In-Vitro kinetic release study of illicium verum (Chakraphool) polymeric nanoparticles

Abstract

In traditional medicine, Illicium verum is regarded as the most important plant as it possesses a variety of active components which are responsible for medicinal characteristics such as antifungal, antibacterial, and anti-carminative effects. Nanoparticles have been synthesized from illicium verum employing biodegradable polymer like poly (lactic-co-glycolic acid) PLGA which is widely used as a drug delivery system owing to its two significant properties such as biocompatibility and controlled drug release characteristics. Earlier synthesis, characterization, and antimicrobial studies on these biocompatible nanoparticles were carried out, however, kinetic release models need more instigation. The objective behind this work is to formulate illicium verum loaded PLGA nanoparticles employing solvent evaporation technique and to study kinetic release patterns of the synthesized biocompatible nanoparticles. This work considers the effects of some preparation variables on the size and shape of synthesized nanoparticles and the results show that sonication time, polymer content, the ratio of organic to aqueous phase volume, and the amount of drug have a significant effect on the size of nanoparticles. Polymeric nanoparticles were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) which shows nanoparticles with a size range below 270 nm was successfully prepared. The drug release from drug-loaded nanoparticles was studied by dialysis bag method and the in vitro drug release data was also studied by using various kinetic models. The release profile corresponds to first-order kinetics, specifying that the delivery phase is directly proportional to the absorption of therapeutics in the nanoparticulate, and corresponds to the Higuchi model, indicating medication released by diffusion. The effectiveness of various dosage kinds is controlled by studying the drug's release pattern.