The atypical cadherin Cdh13 and its role in migration and differentiation events during mouse cortical development

Abstract

Event Abstract Back to Event The atypical cadherin Cdh13 and its role in migration and differentiation events during mouse cortical development Kinga Bercsényi1*, Kornél Géczi1, Mátyás Mayer1, Márta Jelitai1, Emília Madrász1, Gábor Szabó1 and Zsolt Lele1 1 Hungarian Academy of Science, Institute of Experimental Medicine, Hungary Cadherin-13 (Cdh13, H-cadherin or T-cadherin) is a unique, GPI-anchored member of the cadherin superfamily. Its role during normal angiogenesis and tumor neovascularization has been extensively studied, but so far no data has been published about its function during CNS development. Here we describe the saptio-temporal pattern of Cdh13 expression during mouse brain development and present in vitro and in vivo data which reveal its function during cortical development. In E13 mouse brain cdh13 mRNA is present only in the cortical plate of the pallium. Later (P0 and adult) expression appears in the basal ganglia, olfactory bulb, nucl. reticularis of the thalamus and cerebellum. In addition the cortical and hippocampal expression becomes restricted to specific neuron groups identified as layer V corticospinal motoneurons and a subset of randomly distributed GABAergic interneurons. Cell culture studies using the embryonic cortical 4EC line reveal the presence of Cdh13 protein within the nucleus and cytosol of undifferentiated cells. Upon differentiation using retinoic acid Cdh12 increases significantly and more importantly it is relocated to the cell membrane indicating important role during neural differentiation. Overexpression of Cdh13 in the telencephalic ventricular zone (where it is normally not expressed) at E14 using in utero elevtroporation had significant effect on both migration and differentiation of the future layer II-III pyramidal neurons. Their radial migration through the SVZ-IZ layers of the cortex appears to be delayed significantly. Surprisingly, these precursors differentiate within the SVZ-IZ, a phenomenon that never occurs in control animals. Moreover a significant part of these neurons differentiate prematurally into GFAP- and RC2 positive cells with long extensions and end-feet typical of radial glia. Currently we are describing this phenomenon in more detail in an effort to pinpoint the role Cdh 13 plays during cortical development. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Development Citation: Bercsényi K, Géczi K, Mayer M, Jelitai M, Madrász E, Szabó G and Lele Z (2010). The atypical cadherin Cdh13 and its role in migration and differentiation events during mouse cortical development. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00014 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 16 Apr 2010; Published Online: 16 Apr 2010. * Correspondence: Kinga Bercsényi, Hungarian Academy of Science, Institute of Experimental Medicine, Budapest, Hungary, pocokom@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Kinga Bercsényi Kornél Géczi Mátyás Mayer Márta Jelitai Emília Madrász Gábor Szabó Zsolt Lele Google Kinga Bercsényi Kornél Géczi Mátyás Mayer Márta Jelitai Emília Madrász Gábor Szabó Zsolt Lele Google Scholar Kinga Bercsényi Kornél Géczi Mátyás Mayer Márta Jelitai Emília Madrász Gábor Szabó Zsolt Lele PubMed Kinga Bercsényi Kornél Géczi Mátyás Mayer Márta Jelitai Emília Madrász Gábor Szabó Zsolt Lele Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.