Isolation and characterization of radial glia-like neural stem cells from different regions of adult mouse brain

Abstract

Event Abstract Back to Event Isolation and characterization of radial glia-like neural stem cells from different regions of adult mouse brain T. Kőhidi1, K. Markó1*, N. Hádinger1 and E. Madarász1 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary Previously we have found that preferential adhesion to a novel synthetic peptide, AK-cyclo[RGDfC] provides a unique method for the selective isolation of neural stem cells from embryonic mouse brain. These radial glia-like neural stem/progenitor cells proliferate without any differentiation on the peptide-covered surfaces under serum-free culture conditions in the presence of EGF as the only growth factor supplement. They can give rise to all the major neural cell types, as neurons, astrocytes and oligodendrocytes if the appropriate inductive signals are applied. Recently we have found that a similar method enables the isolation of radial glia-like progenitors from the adult mouse brain. Besides the well-known neurogenic regions of the adult brain (as the subependymal zone of the lateral ventricles and the hippocampus), we were able to select multipotential cells from distinct regions of the brain (as the neocortex and the midbrain) as well. We have established one-cell derived clones from the populations derived from the different brain regions and compared their gene expression profile, regional characteristics and their differentiation potential. Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Kőhidi T, Markó K, Hádinger N and Madarász E (2011). Isolation and characterization of radial glia-like neural stem cells from different regions of adult mouse brain. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00161 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. K. Markó, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, marko.karoly@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers T. Kőhidi K. Markó N. Hádinger E. Madarász Google T. Kőhidi K. Markó N. Hádinger E. Madarász Google Scholar T. Kőhidi K. Markó N. Hádinger E. Madarász PubMed T. Kőhidi K. Markó N. Hádinger E. Madarász Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.