Cholinergic control of GABA release by carbachol at perisomatic inhibitory synapses in the CA3 region of mouse hippocampus

Event Abstract Back to Event Convergence of parvalbumin and type 1 cannabinoid receptor positive perisomatic inputs onto hippocampal pyramidal cells V. T. Takács1*, T. F. Freund1 and A. I. Gulyás1 1 Hungarian Academy of Sciences, Dept. of Cellular and Network Neurobiology, Institute of Experimental Medicine, Hungary The soma and proximal dendrites of cortical pyramidal cells are innervated by two functionally distinct types of perisomatic interneurons: the parvalbumin (PV)- and the cholecystokinin-containing, type 1 cannabinoid receptor (CB1) positive basket cells. Here we estimated the proportion of PV- and CB1-positive basket cell inputs in mice by reconstructing parts of hippocampal CA1 pyramidal cell somata and the contacting boutons from serial electron microscopic sections using a new double staining method that gives distinguishable signal at the electron microscopic level. All but 1% of the somatic boutons were labeled by one of the markers. The number of PV- and CB1-positive boutons/soma was ~35 and ~23, respectively. Next, we have measured some of the ultrastructural parameters of 3D reconstructed immunogold-labeled PV- and CB1 positive boutons. The volume occupied by mitochondria was significantly larger in PV-positive boutons. Both types formed more than one synapses often on the same soma or an additional soma or a dendrite. PV-positive boutons possessed small, macular shaped active zones. Synapses of CB1-positive boutons formed a complex structure with multiple small and large irregular shaped active zones. The total synaptic areas/bouton was significantly larger in the case of CB1-positive boutons. Our results show that the physiological differences of the two basket cell types are mirrored by morphological diversity of their boutons. Acknowledgements Support: NKTH-OTKA CNK 77793, NIH DA11322. Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Takács VT, Freund TF and Gulyás AI (2011). Convergence of parvalbumin and type 1 cannabinoid receptor positive perisomatic inputs onto hippocampal pyramidal cells. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00051 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. V. T Takács, Hungarian Academy of Sciences, Dept. of Cellular and Network Neurobiology, Institute of Experimental Medicine, Budapest, Hungary, viragt@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers V. T Takács T. F Freund A. I Gulyás Google V. T Takács T. F Freund A. I Gulyás Google Scholar V. T Takács T. F Freund A. I Gulyás PubMed V. T Takács T. F Freund A. I Gulyás Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event A unique distribution pattern of the Kv4.2 channels on hippocampal CA1 pyramidal cells is revealed by freeze-fracture replica labeling K. M. Kerti1, A. Lőrincz1 and Z. Nusser1* 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary A-current flowing through Kv4.2 voltage-gated K+ channels plays a critical role in controlling pyramidal cell (PC) excitability. Electrophysiological recordings have demonstrated a 6-fold increase in A-type K+ current density along the somato-dendritic axis of hippocampal CA1 PCs. However, the underlying channel distribution and densities remained unknown. Here we examined the subcellular distribution and densities of Kv4.2 subunits in CA1 PC somata and 11 distinct dendritic compartments using a highly sensitive immunogold method (SDS-digested freeze-fracture replica labeling). Only a moderate (only 50%) increase in Kv4.2 immunogold density was observed along the proximo-distal axis of apical dendrites in the stratum radiatum with a slight decrease in stratum lacunosum-moleculare. This was characteristic to all dendritic compartments, including main apical dendrites, oblique dendrites and dendritic spines. In addition to the somato-dendritic localization, the Kv4.2 subunit was also found in axon terminals at a 4-fold lower density. Our results demonstrate a novel distribution pattern of the Kv4.2 subunit along the axo-somato-dendritic surface of CA1 PCs and suggest that the strong increase in the A-current density cannot be solely explained by a corresponding increase in channel number. Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Kerti KM, Lőrincz A and Nusser Z (2011). A unique distribution pattern of the Kv4.2 channels on hippocampal CA1 pyramidal cells is revealed by freeze-fracture replica labeling. