Ion currents and gap junction coupling in isolated radial glial cells

Abstract

Event Abstract Back to Event Ion currents and gap junction coupling in isolated radial glial cells Márta Jelitai1*, Károly Markó1, Nóra Hádinger1 and Emília Madarász1 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary According to the accepted view, cell-physiological properties of stem and progenitor cells contribute to the maintenance of a non-differentiated status even in a highly differentiated environment, but help to respond to specific differentiation inducing signals. The cellular “excitability” and responsiveness to environmental stimuli are strongly influenced by the local changes in the intracellular distribution of ions. Changes in the expression and in the functional properties of ion channels play deterministic role in neuronal commitment and neuronal differentiation. In the current work, the characteristics of selectively isolated radial glial cells from embryonic or adult nerve tissue- were studied using dye spreading and patch-clamp techniques. Radial glial cells are less gap junction coupled than the neural stem cells and the rate of gap junction coupling changes dynamically. According to the level of gap junction coupling radial glial cells display passive conductance. Delayed-rectifying outward potassium current was expressed almost in all isolated radial glial cell, however the pharmacological properties of the current changed significantly with development. In cells with neuronal morphology voltage dependent sodium current was recorded. The characterization of voltage-dependent ion currents and their developmental appearance may reveal some regulatory roles of ion channel functions in the maintenance of the progenitor stage. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Cellular neuroscience Citation: Jelitai M, Markó K, Hádinger N and Madarász E (2010). Ion currents and gap junction coupling in isolated radial glial cells. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00071 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Apr 2010; Published Online: 21 Apr 2010. * Correspondence: Márta Jelitai, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, jelitai@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Márta Jelitai Károly Markó Nóra Hádinger Emília Madarász Google Márta Jelitai Károly Markó Nóra Hádinger Emília Madarász Google Scholar Márta Jelitai Károly Markó Nóra Hádinger Emília Madarász PubMed Márta Jelitai Károly Markó Nóra Hádinger Emília Madarász Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.