmentation

The existing methods of angiograms processing do not provide the required speed and quality of segmentation, which determines the accuracy of diagnostic solutions. Therefore, to reduce the angiograms processing time a method has been developed for segmentation of the vessels illustrations on the 3098pangiograms, and analyze the result of segmentation by means of conversion with the generalized comb wavelet function at the vessels localization and the replacement of several processing levels by the one. The latter is achieved by the fact that the convolution with a generalized comb wavelet function is similar to using a set of bandpass filters. At the phase of segmentation results analysis of the vessels skeleton excretion was increased using morphological processing. The implementation of the developed method has allowed to reduce angiogram images processing time at 43% that is required to combine the diagnostic and therapeutic potential of the angiography method during a single procedure. Thus, the noise resistance characteristics may be changed as follows: the probability of the 1st type error is 1.22 times reduced, and the probability of the 2nd type error 1.14 times increases. As a result of experiments, it was shown that the developed method provides the vessels localization quality required for accurate singling out of the diagnostic parameters of heart disorders, which improves the accuracy of heart diseases diagnosing.

SAT0469 Treatment of Adult Juvenile Idiopathic Arthritis Patients with Biologic Agents. Data from the Czech National Registry

ObjectivesTo analyze the efficacy and safety of biologic agent in adult patients with juvenile idiopathic arthritis (JIA).MethodsATTRA is a Czech national registry of patients with different forms of chronic arthritis...

Estimation of Natural Recharge and Groundwater Build up in the Bandung Groundwater Basin Contributed from Rain Water Infiltration and Inter-aquifer Transfer

Bandung groundwater basin (GWB) is now in the serious crisis. It is basically composed of three aquifers, i.e.: (1) the uppermost unconfined aquifer, (2) the middle semi-confined aquifer and (3) the lower semi-confined aquifer. The groundwater (GW) crisis is due to over-exploitation especially from the middle semi-confined aquifer. In order to address the condition of GW resources in the region, estimation of GW recharge and GW build up are very important. This may help the GW management to overcome the present GW crisis. This paper will be devoted to share about the estimation of GW recharge and GW build up in the Bandung GWB contributed from direct rain water infiltration and inter-aquifer transfer that have been assessed by means of GW modeling. The 1st recharge can be assossiated with the vertical GW movement in the unsaturated zone, while the 2nd recharge was considered to be related with the GW flow in saturated intermediate to regional flow. The 1st recharge generally occures in the topographical high surrounding the Bandung basin, while the 2nd type can be predicted to occur along the GW flow path, i.e in the topographical high as well as in the regional GW flow. The 1st recharge would result in shallow GW system in the uppermost unconfined aquifer, while the 2nd recharge will result in the leakage into the middle semi- confined aquifer that is the most critical aquifer in the Bandung GWB.The conceptual model of natural recharge mechanism in the Bandung GWB was based on the model of natural recharge in the mountain front basin from Wilson (1980). The GW recharge and build up can be approached using GW modeling. The 1st component has been estimated based on 1D model of Richards (1931), whereas the estimation of 2nd component has been carried out based on aquifer leakage phanomen on the general 3D aquifer leakage model of Widodo et al (1999). Regional transfer between aquifers was performed by means of GW zone budgeting.Direct infiltration of rain water in Bandung GWB was estimated 5% of precipitation in the Bandung basin that builds up around 4.6 m3/sec additional GW. KBU is the most favourable GW recharge zone in Bandung GWB and contributes 2.6 m3/sec (57%) natural GW recharge, from KBS 1.5 m3/sec (32%) and from KBT 0.5 m3/sec (11%). In KBU the recharge zone was predicted at topographic level between 1000 and 1300 msl. The GW discharge zone in Bandung GWB can be attributed to the volcanic rocks at topographic level between 650 and 850 msl. The absolute discharge zone in the Bandung GWB is the River Citarum.