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00153 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. Z. Nusser, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, nusser@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers K. M Kerti A. Lőrincz Z. Nusser Google K. M Kerti A. Lőrincz Z. Nusser Google Scholar K. M Kerti A. Lőrincz Z. Nusser PubMed K. M Kerti A. Lőrincz Z. Nusser Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event Estradiol replacement evokes a wide range of transcriptional changes in the frontal cerebral cortex of middle-aged, ovariectomized rats M. Sárvári1*, I. Kalló2, E. Hrabovszky1, N. Solymosi3, K. Tóth4, I. Likó5, B. Molnár6, K. Tihanyi5 and Z. Liposits1 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary 2 Pázmány Péter Catholic University, Faculty of Information Technology, Hungary 3 Eötvös Loránd University, Department of the Physics of Complex Systems, Hungary 4 Semmelweis University, Second Department of Medicine, Hungary 5 Gedeon Richter Plc., Hungary 6 Hungarian Academy of Sciences, Molecular Medicine Research Unit, Hungary Estradiol (E2) plays an important role in the maintenance of cortical functions. Around menopause, the incidence of cognitive disturbances and mood fluctuations increases. The symptoms can be counterbalanced by hormone replacement supporting the idea that estrogens help to maintain the function of the frontal cortex. We used middle-aged, ovariectomized rats and E2 replacement for 4 weeks to dissect the role of the genomic effects of E2 in the maintenance of the function of the frontal cortex. The expression level of 28,000 transcripts was determined by oligonucleotide microarray resulting in the identification of ten E2-regulated functions: neurotransmission (Adora2a, Drd1a, Drd2, Cartpt, Nts, Tac1), signal transduction (Igf2, Igfbp2, Igfbp6), transcription (Etv4, Irf7), transport (Abca1, Hba-a2), extracellular matrix (Col1a2), immunity (C3, CD74), metabolism (Acer2, Osbpl3), miscellaneous and unknown functions. After selection of 39 genes for validation, 74 percent of the changes were confirmed by quantitative real-time PCR. The genomic effects of E2 alter elements of dopaminergic and peptidergic neurotransmission, adenosine and IGF signaling and immune surveillance. These results suggest that the genomic effects of estrogens may play a significant role in the maintenance of cortical functions during hormone replacement in postmenopausal women. Acknowledgements Supported by the Hungarian Scientific Research Fund (OTKA K69127 and T73002) and the EU Seventh Framework Programme (FP7/2007-2013). Keywords: Neuroendocrinology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neuroendocrinology Citation: Sárvári M, Kalló I, Hrabovszky E, Solymosi N, Tóth K, Likó I, Molnár B, Tihanyi K and Liposits Z (2011). Estradiol replacement evokes a wide range of transcriptional changes in the frontal cerebral cortex of middle-aged, ovariectomized rats. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00028 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. M. Sárvári, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, sarvari@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers M. Sárvári I. Kalló E. Hrabovszky N. Solymosi K. Tóth I. Likó B. Molnár K. Tihanyi Z. Liposits Google M. Sárvári I. Kalló E. Hrabovszky N. Solymosi K. Tóth I. Likó B. Molnár K. Tihanyi Z. Liposits Google Scholar M. Sárvári I. Kalló E. Hrabovszky N. Solymosi K. Tóth I. Likó B. Molnár K. Tihanyi Z. Liposits PubMed M. Sárvári I. Kalló E. Hrabovszky N. Solymosi K. Tóth I. Likó B. Molnár K. Tihanyi Z. Liposits Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event Postsynaptic Group II Metabotropic Glutamate Receptor activation in Dentate Gyrus Granule Cells J. Brunner1*, T. Andrási1, S. Van-Weert1, F. K. Borgmann2, S. Jessberger2 and J. Szabadics1 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary 2 Swiss Federal Institute of Technology, Institute of Cell Biology, Switzerland Dentate gyrus granule cells (DGGCs) express group II metabotropic glutamate receptors (mGluR2/3) both on their axonal and somatodendritic membranes. Activation of axonal mGluR2/3s suppress the synaptic transmission from DGGCs due to down regulation of presynaptic Ca2+ channel activity. However, the function of dendritic mGluR2/3s in DGGCs is not known. First, we showed that bath application of mGluR2/3 selective agonists (DCG IV, APDC) resulted in significant, long-lasting hyperpolarization in DGGCs and this effect persisted in the presence of TTX but it was prevented by selective antagonists (APICA, LY 341495). DCG IV did not have any effect on the axonal membrane potential of DGGCs indicating distinct somatic and axonal mGluR2/3 mediated mechanisms. The hyperpolarizing DCG IV effect was absent in GABAergic and CA3 pyramidal cells. We also showed that the mGluR2/3 activation induced somatodendritic hyperpolarization was mediated by GIRK channels as intracellular GDPβS and bath application of inward-rectifier potassium channel blocker, tertiapin-Q occluded the DCG IV effect. Lastly, focal glutamate release by photolysis of MNI-glutamate in the perisomatic region of DGGCs resulted in mGluR2/3 mediated currents; however, similar current could be evoked in the dendritic region (>15 µm) suggesting that mGluR2/3 are activated by a specific glutamatergic source which innervates the perisomatic region of DGGCs and elicit mGluR2/3 mediated glutamatergic inhibition. Keywords: Neurophysiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neurophysiology Citation: Brunner J, Andrási T, Van-Weert S, Borgmann FK, Jessberger S and Szabadics J (2011). Postsynaptic Group II Metabotropic Glutamate Receptor activation in Dentate Gyrus Granule Cells. