Abstract
Microbial diversity in geothermal waters of the Unkeshwar hot springs in Maharashtra, India, was studied using 16S rRNA amplicon metagenomic sequencing. Taxonomic analysis revealed the presence of Bacteroidetes, Proteobacteria, Cyanobacteria, Actinobacteria, Archeae, and OD1 phyla. Metabolic function prediction analysis indicated a battery of biological information systems indicating rich and novel microbial diversity, with potential biotechnological applications in this niche.
Highlights
Deccan basaltic geothermal hot springs are rich in sulfur and yet are unexploited for microbial ecology [1]
DNA was enriched by the Multiple Annealing and LoopBased Amplification Cycles (MALBAC) protocol [2] and amplified by using primers spanning the V3 to V4 region of the 16S rRNA gene [3]
Chimeras were removed using the program UCHIME, and all nonchimeric sequences were taken for picking operational taxonomic units (OTUs) using the program Uclust, with a threshold of 97% similarity [5, 6]
Summary
Deccan basaltic geothermal hot springs are rich in sulfur and yet are unexploited for microbial ecology [1]. Replicate water samples were collected during December 2012 in sterile containers, filtered through 0.22-m-pore-size filters (Merck Millipore, India), and DNA extraction was performed using the RNA PowerSoil total RNA isolation kit (Mo Bio Laboratories, Inc., Carlsbad, CA, USA), according to the manufacturer’s protocol. The quality parameters for the obtained sequences were checked using the FASTQ quality filter (Phred quality [Q] Ͻ20).
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