Estradiol replacement evokes a wide range of transcriptional changes in the frontal cerebral cortex of middle-aged, ovariectomized rats

Abstract

Event Abstract Back to Event Estradiol replacement evokes a wide range of transcriptional changes in the frontal cerebral cortex of middle-aged, ovariectomized rats M. Sárvári1*, I. Kalló2, E. Hrabovszky1, N. Solymosi3, K. Tóth4, I. Likó5, B. Molnár6, K. Tihanyi5 and Z. Liposits1 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary 2 Pázmány Péter Catholic University, Faculty of Information Technology, Hungary 3 Eötvös Loránd University, Department of the Physics of Complex Systems, Hungary 4 Semmelweis University, Second Department of Medicine, Hungary 5 Gedeon Richter Plc., Hungary 6 Hungarian Academy of Sciences, Molecular Medicine Research Unit, Hungary Estradiol (E2) plays an important role in the maintenance of cortical functions. Around menopause, the incidence of cognitive disturbances and mood fluctuations increases. The symptoms can be counterbalanced by hormone replacement supporting the idea that estrogens help to maintain the function of the frontal cortex. We used middle-aged, ovariectomized rats and E2 replacement for 4 weeks to dissect the role of the genomic effects of E2 in the maintenance of the function of the frontal cortex. The expression level of 28,000 transcripts was determined by oligonucleotide microarray resulting in the identification of ten E2-regulated functions: neurotransmission (Adora2a, Drd1a, Drd2, Cartpt, Nts, Tac1), signal transduction (Igf2, Igfbp2, Igfbp6), transcription (Etv4, Irf7), transport (Abca1, Hba-a2), extracellular matrix (Col1a2), immunity (C3, CD74), metabolism (Acer2, Osbpl3), miscellaneous and unknown functions. After selection of 39 genes for validation, 74 percent of the changes were confirmed by quantitative real-time PCR. The genomic effects of E2 alter elements of dopaminergic and peptidergic neurotransmission, adenosine and IGF signaling and immune surveillance. These results suggest that the genomic effects of estrogens may play a significant role in the maintenance of cortical functions during hormone replacement in postmenopausal women. Acknowledgements Supported by the Hungarian Scientific Research Fund (OTKA K69127 and T73002) and the EU Seventh Framework Programme (FP7/2007-2013). Keywords: Neuroendocrinology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neuroendocrinology Citation: Sárvári M, Kalló I, Hrabovszky E, Solymosi N, Tóth K, Likó I, Molnár B, Tihanyi K and Liposits Z (2011). Estradiol replacement evokes a wide range of transcriptional changes in the frontal cerebral cortex of middle-aged, ovariectomized rats. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00028 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. M. Sárvári, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, sarvari@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers M. Sárvári I. Kalló E. Hrabovszky N. Solymosi K. Tóth I. Likó B. Molnár K. Tihanyi Z. Liposits Google M. Sárvári I. Kalló E. Hrabovszky N. Solymosi K. Tóth I. Likó B. Molnár K. Tihanyi Z. Liposits Google Scholar M. Sárvári I. Kalló E. Hrabovszky N. Solymosi K. Tóth I. Likó B. Molnár K. Tihanyi Z. Liposits PubMed M. Sárvári I. Kalló E. Hrabovszky N. Solymosi K. Tóth I. Likó B. Molnár K. Tihanyi Z. Liposits Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.