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00100 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. J. Brunner, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, brunner.janos@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers J. Brunner T. Andrási S. Van-Weert F. K Borgmann S. Jessberger J. Szabadics Google J. Brunner T. Andrási S. Van-Weert F. K Borgmann S. Jessberger J. Szabadics Google Scholar J. Brunner T. Andrási S. Van-Weert F. K Borgmann S. Jessberger J. Szabadics PubMed J. Brunner T. Andrási S. Van-Weert F. K Borgmann S. Jessberger J. Szabadics Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event Virally mediated functional suppression of GABA-A receptors in the rodent thalamus Z. Rovó1*, F. Mátyás1, P. Barthó1, A. Slézia1 and L. Acsády1 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary GABAergic inhibition is essential for the normal function of thalamocortical networks. In addition beside the phasic inhibition mediated by synaptic GABA-A receptors, extrasynaptic GABA-A receptors also exert powerful tonic inhibition on thalamocortical cells. The nucleus specific role of synaptic inhibition is difficult to assess using the traditional pharmacological or KO methods due to the limited spatial and temporal control of their effects. Thus, in this study, we used stereotaxic injection of viral particles to focally knock down synaptic GABA-A receptors. All synaptic GABA-A receptors in the thalamus contain g2 subunit. Injection of AAV-Cre viral particles into the thalamus of g2 floxed mice results in the focal removal of the g2 gene. Successful removal of the g2 gene was identified by immunocytochemistry for g2 protein. Immunoreactivity of the other subunits of synaptic GABA-A receptors (a1, b2) as well as extrasynaptic receptors (alpha4) remained unchanged. Preliminary juxtacellular recording in anaesthetized mice demonstrated that it is possible to record, label and identify virally infected thalamocortical cells, which lack functional synaptic GABA-A receptors and to differentiate them from non-infected cells with intact inhibition. Our data demonstrate that the present method is an excellent tool to study the nucleus specific role of synaptic GABA-A receptor in cellular and network activity. Keywords: Neurophysiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neurophysiology Citation: Rovó Z, Mátyás F, Barthó P, Slézia A and Acsády L (2011). Virally mediated functional suppression of GABA-A receptors in the rodent thalamus. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00026 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. Z. Rovó, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, rovozita@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Z. Rovó F. Mátyás P. Barthó A. Slézia L. Acsády Google Z. Rovó F. Mátyás P. Barthó A. Slézia L. Acsády Google Scholar Z. Rovó F. Mátyás P. Barthó A. Slézia L. Acsády PubMed Z. Rovó F. Mátyás P. Barthó A. Slézia L. Acsády Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event Developmental expression of type 2 deiodinase in the chicken hypothalamus P. Mohácsik1, T. Füzesi1, E. Hadadi1, E. Szabó1, M. Doleschall1, P. Egri1, Z. Liposits1, C. Fekete1 and B. Gereben1* 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary Thyroid hormone is an essential factor of brain development and function. Thyroxine is a pro-hormone that needs to be converted to 3,5,3’-triiodothyronine (T3) to be able to bind the thyroid hormone receptors. Type 2 deiodinase (D2) is the key enzyme that generates T3 in the brain. D2 is abundantly expressed in hypothalamic tanycytes that are special glial cells lining the floor and walls of the third ventricles. In chicken, adenohypophysis starts promoting thyroidal secretion at embryonic day (E) 11.5, while thyroid hormones start exerting their negative feedback effect on the TSH production only at E19. It can be hypothesized that a special pattern of D2-mediated T3 generation in developing hypothalamus could be required for the development of T3 dependent negative feedback. Using in situ hybridization, we detected D2 mRNA in tanycytes in the floor and walls of the third ventricle at E13 in the chicken mediobasal hypothalamus. At this stage, the mRNA of the Nkx-2.1 transcription factor could be also detected in tanycytes while it was absent from the perivascular space where D2 expressing astrocytes are present. Promoter studies in U87 glioma cells proved that Nkx-2.1 can induce transcription of the cdio2 gene. In conclusion, the appearance of Nkx-2.1 mediated D2 expression in chicken tanycytes well before the onset of feedback suggests that D2 expression in these cells could be required, but not sufficient to initiate T3 dependent negative feedback in the hypothalamus. Keywords: Neuroendocrinology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neuroendocrinology Citation: Mohácsik P, Füzesi T, Hadadi E, Szabó E, Doleschall M, Egri P, Liposits Z, Fekete C and Gereben B (2011). Developmental expression of type 2 deiodinase in the chicken hypothalamus. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00177 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. B. Gereben, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, gereben@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers P. Mohácsik T. Füzesi E. Hadadi E. Szabó M. Doleschall P. Egri Z. Liposits C. Fekete B. Gereben Google P. Mohácsik T. Füzesi E. Hadadi E. Szabó M. Doleschall P. Egri Z. Liposits C. Fekete B. Gereben Google Scholar P. Mohácsik T. Füzesi E. Hadadi E. Szabó M. Doleschall P. Egri Z. Liposits C. Fekete B. Gereben PubMed P. Mohácsik T. Füzesi E. Hadadi E. Szabó M. Doleschall P. Egri Z. Liposits C. Fekete B. Gereben Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event Glutamatergic input from specific sources influences the nucleus accumbens-ventral pallidum information flow E. Papp1*, Z. Borhegyi2, R. Tomioka2, K. S. Rockland3, I. Mody4 and T. F. Freund1 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary 2 Medical University of Vienna, Center for Brain Research, Austria 3 MIT, Picower Inst. Learning and Memory, RIKEN-MIT Center for Neural Circuit Genetics, United States 4 University of California, Departments of Neurology and Physiology, United States The nucleus accumbens (NAc) is positioned to integrate signals originating from limbic and cortical areas and to modulate reward-related motor output of various goal-directed behaviours. The major target of the NAc GABAergic output neurons is the ventral pallidum (VP). VP is part of the reward circuit, and controls the ascending mesolimbic dopamine system, as well as the motor output structures and the brainstem. The excitatory inputs governing this system converge in the NAc from the prefrontal cortex (PFC), ventral hippocampus (vHC), paraventricular thalamic nucleus (PVT) and basolateral nucleus of the amygdala (BLA). It is unclear which if any of these afferents innervate the medium spiny neurons of the NAc, that project to the VP. To identify the source of glutamatergic afferents that innervate neurons projecting to the VP, a dual-labelling method was used: Phaseolus vulgaris leucoagglutinin for anterograde and EGFP-encoded adenovirus for retrograde tract-tracing. Within the NAc, anterogradely labelled BLA terminals formed asymmetric synapses on dendritic spines that belonged to medium spiny neurons retrogradely labelled from the VP. PVT terminals also formed synapses on dendritic spines of NAc neurons projecting to the VP. However, dendrites and dendritic spines retrogradely labelled from VP received no direct synaptic contacts from afferents originating from mPFC and vHC in the present material, despite the large number of fibres labelled by the anterograde tracer injections. These findings represent the first experimental evidence for a selective glutamatergic innervation of NAc neurons projecting to the VP. Keywords: Neurophysiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neurophysiology Citation: Papp E, Borhegyi Z, Tomioka R, Rockland KS, Mody I and Freund TF (2011). Glutamatergic input from specific sources influences the nucleus accumbens-ventral pallidum information flow. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00015 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. E. Papp, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, papp.edit@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers E. Papp Z. Borhegyi R. Tomioka K. S Rockland I. Mody T. F Freund Google E. Papp Z. Borhegyi R. Tomioka K. S Rockland I. Mody T. F Freund Google Scholar E. Papp Z. Borhegyi R. Tomioka K. S Rockland I. Mody T. F Freund PubMed E. Papp Z. Borhegyi R. Tomioka K. S Rockland I. Mody T. F Freund Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event Cocaine and amphetamine regulated transcript, type-2 vesicular glutamate transporter and calbindin co-expression define spiny stellate cells in layer IV of the granular cerebral cortex C. S. Molnár1, M. Sárvári1, I. Kalló2, Z. Liposits1, 2 and E. Hrabovszky1* 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary 2 Pázmány Péter Catholic University, Department of Neuroscience, Hungary Microarray experiments from our laboratory identified cocaine and amphetamine regulated transcript (CART) as an estrogen-regulated cortical gene. The present studies addressed the anatomical location and functional phenotype of cortical CART neurons using immunohistochemical (IHC) and in situ hybridization (ISH) strategies. IHC and ISH studies localized CART expressing neurons in layer IV throughout the granular cortex of the rat. CART neurons were found to be distinct from GABAergic interneurons expressing glutamic acid decarboxylase-65 mRNA. Out of the Ca-binding proteins calretinin, parvalbumin and calbindin, CART neurons only expressed low levels of calbindin. ISH studies established that the distribution of CART/calbindin neurons was identical with that of VGLUT2 mRNA expressing cells in layer IV. Morphological studies with IHC determined that CART/VGLUT2/calbindin neurons correspond to spiny stellate cells known to establish local efferent cortical connections. Future studies will use electrophysiological tools to address the effects of CART on cortical neuronal functioning and molecular approaches to study the regulation of cortical CART expression under various physiological and pathological conditions. Acknowledgements This work was supported by grants from the Hungarian Scientific Research Fund (OTKA K69127 T73002 and K83710), the Hungarian Health Research Council Fund (ETT 122/2009) and the European Community's Seventh Framework Programme (FP7/2007-2013; grant agreement n° 245009). Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Molnár CS, Sárvári M, Kalló I, Liposits Z and Hrabovszky E (2011). Cocaine and amphetamine regulated transcript, type-2 vesicular glutamate transporter and calbindin co-expression define spiny stellate cells in layer IV of the granular cerebral cortex. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00001 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. E. Hrabovszky, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, hrabovszky.erik@koki.mta.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers C. S Molnár M. Sárvári I. Kalló Z. Liposits E. Hrabovszky Google C. S Molnár M. Sárvári I. Kalló Z. Liposits E. Hrabovszky Google Scholar C. S Molnár M. Sárvári I. Kalló Z. Liposits E. Hrabovszky PubMed C. S Molnár M. Sárvári I. Kalló Z. Liposits E. Hrabovszky Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event Glutamatergic and GABAergic innervation of GnRH neurons in the human hypothalamus E. Hrabovszky1*, C. S. Molnár1, I. Kallo2, B. Borsay3, L. Sarkadi3, L. Herczeg3, M. Watanabe4 and Z. Liposits1, 2 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary 2 Pázmány Péter Catholic University, Department of Neuroscience, Hungary 3 University of Debrecen, Department of Forensic Medicine, Hungary 4 Hokkaido University School of Medicine, Department of Anatomy, Japan Neurosecretory projections of gonadotropin-releasing hormone (GnRH) neurons to the hypophysial portal capillaries represent the final common output way of the hypothalamus in the neuroendocrine control of reproduction. GnRH neurons receive afferent input from a variety of neurotransmitter systems which are only partly characterized in the human. In the present study we used immunohistochemical approaches to address the contribution of the amino acid neurotransmitters GABA and glutamate to the afferent regulation of human GnRH neurons. According to previous findings in rodents where GABA represents the main neurotransmitter in the afferent control of GnRH neurons, we found that vesicular GABA transporter-immunoreactive GABAergic axons abundantly innervated the cell bodies and the dendrites of human GnRH neurons. While in rats, only glutamatergic axons of the VGLUT2 phenotype innervate GnRH neurons, in human samples we observed both VGLUT1- and VGLUT2 immunoreactive afferent contacts on GnRH neurons, with a preferential accumulation on GnRH dendrites. These morphological observations implicate the classic amino acid neurotransmitters GABA and glutamate in the afferent regulation of human GnRH neurons. As in rodents, GABA-ergic and glutamatergic afferents may have important contributions to metabolic-, sex steroid-, circadian- and stress signaling to the reproductive axis. Acknowledgements Supported by: OTKA K69127, T73002 and K83710; ETT 122/2009; FP7/2007-2013 (n° 245009) Keywords: Neuroendocrinology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neuroendocrinology Citation: Hrabovszky E, Molnár CS, Kallo I, Borsay B, Sarkadi L, Herczeg L, Watanabe M and Liposits Z (2011). Glutamatergic and GABAergic innervation of GnRH neurons in the human hypothalamus. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00141 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. E. Hrabovszky, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, hrabovszky.erik@koki.mta.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers E. Hrabovszky C. S Molnár I. Kallo B. Borsay L. Sarkadi L. Herczeg M. Watanabe Z. Liposits Google E. Hrabovszky C. S Molnár I. Kallo B. Borsay L. Sarkadi L. Herczeg M. Watanabe Z. Liposits Google Scholar E. Hrabovszky C. S Molnár I. Kallo B. Borsay L. Sarkadi L. Herczeg M. Watanabe Z. Liposits PubMed E. Hrabovszky C. S Molnár I. Kallo B. Borsay L. Sarkadi L. Herczeg M. Watanabe Z. Liposits Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event Retrograde Endocannabinoid Signaling Reduces Firing and GABA-ergic Synaptic Transmission to Gonadotropin-Releasing Hormone Neurons I. Farkas1*, I. Kalló1, L. Deli1, B. Vida1, E. Hrabovszky1, C. Fekete1, S. M. Moenter2, M. Watanabe3 and Z. Liposits1 1 Institute of Experimental Medicine, Hungarian Academy of Sciences, Department of Endocrine Neurobiology, Hungary 2 University of Virginia, Departments of Medicine and Cell Biology, United States 3 Hokkaido University School of Medicine, Department of Anatomy, Japan Cannabinoids suppress fertility via reducing hypothalamic GnRH output. GABAAR-mediated transmission is a major input