Hydrochlorothiazide containing PLGA nanoparticles: Design, characterization, in-vitro drug release and release kinetic study

Abstract

Nanoscience have become an extended field worldwide because nanomaterials offer various novels and exclusive properties. The properties at the nano scale are dependent on the size, the morphology, and the components. There are various types of nanocarrier systems presently being investigated and explored for drug delivery applications. Polymeric nanoparticles are promising drug delivery systems whose formulation and optimization can be profitably conducted by theoretical methods. The different synthetic polymers are successfully used to prepare polymeric nanoparticles. Poly(lactic-co-glycolic acid) (PLGA) has attracted much attention due to its attractive characteristics such as biodegradability and biocompatibility. The aim of this study was to prepare PLGA nanoparticles containing hydrochlorothiazide by using the emulsion-solvent evaporation method and to study the effect of different formulation variables such as sonication time, polymer content, the amount of surfactant, volume of aqueous phase and drug content. The size and shape of nanoparticles were characterized by Dynamic light scattering (DLS) and transmission electron microscopy (TEM). The Zeta potential study was also performed to understand the surface charge of nanoparticles. The drug release from drug loaded nanoparticles was studied by dialysis bag method and the in vitro drug release data was also studied by various kinetic models. Hopefully we produced spherical shape sustained release hydrochlorothiazide loaded PLGA nanoparticles with a size range under 300 nm with zeta potential–27.9 mV.