Abstract
Here, we report the genome sequences of Bacillus safensis RIT372 and Pseudomonas oryzihabitans RIT370 from Capsicum spp. Annotation revealed gene clusters for the synthesis of bacilysin, lichensin, and bacillibactin and sporulation killing factor (skfA) in Bacillus safensis RIT372 and turnerbactin and carotenoid in Pseudomonas oryzihabitans RIT370.
Highlights
The plant genus Capsium (Solanaceae) is of interest given its use in a number of herbal remedies by indigenous inhabitants throughout the world and the ability to synthesize the antibacterial metabolite and the active ingredient capsaicin (8-methyl-Nvanillyl-6-nonenamide) which is predominantly stored in the fruit of the plant [1, 2]
To gain some insights into bacteria that associate with the Capsicum spp., we embarked on a project as a laboratory exercise in the Fundamentals of Plant Biochemistry and Pathology course in the Biotechnology and Molecular Bioscience Program at the Rochester Institute of Technology to assess the bacterial diversity of bacteria associate with the fruits of Capsicum annuum and Capsicum chinense
This resulted in the isolation and identification of Bacillus safensis RIT372 and Pseudomonas oryzihabitans RIT370 from surface sterilized internal tissue from the fruits that were purchased from a farmer’s market in Rochester, New York
Summary
The plant genus Capsium (Solanaceae) is of interest given its use in a number of herbal remedies by indigenous inhabitants throughout the world and the ability to synthesize the antibacterial metabolite and the active ingredient capsaicin (8-methyl-Nvanillyl-6-nonenamide) which is predominantly stored in the fruit of the plant [1, 2]. This resulted in the isolation and identification of Bacillus safensis RIT372 and Pseudomonas oryzihabitans RIT370 from surface sterilized internal tissue from the fruits that were purchased from a farmer’s market in Rochester, New York.
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