Social aspects multi-drug resistant tuberculosis

The comparative analysis of features of clinical symptoms, spectrum and the importance of burdening medico-social factors of a tuberculosis of lungs with primary MDR М . tuberculosis in comparison with a drug sensitive variant of disease is carried out. Presence of associations between burdening factors of disease and MDR М . tuberculosis is proved. We defined degree of risk of detection MBT with MDR at new cases tuberculosis of lungs with narcotism, a tobacco smoking, a syndrome of alcoholic abuse, stay in imprisonment places, a diabetes of 2nd type, hepatitises B, C, В + С.

Peculiarities of propagation of acoustic excitations through an imperfect 1D-superlattice

Abstract Virtual crystal approximation is adapted to address peculiarities of propagation of acoustic waves through a 1D ‘sandwich’ superstructure consisting of alternating layers of two types randomly substituted by foreign layers of the third type. Same-parity layers are of the same width and constitute a sublattice. Dependence of the lowest forbidden acoustic zone width of the described structure on concentrations of impurity layers in the two sublattices is numerically evaluated for longitudinal and transverse excitations. Values of substitute concentrations making the structure completely transparent prove to be independent of the relative widths of the 1st and 2nd type layers.

Experimental models of diabetes mellitus types 1 and 2 in rats: Regulation of activity of glycogen synthase by peptides of the insulin superfamily and by epidermal growth factor in skeletal muscles

The regulatory effect of peptides of the insulin hyperfamily--insulin, insulin-like growth factor (IGF-1), and relaxin, as well as of epidermal growth factor (EGF) on activity of glycogen synthase (GS) in rat skeletal muscles was studied in norm and in experimental diabetes mellitus of the 1st and 2nd types (DM1, DM2). In norm, peptides in vitro stimulated maximally the GS activity at a concentration of 10-8 M. The row of efficiency of the peptide action was as follows: insulin > IGF-1 > relaxin. In DM1 the basal GS activity did not change, while effect of insulin in vitro was decreased more sharply as compared with action of IGF-1 and relaxin at the 30th day of development of diabetes, i. e., the efficiency row was as follows: IGF-1 = relaxin > insulin. Administration of insulin in vivo did not restore sensitivity of the enzyme to the action of hormone in DM1. In DM2, the GS activity (both the total and active form) decreased. while the stimulatory effect ofpeptides and EGF on the enzyme was absent. Insulin introduced in vitro did not lead to restoration of the enzyme reaction. The conclusion has been made that the insulin resistance affects the basal GS activity in rat skeletal muscles as well as the regulation of the enzyme by peptides of the insulin nature and by EGF, which is more obvious in DM2, than in DM1.

福州上下杭社区（福建省）の住居類型とその変容に関する考察

This paper clarifies urban formation and transformation of Shangxiahang area in the city of Fuzhou. Fuzhou, which is known as Rong chen (Banyan castle), had developed as a port city since the period of Ming Dynasty after the decline of Quanzhou. Authors selected the old district of the port area of Fuzhou. There still exist two types of traditional house typeswhich are da-cuo and chai lan cuo (shou-jin-liao inQuanzhou city). Da-cuo is the form of quadrangle, si-he-yuan, but here in Shangxiahang area, we have the 2nd floor type of Da-cuo, which are very rare in Fujian district. Discussing relationships between house types of Da cuo and chai lan cuo leads to understand the development and transformation process of house types. This paper clarifies the characteristics of house types and their transformation process of Shangxiahang area in the city of Fuzhou.

Probleme ale genezei şi restaurării cupelor de tip kantharos de la Sâncrăieni / Research issues regarding the genesis and restoration of the Sâncrăieni kantharos-cups

