Abstract
ABSTRACTBifidobacterium longum, a Gram-positive rod-shaped anaerobic bacterium, inhabits the human gastrointestinal tract and contributes significantly to oligosaccharide production, amino acid metabolism, and protection against intestinal inflammation. Here, we report the whole-genome sequence of B. longum, which was isolated from the gastrointestinal tract of a healthy Indian adult.
Highlights
Different species of bifidobacteria play important roles in the homeostasis of the gut microbiome
B. longum strain Indica was isolated from the fecal samples of a healthy Indian adult
The fresh fecal sample was immediately resuspended in prereduced phosphatebuffered saline, serially diluted, and plated onto a tryptic soy agar (Difco) plate supplemented with sterile defibrinated sheep blood 5%
Summary
Different species of bifidobacteria play important roles in the homeostasis of the gut microbiome. B. longum strain Indica was isolated from the fecal samples of a healthy Indian adult. The fresh fecal sample was immediately resuspended in prereduced phosphatebuffered saline, serially diluted, and plated onto a tryptic soy agar (Difco) plate supplemented with sterile defibrinated sheep blood 5% (vol/vol). The plates were incubated for 24 h at 37°C in an anaerobic workstation (Whitley DG250) filled with a mixture of anaerobic gases (80% N2, 10% CO2, 10% H2).
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