Abstract
ABSTRACTReported here are the genome sequences of three Vibrio coralliilyticus isolates RE87, AIC-7, and 080116A. Each strain was isolated in association with oyster larvae in commercial aquaculture systems. These draft genomes will be useful for further studies in understanding the genomic features contributing to V. coralliilyticus pathogenicity.
Highlights
Vibrio coralliilyticus is a known pathogen of corals [1] and shellfish, including Pacific oyster (Crassostrea gigas) larvae [2,3,4,5]
The assembly for strain RE87 had a genome size of 5.59 Mbp (45.8% GC), 49 contigs (N50 ϭ 335,391 bp), and a maximum contig size of 1,074,445 bp; the assembly for strain AIC-7 resulted in a genome size of 5.95 Mbp (45.3% GC), 65 contigs (N50 ϭ 274,060 bp), and a maximum contig size of 1,201,114 bp; and the assembly for strain 080116A had a genome size of 5.63 Mb (45.7% GC), 107 contigs (N50 ϭ 275,484 bp), and a maximum contig size of 1,249,611 bp
There were 5,145, 5,542, and 5,205 coding sequences detected for strains RE87, AIC-7, and 080116A, respectively
Summary
Vibrio coralliilyticus is a known pathogen of corals [1] and shellfish, including Pacific oyster (Crassostrea gigas) larvae [2,3,4,5]. DNA from overnight cultures was isolated and the Nextera XT kit (Illumina) was used to prepare libraries for sequencing on a MiSeq Illumina platform at the Center for Genome Research and Biocomputing at Oregon State University. Each genome library was sequenced with 250-bp paired-end reads at an estimated minimum coverage of 65-fold.
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