Cu cele opt cupe cu picior şi toarte (kantharoi) şi şapte cupe hemisferice (mastoi), la care se adaugă două brăţări, o fibulă şi două monede, tezaurul de la Sâncrăieni reuneşte cel mai mare set de veselă din argint din Dacia perioadei târzii a Latène-ului. De altfel, prin greutatea lui excepţională (3.650 g), tezaurul devansează toate celelalte descoperiri dacice cu piese de argint. Diferenţele tehnologice şi stilistice dintre elementele consitutive ale cupelor de tip kantharos permit identificarea mai multor meşteri : (1) meşterul calotelor gravate (calotele cupelor nr. 4– 8) ; (2) meşterul cupei de import (nr. 1) ; (3) meşterul cupelor nr. 2– 3 şi al picioarelor de tip 3 (fig. 12 şi 13/ 2– 3) şi (4) meşterul picioarelor de tip 2 (fig. 12). Dacă meşterii 1, 3, şi 4 sunt locali, meşterul nr. 2 este greco-roman. De asemenea, se poate presupune o diferenţă cronologică de aproximativ o generaţie între meşterul nr. 1 şi meşterii 3 şi 4. În orice caz, colaborarea mai puţin armonizată a mai multor meşteri cu maniere diferite de ornamentare a determinat lipsa de unitate stilistică a elementelor constitutive ale cupelor de tip kantharos produse în mediul „ barbar” (cupele nr. 2– 8). Această lipsă de unitate stilistică, precum şi demontarea cupelor de la Sâncrăieni înaintea îngropării lor, a dat naştere unor dileme insurmontabile pentru restaurarea modernă. Din acest motiv, ar fi fost preferabilă evitarea intervenţiilor cu caracter ireversibil şi păstrarea elementelor constitutive ale cupelor de tip kantharos de la Sâncrăieni în starea în care au fost ele descoperite.

Kantharos cups in Sâncrăieni-hoard: restoration and manufacturing issues

The technological and stylistic differences of the kantharos cups elements in Sâncrăieni allow us to identify several distinct artisans: (1) the artisan of the engraved bowls (the bowls of cups no. 4-8); (2) the artisan of the imported cup no. 1; (3) the artisan of cups no. 2 and 3, probably of the 3rd type of stems as well and (4) the artisan of the 2nd type of stems. Artisans 1, 3 and 4 are local, whereas the 2nd one is Greco-Roman. One can also assume that there is a chronological gap between craftsman no. 1, on the one hand, and the 3rd and 4th, on the other – it’s probably a matter of one generation. In any case, the less than harmonious collaboration of a number of craftsmen with different manners of ornamentation, led to a lack of unity in style of the elements constituting the kantharos cups that were produced in a “barbaric” environment (cups no. 2-8). This lack of stylistic unity, as well as the disassembling of the cups in Sâncrăieni prior to their burial brought forth dilemmas that proved insurmountable to modern restoration. It would have been preferable, therefore, to avoid interventions of an irreversible nature and to preserve the separate elements of the kantharos cups in the state in which they had been discovered.

Interacting Many-Particle Extensive Quantum Mechanics

Recently there has been an interest inalternative solution methods to otherwise difficult or computationally expensive DFT density functional theory and many-particle physics theory and quantum mechanics. In previous articles we utilized transformation methods by which the nonlinearity of the extensive quantum mechanics was advantageously re-defined to a system of equations comprised of linear PDEs of the 2nd order Schroedinger equation type coupled to 1st order nonlinear PDEs of the transformations, thus obtaining simplified analytical mathematics solutions and computational alternatives for the many-body problem...in this article we derive transformations at the PDE level and its derivation from 2nd quantization briefly discuss other approaches.

Soil Environmental Quality Assessment on an Abandoned Industrial Land

With the rapid development of urbanization and industrialization in China, whether the abandoned industrial land can be reused has become the focus of land planning. So soil environmental quality assessment on the abandoned land is necessary. In this paper, the land of a discarded chemical plant in Chongqing, China was chosen as the study object. The concentrations of heavy metal were studied. Single Factor Pollution Index (SFPI) and Integral Pollution Index (IPI) were applied to assess the degree of soil pollution. The results revealed that the abandoned industrial soil in this work was slight polluted and could only be planned for the 2nd type of land utilization, including green land, commercial land, public facilities land, etc.

Mathematical modelling and control of the evolution of dynamic systems interacting with medium

The article proposes a new method of mathematical modeling evolutional systems interacting with medium. A cutting process or tribo-space formed in the contact area of two conjugate mechanical subsystems is considered as medium. The features of the medium depend not only on state coordinates of systems but also on trajectories. It is obtained that the parameters of the medium are performed as integral operators of equations like Volterra equations of 2nd type. The problems of control of evolutional systems are being analyzed.

