Observation of pancreatic microcirculation in hyperlipidemic acute pancreatitis of hamster model

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Objective To observe the effect of hyperlipidemia on pancreatic microcirculation in an intact hyperlipidemic acute pancreatitis animal moldel. Methods Among 30 healthy hamsters enrolled, 20 were fed normally while, the other 10 were given high fat feed for four weeks.Then 10 animals given normal feed with normal serum lipids and no ultrasonic changes in the organs including liver, biliary tract and pancreas, were randomly(random number) selected and divided into normal control group (C group) and acute pancreatitis group (AP group) averagely.Five animals given high fat feed with 3 times serum normal triglyceride level and no ultrasonic changes in the organs including liver, biliary tract and pancreas, were randomly selected as hyperlipidemic acute pancreatitis group(HLAP). The animals in C group were undergone fake surgery.While the animals in other two groups were carried out surgery based on Schmidt method and were injected 3.5% taurocholic acid sodium into pancreatic duct with a infusion pump. Acridine orange (2 mL/kg) was injected in the inferior vena cava 6 hours later, then laser confocal microscope fluorescence imaging system were utilized to dynamically observe pancreatic microcirculation in living animal models and the variables including average microvascular diameter (MVD), functional capillary density (FCD), microvessel flow velocity (MFV) and leukocyte adhesion were recorded. Pancreatic tissue were collected and cut slices for pathological grading. Result When compared with AP group and C group, pancreas microcirculation function index MVD(μm), FCD(piece/mm2), MFV(μm/s) in the HLAP group decreased, while leukocyte adhesion (piece/mm2) increased significantly[MVD: HLAP group 2.40±0.26 vs.AP group 5.54±0.43, C group 7.56±0.42 (P<0.05); FCD: HLAP group 4.20±0.84 vs.AP group 7.56±1.14, C group 11.40±1.14(P<0.05); MFV: HLAP group 58.80±9.63 vs.AP group 131.00±12.94, C group 224.40±15.63 (P<0.05); leukocyte adhesion: HLAP group 343.60±13.86 vs.AP group 114.00±8.03, C group 18.80±2.28; (P<0.05)]. The pancreatic pathology score was higher in HLAP group [HLAP group 10.00±1.59 vs.AP group 6.60±1.14, C group 1.00±0.71(P<0.05)]. Conclusion Hyperlipidemia may aggravate the impair ment of pancreatic microcirculation and promote microcirculation dysfunction. Key words: Pancreatitis; Hyperlipidemia; Hamster; Animal model; Microcirculation system of pancreas