to GnRH cells that can be excitatory. We hypothesized that cannabinoids act via inhibiting GABAergic input by a retrograde endocannabinoid signaling mechanism. Loose-patch studies of slices from adult male GnRH-GFP mice showed that bath application of WIN55,212 decreased GnRH neuron firing rate. This action was detectable in presence of kynurenic acid, but disappeared when bicuculline was also present, indicating GABAA-R involvement. In immunocytochemical experiments, CB1-IR axons formed contacts with GnRH neurons and a subset established symmetric synapses characteristic of GABA-ergic neurotransmission. Whole-cell patch-clamp studies revealed that WIN55,212 decreased the frequency of GABAA-R-mediated mPSCs, which was prevented with the AM251, indicating that activation of presynaptic CB1 inhibits GABA release. AM251 alone increased mPSC frequency, providing evidence that endocannabinoids tonically inhibit GABAA-R drive onto GnRH neurons. Increased mPSC frequency was absent when DAG-lipase was blocked intracellularly with THL, showing that tonic inhibition is caused by 2-AG production of GnRH neurons. When CdCl2 was applied to inhibit action potential-evoked calcium influx and endocannabinoid-mediated blockade of spontaneous vesicle fusion was blocked with AM251, GnRH neuron firing increased, revealing an endogenous endocannabinoid brake on GnRH neuron firing. Keywords: Neuroendocrinology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neuroendocrinology Citation: Farkas I, Kalló I, Deli L, Vida B, Hrabovszky E, Fekete C, Moenter SM, Watanabe M and Liposits Z (2011). Retrograde Endocannabinoid Signaling Reduces Firing and GABA-ergic Synaptic Transmission to Gonadotropin-Releasing Hormone Neurons. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00116 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. I. Farkas, Institute of Experimental Medicine, Hungarian Academy of Sciences, Department of Endocrine Neurobiology, Budapest, Hungary, b.farkas@richter.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers I. Farkas I. Kalló L. Deli B. Vida E. Hrabovszky C. Fekete S. M Moenter M. Watanabe Z. Liposits Google I. Farkas I. Kalló L. Deli B. Vida E. Hrabovszky C. Fekete S. M Moenter M. Watanabe Z. Liposits Google Scholar I. Farkas I. Kalló L. Deli B. Vida E. Hrabovszky C. Fekete S. M Moenter M. Watanabe Z. Liposits PubMed I. Farkas I. Kalló L. Deli B. Vida E. Hrabovszky C. Fekete S. M Moenter M. Watanabe Z. Liposits Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event Isolation and characterization of radial glia-like neural stem cells from different regions of adult mouse brain T. Kőhidi1, K. Markó1*, N. Hádinger1 and E. Madarász1 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary Previously we have found that preferential adhesion to a novel synthetic peptide, AK-cyclo[RGDfC] provides a unique method for the selective isolation of neural stem cells from embryonic mouse brain. These radial glia-like neural stem/progenitor cells proliferate without any differentiation on the peptide-covered surfaces under serum-free culture conditions in the presence of EGF as the only growth factor supplement. They can give rise to all the major neural cell types, as neurons, astrocytes and oligodendrocytes if the appropriate inductive signals are applied. Recently we have found that a similar method enables the isolation of radial glia-like progenitors from the adult mouse brain. Besides the well-known neurogenic regions of the adult brain (as the subependymal zone of the lateral ventricles and the hippocampus), we were able to select multipotential cells from distinct regions of the brain (as the neocortex and the midbrain) as well. We have established one-cell derived clones from the populations derived from the different brain regions and compared their gene expression profile, regional characteristics and their differentiation potential. Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Kőhidi T, Markó K, Hádinger N and Madarász E (2011). Isolation and characterization of radial glia-like neural stem cells from different regions of adult mouse brain. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00161 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. K. Markó, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, marko.karoly@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers T. Kőhidi K. Markó N. Hádinger E. Madarász Google T. Kőhidi K. Markó N. Hádinger E. Madarász Google Scholar T. Kőhidi K. Markó N. Hádinger E. Madarász PubMed T. Kőhidi K. Markó N. Hádinger E. Madarász Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event Functional imaging at cellular level in hemicochlea preparation from hearing mice. M. Aller1*, R. Fekete1, T. Horváth1, G. Polony1, Á. Fekete1, G. Halmos1, A. Heinrich1, B. Sperlágh1, B. Lendvai1, E. S. Vizi1 and T. Zelles1 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary Hearing loss (HL) is the most frequent sensory deficit. In contrast to conductive forms, there is no effective treatment for sensorineural hearing losses (SNHLs; e.g. noise-induced