Abstract 426: The molecular microenvironment critically affects the formation of isoDGR-dependent integrin binding sites in fibronectin

Abstract Fibronectin (FN) is an adhesive protein present in most body fluids and in the extracellular matrix of many tissues. This protein mediates a variety of cellular interactions with extracellular matrix and play important roles in haemostasis, thrombosis, inflammation, wound repair and angiogenesis. FN is constituted of three types of repeating homologous modules termed FN-I, FN-II and FN-III. Each module contains binding sites for molecules of the extracellular matrix and for cell adhesion receptors, such as integrins. Moreover, FN contains four NGR sites located in the: 5th type I, 2nd type II, 7th type I repeat, 9th type III repeats. We have previously shown that the NGR site of the 5th type I repeat (FN-I5) rapidly undergoes deamidation leading to formation of isoDGR. A deamidated FN fragment corresponding to this domain binds αvβ3, inhibits endothelial cell adhesion and proliferation and inhibits tumor growth in animal models. In the present study we have investigated the molecular mechanisms that regulate isoDGR formation in FN in vivo. We provide evidence to suggest that FN purified from plasma contains very low amounts of isoDGR (<3%) despite its long half life (48h). In contrast, using anti-isoDGR antibodies, we observed that isoDGR is formed in significant amounts in small FN fragments injected in mice and left to circulate for 4 or 6 h (>17% in 4 h). In vitro cell adhesion assays, antibody binding assays and integrin binding studies showed that FN deamidation can rapidly occur when FN is bound to microtiter plates, a condition known to cause conformational changes in this protein, but not with FN in solution. Rapid deamidation was observed to occur also with short fragments (FN-I5), either adsorbed on microtiter plates or in solution. These results suggest that the kinetics of NGR-to-isoDGR transition in plasma FN is slower than its clearance rate. Furthermore, the finding that conformational changes and/or protein fragmentation increase the kinetics of NGR-to-isoDGR transition suggest that changes in the molecular microenvironment of NGR sites, potentially occurring after FN deposition in tissues, could be critical for the formation of isoDGR integrin binding sites. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 426. doi:10.1158/1538-7445.AM2011-426

A versatile approach for modeling and simulating the tacticity of polymers

We are introducing a versatile computerized approach to model and simulate polymer tacticities using seven single-stage statistical models. The theory behind the models, e.g., Bovey's versus Price's, Bernoullian, 1st or 2nd order Markovian, enantiomeric types, and combinations thereof is explained. One of the models, "E-B gen", which can be used to produce four types of enantiomorphically controlled tacticities, and the pentad distribution for the model "E-M1" are reported here for the first time. The relations of chain-end controlled models to binary copolymerizations are discussed in detail, and equations for the conversion of tacticity based probabilities to reactivity ratios to obtain related n-ad distributions are presented. The models were applied to 20 polymers with exemplary tacticities found in the literature. A related software program ("Polytact") based on Microsoft's Excel has been designed to calculate all relevant characteristics of the polymer tacticity and to present them in graphical form in a user-friendly manner. The program can be used to produce graphs of the triad, pentad and sequence length distributions and a simulation of 50 monomer repeat units in the polymer for each of the seven models. One of the main intended uses of the program is to compare the computed n-ad distributions to those of experimental polymers obtained from NMR spectroscopy and to gain insight into the polymerization mechanisms.

Substrate-borne Vibrations as a Component of Intraspecific Communication in the Groundhopper Tetrix ceperoi

Substrate-borne vibrational signals used in communication of Tetrix ceperoi (Orthoptera: Tetrigidae) are described for the first time. Among all studied Tetrix species, T. ceperoi use the richest repertoire of vibrational signals. These signals consist of isolated pulses or pulses united into rhythmically repeated or irregularly repeated groups and are used in rivalry, pre-copulation, and copulation behaviour (1st, 3rd, and 4th structural types of signals), and are also used by single males or females (2nd type of signal). A 5th type of vibrational signal is produced by wing tremulation, which was unknown Tetrigidae. Based on the results of this study, the vibrational signalisation seems to play an important role in species-specific mate recognition of T. ceperoi. The significance of individual signals is discussed based on results of manipulative contact experiments.

