Convergence of parvalbumin and type 1 cannabinoid receptor positive perisomatic inputs onto hippocampal pyramidal cells

Abstract

Event Abstract Back to Event Convergence of parvalbumin and type 1 cannabinoid receptor positive perisomatic inputs onto hippocampal pyramidal cells V. T. Takács1*, T. F. Freund1 and A. I. Gulyás1 1 Hungarian Academy of Sciences, Dept. of Cellular and Network Neurobiology, Institute of Experimental Medicine, Hungary The soma and proximal dendrites of cortical pyramidal cells are innervated by two functionally distinct types of perisomatic interneurons: the parvalbumin (PV)- and the cholecystokinin-containing, type 1 cannabinoid receptor (CB1) positive basket cells. Here we estimated the proportion of PV- and CB1-positive basket cell inputs in mice by reconstructing parts of hippocampal CA1 pyramidal cell somata and the contacting boutons from serial electron microscopic sections using a new double staining method that gives distinguishable signal at the electron microscopic level. All but 1% of the somatic boutons were labeled by one of the markers. The number of PV- and CB1-positive boutons/soma was ~35 and ~23, respectively. Next, we have measured some of the ultrastructural parameters of 3D reconstructed immunogold-labeled PV- and CB1 positive boutons. The volume occupied by mitochondria was significantly larger in PV-positive boutons. Both types formed more than one synapses often on the same soma or an additional soma or a dendrite. PV-positive boutons possessed small, macular shaped active zones. Synapses of CB1-positive boutons formed a complex structure with multiple small and large irregular shaped active zones. The total synaptic areas/bouton was significantly larger in the case of CB1-positive boutons. Our results show that the physiological differences of the two basket cell types are mirrored by morphological diversity of their boutons. Acknowledgements Support: NKTH-OTKA CNK 77793, NIH DA11322. Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Takács VT, Freund TF and Gulyás AI (2011). Convergence of parvalbumin and type 1 cannabinoid receptor positive perisomatic inputs onto hippocampal pyramidal cells. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00051 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. V. T Takács, Hungarian Academy of Sciences, Dept. of Cellular and Network Neurobiology, Institute of Experimental Medicine, Budapest, Hungary, viragt@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers V. T Takács T. F Freund A. I Gulyás Google V. T Takács T. F Freund A. I Gulyás Google Scholar V. T Takács T. F Freund A. I Gulyás PubMed V. T Takács T. F Freund A. I Gulyás Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.