HL or presbycusis). One of the main reason for the absence of tools to prevent and cure SNHLs is the insufficient knowledge of basic molecular mechanisms of normal and impaired adult hearing. Cells of the organ of Corti and auditory neurons are pimary targets in SNHLs. Majority of SNHLs are developed after the onset of hearing but investigations at cellular level in mice are usually performed before that. Hemicochlea preparation of hearing mice (>P14) has a structure and metabolism that are closer to in vivo conditions than any other preparations, including isolated cells, ex vivo explants or organotypic cultures from immature ears. We have developed a method of fluorescence imaging in acute hemicochlea preparation using various fluorescence indicators. After loading of the preparation with Fura-2/AM, we could measure repeatable Ca2+ transients in individual cells of the organ of Corti evoked by perfusion of ATP. Using dihydroethidium, a superoxide indicator we detected superoxide production in response to neurotoxic levels of Glu in auditory neurons. Disturbed regulation of Ca2+ and ROS are involved in SNHLs. However, precise description of the mechanisms is missing. Our new method offers the appropriate spatial and temporal resolution to explore their function in the damage of the inner ear. Keywords: methods, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Methods Citation: Aller M, Fekete R, Horváth T, Polony G, Fekete Á, Halmos G, Heinrich A, Sperlágh B, Lendvai B, Vizi ES and Zelles T (2011). Functional imaging at cellular level in hemicochlea preparation from hearing mice.. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00080 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. M. Aller, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, allerm@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers M. Aller R. Fekete T. Horváth G. Polony Á. Fekete G. Halmos A. Heinrich B. Sperlágh B. Lendvai E. S Vizi T. Zelles Google M. Aller R. Fekete T. Horváth G. Polony Á. Fekete G. Halmos A. Heinrich B. Sperlágh B. Lendvai E. S Vizi T. Zelles Google Scholar M. Aller R. Fekete T. Horváth G. Polony Á. Fekete G. Halmos A. Heinrich B. Sperlágh B. Lendvai E. S Vizi T. Zelles PubMed M. Aller R. Fekete T. Horváth G. Polony Á. Fekete G. Halmos A. Heinrich B. Sperlágh B. Lendvai E. S Vizi T. Zelles Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Event Abstract Back to Event Type 1 cannabinoid receptor (CB1)-containing axons innervate magnocellular vasopressin-synthesizing neurons in the hypothalamic paraventricular nucleus in mice Z. Bardóczi1, A. Kádár1, M. Watanabe2, Z. Liposits3, C. Fekete1 and I. Kalló1* 1 Institute of Experimental Medicine, Hungarian Academy of Sciences, Department of Endocrine Neurobiology, Hungary 2 Hokkaido University School of Medicine, Department of Anatomy, Japan 3 Pázmány Péter Catholic University, Department of Neuroscience, Hungary The magnocellular vasopressin-synthesizing neurons of the hypothalamic paraventricular nucleus (PVN) play critical role in the regulation of fluid balance and blood pressure. Recently, the retrograde signaling endocannabinoid system has been shown to exert inhibitory effects on magnocellular neurons via type 1 cannabinoid receptor (CB1). To understand the anatomical basis for this regulatory mechanism, we determined whether CB1 is contained in axons innervating vasopressin-immunoreactive (IR) neurons.At the light microscopic level dense CB1-IR axon network was observed in the PVN. The CB1-IR innervation had punctuated appearance and was the densest in the posterior part of the PVN. In the compact part, at the mid level of the nucleus, where the vasopressin neurons reside, the density of the innervation was relatively lower. However, in double-labeled preparations, CB1-IR varicosities were observed in juxtaposition to the majority of the magnocellular vasopressin neurons. At the electron microscopic level, CB1-immunoreactivity was observed in the pre-terminal portion of axons establishing both symmetric and asymmetric synaptic specializations with the perikaryon and dendrites of vasopressin-IR neurons in the PVN. These data demonstrate that CB1 is abundantly present in both the excitatory and inhibitory axons that are in synaptic association with vasopressin-IR neurons indicating that these neurons can regulate their own input through the endocannabinoid system. Acknowledgements This work was supported by OTKA K69127, T73002, K83710; ETT 122/2009; FP7/2007-2013; grant agreement n° 245009. Keywords: Neuroendocrinology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neuroendocrinology Citation: Bardóczi Z, Kádár A, Watanabe M, Liposits Z, Fekete C and Kalló I (2011). Type 1 cannabinoid receptor (CB1)-containing axons innervate magnocellular vasopressin-synthesizing neurons in the hypothalamic paraventricular nucleus in mice. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00091 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. I. Kalló, Institute of Experimental Medicine, Hungarian Academy of Sciences, Department of Endocrine Neurobiology, Budapest, Hungary, kallo@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Z. Bardóczi A. Kádár M. Watanabe Z. Liposits C. Fekete I. Kalló Google Z. Bardóczi A. Kádár M. Watanabe Z. Liposits C. Fekete I. Kalló Google Scholar Z. Bardóczi A. Kádár M. Watanabe Z. Liposits C. Fekete I. Kalló PubMed Z. Bardóczi A. Kádár M. Watanabe Z. Liposits C. Fekete I. Kalló Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.