Optoelectronic properties of sputter-deposited Cu 2O-Ag-Cu 2O treated with rapid thermal annealing

Cu 2O and two types of Cu 2O–Ag–Cu 2O (CAC) multilayered thin films were deposited on glass substrates using DC-magnetron sputtering. For CAC films, the mass thickness of Ag layer was controlled at 3 nm. After deposition, some of these films were annealed using a rapid thermal annealing (RTA) system at 650 °C, in order to create embedded Ag particles. AC films were used to study the clustering effect of Ag in Ar atmosphere, as well as for forming the 2nd type of CAC film by covering another Cu 2O layer on the annealed AC structure. A UV–VIS–NIR photometer, a Hall measurement system, and a I–V measurement system were used to characterize the optical and electrical properties of these films with and without RTA. The results show that 2-dimensional Ag layer can transform into many individual particles due to its high surface tension at annealing temperature, no matter when the annealing was carried out. For CAC films, without annealing, the optical transmission and the resistivity are decreased with the inserted Ag layer. After annealing, both the transmission and resistivity are increased, possibly due to the clustering effect of Ag layer. Most importantly, it is found that the embedded Ag particles can increase the light absorption in the NIR–IR region, which can increase photo-induced current.

Functional significance of polymorphism of ApoE and SOD2 genes in formation of chronic HCV infection

The analysis of gene polymorphism plays an important role in assessment of disposition to infectious diseases at the population and individual level. In this paper, the frequencies of allelic versions of apolipoprotein E and 2nd type superoxide dismutase genes and the corresponding genotypes were determined in healthy persons and chronic viral hepatitis type C patients of the Europeoid population of the Tomsk Region. For the analysis of polymorphism of these genes, we used modern genetic methods: polymerase chain reaction and polymorphism of lengths of restriction fragments. For the studied population, it was revealed that the polymorphism of apolipoprotein E and 2nd type superoxide dismutase genes correlates with the development of chronic viral hepatitis type C and with the fibrogenesis process.

Alvar forest of Harjumaa district

Harjumaa loometsad Alvar forests in Harjumaa district, NW and N Estonia, were studied. Eight community types were established: (i) Inula salicina-Potentilla fruticosa-Pinus sylvestris, (ii) Carex panicea-C. cespitosa-Pinus sylvestris-Betula pubescens, (iii) Filipendula vulgaris-Brachypodium pinnatum-Pinus sylvestris, (iv) Brachypodium pinnatum-Calamagrostis arundinacea-Picea abies-Pinus sylvestris, (v) Hepatica nobilis-Brachypodium pinnatum-Picea abies-Pinus sylvestris, (vi) Paris quadrifolia-Anemone nemorosa-Fraxinus excelsior, (vii) Mercurialis perennis-Picea abies, and (viii) Pulmonaria obscura-Viola mirabilis-Betula pendula-Picea abies. The 2nd community type represents the Sesleria alvar forest site type (FST) habitats, while communities of the 1st, 3rd and 4th types belong to the Calamagrostis alvar FST. These communities grow mainly on medium thick or thin Calcaric Skeletic Regosols, less frequently on thin Renzic Leptosols or on other types of soil. Other communities qualified by forest inventory as alvar forests factually belong to the boreo-nemoral Hepatica FST (communities of 5th type) and Aegopodium FST (communities of 6th, 7th and 8th types). Alvar forests are characterized by remarkably high species diversity deriving first of all from the number of field and moss layer species. The number of species in all layers, except moss layer, depends significantly from the community type reflecting in integrated form effect of communities' structure. Diversity of field layer species is positively affected by the ratio of carbon and nitrogen content in soil humus horizon, whereas the higher abundance of pine trees has negative effect on the species number in moss layer.