Abstract
ABSTRACTEight Lactobacillus strains, each intrinsically resistant to an antibiotic, were isolated from two commercial probiotic products. Whole-genome sequencing identified two efflux transporters, a multidrug and extrusion protein (MATE) efflux transporter, and LmrCD, which may contribute to their intrinsic antibiotic resistance and may therefore facilitate their survival in the intestinal microbiota following antibiotic therapy.
Highlights
Eight Lactobacillus strains, each intrinsically resistant to an antibiotic, were isolated from two commercial probiotic products
Eight Lactobacillus strains were isolated from two commercial probiotic products, and each was found to be resistant to an antibiotic of clinical importance
Lactobacillus strains were isolated by performing serial dilutions of two commercial probiotic products and plating onto DeMan-Rogosa-Sharpe (MRS) medium (SigmaAldrich, Ontario, Canada) supplemented with gentamicin (5 g/ml), streptomycin (10 g/ml), kanamycin (30 g/ml), aztreonam (30 g/ml), and ciprofloxacin (10 g/ml) and incubated anaerobically for 48 h at 35°C [3]
Summary
Eight Lactobacillus strains, each intrinsically resistant to an antibiotic, were isolated from two commercial probiotic products. Eight Lactobacillus strains were isolated from two commercial probiotic products, and each was found to be resistant to an antibiotic of clinical importance. We performed whole-genome sequencing (WGS) on these isolates to determine the nature of their resistance.
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