A unique distribution pattern of the Kv4.2 channels on hippocampal CA1 pyramidal cells is revealed by freeze-fracture replica labeling

Abstract

Event Abstract Back to Event A unique distribution pattern of the Kv4.2 channels on hippocampal CA1 pyramidal cells is revealed by freeze-fracture replica labeling K. M. Kerti1, A. Lőrincz1 and Z. Nusser1* 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary A-current flowing through Kv4.2 voltage-gated K+ channels plays a critical role in controlling pyramidal cell (PC) excitability. Electrophysiological recordings have demonstrated a 6-fold increase in A-type K+ current density along the somato-dendritic axis of hippocampal CA1 PCs. However, the underlying channel distribution and densities remained unknown. Here we examined the subcellular distribution and densities of Kv4.2 subunits in CA1 PC somata and 11 distinct dendritic compartments using a highly sensitive immunogold method (SDS-digested freeze-fracture replica labeling). Only a moderate (only 50%) increase in Kv4.2 immunogold density was observed along the proximo-distal axis of apical dendrites in the stratum radiatum with a slight decrease in stratum lacunosum-moleculare. This was characteristic to all dendritic compartments, including main apical dendrites, oblique dendrites and dendritic spines. In addition to the somato-dendritic localization, the Kv4.2 subunit was also found in axon terminals at a 4-fold lower density. Our results demonstrate a novel distribution pattern of the Kv4.2 subunit along the axo-somato-dendritic surface of CA1 PCs and suggest that the strong increase in the A-current density cannot be solely explained by a corresponding increase in channel number. Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Kerti KM, Lőrincz A and Nusser Z (2011). A unique distribution pattern of the Kv4.2 channels on hippocampal CA1 pyramidal cells is revealed by freeze-fracture replica labeling. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00153 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. Z. Nusser, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, nusser@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers K. M Kerti A. Lőrincz Z. Nusser Google K. M Kerti A. Lőrincz Z. Nusser Google Scholar K. M Kerti A. Lőrincz Z. Nusser PubMed K. M Kerti A. Lőrincz Z. Nusser Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.