Interactions between noradrenergic and cholinergic receptor signaling may be important in some forms of learning. To investigate whether noradrenergic and cholinergic receptor interactions regulate forms of synaptic plasticity thought to be involved in memory formation, we examined the effects of concurrent beta-adrenergic and cholinergic receptor activation on the induction of long-term potentiation (LTP) in the hippocampal CA1 region. Low concentrations of the beta-adrenergic receptor agonist isoproterenol (ISO) and the cholinergic receptor agonist carbachol had no effect on the induction of LTP by a brief train of 5 Hz stimulation when applied individually but dramatically facilitated LTP induction when coapplied. Although carbachol did not enhance ISO-induced increases in cAMP, coapplication of ISO and carbachol synergistically activated p42 mitogen-activated protein kinase (p42 MAPK). This suggests that concurrent beta-adrenergic and cholinergic receptor activation enhances LTP induction by activating MAPK and not by additive or synergistic effects on adenylyl cyclase. Consistent with this, blocking MAPK activation with MEK inhibitors suppressed the facilitation of LTP induction produced by concurrent beta-adrenergic and cholinergic receptor activation. Although MEK inhibitors also suppressed the induction of LTP by a stronger 5 Hz stimulation protocol that induced LTP in the absence of ISO and carbachol, they had no effect on LTP induced by high-frequency synaptic stimulation or low-frequency synaptic stimulation paired with postsynaptic depolarization. Our results indicate that MAPK activation has an important, modulatory role in the induction of LTP and suggest that coactivation of noradrenergic and cholinergic receptors regulates LTP induction via convergent effects on MAPK.

Event Abstract Back to Event Thousand-channel electrode system to investigate thalamocortical interactions D. Horváth1*, B. P. Kerekes2, R. Fiáth1, L. Acsády3, P. H. Neves4, G. Karmos1 and I. Ulbert1 1 Hungarian Academy of Sciences, Institute for Psychology, Hungary 2 Pázmány Péter Catholic University, Faculty of Information Technology, Hungary 3 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary 4 Interuniversity Microelectronics Center (IMEC), Belgium A novel two dimensional silicon-based electrode array was developed in the framework of the NeuroProbes EU project. The electrode array is equipped with electronic depth control system in order to select up to 32 active recording sites from the more than two thousand possible electrode channels without moving the array. The array consists of four shanks forming a comb-like structure. Each shank is 8 mm long and contains a total of 513 electrodes separated by 40 micrometer in two rows. The electrodes can be electronically switched to the eight output lines in 2×2 groups like in a tetrode configuration. As a result, any combination of two tetrodes can be selected on each shank. The complete system consists of the electrode array, switching matrix, front-end electronics, conditioning, multiplexing and interface electronics and the control software. The electrode array was tested in acute experiments. Adult rats under ketamine/xylazine anesthesia were used. The electrode array was implanted into the cortex and the underlying thalamus. We were able to record good quality local field potential and multiunit activity with the aid of the system, simultaneously from the the primary somatosensory cortex and from different nuclei of the thalamus. The analysis of the simultaneously recorded cortical and thalamic signals may give us a powerful tool to reveal more information about the intricate relationship of cortical and thalamic activity. Keywords: methods, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Methods Citation: Horváth D, Kerekes BP, Fiáth R, Acsády L, Neves PH, Karmos G and Ulbert I (2011). Thousand-channel electrode system to investigate thalamocortical interactions. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00140 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. D. Horváth, Hungarian Academy of Sciences, Institute for Psychology, Budapest, Hungary, horvath@phys.szote.u-szeged.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers D. Horváth B. P Kerekes R. Fiáth L. Acsády P. H Neves G. Karmos I. Ulbert Google D. Horváth B. P Kerekes R. Fiáth L. Acsády P. H Neves G. Karmos I. Ulbert Google Scholar D. Horváth B. P Kerekes R. Fiáth L. Acsády P. H Neves G. Karmos I. Ulbert PubMed D. Horváth B. P Kerekes R. Fiáth L. Acsády P. H Neves G. Karmos I. Ulbert Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.